Patents Assigned to Handylab, Inc.
  • Patent number: 10443088
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: October 15, 2019
    Assignee: HandyLab, Inc.
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Patent number: 10364456
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Grant
    Filed: October 3, 2014
    Date of Patent: July 30, 2019
    Assignee: HANDYLAB, INC.
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Patent number: 10351901
    Abstract: A microfluidic processing device includes a substrate defining a microfluidic network. The substrate is in thermal communication with a plurality of N independently controllable components and a plurality of input output contacts for connecting the substrate to an external controller. Each component has at least two terminals. Each terminal is in electrical communication with at least one contact. The number of contacts required to independently control the N components is substantially less than the total number of terminals. Upon actuation, the components typically heat a portion of the microfluidic network and/or sense a temperature thereof.
    Type: Grant
    Filed: June 12, 2017
    Date of Patent: July 16, 2019
    Assignee: HandyLab, Inc.
    Inventors: Karthik Ganesan, Kalyan Handique
  • Patent number: 10234474
    Abstract: The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides. The technology still more particularly relates to automated devices for carrying out pipetting operations, particularly on samples in parallel, consistent with sample preparation and delivery of PCR-ready nucleotide extracts to a cartridge wherein PCR is run.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: March 19, 2019
    Assignee: HandyLab, Inc.
    Inventors: Jeff Williams, Kerry Wilson
  • Patent number: 10179910
    Abstract: A rack for holding samples and various reagents, wherein the rack may be used for loading the samples and reagents prior to using the reagents. The rack accepts complementary reagent holders, each of which contain a set of reagents for carrying out a predetermined processing operation, such as preparing biological samples for amplifying and detecting polynucleotides extracted from the samples.
    Type: Grant
    Filed: February 8, 2016
    Date of Patent: January 15, 2019
    Assignee: HandyLab, Inc.
    Inventors: Patrick Duffy, Kerry Wilson, Kalyan Handique, Jeff Williams
  • Patent number: 10139012
    Abstract: An apparatus for providing thermal and magnetic energy to a receptacle containing a reaction mixture and a magnetic retention member. The apparatus can also control heating of a reaction mixture, and bring about a separation of magnetic particles from the reaction mixture. The reaction mixture typically comprises polynucleotides from a biological sample that are being brought into a PCR-ready form.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: November 27, 2018
    Assignee: HandyLab, Inc.
    Inventor: Kalyan Handique
  • Patent number: 10100302
    Abstract: Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: October 16, 2018
    Assignee: HANDYLAB, INC.
    Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig
  • Patent number: 10071376
    Abstract: The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.
    Type: Grant
    Filed: November 13, 2015
    Date of Patent: September 11, 2018
    Assignee: HANDYLAB, INC.
    Inventors: Jeff Williams, Kerry Wilson, Kalyan Handique
  • Patent number: 10065185
    Abstract: The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.
    Type: Grant
    Filed: January 15, 2016
    Date of Patent: September 4, 2018
    Assignee: HandyLab, Inc.
    Inventor: Kalyan Handique
  • Patent number: 9815057
    Abstract: The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.
    Type: Grant
    Filed: April 28, 2014
    Date of Patent: November 14, 2017
    Assignee: HandyLab, Inc.
    Inventor: Kalyan Handique
  • Patent number: 9802199
    Abstract: The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.
    Type: Grant
    Filed: November 10, 2014
    Date of Patent: October 31, 2017
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra
  • Patent number: 9701957
    Abstract: A holder for reagents, such as may be used for transporting the reagents and for carrying out processing operations on biological samples with the reagents. The holders typically hold reagents for amplifying polynucleotides extracted from the samples. The holder comprises a connecting member; a process tube affixed to the connecting member and having an aperture located in the connecting member; at least one socket, located in the connecting member, and configured to accept a pipette tip; two or more reagent tubes disposed on the underside of the connecting member, each having an inlet aperture located in the connecting member; and one or more receptacles, located in the connecting member and each being configured to receive a reagent tube. Also described are reagent tubes configured with stellated shaped patterns, on their bottom interior surfaces, configured to facilitate complete or near-complete withdrawal of fluid from the tube, via a pipette tip.
    Type: Grant
    Filed: July 14, 2008
    Date of Patent: July 11, 2017
    Assignee: HandyLab, Inc.
    Inventors: Kerry Wilson, Kalyan Handique, Sundaresh N. Brahmasandra, Jeff Williams
  • Patent number: 9677121
    Abstract: A microfluidic processing device includes a substrate defining a microfluidic network. The substrate is in thermal communication with a plurality of N independently controllable components and a plurality of input output contacts for connecting the substrate to an external controller. Each component has at least two terminals. Each terminal is in electrical communication with at least one contact. The number of contacts required to independently control the N components is substantially less than the total number of terminals. Upon actuation, the components typically heat a portion of the microfluidic network and/or sense a temperature thereof.
    Type: Grant
    Filed: November 21, 2014
    Date of Patent: June 13, 2017
    Assignee: HandyLab, Inc.
    Inventors: Karthik Ganesan, Kalyan Handique
  • Patent number: 9670528
    Abstract: A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
    Type: Grant
    Filed: March 24, 2014
    Date of Patent: June 6, 2017
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Gene Parunak, Aaron Kehrer, Betty Wu, Karthik Ganesan
  • Patent number: 9618139
    Abstract: An apparatus for providing thermal and magnetic energy to a receptacle containing a reaction mixture and a magnetic retention member. The apparatus can also control heating of a reaction mixture, and bring about a separation of magnetic particles from the reaction mixture. The reaction mixture typically comprises polynucleotides from a biological sample that are being brought into a PCR-ready form.
    Type: Grant
    Filed: July 23, 2008
    Date of Patent: April 11, 2017
    Assignee: Handylab, Inc.
    Inventor: Kalyan Handique
  • Patent number: 9528142
    Abstract: Systems and methods for preventing or reducing unwanted heat in a microfluidic device while generating heat in selected regions of the device are described. Current can be supplied to a heating element through electric leads that are designed so that the current density in the leads is substantially lower than the current density in the heating element. Unwanted heat in the microfluidic complex can be reduced by thermally isolating the electric leads from the microfluidic complex by, for example, running each lead directly away from the microfluidic complex. Unwanted heat can be removed from selected regions of the microfluidic complex using one or more cooling devices.
    Type: Grant
    Filed: June 5, 2015
    Date of Patent: December 27, 2016
    Assignee: HandyLab, Inc.
    Inventor: Kalyan Handique
  • Patent number: 9512479
    Abstract: A method and apparatus are provided for identifying a biological sample obtained during either paternity screening, genetic screening, prenatal diagnosis, presymptomatic diagnosis, diagnosis to detect the presence of a target microorganism carrier detection analysis, forensic chemical analysis, or diagnosis of a subject to determine whether a subject is afflicted with a particular disease or disorder, or is at risk of developing a particular disorder, wherein the result obtained from the analysis is associated with the unique DNA fingerprint biological barcode of the genotype of the subject being analyzed. The methods and apparatus of the invention have application in the fields of diagnostic medicine, disease diagnosis in animals and plants, identification of genetically inherited diseases in humans, family relationship analysis, forensic analysis, and microbial typing.
    Type: Grant
    Filed: December 1, 2008
    Date of Patent: December 6, 2016
    Assignee: HandyLab, Inc.
    Inventors: Purnima Kurnool, Betty Bei Yue Wu, Peter M. Banks
  • Patent number: 9347586
    Abstract: The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides. The technology still more particularly relates to automated devices for carrying out pipetting operations, particularly on samples in parallel, consistent with sample preparation and delivery of PCR-ready nucleotide extracts to a cartridge wherein PCR is run.
    Type: Grant
    Filed: October 15, 2012
    Date of Patent: May 24, 2016
    Assignee: HandyLab, Inc.
    Inventors: Jeff Williams, Kerry Wilson
  • Publication number: 20160096178
    Abstract: The present technology provides for a heater substrate that contains networks of heater elements configured to controllably and selectively deliver heat to one or more PCR reaction chambers in a microfluidic substrate with which the heater substrate makes contact. In exemplary embodiments, the heater substrate can deliver heat to 12, 24, 48, or 96 chambers independently of one another, or simultaneously. The heater substrate is located in a heater unit that may be introduced into a diagnostic apparatus that can receive and position a microfluidic substrate, such as in a cartridge, in contact with the heater unit, receive one or more polynucleotide containing samples into one or more lanes in the microfluidic substrate, and cause amplification of the polynucleotides to occur, and detect presence of absence of specified polynucleotides in the amplified samples.
    Type: Application
    Filed: December 30, 2011
    Publication date: April 7, 2016
    Applicant: HandyLab, Inc.
    Inventor: Kalyan Handique
  • Patent number: D787087
    Type: Grant
    Filed: February 8, 2016
    Date of Patent: May 16, 2017
    Assignee: HandyLab, Inc.
    Inventors: Patrick Duffy, Kerry Wilson, Kalyan Handique, Jeff Williams