Patents Assigned to Genomatica, Inc.
  • Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone
    Patent number: 9988656
    Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.
    Type: Grant
    Filed: November 30, 2015
    Date of Patent: June 5, 2018
    Assignee: Genomatica, Inc.
    Inventors: Jun Sun, Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Wei Niu, John D. Trawick, Robert Haselbeck
  • Microorganisms and methods for producing alkenes
    Patent number: 9988648
    Abstract: The invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. The invention additionally provides methods of using such microbial organisms to produce an alkene, by culturing a non-naturally occurring microbial organism containing an alkene pathway as described herein under conditions and for a sufficient period of time to produce an alkene.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: June 5, 2018
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Robin E. Osterhout
  • Microorganisms for producing 1,3-butanediol and methods related thereto
    Patent number: 9982281
    Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: May 29, 2018
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Jun Sun, Priti Pharkya
  • ENGINEERED MICROORGANSIMS & METHODS FOR IMPROVED CROTYL ALCOHOL PRODUCTION
    Publication number: 20180135082
    Abstract: Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.
    Type: Application
    Filed: April 7, 2016
    Publication date: May 17, 2018
    Applicant: GENOMATICA, iNC.
    Inventors: Stephanie J. Culler, Nicholas Eakley, Kevin Gregory Hoff, Robin E. Osterhout, Priti Pharkya, Erik C. Ralph, Stephen Van Dien
  • Microorganisms for the production of methacrylic acid
    Patent number: 9951355
    Abstract: The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.
    Type: Grant
    Filed: December 1, 2014
    Date of Patent: April 24, 2018
    Assignee: GENOMATICA, INC.
    Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
  • Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing succinate related thereto
    Patent number: 9932611
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.
    Type: Grant
    Filed: October 21, 2013
    Date of Patent: April 3, 2018
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,2-propanediol, n-propanol, 1,3-propanediol, or glycerol related thereto
    Patent number: 9909150
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.
    Type: Grant
    Filed: November 4, 2013
    Date of Patent: March 6, 2018
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
    Patent number: 9885064
    Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: February 6, 2018
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Christophe H. Schilling, Anthony P. Burgard, John D. Trawick
  • Process and systems for obtaining 1,4-butanediol from fermentation broths
    Patent number: 9873652
    Abstract: A process of purifying 1,4-butanediol (1,4-BDO) from a fermentation broth including separating solid materials, salts and water, and subjecting the resulting material to a two, three or four column distillation system, that can include a wiped film evaporator to produce a purified 1,4-butanediol product.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: January 23, 2018
    Assignee: Genomatica, Inc.
    Inventors: SVB Janardhan Garikipati, Michael Japs, Ishmael M. Sonico
  • Microorganisms and methods for the biosynthesis of butadiene
    Patent number: 9732361
    Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.
    Type: Grant
    Filed: October 21, 2013
    Date of Patent: August 15, 2017
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Anthony P. Burgard, Jun Sun, Robin E. Osterhout, Priti Pharkya
  • Microorganisms and methods for the production of caprolactone
    Patent number: 9719118
    Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.
    Type: Grant
    Filed: January 13, 2016
    Date of Patent: August 1, 2017
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya, Mark J. Burk
  • Organisms for the production of 1,3-butanediol
    Patent number: 9708632
    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: July 18, 2017
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
  • MICROORGANISMS AND METHODS FOR THE PRODUCTION OF BUTADIENE USING ACETYL-COA
    Publication number: 20170191085
    Abstract: The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.
    Type: Application
    Filed: July 2, 2015
    Publication date: July 6, 2017
    Applicant: Genomatica, Inc.
    Inventors: Priti PHARKYA, Anthony P. BURGARD, Mark J. BURK
  • Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
    Patent number: 9689006
    Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.
    Type: Grant
    Filed: May 19, 2015
    Date of Patent: June 27, 2017
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Anthony P. Burgard, Priti Pharkya
  • Microorganisms and methods for production of 4-hydroxybutyrate, 1,4-butanediol and related compounds
    Patent number: 9677045
    Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.
    Type: Grant
    Filed: June 3, 2013
    Date of Patent: June 13, 2017
    Assignee: Genomatica, Inc.
    Inventors: Priti Pharkya, Anthony P. Burgard, Stephen J. Van Dien, Robin E. Osterhout, Mark J. Burk, John D. Trawick, Michael P. Kuchinskas, Brian Steer
  • Preparation of 6-aminocaproic acid from 5-formyl valeri C acid
    Patent number: 9663805
    Abstract: The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam.
    Type: Grant
    Filed: December 13, 2013
    Date of Patent: May 30, 2017
    Assignee: Genomatica, Inc.
    Inventors: Petronella Catharina Raemakers-Franken, Martin Schurmann, Axel Christoph Trefzer, Stefaan Marie Andre De Wildeman
  • Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,4-butanediol related thereto
    Patent number: 9657316
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.
    Type: Grant
    Filed: August 26, 2013
    Date of Patent: May 23, 2017
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Semi-synthetic terephthalic acid via microorganisms that produce muconic acid
    Patent number: 9562241
    Abstract: The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate.
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: February 7, 2017
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Robin E. Osterhout, Jun Sun
  • Method for producing polybutylene terephthalate
    Patent number: 9556307
    Abstract: The present invention aims to provide a method for producing polybutylene terephthalate (PBT) with an excellent color using biomass-derived 1,4-butanediol (BG). The invention relates to a method for producing PBT comprising a step of subjecting a diol component containing raw material 1,4-BG having a nitrogen content of 0.01 to 50 ppm by mass and a dicarboxylic acid component to esterification or ester-exchange reaction, and a polycondensation reaction step for obtaining PBT from the reactant, wherein the content of gamma butyrolactone in the raw material 1,4-BG is 1 to 100 ppm by mass.
    Type: Grant
    Filed: December 4, 2014
    Date of Patent: January 31, 2017
    Assignees: MITSUBISHI CHEMICAL CORPORATION, Genomatica, Inc.
    Inventors: Masaru Utsunomiya, Yusuke Izawa, Norikazu Konishi, Shinichiro Matsuzono, Takayuki Suzuki, Michael Japs, Mark Burk, Warren Clark
  • Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene
    Patent number: 9556461
    Abstract: The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: January 31, 2017
    Assignee: Genomatica, Inc.
    Inventors: Robin E. Osterhout, Anthony P. Burgard, Priti Pharkya, Mark J. Burk