Patents Assigned to Inguran, LLC
  • Publication number: 20160010057
    Abstract: Embodiments of the claimed invention relate to a method of producing sex sorted sperm having an improved dose response which includes the steps of extending a sperm sample with a buffering holding media and adjusting the concentration of the extended sperm sample to a target concentration range. The extended sperm may then be stained with a DNA selective dye and sex sorted with a flow cytometer into a catch fluid. During the extending and sorting of the sperm sample, the pH may be maintained at a target pH. Additionally, at least one of the buffering holding media, the DNA selective dye and the catch fluid may be supplemented with at least one antioxidant.
    Type: Application
    Filed: September 22, 2015
    Publication date: January 14, 2016
    Applicant: INGURAN, LLC
    Inventors: Ramakrishnan Vishwanath, Kenneth Michael Evans, Thomas B. Gilligan, Juan Moreno, Richard Lenz, Clara Gonzalez-Marin
  • Patent number: 9222872
    Abstract: A nozzle tip formed from a cylindrical body defining a longitudinal axis and a frustoconical body adjoining the cylindrical body on the longitudinal axis. The cylindrical body may be in fluid communication with the frustoconical body. The frustoconical body may end in a flat surface with a nozzle exit orifice which is transverse to the longitudinal axis. There may be a cutout at the edge of the frustoconical body and the flat surface. The flow cytometer system may also include a source of electromagnetic radiation for producing a beam incident upon the fluid stream and the particles and a detector for detecting light emitted or reflected from the particles within the fluid stream in response to the beam.
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: December 29, 2015
    Assignee: Inguran, LLC
    Inventors: Kris Buchanan, Kenneth Michael Evans
  • Patent number: 9140688
    Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: September 22, 2015
    Assignee: Inguran, LLC
    Inventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
  • Publication number: 20150219546
    Abstract: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.
    Type: Application
    Filed: March 4, 2013
    Publication date: August 6, 2015
    Applicant: Inguran, LLC
    Inventors: Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
  • Publication number: 20150211978
    Abstract: A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.
    Type: Application
    Filed: April 10, 2015
    Publication date: July 30, 2015
    Applicant: Inguran, LLC
    Inventors: Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
  • Patent number: 9040304
    Abstract: A multi-channel system and methods for sorting particles according to one or more characteristics of the particles. The system includes multiple flow cytometry units, each unit can have a nozzle for producing a fluid stream containing a desired population of particles in a mixture of particles. Each of the units may be operable to sort said desired population of particles by interrogating the fluid stream with a beam of electromagnetic radiation and classifying particles based on one or more characteristics of the particles. The system also includes a common fluid delivery system for delivering sheath fluid to each flow cytometer unit for producing respective fluid streams.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: May 26, 2015
    Assignee: Inguran, LLC
    Inventors: Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
  • Patent number: 9027850
    Abstract: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: May 12, 2015
    Assignee: Inguran, LLC
    Inventors: Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
  • Publication number: 20150072340
    Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.
    Type: Application
    Filed: September 28, 2012
    Publication date: March 12, 2015
    Applicant: INGURAN, LLC
    Inventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
  • Patent number: 8865379
    Abstract: Generally, polymeric members and laser marking methods for producing visible marks on polymeric members, such as on thin and/or curved surfaces. The laser marking methods can include methods of laser marking straws with the step of matching laser source properties to the properties of straws being marked or with the step of laser marking straws having photochromic dyes.
    Type: Grant
    Filed: April 17, 2012
    Date of Patent: October 21, 2014
    Assignee: Inguran, LLC
    Inventors: Johnathan Charles Sharpe, Thomas B. Gilligan, Richard W. Lenz, Juan Moreno
  • Publication number: 20140273179
    Abstract: This disclosure relates to a device in the form of a microfluidic chip. In particular, various features are incorporated into the microfluidic chip for aligning and orienting sperm in flow channels, as well as for separating selected subpopulations of sperm.
    Type: Application
    Filed: March 14, 2013
    Publication date: September 18, 2014
    Applicant: INGURAN, LLC
    Inventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Erin Koksal, Kris Buchanan, Blair Morad
  • Publication number: 20140273192
    Abstract: This disclosure relates to a system for sorting sperm cells in a microfluidic chip. In particular, various features are incorporated into the system for aligning and orienting sperm in flow channels, as well as, for determining sperm orientation and measuring relative DNA content for analysis and/or sorting.
    Type: Application
    Filed: March 14, 2013
    Publication date: September 18, 2014
    Applicant: INGURAN, LLC
    Inventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Kris Buchanan, Blair Morad, Donald Francis Perrault, JR., Erin Koksal
  • Publication number: 20140273059
    Abstract: This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.
    Type: Application
    Filed: March 14, 2013
    Publication date: September 18, 2014
    Applicant: INGURAN, LLC
    Inventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Blair Morad, Donald Francis Perrault, JR.
  • Publication number: 20140234833
    Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 21, 2014
    Applicant: INGURAN, LLC
    Inventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
  • Publication number: 20140220620
    Abstract: A multi-channel system and methods for sorting particles according to one or more characteristics of the particles. The system includes multiple flow cytometry units, each unit can have a nozzle for producing a fluid stream containing a desired population of particles in a mixture of particles. Each of the units may be operable to sort said desired population of particles by interrogating the fluid stream with a beam of electromagnetic radiation and classifying particles based on one or more characteristics of the particles. The system also includes a common fluid delivery system for delivering sheath fluid to each flow cytometer unit for producing respective fluid streams.
    Type: Application
    Filed: March 12, 2014
    Publication date: August 7, 2014
    Applicant: INGURAN, LLC
    Inventors: Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
  • Publication number: 20140206930
    Abstract: This disclosure relates to methods of producing multiple births of the same sex. Some embodiments relate to methods of boosting the population growth of a herd with genetically desirable females by transferring two embryos fertilized with sex-sorted sperm to recipients.
    Type: Application
    Filed: March 14, 2013
    Publication date: July 24, 2014
    Applicant: INGURAN, LLC
    Inventor: Juan Moreno
  • Publication number: 20140170696
    Abstract: Generally, a computer implemented remote monitoring system which generates a viewable reduced byte data representation for each one of a plurality of analyzed instrument signals. Specifically, a flow cytometer remote monitoring system which generates a viewable reduced byte data representation for each one of a plurality analyzed flow cytometer signals.
    Type: Application
    Filed: February 10, 2014
    Publication date: June 19, 2014
    Applicant: INGURAN, LLC
    Inventors: James J. Salinas, Kenneth M. Evans
  • Patent number: 8748183
    Abstract: A method of continuously verifying proper sort calibration in a droplet sorting flow cytometer by selecting a fraction of droplets estimated to have substantially zero probability of containing a particle; applying one charge of a set of charges to the selected droplets in order to form a test stream out of the selected droplets; illuminating the droplets in the test stream; and detecting any light emitted or scattered by any particles in the selected droplets.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: June 10, 2014
    Assignee: Inguran, LLC
    Inventors: Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
  • Publication number: 20140142421
    Abstract: The invention relates to methods of increasing genetic merit of swine by establishing a plurality of mating subtypes for a line of swine, and determining a percentage of progeny that are male for each of the mating subtypes, or a percentage of progeny that are female for each of the mating subtypes, that would result, relative to a control, in an increase in genetic merit in the line; the invention further relates to sorting a sperm cell sample from a male swine in one of the mating subtypes into one or more subpopulations of sperm cells, wherein a majority of sperm cells in a subpopulation of sperm cells bear X chromosomes or Y chromosomes, and inseminating one or more female swine in the one of the mating subtypes with the subpopulation of sperm cells to achieve the percentage of progeny that are male, or the percentage of progeny that are female, determined to increase genetic merit relative to the control.
    Type: Application
    Filed: November 26, 2013
    Publication date: May 22, 2014
    Applicant: INGURAN, LLC
    Inventors: Hein van der Steen, Gregg BeVier
  • Patent number: 8709825
    Abstract: A nozzle for use in a flow cytometer and a method of orienting particles with a baffle disposed within a nozzle. The baffle may be a baffle plate positioned in a fluid flow path to deflect a fluid stream and to orient particles in a desired orientation as they exit the nozzle.
    Type: Grant
    Filed: March 1, 2013
    Date of Patent: April 29, 2014
    Assignee: Inguran, LLC
    Inventors: Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
  • Patent number: 8709817
    Abstract: A flow cytometry system and method for sorting a mixture of particles, such as a fluid switching sorting system for sorting selected particles which includes a control having a microprocessor programmed with instructions for implementing a control strategy for controlling the fluid delivery to the system to vary the rate at which fluid is delivered as a function of the purity or yield of a sub-population of particles.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: April 29, 2014
    Assignee: Inguran, LLC
    Inventors: Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak