Patents Assigned to Handylab, Inc.
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Patent number: 9186677Abstract: The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.Type: GrantFiled: July 14, 2008Date of Patent: November 17, 2015Assignee: HANDYLAB, INC.Inventors: Jeff Williams, Kerry Wilson, Kalyan Handique
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Patent number: 9080207Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.Type: GrantFiled: December 3, 2012Date of Patent: July 14, 2015Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Sundaresh N Brahmasandra, Karthik Ganesan, Jeff Williams
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Patent number: 9051604Abstract: Systems and methods for preventing or reducing unwanted heat in a microfluidic device while generating heat in selected regions of the device are described. Current can be supplied to a heating element through electric leads that are designed so that the current density in the leads is substantially lower than the current density in the heating element. Unwanted heat in the microfluidic complex can be reduced by thermally isolating the electric leads from the microfluidic complex by, for example, running each lead directly away from the microfluidic complex. Unwanted heat can be removed from selected regions of the microfluidic complex using one or more cooling devices.Type: GrantFiled: May 23, 2014Date of Patent: June 9, 2015Assignee: HandyLab, Inc.Inventor: Kalyan Handique
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Patent number: 9040288Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.Type: GrantFiled: March 26, 2007Date of Patent: May 26, 2015Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Betty Wu, Nikhil Phadke, Gene Parunak, Jeff Williams
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Patent number: 9028773Abstract: A system and method for reducing the number of input/output connections required to connect a microfluidic substrate to an external controller for controlling the substrate. In one example, a microfluidic processing device is fabricated on a substrate having a plurality of N independently controllable components, (e.g., a resistive heating elements) each having at least two terminals. The substrate includes a plurality of input/output contacts for connecting the substrate to an external controller, and a plurality of leads for connecting the contacts to the terminals of the components. The leads are arranged to allow the external controller to supply control signals to the terminals of the components via the contacts using substantially fewer contacts than the total number of component terminals.Type: GrantFiled: March 28, 2014Date of Patent: May 12, 2015Assignee: Handylab, Inc.Inventor: Karthik Ganesan
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Patent number: 8894947Abstract: A microfluidic processing device includes a substrate defining a microfluidic network. The substrate is in thermal communication with a plurality of N independently controllable components and a plurality of input output contacts for connecting the substrate to an external controller. Each component has at least two terminals. Each terminal is in electrical communication with at least one contact. The number of contacts required to independently control the N components is substantially less than the total number of terminals. Upon actuation, the components typically heat a portion of the microfluidic network and/or sense a temperature thereof.Type: GrantFiled: March 19, 2013Date of Patent: November 25, 2014Assignee: HandyLab, Inc.Inventors: Karthik Ganesan, Kalyan Handique
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Patent number: 8895311Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.Type: GrantFiled: September 18, 2002Date of Patent: November 25, 2014Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Karthik Ganesan, Sundaresh N. Brahmasandra
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Publication number: 20140342352Abstract: Systems and methods for preventing or reducing unwanted heat in a microfluidic device while generating heat in selected regions of the device are described. Current can be supplied to a heating element through electric leads that are designed so that the current density in the leads is substantially lower than the current density in the heating element. Unwanted heat in the microfluidic complex can be reduced by thermally isolating the electric leads from the microfluidic complex by, for example, running each lead directly away from the microfluidic complex. Unwanted heat can be removed from selected regions of the microfluidic complex using one or more cooling devices.Type: ApplicationFiled: May 23, 2014Publication date: November 20, 2014Applicant: HANDYLAB, INC.Inventor: KALYAN HANDIQUE
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Patent number: 8883490Abstract: The present technology provides for an fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.Type: GrantFiled: November 14, 2007Date of Patent: November 11, 2014Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Sundaresh N. Brahmasandra
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Publication number: 20140329301Abstract: Disclosed herein are devices configured for the amplification and detection of multiple targets from a sample, and methods of using the same. The devices disclosed herein comprise microfluidic cartridges have a first stage (amplification) and a second (detection) stage. The two-stage design of the cartridges enables testing for multiple targets within a sample, i.e., from a single nucleic acid amplification reaction. Methods for the amplification and detection of a plurality of target nucleic acids from a sample are also disclosed herein.Type: ApplicationFiled: May 1, 2014Publication date: November 6, 2014Applicant: Handylab, Inc.Inventor: Kalyan Handique
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Publication number: 20140323711Abstract: Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.Type: ApplicationFiled: April 25, 2014Publication date: October 30, 2014Applicant: HANDYLAB, INC.Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig
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Publication number: 20140323357Abstract: The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.Type: ApplicationFiled: April 28, 2014Publication date: October 30, 2014Applicant: HANDYLAB, INC.Inventor: Kalyan Handique
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Patent number: 8852862Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.Type: GrantFiled: November 16, 2005Date of Patent: October 7, 2014Assignee: HandyLab, Inc.Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
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Publication number: 20140297047Abstract: A system and method for reducing the number of input/output connections required to connect a microfluidic substrate to an external controller for controlling the substrate. In one example, a microfluidic processing device is fabricated on a substrate having a plurality of N independently controllable components, (e.g., a resistive heating elements) each having at least two terminals. The substrate includes a plurality of input/output contacts for connecting the substrate to an external controller, and a plurality of leads for connecting the contacts to the terminals of the components. The leads are arranged to allow the external controller to supply control signals to the terminals of the components via the contacts using substantially fewer contacts than the total number of component terminals.Type: ApplicationFiled: March 28, 2014Publication date: October 2, 2014Applicant: HANDYLAB, INC.Inventor: Karthik Ganesan
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Publication number: 20140227710Abstract: The present invention relates to a system and method for moving samples, such as fluid, within a microfluidic system using a plurality of gas actuators for applying pressure at different locations within the microfluidic. The system includes a substrate which forms a fluid network through which fluid flows, and a plurality of gas actuators integral with the substrate. One such gas actuator is coupled to the network at a first location for providing gas pressure to move a microfluidic sample within the network. Another gas actuator is coupled to the network at a second location for providing gas pressure to further move at least a portion of the microfluidic sample within the network. A valve is coupled to the microfluidic network so that, when the valve is closed, it substantially isolates the second gas actuator from the first gas actuator.Type: ApplicationFiled: April 17, 2014Publication date: August 14, 2014Applicant: HandyLab, Inc.Inventors: Kalyan Handique, Gene Parunak
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Publication number: 20140212882Abstract: A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.Type: ApplicationFiled: March 24, 2014Publication date: July 31, 2014Applicant: HANDYLAB, INC.Inventors: Kalyan Handique, Gene Parunak, Aaron Kehrer, Betty Wu, Karthik Ganesan
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Patent number: 8768517Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.Type: GrantFiled: June 24, 2013Date of Patent: July 1, 2014Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Karthik Ganesan, Sundaresh N. Brahmasandra
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Patent number: 8765076Abstract: The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.Type: GrantFiled: November 14, 2007Date of Patent: July 1, 2014Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Gene Parunak, Cecelia Haley
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Patent number: 8734733Abstract: The present relates to a system and method for preventing or reducing unwanted heat in a microfluidic of the device while generating heat in selected regions of the device. Current can be supplied to a heating element through electric leads that are designed so that the current density in the leads is substantially lower than the current density in the heating element. Unwanted heat in the microfluidic complex can be reduced by thermally isolating the electric leads from the microfluidic complex by, for example, running each lead directly away from the microfluidic complex. Unwanted heat can be removed from selected regions of the microfluidic complex using one or more cooling devices.Type: GrantFiled: May 13, 2013Date of Patent: May 27, 2014Assignee: Handylab, Inc.Inventor: Kalyan Handique
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Patent number: 8710211Abstract: Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.Type: GrantFiled: December 3, 2012Date of Patent: April 29, 2014Assignee: Handylab, Inc.Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig