Abstract: Genetically modified Zymomonas mobilis to utilize inorganic N source, methods and uses thereof are provided. The genetically modified Z. mobilis has a genome with the knockout of a locus ZMO1107 from Z. mobilis ZM4 could ferment inorganic N source.

Abstract: The present disclosure provides a hydraulic phosphogypsum (PG)-based cementitious material, and a preparation method and use thereof, belonging to the technical field of cementitious materials. The present disclosure provides a hydraulic PG-based cementitious material, including the following raw materials: modified PG particles and an auxiliary active powder, where the modified PG particles have a dosage of 50 wt % to 95 wt %; the modified PG particles are obtained by conducting modification on original PG particles through a calcareous material, and the calcareous material has a mass 3% to 5% of that of the original PG particles; and the original PG particles have typical dimensions of: a length of 50 ?m to 200 ?m and an aspect ratio of 1.5 to 5; and at least 80% of materials in the auxiliary active powder have a particle size of less than or equal to 60 ?m. In the present disclosure, the hydraulic PG-based cementitious material adopts a particle accumulation method of gap grading.

Abstract: Genetically modified Zymomonas mobilis to utilize inorganic N source, methods and uses thereof are provided. The genetically modified Z. mobilis has a genome with the knockout of a locus ZMO1107 from Z. mobilis ZM4 could ferment inorganic N source.

Abstract: A method and a device for preparing MoF6 based on plasma activation of SF6. The method includes the following steps: S1, filling a discharge area of a plasma reactor with molybdenum powder; S2, introducing an inert gas and an SF6 gas into the discharge area of the plasma reactor, where the inert gas is ionized into plasma, and the SF6 gas is ionized into fluorine atoms and low-fluorine sulfides after being activated by the plasma; and S3, generating an MoF6 gas and sulfur elements through reaction of the fluorine atoms and the low-fluorine sulfides with the molybdenum powder.

Abstract: A non-sintered high-strength lightweight aggregate one-shot prepared from sulfur-based and alkaline-based solid wastes by stirring, granulation, and foaming, and a preparation method therefor and use thereof. The non-sintered high-strength lightweight aggregate is prepared from a sulfur-based solid waste, an alkaline-based solid waste, an auxiliary cementing material, a ferro-aluminum-sulfur cementing material, water, and a foaming agent as raw materials. Based on the mass of the total solid, the total content of the sulfur-based solid waste, the alkaline-based solid waste, and the auxiliary cementing material is 80-90 wt %, and the content of the ferro-aluminum-sulfur cementing material is 10-20 wt %. The mass ratio of the water to the total solid is (15-20):(80-85). The foaming agent accounts for 0.3-0.7% of the mass of the total solid. The mass ratio between the sulfur-based solid waste, the alkaline-based solid waste, and the auxiliary cementing material is (27-33):(27-33):(18-25).

Abstract: Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof are provided. The recombinant Zymomonas mobilis carries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.

Abstract: The invention discloses a low power consumption and high precision resistance-free CMOS reference voltage source circuit, which includes a, a positive temperature coefficient voltage generation circuit and a starting circuit. The self-bias current source circuit uses two NMOS tubes with different threshold voltages in the subthreshold region to form a stack structure, which generates the bias current and negative temperature coefficient voltage on the order of nanoampere. The positive temperature coefficient voltage generation circuit uses PMOS differential to generate positive temperature coefficient voltage for the structure and performs first-order curvature compensation for negative temperature coefficient voltage.

Abstract: Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof are provided. The recombinant Zymomonas mobilis carries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.

Abstract: A Pyrococcus furiosus Argonaute (PfAgo) mutant protein with mesophilic target nucleic acid cleavage activity and an application thereof are provided. The PfAgo mutant protein has an amino acid mutation on at least one of a 617th position and a 618th position relative to the sequence shown in SEQ ID NO: 1, specifically, K617E/G and/or L618Y/F/W/G. Compared with a wild-type PfAgo protein, the provided PfAgo mutant protein has activity in the temperature range of 30-95 Celsius degree (° C.), effectively expanding application range of prokaryotic Argonaute (pAgo) protein.

Abstract: An application of a prokaryotic Argonaute protein with only a cleavage activity of a target ribonucleic acid (RNA) in RNA editing is provided. The Argonaute protein is derived from a mesophilic prokaryotes Verrucomimicrobia bacterium, and its amino acid sequence is shown in SEQ ID NO: 1 or a protein with high similarity to SEQ ID NO: 1 and the same function. This protein has binding activity to a single-stranded guide DNA and nuclease activity only to the target RNA complementarily paired with the single-stranded guide DNA. Therefore, the protein can be utilized for in vitro and in vivo targeted RNA editing, providing a new powerful tool for RNA editing.

Abstract: A visible light positioning (VLP)-assisted multi-user optical communication system includes a micro base station and a user receiver; the micro base station includes a mounting plate, a visible light locator is provided at a middle of a bottom surface of the mounting plate, the bottom surface of the mounting plate is provided with a plurality of automatic tracking optical communication devices surrounding around the visible light locator; the visible light locator is connected to the plurality of automatic tracking optical communication devices through a serial bus; a micro base station ID and a current working status data of the automatic tracking optical communication devices are cyclically broadcast to a coverage area through the visible light locator; a user location coordinate and a user identification are sent to the visible light locator by the user receiver.

Abstract: The present disclosure provides a method for induced differentiation of an extended pluripotent stem cell (EPSC) into a cardiomyocyte and use thereof, and belongs to the technical field of biomedicine. A reagent used for the induced differentiation is a culture medium having definite chemical components, which can obtain cardiomyocytes having high purity and stable between-batch differentiation efficiency. Compared with cardiomyocytes differentiated from existing pluripotent stem cells, the cardiomyocyte obtained has strong early proliferation ability, and more cell number can be obtained; after extended culture and construction into engineering heart microtissue, maturity is higher, the structure is more regularly arranged, and functional contractility is enhanced. Therefore, the present disclosure is a suitable for mechanism research of heart diseases, drug screening, and cell therapy, and thus has excellent practical application value.

Abstract: The present invention provides quaternary ammonium cyclodextrin and a preparation method and uses thereof, as well as a silver nanoparticle-cyclodextrin complex and a preparation method and uses thereof. The quaternary ammonium cyclodextrin of the present invention is introduced with an amine group and quaternary ammonium groups, while retaining the special structure and properties of cyclodextrin itself. The amine group contained in the structure plays a role in reducing and complexing Ag+ in the synthesis of AgNPs, and plays a certain role in stabilizing nanoparticles and forming a complex in combination with quaternary ammonium groups.

Abstract: The present disclosure provides a method for induced differentiation of an extended pluripotent stem cell (EPSC) into a cardiomyocyte and use thereof, and belongs to the technical field of biomedicine. A reagent used for the induced differentiation is a culture medium having definite chemical components, which can obtain cardiomyocytes having high purity and stable between-batch differentiation efficiency. Compared with cardiomyocytes differentiated from existing pluripotent stem cells, the cardiomyocyte obtained has strong early proliferation ability, and more cell number can be obtained; after extended culture and construction into engineering heart microtissue, maturity is higher, the structure is more regularly arranged, and functional contractility is enhanced. Therefore, the present disclosure is a suitable for mechanism research of heart diseases, drug screening, and cell therapy, and thus has excellent practical application value.

Abstract: The present invention discloses a method of preparing an alkali activation material by using red mud-based wet grinding and carbon sequestration and an application thereof. The preparation method includes: (1) adding water, red mud, a crystalline control agent, and a grinding aid into a wet grinding carbon sequestration apparatus to perform wet grinding, and simultaneously introducing CO2 until a slurry pH reaches 7 to 7.5; and removing wet grinding balls by a sieve to obtain a slurry A; (2) adding carbide slag, water and a water reducer to a wet planetary ball grinder tank for wet grinding, and removing wet grinding balls by a sieve to obtain a slurry B; (3) taking 50 to 80 parts of the slurry A and 20 to 50 parts of the slurry B and mixing them to obtain an alkali activation material.

Abstract: Application of glutamate dehydrogenase GdhA of Peptostreptococcus asaccharolyticus in increasing the yield of poly-?-glutamic acid from Bacillus licheniformis. The glutamate dehydrogenase GdhA of the Bacillus licheniformis WX-02 per se is replaced with the glutamate dehydrogenase derived from the Peptostreptococcus asaccharolyticus by means of homologous recombination, which significantly increases the level of synthesizing the poly-?-glutamic acid for the Bacillus licheniformis, and the yield of the obtained poly-?-glutamic acid from strains is increased at least by more than 20% compared with control strains.

Abstract: A modified cutinase is disclosed. The cutinase has the modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of asparagine at position 181 with alanine, or substitutions of asparagine at position 181 with alanine and phenylalanine at position 235 with leucine. The modified enzyme has improved PET-hydrolytic activity, and thus, the high-activity PET hydrolase is obtained, and the industrial application value of the PET hydrolase is enhanced.

Abstract: A genetically engineered strain of Z. mobilis and uses thereof are provided. The genetically engineered strain named ZMNP is obtained by knockouts of four endogenous plasmids of pZM32, pZM32, pZM36, pZM33, and pZM39 from strain ZM4-Cas12a. ZMNP contains a reduced genome and has a series of excellent traits, such as high transformation efficiency, enhanced tolerance to inhibitors, improved capability to use secondary mother liquor and other excellent performance. ZMNP can be used as a chassis cell to construct diverse cell factories for biochemical production using different feedstocks especially the lignocellulosic biomass.

Abstract: Disclosed are a device of complex gas mixture detection based on optical-path-adjustable spectrum detection and a method therefor, and the device includes: a light source configured for generating an incident beam and emitting the incident beam into an optical gas cell; the optical gas cell, including a cavity configured for accommodating a gas sample, and a reflection module group configured for reflecting the incident beam and a track arranged in the cavity, where the track is consistent with a light path of the light beam in the cavity; a detector module that is connected with the track in a relatively movable manner and is configured for receiving light beams and obtaining spectral data, where an optical path is changed by moving the detector module relative to the track; and a data acquisition unit that is configured for acquiring the spectral data obtained by the detector module.

Abstract: The present invention provides a method of preparing a recycled cementitious material by a phosphogypsum-assisted carbon sequestration pretreatment process. The method includes: (1) placing 100 mass parts of phosphogypsum, 1 to 2 mass parts of grinding aid, 10 to 20 mass parts of sodium-containing alkali component, 150 to 300 mass parts of zirconia balls, and 150 to 300 mass parts of water into a wet grinding tank for wet grinding. After 10 min to 30 min of wet grinding, introducing CO2 at a flow rate of 1.5 to 2.2 mass parts/min to keep a temperature of a wet grinding slurry below 40° C. When the wet grinding slurry reaches pH=10, ending the wet grinding and sieving out a wet grinding slurry; (2) mixing the wet grinding slurry with 700 to 1000 mass parts of slag and 100 to 350 mass parts of water to obtain a recycled cementitious material.