Abstract: A method for filling a dispensing bag includes inserting a collapsed bag within the chamber of a bin, the chamber being bounded by a floor and an upstanding sidewall. A first port formed on the collapsed bag is securely fixed within a port hole formed on the floor of the bin. A hoist mounted on the bin is manipulated so that the hoist raises an upper end of the collapsed bag within the chamber while the first port remains fixed within the port hole so as to expand the collapsed bag within the chamber of the bin.

Abstract: A fluid bin assembly includes a bin having a floor with a side wall upstanding from the floor. The floor and the side wall bound a chamber. An opening extends through the floor and communicates with the chamber. One or more retention plates are removably mounted to the floor of the bin so as to at least partially cover the opening extending through the floor. The retention plates at least partially bound one or more portholes that openly communicate with the chamber of the bin.

Abstract: A sampling port includes an elongated flexible support tube having an interior surface bounding a first passageway extending between a first end and an opposing second end, the first passageway being closed at the first end of the support tube and open at the second end of the support tube. An elongated flexible sampling tube has an interior surface bounding a second passageway extending between a first end and an opposing second end, the second passageway being open at the first end of the sampling tube, at least a portion of the sampling tube adjacently extending along the length of the support tube and being connected to the support tube. A flange encircles and radially outwardly projecting from the support tube and the sampling tube at a location spaced apart from the first end of the support tube and the first end of the sampling tube.

Abstract: A sampling port includes an elongated flexible support tube having an interior surface bounding a first passageway extending between a first end and an opposing second end, the first passageway being closed at the first end of the support tube and open at the second end of the support tube. An elongated flexible sampling tube has an interior surface bounding a second passageway extending between a first end and an opposing second end, the second passageway being open at the first end of the sampling tube, at least a portion of the sampling tube adjacently extending along the length of the support tube and being connected to the support tube. A flange encircles and radially outwardly projecting from the support tube and the sampling tube at a location spaced apart from the first end of the support tube and the first end of the sampling tube.

Abstract: A system for forming a fluid connection includes a first connector and a second connector. Both connectors include a tubular body having a membrane mounted on a distal end thereof. A support member facilitates the coupling of the connectors together so that the membranes are abutted together. Radiant energy is applied to the abutted membranes so as to first sterilize the membranes and then melt the membranes so that a passage is formed therethrough.

Abstract: A probe assembly includes a tubular sleeve having a passage extending between a first end and an opposing second end. The tubular sleeve is movable between an extended position wherein the first end and the opposing second end are spaced apart and a collapsed position wherein the first end and the opposing second end are moved closer together. A connector is secured to the second end of the tubular sleeve, the connector having an opening extending therethrough that communicates with the passage of the tubular sleeve, a sealing layer removably covering the opening of the connector. An elongated probe has a first end and an opposing second end, the second end of the probe being positioned within the passage of the tubular sleeve, the second end of the probe being configured to pass through the opening of the connector when the sealing layer is removed therefrom.

Abstract: A mobile first housing bounds a substantially sterile clean room, a filtration room, and at least one change room communicating between clean room. A fluid filtration system is disposed within the first housing, the filtration system includes a first support container in which a disposable fill bag is disposed. A disposable fluid line extends between the fill bag and the at least one filter. A support bin is also disposed within the first housing. A disposable pooling bag is disposed within the support bin, the pooling bag being in fluid communication with the at least one filter. A disposable fill line has a first end in fluid communication with the pooling bag and an opposing second end disposed within the clean room.

Abstract: A fluid bin assembly includes a bin having a floor with a side wall upstanding therefrom. The floor and the side wall bound a chamber. The floor has an opening extending therethrough that communicates with the chamber. A retention plate is removably mounted to the floor of the bin so as to at least partially cover the opening extending therethrough. The retention plate at least partially bounds a porthole that openly communicates with the chamber of the bin.

Abstract: A fluid bin assembly includes a bin having a floor with a side wall upstanding therefrom. The floor and the side wall bound a chamber. The floor has an opening extending therethrough that communicates with the chamber. A retention plate is removably mounted to the floor of the bin so as to at least partially cover the opening extending therethrough. The retention plate at least partially bounds a porthole that openly communicates with the chamber of the bin.

Abstract: A container system includes a substantially rigid container having a floor and a side wall upstanding therefrom. The side wall and floor bound a chamber. A magnetic mixer is disposed below the floor of the container. A mixing bag assembly is disposed within the chamber of the container. The mixing bag assembly includes a collapsible body having a first end and an opposing second end. The collapsible body bounds a compartment. A mixing dish is disposed at the second end of the collapsible body so as to communicate with the compartment thereof. A magnetic stir bar is disposed on the mixing dish.

Abstract: A mobile first housing bounds a substantially sterile clean room, a filtration room, and at least one change room communicating between clean room. A fluid filtration system is disposed within the first housing, the filtration system includes a first support container in which a disposable fill bag is disposed. A disposable fluid line extends between the fill bag and the at least one filter. A support bin is also disposed within the first housing. A disposable pooling bag is disposed within the support bin, the pooling bag being in fluid communication with the at least one filter. A disposable fill line has a first end in fluid communication with the pooling bag and an opposing second end disposed within the clean room.

Abstract: A container assembly includes a container bounding a chamber and having a floor. A well is mounted on the floor of the container. The well bounds a compartment that extends below the floor of the container and communicates with the chamber of the container. An anchor has a side wall with a first port opening formed at a first end and a second port opening formed on the side wall or at a second end of the anchor. A fluid passageway extends between the first port opening and the second port opening. At least a portion of the anchor is secured within the compartment of the well such that at least a portion of the second port opening is disposed within the compartment. A dip tube is fluid coupled with the first port opening of the anchor.

Abstract: A container system includes a substantially rigid container having a floor and a side wall upstanding therefrom. The side wall and floor bound a chamber. A magnetic mixer is disposed below the floor of the container. A mixing bag assembly is disposed within the chamber of the container. The mixing bag assembly includes a collapsible body having a first end and an opposing second end. The collapsible body bounds a compartment. A mixing dish is disposed at the second end of the collapsible body so as to communicate with the compartment thereof. A magnetic stir bar is disposed on the mixing dish.

Abstract: A mixing system includes a housing and a motor mount disposed on the housing and having a passage extending therethrough. A dive motor is coupled with the motor mount for selectively rotating the motor mount relative to the housing. A rotational assembly includes a hub having a passageway extending therethrough and a casing at least partially encircling the hub, the hub being rotatable to the casing. The rotational assembly is removably coupled to the housing so that the passageway of the hub aligns with the passage of the motor mount.

Abstract: A method for extracting blood from animal fetus includes securing a placenta of an animal against a substantially planar face, the first face being disposed at an angle in a range between about 45° to about 110° relative to the horizontal. The placenta is massaged against the first face so that at least a portion of the blood within the placenta flows out of the placenta through an umbilical cord and into the animal fetus. The animal fetus is then suspended. At least a portion of the blood within the animal fetus is then drained from the fetus into a container.

Abstract: The present invention relates to a stirred-tank reactor system and methods of preparing such systems. The present invention further encompasses the use of the stirred-tank reactor system as a disposable bioreactor and in kits with disposable elements.

Abstract: A container system includes a substantially rigid container having a floor and a side wall upstanding therefrom. The side wall and floor bound a chamber. A magnetic mixer is disposed below the floor of the container. A mixing bag assembly is disposed within the chamber of the container. The mixing bag assembly includes a collapsible body having a first end and an opposing second end. The collapsible body bounds a compartment. A mixing dish is disposed at the second end of the collapsible body so as to communicate with the compartment thereof. A magnetic stir bar is disposed on the mixing dish.

Abstract: A method for forming a sterile fluid connection includes inserting a dispensing end of a fill tube into a cavity of a sterilizer. A sterilizing field is generated within the cavity of the sterilizer. A cap mounted on the end of the fill tube is removed from the fill tube while in the cavity. A fill port is disposed within the cavity. The fill tube is fluid coupled with the fill port in the cavity of the sterilizer while the dispensing end of the fill tube and the fill port are exposed to the sterilizing field. A fluid is passed from the fill tube to the fill port.

Abstract: A fluid delivery system includes a flexible media bag having an outlet spout projecting therefrom. A passageway longitudinally extends through the outlet spout and communicates with the interior of the media bag. A diptube connector is mounted to the outlet spout. The diptube connector includes a stem having a first end with a barb radially projecting out therefrom and an opposing second end with an annular flange radially projecting out therefrom. The flange has an outer diameter greater than the inner diameter of the passageway in the outlet spout. The first end of the diptube connector is received in sealed engagement within the first end of the diptube. The second end of the diptube is passed through the passageway in the outlet spout such that the flange on the diptube connector rests on the outlet spout. The outlet spout having the diptube connector mounted thereon is received in sealed engagement within a delivery tube.

Abstract: The present invention provides media and methods for the maintenance or promotion of growth of a cellular line, particularly a hybridoma cellular line. A serum blend is disclosed comprising fetal bovine serum and agamma bovine calf serum. The serum blend is added to other types of support media and can be placed in association with a hybridoma cellular line. The cellular media are preferably employed with hybridoma cellular lines in an environment having a carbon dioxide atmosphere in the range from about ambient levels to 10%, and at a temperature in the range from about 32.degree. C. to 40.degree. C.In addition, the present invention provides for cellular lines maintained and grown in association with such a serum blend. Also, a serum used in a medium for producing diagnostic and therapeutic biologicals from cellular lines, particularly, hybridoma cellular lines, comprising a serum blend of fetal bovine serum and agamma bovine calf serum is provided.