Abstract: A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.

Abstract: The invention relates to compositions and methods of treating cystic fibrosis. More specifically, this invention relates to the use the AAV vectors and constructs to provide gene therapy to cystic fibrosis patients.

Abstract: The present invention pertains to methods of inhibiting angiogenesis in an individual in need thereof comprising administering to the individual an agent that inhibits one or more CEP protein adducts wherein the angiogenesis is the result of oxidative peptide modification of polyunsaturated fatty acids (PUFA) in the individual, and administration of the agent inhibits angiogenesis in the individual. In one embodiment, the invention is directed to methods of inhibiting ocular angiogenesis in an individual in need thereof comprising administering to the individual an agent that inhibits the angiogenic activity of one or more CEP protein adducts wherein administration of the. agent inhibits ocular angiogenesis in the individual.

Abstract: Least Square Deconvolution (LSD) uses quantitative allele peak data, for example, allele peak area, allele peak height and optical density, derived/obtained from a sample containing the DNA of more than one contributor to resolve the best-fit genotype profile of each contributor. The resolution is based on finding the least square fit of the mass ratio coefficients at each locus to come closest to the quantitative allele peak data. Consistent top-ranked mass ratio combinations from each locus can be pooled to form at least one composite DNA profile at a subset of the available loci. The top-ranked DNA profiles can be used to check against the profile of a suspect or be used to search for a matching profile in a DNA database.

Abstract: The present invention generally relates to a modified Rubisco large subunit ?N-Methyltransferase (Rubisco LSMT, or RLSMT). The present invention also relates to a modified RLSMT-carbonic anhydrase (RLSMT-CA). This modified RLSMT-CA improves the efficiency of the reduction of CO2 during photosynthesis, which may increase plant growth rates. The present invention also relates to nucleic acids encoding the modified RLSMT-CA or modified RLSMT. Also, the present invention relates to cells including the modified RLSMT-CA or modified RLSMT, plants containing the modified RLSMT-CA or modified RLSMT, and methods using compositions of the present invention. In addition, the present invention relates to antibodies conjugated to CA which may bind to Rubisco, and antibodies which bind a modified RLSMT-CA. The invention also relates to modified forms of the LS and SS of Rubisco where the modified forms are fusions with CA or biologically active fragments thereof.

Abstract: A dihydroxyphenyl cross-linked macromolecular network is provided that is useful in artificial tissue and tissue engineering applications, particularly to provide a synthetic macromolecular network for a wide variety of tissue types. In particular, artificial or synthetic cartilage, vocal cord material, vitreous material, soft tissue material and mitral valve material are described. In an embodiment, the network is composed of tyramine-substituted and cross-linked hyaluronan molecules, wherein cross-linking is achieved via peroxidase-mediated dityramine-linkages that can be performed in vivo. The dityramine bonds provide a stable, coherent hyaluronan-based hydrogel with desired physical properties.

Abstract: The invention provides a method of functionalizing the sidewalls of a plurality of carbon nanotubes with oxygen moieties, the method comprising: exposing a carbon nanotube dispersion to an ozone/oxygen mixture to form a plurality of ozonized carbon nanotubes; and contacting the plurality of ozonized carbon nanotubes with a cleaving agent to form a plurality of sidewall-functionalized carbon nanotubes.

Abstract: An apparatus is provided that includes a light-emitting component, a light-detecting component, a lock-in amplifier, a frequency generator that is operatively linked to the lock-in amplifier and the light-emitting component, a speaker capable of emitting an audio signal wherein the output audio signal varies depending on the detected fluorescence in the sample, and a visible output of relative fluorescent intensity where the visible output varies depending on the detected fluorescence in the sample. The apparatus may also include a laser that is operatively coupled to the lock-in amplifier through a control switch, and focusing lens or an additional type of filter such as an interference filter, a short-pass filter, a notch filter, a long-pass filter or an infrared filter. The apparatus may be used to identify and/or to remove fluorescent or non-fluorescent material from a sample. Associated methods are also disclosed.

Abstract: A method of transferring an electronic material and a method of manufacturing an electronic device using the method of transferring the electronic material. The method of transferring the electronic material includes dipping a template, on which an electronic material layer is formed, into a liquid medium, separating the electronic material layer from the template, and floating the electronic material layer on a surface of the liquid medium; raising up the electronic material layer floated on the surface of the liquid medium by using a target substrate and transferring the electronic material layer on the target substrate; and fixing the electronic material layer to the target substrate.

Abstract: An apparatus for forming a carbon nanotube sheet is provided. The apparatus includes a bath and a driving unit wherein the bath has a bottom surface and is configured to contain a carbon nanotube colloidal solution. The bottom surface is capable of having an array of capillary tubes. The driving unit is configured to drive at least a part of the carbon nanotube colloidal solution out of the bath through the array of capillary tubes.

Abstract: Disclosed is a signal combining apparatus and a resource allocation method using the same in a cellular system using distributed antennas. The resource allocation method includes detecting a channel transmission capacity when a signal is transmitted from a single Relay Station (RS) to a Mobile Station (MS), by a Base Station (BS), detecting a channel transmission capacity when transmitting a signal produced by using signal combining by two or more RSs to the MS, by the BS, selecting a channel transmission capacity with a larger gain between the detected channel transmission capacity of the single RS and the detected channel transmission capacity of the two or more RSs, by the BS; and selecting the signal transmitted by the single RS or the signal produced by using the signal combining by the two or more RSs having the selected channel transmission capacity with the larger gain, and then transmitting the selected signal to the MS.

Abstract: A method of automating a process for controlling a voltage waveform applied to an object is provided. A first waveform for applying to the object is received. A first FFT of the first waveform is calculated. A second waveform for input to the waveform generator is determined based on the first waveform. The determined second waveform is sent to a waveform generator. A third waveform is received that is measured across the object based on a waveform generated by the waveform generator. A second FFT of the received third waveform is calculated. The third waveform is compared with the first waveform to determine a convergence status of the third waveform. If the determined convergence status is not converged, an updated waveform is calculated based on the first FFT and the second FFT and the process is repeated with the updated waveform as the determined second waveform.

Abstract: A method for coding time signals based on generating an asynchronous biphasic pulse train is provided. The method includes generating response signals based upon one or more input signals. A pulse comprises a positive pulse if a voltage of the response signal is greater than a predetermined positive voltage threshold. A pulse comprises a negative pulse if the voltage of the response signal is less than a predetermined negative voltage threshold. The method further includes a method for the reconstruction of a uniformly sampled version of the original signal.

Abstract: An accumulation mode transistor has an impurity concentration of a semiconductor layer in a channel region at a value higher than 2×1017 cm?3 to achieve a large gate voltage swing.

Abstract: The present invention relates to a new composition and methods for preventing the transmission of enterohemorrhagic E. coli and other foodborne pathogens to farm animals. In accordance with one embodiment, a composition comprising lactic acid and acidic calcium sulfate, and a compound selected from the group consisting of caprylic acid, sodium benzoate, butyric acid and chlorine dioxide is provided as an inhibitor of the growth of enterohemorrhagic E. coli and other foodborne pathogens.

Abstract: Code processing for promotional games and the like. In a preferred application, a lottery ticket holder who has purchased a lottery ticket is encouraged to access a designated Internet site by rewarding access thereat on the condition that information visibly supported on the lottery ticket from a given drawing is input at an Internet site and a test indicates that the information so-input matches lottery numbers that were picked for that drawing. Access to predetermined Internet-based services or sites can also be had as an award to an individual for inputting an iconic code at a machine connected to the Internet if the input code tests as valid, with the code being marked so as to be usable only once. Preferably, the iconic code is input by click-selecting displayed icons one after another in an order that corresponds to that of a code provided to the individual. POS methods for code processing are also described.

Abstract: The present invention concerns methods of reducing the antigenicity of a proteinaceous compound while maintaining the compounds biological activity, as well as proteinaceous compositions with biological activity but reduced antigenicity. These methods and compositions have significant benefits to a subject in need of such compounds and compositions. Also included are modified toxin compounds that are truncated and/or possess reduce antigenicity. Such designer toxins have therapeutic, diagnostic, and preventative benefits, particularly as immunotoxins. Methods of treating cancer using these immunotoxins are provided.

Abstract: Techniques for coating a fiber with metal oxide include forming silica in the fiber to fix the metal oxide to the fiber. The coated fiber can be used to facilitate photocatalysis.

Abstract: The invention provides compounds that are A-type procyanidins. The compounds can be prepared by reacting flavylium salts with catechins or analogs thereof, for example, under anhydrous conditions in alcohol.

Abstract: The invention is directed toward a sterile cartilage defect implant material comprising milled lyophilized allograft cartilage pieces ranging from 0.01 mm to 1.0 mm in size in a bioabsorbable carrier taken from a group consisting of sodium hyaluronate, hyaluronic acid and its derivatives, gelatin, collagen, chitosan, alginate, buffered PBS, Dextran or polymers with allogenic chondrocytes or bone marrow cells in an amount exceeding the natural occurrence of same in hyaline cartilage and adding a cell growth additive.