Abstract: The invention encompasses methods for increasing genetic progress in livestock, and for genetic dissemination, including the use of amniocentesis to obtain fetal amniocytes for use in genomic evaluation and cloning.
Type:
Application
Filed:
April 13, 2017
Publication date:
September 28, 2017
Applicant:
Inguran, LLC
Inventors:
Juan F. Moreno, John Dobrinsky, David Kendall, Claas Heuer
Abstract: This disclosure relates to a system for sorting sperm cells in a microfluidic chip. In particular, various features are incorporated into the system for aligning and orienting sperm in flow channels, as well as, for determining sperm orientation and measuring relative DNA content for analysis and/or sorting.
Type:
Grant
Filed:
March 14, 2013
Date of Patent:
September 12, 2017
Assignee:
Inguran, LLC
Inventors:
Johnathan Charles Sharpe, Nemanya Sedoglavich, Kris Buchanan, Blair Morad, Donald Francis Perrault, Jr., Erin Koksal
Abstract: The invention relates to methods of increasing the genetic progress of a line, breed or herd of swine through the use of sex-selected sperm cells in artificial insemination techniques. The invention also encompasses methods of artificially inseminating a swine via deep intrauterine catheter or via a laparoscopic procedure, which allow the use of reduced doses of sex-selected sperm cells.
Type:
Grant
Filed:
June 6, 2013
Date of Patent:
August 29, 2017
Assignee:
Inguran, LLC
Inventors:
Juan Moreno, Gregg Bevier, John Dobrinsky
Abstract: The invention encompasses methods for increasing genetic progress in livestock, and for genetic dissemination, including the use of amniocentesis to obtain fetal amniocytes for use in genomic evaluation and cloning.
Type:
Application
Filed:
October 14, 2016
Publication date:
April 20, 2017
Applicant:
Inguran, LLC
Inventors:
Juan F. Moreno, John Dobrinsky, David Kendall, Claas Heuer
Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.
Type:
Grant
Filed:
September 28, 2012
Date of Patent:
February 28, 2017
Assignee:
Inguran, LLC
Inventors:
Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
Abstract: A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.
Type:
Application
Filed:
June 10, 2016
Publication date:
November 10, 2016
Applicant:
Inguran, LLC
Inventors:
Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
Abstract: A nozzle assembly is disclosed. The nozzle assembly may include a nozzle outlet and enclose a nozzle cavity. A sample inlet formed in the nozzle housing may provide fluid communication to an injection tube mounted in an injection stem formed in the nozzle housing. One or more sheath inlet may provide fluid communication with the nozzle cavity and a downstream exit orifice.
Type:
Application
Filed:
July 7, 2016
Publication date:
November 3, 2016
Applicant:
Inguran, LLC
Inventors:
Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
Abstract: The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.
Type:
Application
Filed:
May 17, 2016
Publication date:
September 8, 2016
Applicant:
Inguran, LLC
Inventors:
Richard Lenz, Juan Moreno, Ramakrishnan Vishwanath
Abstract: The invention relates to methods of increasing the genetic progress of a line, breed or herd of swine through the use of sex-selected sperm cells in artificial insemination techniques. The invention also encompasses methods of artificially inseminating a swine via deep intrauterine catheter or via a laparoscopic procedure, which allow the use of reduced doses of sex-selected sperm cells.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
September 6, 2016
Assignee:
Inguran, LLC
Inventors:
Juan Moreno, Gregg BeVier, John Dobrinsky
Abstract: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.
Type:
Grant
Filed:
March 14, 2013
Date of Patent:
August 2, 2016
Assignee:
Inguran, LLC
Inventors:
Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
Abstract: A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.
Type:
Grant
Filed:
April 10, 2015
Date of Patent:
June 28, 2016
Assignee:
Inguran, LLC
Inventors:
Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
Abstract: Generally, polymeric members and laser marking methods for producing visible marks on polymeric members, such as on thin and/or curved surfaces. The laser marking methods can include methods of laser marking straws with the step of matching laser source properties to the properties of straws being marked or with the step of laser marking straws having photochromic dyes.
Type:
Grant
Filed:
April 17, 2012
Date of Patent:
June 7, 2016
Assignee:
Inguran, LLC
Inventors:
Johnathan Charles Sharpe, Thomas B. Gilligan, Richard W. Lenz, Juan F. Moreno
Abstract: A container suitable for storing biological material which is laser etched with a two-dimensional bar code and methods for producing the same. The container can be in the form of a straw having a thickness between about 0.1 mm and about 0.3 mm or can be in the form of another container that holds multiple straws. The laser etched mark can be in the form of a two-dimensional bar code may be located on an exterior surface of the container, and when the container is a straw, it remains unwarped and impermeable to fluids.
Type:
Grant
Filed:
October 18, 2013
Date of Patent:
June 7, 2016
Assignee:
Inguran, LLC
Inventors:
Thomas B. Gilligan, Sam Hogue, Juan Moreno, Johnathan Charles Sharpe
Abstract: The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.
Type:
Application
Filed:
November 20, 2015
Publication date:
May 26, 2016
Applicant:
INGURAN, LLC
Inventors:
Thomas B. Gilligan, Ramakrishnan Vishwanath, Kilby Willenburg
Abstract: Generally, compositions and methods for handling processed sperm populations including samples that are freshly collected, transported as fresh samples, as well as samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the sperm cell. Such trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the sperm's ability to fertilize an egg, grow into a healthy embryo and produce a healthy offspring. The novel compounds described can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
May 24, 2016
Assignee:
Inguran, LLC
Inventors:
Richard Lenz, Juan Moreno, Ramakrishnan Vishwanath
Abstract: The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
May 24, 2016
Assignee:
Inguran, LLC
Inventors:
Richard Lenz, Juan Moreno, Ramakrishnan Vishwanath
Abstract: The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.
Type:
Grant
Filed:
June 1, 2012
Date of Patent:
May 24, 2016
Assignee:
Inguran, LLC
Inventors:
Richard Lenz, Juan Moreno, Ramakrishnan Vishwanath
Abstract: The present invention generally relates to compositions and methods for the handling of processed sperm populations including samples that are freshly collected, those transported as fresh samples, as well as samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the sperm cell. Such trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the sperm's ability to fertilize an egg, grow into a health embryo and produce a healthy offspring.
Type:
Application
Filed:
January 18, 2016
Publication date:
May 12, 2016
Applicant:
Inguran, LLC
Inventors:
Richard Lenz, Juan Moreno, Ramakrishnan Vishwanath
Abstract: The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.