Abstract: An efficient synthesis and assembly method for the large fragment DNA based on the programmable nuclease Argonaute, specifically includes: constructing and treating antibiotic resistance gene reconstructed vectors with linearization, dividing a target DNA into multiple small DNA fragments and then synthesizing the small DNA fragments, followed by loading the synthesized small DNA fragments to the antibiotic resistance gene reconstructed vectors; and the SLIC and resistance gene reconstruction are used to achieve assembly of the target DNA. The method combines the SLIC with a resistance gene reconstruction strategy, allowing for the assembly of 5-6 small fragments in a single resistance gene reconstruction, which is more efficient and time-saving. Moreover, the number of the resistance gene reconstructions can be flexibly chosen according to the length of the DNA fragments. Mutations are not introduced caused by PCR, and the reconstructed large fragment do not need a second sequencing, saving time and costs.

Abstract: The present disclosure provides a system for CO2 storage and utilization of a concrete mixing plant and a method thereof. The system includes a slurry making module, a cyclic grinding module and a material storage and application module. The cyclic grinding module can grind, in the presence of a gas containing CO2 and medium balls, a composite slurry prepared by the slurry making module, and can cyclically use the gas containing CO2, so as to realize continuous production of a carbon-mineralized slurry. The material storage and application module includes a material storage bin and a mixing machine connected with each other, and the material storage bin is configured to receive and store the carbon-mineralized slurry from a slurry bin. The mixing machine is configured to mix and prepare the carbon-mineralized slurry into a commodity concrete.

Abstract: The present disclosure provides a system for CO2 storage and utilization of a concrete mixing plant and a method thereof. The system includes a slurry making module, a cyclic grinding module and a material storage and application module. The cyclic grinding module can grind, in the presence of a gas containing CO2 and medium balls, a composite slurry prepared by the slurry making module, and can cyclically use the gas containing CO2, so as to realize continuous production of a carbon-mineralized slurry. The material storage and application module includes a material storage bin and a mixing machine connected with each other, and the material storage bin is configured to receive and store the carbon-mineralized slurry from a slurry bin. The mixing machine is configured to mix and prepare the carbon-mineralized slurry into a commodity concrete.

Abstract: The present invention is a rainfall prediction method, system, device and medium based on machine learning, which relates to the field of meteorological prediction technology. It uses atmospheric precipitable water volume (PWV) data, rainfall data and related meteorological parameters to input into a trained rainfall prediction network, and realizes accurate prediction of rainfall through an improved Transformer model. The model includes an encoder, a decoder and a final output layer, wherein feature extraction is performed inside the encoder through a multi-head probabilistic sparse self-attention module and a distillation module, and the encoder output containing feature information is used as the input of the decoder.

Abstract: A method for preparing a p-type gallium oxide film is provided. An MxGa1-xN target material is subjected to ablating, sputtering or evaporation in a vacuum chamber via physical vapor deposition to obtain MxGa1-xN clusters, where M is selected from the group consisting of Al, Sc, In, Y and Lu, and 0<x<1. The MxGa1-xN clusters are oxidized by O2 to obtain M-N co-doped p-type gallium oxide film on a substrate. The MxGa1-xN target material is prepared from MN powder and GaN powder through ball milling, pressing and sintering. A p-type gallium oxide film prepared by the method, and its application in the manufacturing of solar-blind ultraviolet detection devices and high-power electronic devices are also provided.

Abstract: Disclosed are a tungsten hexafluoride preparation method and apparatus based on photoelectric synergy. A photocatalyst and metal tungsten are sequentially filled in a discharge area of a plasma reactor in a direction of gas entry, and the discharge area of the plasma reactor is irradiated with light at the same time; the background gas generates a large amount of plasma in the discharge area, SF6 undergoes decomposition under the synergistic effect of photocatalysis and plasma, SF6 is decomposed to generate fluorine atoms and low-fluorine sulfides such as SF5 and SF4. The generated fluorine ions, SF5, SF4 and low-fluorine sulfides further react with metal tungsten to generate WF6 specialty gas, which not only realizes the utilization of fluorine resources of SF6, but also replaces highly toxic fluorine gas with non-toxic SF6 exhaust gas in the plasma reactor for reaction, thereby ensuring safe operation and low energy consumption.

Abstract: Disclosed are a tungsten hexafluoride preparation method and apparatus based on photoelectric synergy. A photocatalyst and metal tungsten are sequentially filled in a discharge area of a plasma reactor in a direction of gas entry, and the discharge area of the plasma reactor is irradiated with light at the same time; the background gas generates a large amount of plasma in the discharge area, SF6 undergoes decomposition under the synergistic effect of photocatalysis and plasma, SF6 is decomposed to generate fluorine atoms and low-fluorine sulfides such as SF5 and SF4. The generated fluorine ions, SF5, SF4 and low-fluorine sulfides further react with metal tungsten to generate WF6 specialty gas, which not only realizes the utilization of fluorine resources of SF6, but also replaces highly toxic fluorine gas with non-toxic SF6 exhaust gas in the plasma reactor for reaction, thereby ensuring safe operation and low energy consumption.

Abstract: The disclosure belongs to the field of visible light communication, and specifically discloses a stripe image processing method and apparatus for camera optical communication. The method includes: obtaining a stripe image data set, the stripe image data set including a stripe image sample and a stripe sequence label corresponding to the stripe image sample and a stripe-free image label; based on the stripe image sample and the stripe sequence label corresponding to the stripe image sample and the stripe-free image label, training a generative adversarial network to obtain an image reconstruction model and a stripe extraction model; among them, the image reconstruction model serves as a generator of the generative adversarial network, and the stripe extraction model serves as a discriminator of the generative adversarial network.

Abstract: The present invention provides a cationic ?-cyclodextrin and a preparation method and uses thereof, as well as a cationic ?-cyclodextrin functionalized silver nanoparticles and a preparation method and uses thereof. The cationic ?-cyclodextrin of the present invention is introduced with an amine group and quaternary ammonium groups, while retaining the special structure and properties of cyclodextrin itself. The amine group contained in the structure plays a role in reducing and complexing Ag+ in the synthesis of AgNPs, and plays a certain role in stabilizing nanoparticles and forming a complex in combination with quaternary ammonium groups.

Abstract: The disclosure provides an LED light identification method, device, electronic apparatus, and storage medium, belonging to the technical field of indoor positioning and navigation. The method includes: obtaining a background frame and a spline frame by a CMOS camera in an LED lighting environment; determining a target quantity of dark stripes in the spline frame by comparing the background frame with the spline frame; determining a target switching frequency corresponding to the target quantity based on a predetermined corresponding relationship and the target quantity. The target switching frequency is used to mark LED lights. By determining the target switching frequency corresponding to the target quantity and determining the target LED lights having the target switching frequency, it can be determined that the current position of the CMOS camera is near the target LED light.

Abstract: The disclosure provides an LED light source recognition method, device, apparatus, and medium based on deep learning, belonging to the field of indoor positioning and navigation technology. The method includes the following. In an LED lighting environment, a spline frame is obtained through a CMOS camera, in which the spline frame is an image having dark stripes. The spline frame is input to a target detection model, a dark stripe detection result output by the target detection model is obtained. The multiple rectangular boxes are preprocessed, and based on the predicted classification corresponding to each preprocessed rectangular box, an image feature encoding sequence of the spline frame is determined. The image feature encoding sequence is compared with a light source feature encoding sequence corresponding to each LED light source to determine the light source feature encoding sequence that best matches the spline frame.

Abstract: A method for producing sclareol by fermentation of cigar tobacco flower buds is provided, including: mixing cigar tobacco flower buds with deionized water, sterilizing and adding edible yeast, then fermenting at 25-30° C. and 100-300 rpm/min for 24-30 h. This method uses cigar tobacco flower buds as the sole raw material, without adding additional nutrients, and only ferments tobacco flower buds with edible yeast to synthesize sclareol. Meanwhile, this method has extremely high synthesis efficiency.

Abstract: Genetically modified Zymomonas mobilis to utilize inorganic N source, methods and uses thereof are provided. The genetically modified Z. mobilis has a genome with the knockout of a locus ZMO1107 from Z. mobilis ZM4 could ferment inorganic N source.

Abstract: Genetically modified Zymomonas mobilis to utilize inorganic N source, methods and uses thereof are provided. The genetically modified Z. mobilis has a genome with the knockout of a locus ZMO1107 from Z. mobilis ZM4 could ferment inorganic N source.

Abstract: A method and a device for preparing MoF6 based on plasma activation of SF6. The method includes the following steps: S1, filling a discharge area of a plasma reactor with molybdenum powder; S2, introducing an inert gas and an SF6 gas into the discharge area of the plasma reactor, where the inert gas is ionized into plasma, and the SF6 gas is ionized into fluorine atoms and low-fluorine sulfides after being activated by the plasma; and S3, generating an MoF6 gas and sulfur elements through reaction of the fluorine atoms and the low-fluorine sulfides with the molybdenum powder.

Abstract: Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof are provided. The recombinant Zymomonas mobilis carries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.

Abstract: The invention discloses a low power consumption and high precision resistance-free CMOS reference voltage source circuit, which includes a, a positive temperature coefficient voltage generation circuit and a starting circuit. The self-bias current source circuit uses two NMOS tubes with different threshold voltages in the subthreshold region to form a stack structure, which generates the bias current and negative temperature coefficient voltage on the order of nanoampere. The positive temperature coefficient voltage generation circuit uses PMOS differential to generate positive temperature coefficient voltage for the structure and performs first-order curvature compensation for negative temperature coefficient voltage.

Abstract: Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof are provided. The recombinant Zymomonas mobilis carries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.

Abstract: The present invention provides quaternary ammonium cyclodextrin and a preparation method and uses thereof, as well as a silver nanoparticle-cyclodextrin complex and a preparation method and uses thereof. The quaternary ammonium cyclodextrin of the present invention is introduced with an amine group and quaternary ammonium groups, while retaining the special structure and properties of cyclodextrin itself. The amine group contained in the structure plays a role in reducing and complexing Ag+ in the synthesis of AgNPs, and plays a certain role in stabilizing nanoparticles and forming a complex in combination with quaternary ammonium groups.

Abstract: The present invention discloses a method of preparing an alkali activation material by using red mud-based wet grinding and carbon sequestration and an application thereof. The preparation method includes: (1) adding water, red mud, a crystalline control agent, and a grinding aid into a wet grinding carbon sequestration apparatus to perform wet grinding, and simultaneously introducing CO2 until a slurry pH reaches 7 to 7.5; and removing wet grinding balls by a sieve to obtain a slurry A; (2) adding carbide slag, water and a water reducer to a wet planetary ball grinder tank for wet grinding, and removing wet grinding balls by a sieve to obtain a slurry B; (3) taking 50 to 80 parts of the slurry A and 20 to 50 parts of the slurry B and mixing them to obtain an alkali activation material.