Abstract: Provided are an improved genome editing system and a method that has high specificity, which are capable of obtaining stable mutation types.

Abstract: Provided relates to the field of genetic engineering. In particular, a method for base editing in plant is provided. More specifically, a highly efficient A to G base editing method for a target sequence in a genome of a plant (e.g., a crop plant) by a guide RNA-directed CRISPR-adenine deaminase fusion protein and the plant and their progeny produced by the method are provided.

Abstract: The invention relates to genetically modified plants with an altered seed phenotype, in particular increased seed size. The invention relates to a plant that does not produce a functional NGAL2 polypeptide or functional NGAL2 and NGAL3 polypeptides. NGAL2 and NGAL3 are members of the RAV family and comprise a B3 DNA-binding domain and a transcriptional repression motif.

Abstract: The present invention relates to the field of genetic engineering. In particular, the present invention relates to a genome editing method with high efficiency and high specificity. More specifically, the present invention relates to a method for increasing the efficiency of site-directed modification of a target sequence in a genome of an organism by a high-specificity Cas9 nuclease variant.

Abstract: The present invention provides a mutant wheat plant resistant to powdery mildew and producing method thereof, wherein the mutant wheat plant comprises a loss of function mutation in a TaMLO-A1, TaMLO-B1 and TaMLO-D1 nucleic acid sequence. The present invention also provides a method for determining the presence or absence of a mutant TaMLO-A1, TaMLO-B1 and TaMLO-D1 nucleic acid or polypeptide in a wheat plant.

Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for base editing in plants. More particularly, the invention relates to a method for performing efficient base editing to a target sequence in the genome of a plant (such as a crop plant) by a Cas9-cytidine deaminase fusion protein, as well as plants produced through said method and progenies thereof.

Abstract: This invention relates to a plant E3 ubiquitin ligase (termed DA2) which acts synergistically with DA1 to control seed and organ size. Methods of increasing plant yield are provided that comprise reducing the expression or activity of DA2 in a plant that is deficient in DA1 expression or activity. Plants with increased yield and methods of producing such plants are also provided.

Abstract: Methods and compositions that affect yield and other agronomic characteristics in plants are disclosed. Methods of transgenic modulation and marker-assisted breeding methods improve grain size and grain yield in rice are also disclosed. Increased expression of BG1 results in increased grain size and yield.

Abstract: The present invention discloses a method for obtaining glyphosate-resistant rice by a site-directed nucleotide substitution, and also relates to a method capable of generating a site-directed nucleotide substitution and a fragment substitution. The method for obtaining a glyphosate-resistant plant provided by the present invention comprises the following steps: only substituting threonine (T) at position 8 of the amino acid sequence of a conserved region of endogenous EPSPS protein of a target plant with isoleucine (I), and substituting proline (P) at position 12 with serine (S) to obtain a plant, i.e., a glyphosate-resistant plant. The method provided by the present invention is of great significance in breeding new herbicide-resistant plant varieties.

Abstract: The present invention discloses a method for improving the ability to resist against an intrusive DNA virus of a plant. The present invention provides a method for making a plant with improved ability to resist against a DNA virus, specifically can comprising the following steps: 1) selecting a sequence comply with 5?-NX-NGG-3? or 5?-CCN-NX-3? from the genomic sequence of the DNA virus as the target sequence; designing a DNA sequence reverse-complementary to said target sequence; 2) constructing said DNA sequence to a vector for expressing CRISPR/Cas9 nuclease so as to obtain a recombinant vector capable of transcribing a guide RNA and expressing a Cas9 protein; the crRNA in said guide RNA contains a RNA fragment transcribed from said DNA fragment; 3) introducing said recombinant vector into a recipient plant so as to obtain a plant with improved ability to resist against said DNA virus.

Abstract: The invention disclosed a method for conducting site-directed modification to a plant genome using non-inheritable materials. The method provided in the present invention specifically comprises the following steps: introducing a non-inheritable material into a cell or a tissue or a part of the plant of interest; wherein said non-inheritable material is a nuclease specific to said target fragment or an mRNA expressing said nuclease, thereby the target fragment is cleaved by said nuclease and site-directed modification to the target fragment is achieved through DNA repairing in the plant. By introducing a non-inheritable material of sequence-specific nuclease, site-directed mutation in a plant gene can be achieved, and no exogenous gene or nucleic acid fragments will be integrated into the plant as obtained. Therefore, the present invention can lead to more precise genome function study and higher biosafety in breeding.

Abstract: Provided is a method for conducting site-specific modification in a plant through gene transient expression, comprising the following steps: transiently expressing a sequence-specific nuclease specific to the target fragment in the cell or tissue of the plant of interest, wherein the sequence-specific nuclease is specific to the target site and the target site is cleaved by the nuclease, thereby the site-specific modification of the target site is achieved through DNA repairing of the plant.

Abstract: This invention relates to the identification of a regulator protein (termed CYP78A6, or EOD3) which controls the size of plant seeds and organs in Arabidopsis and other plants. Manipulation of CYP78A protein expression may useful, for example, in improving crop yield and increasing plant biomass.

Abstract: Methods for reprogramming cells by culturing the cells under a condition that allows formation of a three-dimensional cell aggregate are provided. The cells and cell aggregates obtained using the methods are also provided.

Abstract: The present invention discloses a protein IPA1 related to plant architecture, its coding genes and uses, wherein the protein is 1) or 2) as follows: 1) the protein consisting of the amino acid sequence showed by sequence 1 in the sequence list; 2) the protein derived from the protein of 1) by substitution and/or deletion and/or addition of one or several amino acid residues in the amino acid sequence defined in 1) and relating to plant architecture. IPA1 gene can be used for molecular marker-assisted breeding, cultivating new rice varieties and improving rice yield.

Abstract: Methods for reprogramming cells by culturing the cells under a condition that allows formation of a three-dimensional cell aggregate are provided. The cells and cell aggregates obtained using the methods are also provided.

Abstract: Provided are activated collagen scaffold materials as well as their special fused active restoration factors useful for promoting tissue repair, such as bone damage repair or nerve injury repair. The special fused active restoration factors are fusion proteins comprising a collagen-binding domain (CBD) at N-/C-terminus of cytokines, wherein the collagen-binding domain is a polypeptide consisting of 7-27 amino acid residues with a conservative sequence shown in SEQ ID NO:1 at N-terminus.

Abstract: The present invention discloses a protein IPA1 related to plant architecture, its coding genes and uses, wherein the protein is 1) or 2) as follows: 1) the protein consisting of the amino acid sequence showed by sequence 1 in the sequence list; 2) the protein derived from the protein of 1) by substitution and/or deletion and/or addition of one or several amino acid residues in the amino acid sequence defined in 1) and relating to plant architecture. IPA1 gene can be used for molecular marker-assisted breeding, cultivating new rice varieties and improving rice yield.

Abstract: Compositions and methods for imparting stress tolerance to plants using WRKY nucleic acid and polypeptides.

Abstract: Compositions and methods for conferring drought and salt tolerance to plants using tocopherol cyclase (TC) genes, including polynucleotides, polypeptides, vectors, cells and plants.