Abstract: Application of glutamate dehydrogenase GdhA of Peptostreptococcus asaccharolyticus in increasing the yield of poly-?-glutamic acid from Bacillus licheniformis. The glutamate dehydrogenase GdhA of the Bacillus licheniformis WX-02 per se is replaced with the glutamate dehydrogenase derived from the Peptostreptococcus asaccharolyticus by means of homologous recombination, which significantly increases the level of synthesizing the poly-?-glutamic acid for the Bacillus licheniformis, and the yield of the obtained poly-?-glutamic acid from strains is increased at least by more than 20% compared with control strains.

Abstract: A genetically engineered strain of Z. mobilis and uses thereof are provided. The genetically engineered strain named ZMNP is obtained by knockouts of four endogenous plasmids of pZM32, pZM32, pZM36, pZM33, and pZM39 from strain ZM4-Cas12a. ZMNP contains a reduced genome and has a series of excellent traits, such as high transformation efficiency, enhanced tolerance to inhibitors, improved capability to use secondary mother liquor and other excellent performance. ZMNP can be used as a chassis cell to construct diverse cell factories for biochemical production using different feedstocks especially the lignocellulosic biomass.

Abstract: Disclosed are a device of complex gas mixture detection based on optical-path-adjustable spectrum detection and a method therefor, and the device includes: a light source configured for generating an incident beam and emitting the incident beam into an optical gas cell; the optical gas cell, including a cavity configured for accommodating a gas sample, and a reflection module group configured for reflecting the incident beam and a track arranged in the cavity, where the track is consistent with a light path of the light beam in the cavity; a detector module that is connected with the track in a relatively movable manner and is configured for receiving light beams and obtaining spectral data, where an optical path is changed by moving the detector module relative to the track; and a data acquisition unit that is configured for acquiring the spectral data obtained by the detector module.

Abstract: The present invention provides a method of preparing a recycled cementitious material by a phosphogypsum-assisted carbon sequestration pretreatment process. The method includes: (1) placing 100 mass parts of phosphogypsum, 1 to 2 mass parts of grinding aid, 10 to 20 mass parts of sodium-containing alkali component, 150 to 300 mass parts of zirconia balls, and 150 to 300 mass parts of water into a wet grinding tank for wet grinding. After 10 min to 30 min of wet grinding, introducing CO2 at a flow rate of 1.5 to 2.2 mass parts/min to keep a temperature of a wet grinding slurry below 40° C. When the wet grinding slurry reaches pH=10, ending the wet grinding and sieving out a wet grinding slurry; (2) mixing the wet grinding slurry with 700 to 1000 mass parts of slag and 100 to 350 mass parts of water to obtain a recycled cementitious material.

Abstract: A construction method of a regulation system for gene expression in Zymomonas mobilis and applications are provided. The T7 expression system in Zymomonas mobilis has been constructed. The T7 expression system is used to construct a regulation circuit, help analyze the function of toxic genes and realize the regulation of metabolic pathways in Zymomonas mobilis. At the same time, shuttle plasmids that can be used in Zymomonas mobilis and Escherichia coli, also be constructed to facilitate the construction of plasmid and improve the expression efficiency of foreign genes in Zymomonas mobilis, laying a foundation for subsequent protein expression and secretion and metabolic engineering pathway optimization regulation.

Abstract: The disclosure relates to a genetically engineered Z. mobilis ZM4 for producing PHB and uses thereof. A variety of strains capable of producing both PHB and ethanol that are constructed by integrating three codon-optimized essential genes for exogenous PUB biosynthesis, phaA, phaB and phaC, together with a tetracycline-inducible promoter Ptet into the genome of ZM4.

Abstract: The present invention provides a method of preparing a recycled cementitious material by a phosphogypsum-assisted carbon sequestration pretreatment process. The method includes: (1) placing 100 mass parts of phosphogypsum, 1 to 2 mass parts of grinding aid, 10 to 20 mass parts of sodium-containing alkali component, 150 to 300 mass parts of zirconia balls, and 150 to 300 mass parts of water into a wet grinding tank for wet grinding. After 10 min to 30 min of wet grinding, introducing CO2 at a flow rate of 1.5 to 2.2 mass parts/min to keep a temperature of a wet grinding slurry below 40° C. When the wet grinding slurry reaches pH=10, ending the wet grinding and sieving out a wet grinding slurry; (2) mixing the wet grinding slurry with 700 to 1000 mass parts of slag and 100 to 350 mass parts of water to obtain a recycled cementitious material.

Abstract: The present invention discloses a method of preparing an alkali activation material by using red mud-based wet grinding and carbon sequestration and an application thereof. The preparation method includes: (1) adding water, red mud, a crystalline control agent, and a grinding aid into a wet grinding carbon sequestration apparatus to perform wet grinding, and simultaneously introducing CO2 until a slurry pH reaches 7 to 7.5; and removing wet grinding balls by a sieve to obtain a slurry A; (2) adding carbide slag, water and a water reducer to a wet planetary ball grinder tank for wet grinding, and removing wet grinding balls by a sieve to obtain a slurry B; (3) taking 50 to 80 parts of the slurry A and 20 to 50 parts of the slurry B and mixing them to obtain an alkali activation material.

Abstract: The present invention provides a target tracking method, system, device and storage medium, which includes: Determining a target area based on the current frame of a training sample, extracting and fusing histogram of oriented gradient (HOG), color naming (CN), and color space HSV features of the target area to obtain a target template; Determining a target function according to the target template and a spatial regularization weight factor; Introducing the Sherman-Morrison formula into the alternating direction method of multipliers (ADMM) to accelerate the solution of the target function and obtain the response value; Iterating the target tracking model when the response value meets the preset confidence threshold until training is completed to obtain a trained target tracking model, and tracking the target in the video to be observed by using the trained target tracking model.

Abstract: The present disclosure belongs to the technical field of intrusion detection, specifically provides an intrusion detection method based on an improved immune network algorithm, and an application thereof The method includes: S1, initializing an antibody group; S2, calculating affinities between antigens and antibodies; S3, searching for a pair of inhomogeneous antigens which have the highest affinities and referred to as a duality antigen; S4, determining a boundary antibody set C; S5, determining a neighbor antibody set; S6, cloning and mutating, according to the affinities, obsolete antibodies to update a subnetwork to which the antigens belong; S7, calculating an average affinity between the antibodies in the boundary antibody set C and the antigens in the duality antigen; and S8, inhibiting the network, and simplifying the network to output a result network subset. The solution has relatively high detection accuracy and relatively low false alarm rate.

Abstract: A construction method of a tight regulation system for gene expression in Zywmmonas mobilis and applications are provided. The T7 expression system in Zymomonas mobilis has been constructed. The T7 expression system is used to construct a tight regulation circuit, help analyze the function of toxic genes and realize the tight regulation of metabolic pathways in Zymomonas mobilis. At the same time, a shuttle plasmids that can be used in Zymomonas mobilis and Escherichia coli., also be constructed to facilitate the construction of plasmid and improve the expression efficiency of foreign genes in Zymomonas mobilis, laying a foundation for subsequent protein expression and secretion and metabolic engineering pathway optimization regulation.

Abstract: Use of 2-pentanone and specific receptor thereof in a manufacture of a product regulating a cell function, a regulation of cell function, a manufacture of a product promoting an increase in an intracellular calcium ion concentration, or a manufacture of a product promoting an increase in a neuronal firing rate is provided. In the present disclosure, the specific receptor of 2-pentanone is expressed in cultured cells or animals, and its specific binding to 2-pentanone opens ligand-gated cation channels, resulting in an increase of intracellular calcium ion concentration, depolarization of cell membranes, and generation of electrical activity or endocrine activity, thereby finally achieving precise regulation of tissue cells and organ functions. After being treated, cells can be activated rapidly, producing effects with a rapid onset; once the treatment is stopped, the experimental effect can be quickly terminated to allow the cells to return to their original state quickly.

Abstract: A planetary gear train automatic limited slip differential may consist of a main differential, a planetary gear train differential controller, a left axle shaft, a right axle shaft, and a clutch. The planetary gear train differential controller may be composed of a first planetary gear train differential controller unit and a second planetary gear train differential controller unit. The first planetary gear train differential controller unit may be composed of a first planetary gear train and a first overrunning clutch connected to the first planetary gear train. The second planetary gear train differential controller unit may be composed of a second planetary gear train and a second overrunning clutch connected to the second planetary gear train.

Abstract: A method for controlling tobacco bacterial wilt and an application of NaHS in controlling the tobacco bacterial wilt are provided by the present disclosure, and the disclosure belongs to the technical field of plant disease control. By measuring the growth effect of Ralstonia solanacearum in tobacco through plate and shake bottle experiments, the disclosure finds the NaHS has an inhibiting effect on the growth of the pathogen of bacterial wilt; and the influence of different concentration of NaHS on the incidence of bacterial wilt in tobacco is detected, and the result also shows that NaHS has a good effect on resisting bacterial wilt in tobacco. The disclosure finds and proves for the first time that NaHS is an effective antibacterial substance against bacterial wilt and provides a new method for prevention and controlling of the tobacco bacterial wilt.

Abstract: Provided is a vehicle measurement system for an automatic conveyor. The measurement system includes: an information measurement unit, disposed at a position higher than the ready-for-loading vehicle and including two laser radars: a rear radar and a side radar; a voice prompt unit, configured to send a voice prompt; and a control unit; where when the ready-for-loading vehicle is approaching, the rear radar measures a distance between it and the ready-for-loading vehicle in real time as a predetermined parking distance; when a value of the predetermined parking distance is within a predetermined range, the control unit controls the voice prompt unit to send a voice prompt for parking; and after the ready-for-loading vehicle stops, the information measurement unit measures the distance between the ready-for-loading vehicle and the rear radar, carriage size information, and a deviation angle of a parking position of the ready-for-loading vehicle from the predetermined parking area.

Abstract: A planetary gear train automatic limited slip differential may consist of a main differential, a planetary gear train differential controller, a left axle shaft, a right axle shaft, and a clutch. The planetary gear train differential controller may be composed of a first planetary gear train differential controller unit and a second planetary gear train differential controller unit. The first planetary gear train differential controller unit may be composed of a first planetary gear train and a first overrunning clutch connected to the first planetary gear train. The second planetary gear train differential controller unit may be composed of a second planetary gear train and a second overrunning clutch connected to the second planetary gear train.

Abstract: The present disclosure belongs to the technical field of intrusion detection, specifically provides an intrusion detection method based on an improved immune network algorithm, and an application thereof The method includes: S1, initializing an antibody group; S2, calculating affinities between antigens and antibodies; S3, searching for a pair of inhomogeneous antigens which have the highest affinities and referred to as a duality antigen; S4, determining a boundary antibody set C; S5, determining a neighbor antibody set; S6, cloning and mutating, according to the affinities, obsolete antibodies to update a subnetwork to which the antigens belong; S7, calculating an average affinity between the antibodies in the boundary antibody set C and the antigens in the duality antigen; and S8, inhibiting the network, and simplifying the network to output a result network subset. The solution has relatively high detection accuracy and relatively low false alarm rate.

Abstract: A method for controlling tobacco bacterial wilt and an application of NaHS in controlling the tobacco bacterial wilt are provided by the present disclosure, and the disclosure belongs to the technical field of plant disease control. By measuring the growth effect of Ralstonia solanacearum in tobacco through plate and shake bottle experiments, the disclosure finds the NaHS has an inhibiting effect on the growth of the pathogen of bacterial wilt; and the influence of different concentration of NaHS on the incidence of bacterial wilt in tobacco is detected, and the result also shows that NaHS has a good effect on resisting bacterial wilt in tobacco. The disclosure finds and proves for the first time that NaHS is an effective antibacterial substance against bacterial wilt and provides a new method for prevention and controlling of the tobacco bacterial wilt.

Abstract: An apparatus and test method for simulating spark discharge of a high-voltage electrostatic precipitator are provided. The simulation apparatus includes a pulse power supply configured to provide a test voltage, an anode cylinder configured to simulate an anode of a precipitator, a cathode rod configured to simulate a cathode of the precipitator, a pulse capacitor unit configured to simulate an electrode capacitor of the precipitator, an insulating support configured to hang the cathode rod, a voltage divider configured to measure an electrode voltage, and a grounded current sampling unit configured to measure grounded current. The simulation apparatus simulates a discharge process of the high-voltage electrostatic precipitator to measure discharge characteristic parameters such as discharge current and discharge energy of the high-voltage electrostatic precipitator. In this way, characteristics of spark discharge of the precipitator under different load conditions are simulated.

Abstract: An apparatus and test method for simulating spark discharge of a high-voltage electrostatic precipitator are provided. The simulation apparatus includes a pulse power supply configured to provide a test voltage, an anode cylinder configured to simulate an anode of a precipitator, a cathode rod configured to simulate a cathode of the precipitator, a pulse capacitor unit configured to simulate an electrode capacitor of the precipitator, an insulating support configured to hang the cathode rod, a voltage divider configured to measure an electrode voltage, and a grounded current sampling unit configured to measure grounded current. The simulation apparatus simulates a discharge process of the high-voltage electrostatic precipitator to measure discharge characteristic parameters such as discharge current and discharge energy of the high-voltage electrostatic precipitator. In this way, characteristics of spark discharge of the precipitator under different load conditions are simulated.