Abstract: This invention provides methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production, AAV producer cells of this invention typically comprise one or more AAV packaging genes, an AAV vector comprising a heterologous (i.e. non-AAV) transgene of interest, and a helper virus such as an adenovirus. The AAV vector preparations produced are generally replication incompetent but are capable of mediating delivery of a transgene of interest (such as a therapeutic gene) to any of a wide variety of tissues and cells. The AAV vector preparations produced according to this invention are also substantially free of helper virus as well as helper viral and cellular proteins and other contaminants. The invention described herein provides methods of producing rAAV particles by culturing producer cells under conditions, such as temperature and pH, that promote release of virus.

Abstract: A method is described for the rapid identification and isolation of cells based on the presence or absence of an ectopically-expressed N-acetyllactosaminide 3-? Galactosyltransferase (?GT) enzyme for the production of ?Galactosyl-(1,3)Galactosyl (?Gal) epitopes on the surface of ?Gal-negative cells. These cells which are genetically modified to express the ?GT enzyme and ?Gal epitopes on glycosylated lipids and proteins of the cell surface are then labeled via an antibody composition which recognizes and binds the ?Gal epitopes on the cell surface. Cells labeled with the anti-?Gal antibody can be isolated by sorting via fluorescence activated cell sorting (FACS), or by magnetic panning techniques. This method is suitable for the rapid positive or negative selection of ?Gal-positive cells from within a population of ?Gal-negative cells without the need to expose cells to antibiotics for any period of time.

Abstract: The invention provides preselected DNA sequences and methods of using them to alter the nutritional content of plant seed. Methods of the invention are directed to increasing the weight percent of an amino acid essential to the diet of animals, or increasing the starch content, of a plant. One method involves stably transforming a cell of a plant with a preselected DNA sequence encoding an RNA molecule substantially identical or complementary to a messenger RNA encoding a plant seed storage protein. An alternative method employs stably transforming cells with at least two preselected DNA sequences, one of which encodes an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, and the other preselected DNA molecule which encodes a preselected polypeptide. The transformed cells are used to generate fertile transgenic plants and seeds, which are characterized by expression of the preselected DNA sequence.

Abstract: Fertile transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants. The process comprises the microjectile bombardment of friable embryogenic callus from the plant to be transformed. The process may be applicable to other graminceous cereal plants which have not proven stably transformable by other techniques.

Abstract: This invention provides transcriptionally-activated AAV ITRs (inverted terminal repeats) which are small and transcriptionally active and uses thereof to optimize the expression of relatively large transgenes packaged in recombinant AAV vectors.

Abstract: AAV vectors may have utility for gene therapy but heretofore a significant obstacle has been the inability to generate sufficient quantities of such recombinant vectors in amounts that would be clinically useful for human gene therapy application. Stable AAV packaging cell lines have been elusive, mainly due to the activities of Rep protein, which down-regulates its own expression and can negatively affect the host cell. This invention provides packaging systems and processes for packaging AAV vectors that effectively circumvent these problems and that allow for substantially increased packaging efficiency.

Abstract: The present invention provides a modified rapid expansion method (termed “low-PBMC-REM” or “modified-REM”), for quickly generating large numbers of T lymphocytes, including cytolytic and helper T lymphocytes, without using the large excesses of peripheral blood mononuclear cells (PBMC) or EBV-transformed lymphoblastoid cells (LCL) characteristic of high-PBMC-REM. Clonal expansions of greater than 500-fold can be achieved within a single stimulation cycle of about 8-14 days.

Abstract: Isolated recombinant polynucleotides comprising elements which promote encapsidation into AAV particles, packaging cells comprising the recombinant polynucleotides, and methods for their use are provided in the present invention. These isolated recombinant polynucleotides comprise a non-AAV ITR encapsidation element (such as the P1 sequence located within the AAV S1 integration site of human chromosome 19) operably linked to one or more heterologous genes to be encapsidated. The constructs may be either integrated into a mammalian cell genome, maintained episomally, or provided transiently.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I244225. This invention thus relates to the plants, seeds and tissue cultures of the variety I244225, and to methods for producing a corn plant produced by crossing a corn plant of variety I244225 with itself or with another corn plant, such as a plant of another variety. This invention furthers relates to corn seeds and plants produced by crossing plants of variety I244225 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I244225, and also to the SSR and isozyme typing profiles of corn variety I244225.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I363128. This invention thus relates to the plants, seeds and tissue cultures of the variety I363128, and to methods for producing a corn plant produced by crossing a corn plant of variety I363128 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I363128 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I363128, and also to the SSR and isozyme typing profiles of corn variety I363128.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I501150. This invention thus relates to the plants, seeds and tissue cultures of the variety I501150, and to methods for producing a corn plant produced by crossing a corn plant of variety I501150 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I501150 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I501150, and also to the SSR and isozyme typing profiles of corn variety I501150.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stablefs transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Abstract: The invention is directed to a method of breeding maize using female plants having a transgene encoding an EPSPS enzyme where the female plants are female reproductively tolerant to treatment with glyphosate but can be made male sterile upon treatment with glyphosate.

Abstract: The invention provides a process to prepare a recombined transgenic Zea mays plant from a transgenic Zea mays plant, wherein the transgene in the recombinant plant has an altered genetic structure relative to the genetic structure of the transgene in the transgenic plant, due to homologous recombination-mediated transgene deletion, amplification or rearrangement.

Abstract: The current invention provides the Zea mays GRP (ZMGRP) promoter. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the ZMGRP promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications.

Abstract: According to the invention, there is provided inbred corn plants, separately designated GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU. This invention thus relates to the plants, seeds and tissue cultures of the fore-mentioned inbred corn plants and to methods for producing a corn plant produced by crossing one of the inbred plants with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing any of the inbred plants GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, O1IBH2, NL054B or FBMU with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plants GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU, and also to the RFLP and genetic isozyme typing profiles of such inbred corn plants.

Abstract: According to the invention, there is provided inbred corn plants, separately designated GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU. This invention thus relates to the plants, seeds and tissue cultures of the fore-mentioned inbred corn plants and to methods for producing a corn plant produced by crossing one of the inbred plants with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing any of the inbred plants GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plants GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASBI, 01IBH2, NL054B and FBMU, and also to the RFLP and genetic isozyme typing profiles of such inbred corn plants.

Abstract: Methods and systems for assessing wide-area performance of a crop variety using a linear mixed model that incorporates geostatistical components and includes parameters for fixed effects, random effects and covariances. A wide-area database is constructed that includes covariate data, spatial coordinates of testing locations of one or more crop varieties, geographic areas in which the testing locations reside, and performance trait values of one or more crop varieties. The parameters for the fixed effects, random effects and covariances are estimated by fitting the linear mixed model with data in the wide-area database. Long-term expected performance of the crop variety may be estimated using the parameter estimates. Average performance of the crop variety for a given time period may be predicted using the parameter estimates.