Abstract: High-efficiency AAV packaging constructs and methods for their use are provided. in the present invention. These high-efficiency packaging constructs comprise an activating element (such as the P1 sequence located within the AAV S1 integration site of human chromosome 19) amplifiably linked to one or more AAV packaging genes. The constructs may be either integrated into a mammalian cell genome or maintained episomally. Use of the high-efficiency AAV packaging vectors of the invention provides for controlled amplifiable production of rAAV vector constructs.

Abstract: Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.

Abstract: A novel soybean cultivar, designated SW84112, is disclosed. The invention relates to the seeds of soybean cultivar SW84112, to the plants of soybean SW84112 and to methods for producing a soybean plant produced by crossing the cultivar SW84112 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar SW84112 with another soybean cultivar.

Abstract: A novel soybean cultivar, designated SW80000, is disclosed. The invention relates to the seeds of soybean cultivar SW80000, to the plants of soybean SW80000 and to methods for producing a soybean plant produced by crossing the cultivar SW80000 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar SW80000 with another soybean cultivar.

Abstract: A novel soybean cultivar, designated SW61302, is disclosed. The invention relates to the seeds of soybean cultivar SW61302, to the plants of soybean SW61302 and to methods for producing a soybean plant produced by crossing the cultivar SW61302 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar SW61302 with another soybean cultivar.

Abstract: Recombinant viral vectors, especially parvovirus vectors such as adeno-associated virus (AAV) vectors, capable of enhanced expression of heterologous sequences, and methods for their construction and use, are provided. The vectors have a structure, or are capable of rapidly adopting a structure, which involves intrastrand base pairing of at least one region in a heterologous sequence.

Abstract: Recombinant vectors are provided that render the proliferative response of activated lymphocytes, particularly cytotoxic T lymphocytes, of lessened dependency on T helper cells. The vectors are comprised of a region encoding a stimulatory factor polypeptide operably linked to a heterologous transcriptional control region. Expression of the stimulatory factor polypeptide from the recombinant polynucleotide in an activated lymphocyte renders the proliferative response of less dependent on lymphocyte T helper cells. When the lymphocyte is activated by binding of its cognate antigen the transcriptional control region causes transcription of the stimulatory factor encoding region. The cells containing the vector, particularly CTLs, are of use in immunotherapy.

Abstract: The present invention provides methods for increasing yield in plants by introducing a gene encoding phosphinothricin acetyltransferase. The invention further involves a method of transferring said increased yield phenotype to other lines of plants by crossing. A maize transformant is identified in which the phosphinothricin acetyltransferase gene integration event is correlated with increased yield.

Abstract: The current invention provides the maize A3 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the A3 promoter directly by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: The invention provides a process to prepare a recombined transgenic Zea mays plant from a transgenic Zea mays plant, wherein the transgene in the recombinant plant has an altered genetic structure relative to the genetic structure of the transgene in the transgenic plant, due to recombination-mediated transgene deletion.

Abstract: A description of artificial transcription factors (ATFs) is provided having a non-peptidic-DNA binding domain, flexible linker and a short synthetic effector domain. The ATFs are highly potent transcriptional modulators in vitro and in vivo. Methods for targeted manipulation of gene expression and the development of new class of pharmaceuticals are also provided.

Abstract: The present invention provides a highly-efficient method for the selection and identification of insertional mutants. In the technique, a non-selective amplification is used to isolate a plurality of insertion events from a population of individuals comprising insertion mutations. Specific insertion events can then be identified from the population by the use of gene specific probes or primers. Through the identification of mutants for a particular gene, data may be obtained regarding the function and phenotypic effects of that gene, and thereby, the gene can be employed in the creation of novel biotechnological products.

Abstract: This invention provides methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production, AAV producer cells of this invention typically comprise one or more AAV packaging genes, an AAV vector comprising a heterologous (i.e. non-AAV) transgene of interest, and a helper virus such as an adenovirus. The AAV vector preparations produced are generally replication incompetent but are capable of mediating delivery of a transgene of interest (such as a therapeutic gene) to any of a wide variety of tissues and cells. The AAV vector preparations produced according to this invention are also substantially free of helper virus as well as helper viral and cellular proteins and other contaminants. The invention described herein provides methods of producing rAAV particles by culturing producer cells under conditions, such as temperature and pH, that promote release of virus.

Abstract: Fertile transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants. The process comprises the microjectile bombardment of friable embryogenic callus from the plant to be transformed. The process may be applicable to other graminaceous cereal plants which have not proven stably transformable by other techniques.

Abstract: The present invention provides AAV “split-packaging” genes, and packaging cells comprising such genes, for use in the production of high titers of replication-incompetent recombinant AAV vectors that can be used to deliver transgenes of interest to a variety of mammalian cells.

Abstract: The present invention provides recombinant adeno-associated virus (rAAV) vectors encoding a tumor necrosis factor (TNF) antagonist and methods using these vectors to reduce levels of TNF in a mammal. The invention also provides methods of using these rAAV vectors in palliating TNF-associated disorders.

Abstract: The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as seeds and progeny derived from these plants, are also provided. The present invention also provides a cDNA sequence of an alpha and a beta subunit of a maize anthranilate synthase.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I362697. This invention thus relates to the plants, seeds and tissue cultures of the variety I362697, and to methods for producing a corn plant produced by crossing a corn plant of variety I362697 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I362697 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I362697, and also to the SSR and isozyme typing profiles of corn variety I362697.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I181664. This invention thus relates to the plants, seeds and tissue cultures of the variety I181664, and to methods for producing a corn plant produced by crossing a corn plant of variety I181664 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I181664 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I181664, and also to the SSR and isozyme typing profiles of corn variety I181664.

Abstract: Fertile transgenic Zea mays (corn) plants which stably express recombinant DNA which is heritable are provided wherein said DNA preferably comprises a recombinant gene which encodes a seed storage protein, so that the amino acid profile of the corn is improved.