Patents Assigned to Immunex
  • Human serpin polypeptides
    Patent number: 7132264
    Abstract: This invention relates to Thypin, a new member of the human serpin polypeptide family, methods of making Thypin polypeptides and using these polypeptides to treat various medical disorders and to methods of screening for compounds that agonize or antagonize Thypin polypeptide activities.
    Type: Grant
    Filed: September 2, 2005
    Date of Patent: November 7, 2006
    Assignee: Immunex Corporation
    Inventors: Howard R. G. Clarke, Robert F. DuBose, Steven R. Wiley
  • Site specific protein modification
    Patent number: 7129203
    Abstract: Processes for conjugating proteins with polyethylene glycol are disclosed. The disclosed processes provide modified proteins having little or no decrease in their activity and include the steps of deleting at least one amino acid residue on the protein, replacing the at least one amino acid residue with an amino acid residue that does not react with polyethylene glycol, and contacting the protein with polyethylene glycol under conditions sufficient to conjugate the polyethylene glycol to the protein. This advantageous retention of a desired protein activity is attributed to the availability of one or more protein binding sites which is unaltered in the conjugation process and thus remains free to interact with a binding partner ligand or cognate subsequent to the conjugation process.
    Type: Grant
    Filed: September 12, 2002
    Date of Patent: October 31, 2006
    Assignee: Immunex Corporation
    Inventor: Dean K. Pettit
  • Methods for treating autoimmune and chronic inflammatory conditions using antagonists of CD30 or CD30L
    Patent number: 7122183
    Abstract: The invention provides methods of treating autoimmune and chronic inflammatory conditions by administering agents that hinder the CD30/CD30L interaction, combination treatments, and methods of treating conditions resistant to treatment with TNF? inhibitors by administering agents that inhibit signal transduction by CD30 or IL-1. Included also are treatments involving concurrently administering agents that block the CD30/CD30L interaction and agents that antagonize the IL-4/IL-4R interaction. Additionally provided is an animal model for screening candidate agents for their efficacy in treating conditions that are resistant to treatment with TNF? inhibitors.
    Type: Grant
    Filed: August 26, 2003
    Date of Patent: October 17, 2006
    Assignee: Immunex Corporation
    Inventors: Kendall M. Mohler, Dauphine S. Barone, Jacques J. Peschon, Mary K. Kennedy, John D. Pluenneke
  • Methods for purifying protein
    Patent number: 7122641
    Abstract: A method is provided for separating a protein from one or more other proteins using hydroxyapatite chromatography in which the protein does not bind to hydroxyapatite but the other protein(s) does. In some embodiments, a second protein affixed to a solid support has been used previously to purify the protein by affinity chromatography, and small amounts of the second protein are introduced in the sample during this process. The protein being purified can comprise at least one constant antibody immunoglobulin domain. The second protein can bind to proteins comprising such a domain.
    Type: Grant
    Filed: December 20, 2002
    Date of Patent: October 17, 2006
    Assignee: Immunex Corporation
    Inventors: Ganesh Vedantham, Clayton A. Brooks, III, Joanne M. Reeder, Andrew M. Goetze
  • Human TSLP polynucleotides
    Publication number: 20060223754
    Abstract: The invention is directed to purified and isolated novel TSLP polypeptides, the nucleic acids encoding such polypeptides, processes for production of recombinant forms of such polypeptides, antibodies generated against these polypeptides, fragmented peptides derived from these polypeptides, and the uses of the above.
    Type: Application
    Filed: June 13, 2006
    Publication date: October 5, 2006
    Applicant: Immunex Corporation
    Inventors: John Sims, Stewart Lyman, Hilary McKenna, Allison Armstrong
  • Control of ph transitions during chromatography
    Patent number: 7101982
    Abstract: The invention provides methods of controlling pH variations during chromatography. In particular embodiments, the invention includes equilibrating and eluting proteins from chromatography resins have a solid phase that possesses a charged functional group on its backbone under controlled conditions of osmolarity and pH. The invention is particularly useful in the purification of proteins that are sensitive to pH transitions such as, for example, the IL-1 Receptor type II protein.
    Type: Grant
    Filed: March 29, 2002
    Date of Patent: September 5, 2006
    Assignee: Immunex Corporation
    Inventors: Sanchayita Ghose, Thomas M. McNerney
  • Regulation of translation for recombinant protein production
    Patent number: 7091004
    Abstract: The invention provides cells that produce increased levels of recombinant protein by modulating the activity of translational regulator gene products that are downstream targets of PKB alpha, methods of making such cells, and methods of using such cells. Such translational regulator gene products include 4E-BP1 and mTOR.
    Type: Grant
    Filed: June 7, 2002
    Date of Patent: August 15, 2006
    Assignee: Immunex Corporation
    Inventors: Arvia E. Morris, Sharon T. Wong-Madden
  • Integrin antagonists
    Publication number: 20060177443
    Abstract: The present invention provides methods and compositions for inhibiting the biological activity of integrins, for inhibiting endothelial cell migration, and for inhibiting angiogenesis. In particular, the invention provides compositions comprising ADAM disintegrin domains and methods for using said compositions. In preferred embodiments the methods and compositions of the invention are used to inhibit angiogenesis and to treat diseases or conditions mediated by angiogenesis.
    Type: Application
    Filed: March 28, 2006
    Publication date: August 10, 2006
    Applicant: Immunex Corporation
    Inventors: William Fanslow, Douglas Cerretti, Kurt Poindexter, Roy Black
  • Polypeptide purification reagents and methods for their use
    Patent number: 7083948
    Abstract: The present invention provides polypeptide purification reagents that are, themselves, proteins and methods for using these to purify almost any protein of interest. A polypeptide purification reagent comprises a polypeptide domain that can bind to a protein of interest and a polypeptide domain that confers a propensity to precipitate under conditions where the vast majority of proteins will not precipitate. In addition or as an alternative, a polypeptide purification reagent can comprise amino acid sequences that confer an ability to partition into a phase in a liquid—liquid phase system that the majority of proteins do not partition into. Methods for purifying a protein of interest using such polypeptide purification reagents are also provided.
    Type: Grant
    Filed: December 19, 2003
    Date of Patent: August 1, 2006
    Assignee: Immunex Corporation
    Inventors: Helmut M. Sassenfeld, Rebecca E. McCoy
  • IL-1 receptor accessory protein
    Publication number: 20060166256
    Abstract: DNA encoding IL-1R AcP splice variant polypeptides, the polypeptides and methods for using the encoded polypeptides are disclosed.
    Type: Application
    Filed: March 23, 2006
    Publication date: July 27, 2006
    Applicant: IMMUNEX CORPORATION
    Inventors: John Sims, Dirk Smith
  • Human B7 polypeptide B7-H3A
    Publication number: 20060154313
    Abstract: This invention relates to B7-H3A, a new member of the human B7 polypeptide family, methods of making such polypeptides, and to methods of using them to treat immunological conditions and to identify compounds that alter B7-H3A polypeptide activities.
    Type: Application
    Filed: March 17, 2006
    Publication date: July 13, 2006
    Applicant: Immunex Corporation
    Inventors: Dirk Anderson, John Marken
  • Use of integrin antagonists to inhibit angiogenesis
    Patent number: 7074408
    Abstract: The present invention provides methods and compositions for inhibiting the biological activity of integrins, for inhibiting endothelial cell migration. and for inhibiting angiogenesis. In particular, the invention provides compositions comprising ADAM disintegrin domains and methods for using said compositions. In preferred embodiments the methods and compositions of the invention are used to inhibit angiogenesis and to treat diseases or conditions mediated by angiogenesis.
    Type: Grant
    Filed: February 23, 2001
    Date of Patent: July 11, 2006
    Assignee: Immunex Corporation
    Inventors: William C. Fanslow, III, Douglas P. Cerretti, Kurt M. Poindexter, Roy A. Black
  • Tek antagonists
    Patent number: 7067475
    Abstract: The present invention provides Tek antagonists and methods of inhibiting angiogenesis in a mammal by administering Tek antagonists. The methods are particularly useful in treating diseases or conditions mediated by angiogenesis, such as solid tumors and diseases or conditions characterized by ocular neovascularization.
    Type: Grant
    Filed: February 3, 2003
    Date of Patent: June 27, 2006
    Assignee: Immunex Corporation
    Inventors: Douglas P. Cerretti, Luis G. Borges, William C. Fanslow, III
  • Methods of using mutant flt3-ligand polypeptides to induce cellular expansion
    Patent number: 7067118
    Abstract: A screening method for identifying mutant polypeptides having at least one amino acid difference from a wild type protein involved in a receptor-ligand interaction is disclosed. Also disclosed are mutant polypeptides of the hematopoietic growth factor flt3-Ligand (flt3-L) identified using this method, nucleic acids encoding these flt3-L mutant polypeptides, and methods of treatment involving in vitro and in vivo use of the mutant polypeptides and nucleic acids.
    Type: Grant
    Filed: July 16, 2001
    Date of Patent: June 27, 2006
    Assignee: Immunex Corporation
    Inventors: Thomas J. Graddis, Jeffrey T. McGrew
  • Cell culture performance with betaine
    Patent number: 7067279
    Abstract: This invention relates generally to the field of cell culture. More particularly, the invention relates to improving viability of recombinant cell cultures and the yields of secreted polypeptides therefrom by the addition of betaine to the tissue culture medium.
    Type: Grant
    Filed: August 23, 2002
    Date of Patent: June 27, 2006
    Assignee: Immunex Corporation
    Inventors: Brian D. Follstad, Anne H. Potter
  • Antibodies which specifically bind to TNF-R
    Patent number: 7057022
    Abstract: Tumor necrosis factor receptor proteins, DNAs and expression vectors encoding TNF receptors, and processes for producing TNF receptors as products of recombinant cell culture, are disclosed.
    Type: Grant
    Filed: April 23, 2003
    Date of Patent: June 6, 2006
    Assignee: Immunex Corporation
    Inventors: Craig A. Smith, Raymond G. Goodwin, M. Patricia Beckmann
  • Polypeptide analyses using stable isotope labeling
    Patent number: 7052916
    Abstract: Methods for incorporating a stable isotope into a protein or peptide fragment are disclosed. The methods include providing isolated isotope-labeled protein or peptide fragments and analyzing the isolated labeld fragments. In another aspect of the invention, methods for quantitatively comparing peptides in two protein or peptide fragment mixtures are provided. In these methods, protein levels are measured using stable-isotope coded protein or peptide fragments.
    Type: Grant
    Filed: May 24, 2002
    Date of Patent: May 30, 2006
    Assignee: Immunex Corporation
    Inventor: Richard S. Johnson
  • Human and murine cytokine polypeptides
    Publication number: 20060110772
    Abstract: This invention relates to IMX7189 cytokines and to new members of the human cytokine polypeptide family, methods of making such polypeptides, and to methods of using them to treat conditions and diseases involving proliferation and/or differentiation of cells from pluripotent stem cell precursors, and to identify compounds that alter cytokine polypeptide activities.
    Type: Application
    Filed: July 7, 2005
    Publication date: May 25, 2006
    Applicant: Immunex Corporation
    Inventors: Peter Baum, Bruce Mosley, Randal Ketchem
  • DNA encoding IL-1 receptor accessory protein
    Patent number: 7049095
    Abstract: DNA encoding IL-1R AcP splice variant polypeptides, the polypeptides and methods for using the encoded polypeptides are disclosed.
    Type: Grant
    Filed: October 26, 2001
    Date of Patent: May 23, 2006
    Assignee: Immunex Corporation
    Inventors: John E. Sims, Dirk E. Smith
  • Antibodies against flt3-ligand
    Patent number: 7045128
    Abstract: Ligands for flt3 receptors capable of transducing self-renewal signals to regulate the growth, proliferation or differentiation of progenitor cells and stem cells are disclosed. The invention is directed to anti-flt3-L antibodies and enzyme-linked immunosorbent assays comprising such antibodies.
    Type: Grant
    Filed: March 13, 2002
    Date of Patent: May 16, 2006
    Assignee: Immunex Corporation
    Inventors: Stewart D. Lyman, M. Patricia Beckmann