Abstract: The present invention provides an analysis method by which a DNA element can be identified with higher sensitivity, and a transcription amount of the DNA element can be determined.

Abstract: The present invention provides a method for analyzing a template nucleic acid, a method for analyzing a target substance, an analysis kit for a template nucleic acid or a target substance, and an analyzer for a template nucleic acid or a target substance, which are excellent in accuracy.

Abstract: The present invention is intended to provide a novel fluorescent labeled single-stranded nucleic acid, by which the background of an exciton oligomer can be further reduced and the novel use thereof. The present invention relates to a labeled single-stranded nucleic acid having at least two fluorescent atomic group pairs that exhibit an exciton effect. The labeled single-stranded nucleic acid is characterized in that the emission peak wavelength of one of the fluorescent atomic group pairs (fluorescent atomic group pair A) is shorter than the excitation peak wavelength of the other fluorescent atomic group pair (fluorescent atomic group pair B), and the fluorescent atomic group pairs A and B have a Förster resonance energy transfer (FRET) effect. This fluorescent labeled single-stranded nucleic acid is usable as a primer for amplifying a target nucleic acid or a probe to be hybridized with a target nucleic acid.

Abstract: The present invention provides a compound represented by the following chemical formula (I); a tautomer or stereoisomer of the compound; or a salt of the compound, the tautomer, or the stereoisomer. In the chemical formula (I), R1 and R2 are each a Group 1 element or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R1 and R2 together may form a protecting group of an amino group. R3 is a Group 1 element or a protecting group of a hydroxy group. R4 is a Group 1 element or —PR5R6R7R8 (R5, R6, R7, and R8 are each a Group 1 element, a lone electron pair, a Group 16 element, a Group 17 element, or a protecting group of a phosphorus atom, and may be identical to or different from each other). J is a hydrogen atom or an arbitrary atomic group, A is a hydrogen atom, a hydroxy group, an alkyl group, an aralkyl group, an alkoxy group, an electron-withdrawing group, a silylene group, or a sulfide group, or alternatively, J and A together may form a linker.

Abstract: The present invention provides a method for analyzing a template nucleic acid, a method for analyzing a target substance, an analysis kit for a template nucleic acid or a target substance, and an analyzer for a template nucleic acid or a target substance, which are excellent in accuracy.

Abstract: The present invention provides a miniaturizable tool that can analyze a target in a sample by a simple operation and an analysis method using the tool. An analysis chip is a syringe-type chip including: a syringe main body having an openable and closable tip; and a piston to be inserted into the syringe main body, the piston being a hollow body having a translucent closed-end at a side to be inserted into the syringe main body and an open-end at the other side.

Abstract: The present invention is to provide a method for analyzing a target nucleic acid, by which the target nucleic acid can be analyzed rapidly and easily. In order to achieve the above object, the present invention provides a method for analyzing a target nucleic acid in a sample, including the step of: analyzing the target nucleic acid in the sample by bringing the sample into contact with a label and with a primer or probe that can hybridize to the target nucleic acid. The primer or probe is immobilized on a solid phase. The label does not emit light when the primer or probe does not hybridize to the target nucleic acid, whereas the label emits light when the primer or probe has hybridized to the target nucleic acid. The analysis is carried out by detecting the light emitted from the label.

Abstract: The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.

Abstract: The present invention provides a tool that can analyze a target in a sample by a simple operation and can be downsized and an analysis method using the tool. By inserting an analysis chip into an analysis device, a reaction of a sample and a reagent is analyzed. The analysis chip includes a parallel flow channel in which plural reaction flow channels are connected in parallel, and the axial direction of the parallel flow channel is a direction of inserting the analysis chip into the analysis device.

Abstract: The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3? side thereof, a sequence (A?) that can hybridize to a sequence (A) on the 3? side of the target nucleic acid sequence. The second primer 1R includes, on the 3? side thereof, a sequence (B?) that can hybridize to a sequence (B) on the 3? side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5? sides thereof, sequences (C) that are substantially identical to each other.

Abstract: The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa.

Abstract: The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3? side thereof, a sequence (A?) that can hybridize to a sequence (A) on the 3? side of the target nucleic acid sequence. The second primer 1R includes, on the 3? side thereof, a sequence (B?) that can hybridize to a sequence (B) on the 3? side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5? sides thereof, sequences (C) that are substantially identical to each other.

Abstract: The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa.

Abstract: A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3? end portion, a sequence (Ac?) that hybridizes to a sequence (A) located in the 3? end portion of the target nucleic acid sequence. The first primer also contains, on the 5? side of the sequence (Ac?), a sequence (B?) that hybridizes to a complementary sequence (Bc) to a sequence (B) that is present on the 5? side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3? end portion, a sequence (Cc?) that hybridizes to a sequence (C) located in the 3? end portion of a complementary sequence to the target nucleic acid sequence.

Abstract: The present invention provides a primer that effectively can detect, for example, the double helix structure of a nucleic acid. The primer is a labeled nucleic acid containing at least one structure represented by the following formula (16), where B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 are each an atomic group that exhibits fluorescence and are identical to or different from each other.

Abstract: A method for determining the presence or absence of a mutation on the basis of the presence or absence of amplification with high reliability is provided. A target sequence including a target site contained in a sample nucleic acid is amplified using a primer that can hybridize to a region including the target site contained in the sample nucleic acid in the presence of a novel MutS having an amino acid sequence of SEQ ID NO: 2, and then the presence or absence of a mutation at the target site is determined on the basis of the presence or absence of amplification. The novel MutS binds more specifically to a mismatched base pair than to a fully-matched base pair, whereby an extension reaction caused by a mismatch-binding primer is suppressed. Thus, according to the present invention, the presence or absence of a mutation can be determined with high reliability.

Abstract: The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. In the process according to the present invention, a primer comprising in its 3?-end portion a sequence (Ac?) which hybridizes a sequence (A) in the 3?-end portion of the target nucleic acid sequence, and in the 5?-side of said sequence (Ac?) a sequence (B?) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5?-side of said sequence (A) on the target nucleic acid sequence, wherein {X?(Y?Y?)}/X is in the range of ?1.00 to 1.00, in which X denotes the number of bases in said sequence (Ac?), Y denotes the number of bases in the region flanked by said sequences (A) and (B) in the target nucleic acid sequence, and Y? denotes the number of bases in an intervening sequence between said sequences (Ac?) and (B?) (Y? may be zero).

Abstract: The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. The process involves providing a primer comprising in its 3?-end portion a sequence (Ac?) which hybridizes a sequence (A) in the 3?-end portion of the target nucleic acid sequence, and in the 5?-side of the sequence (Ac?) a sequence (B?) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5?-side of the sequence (A) on the target nucleic acid sequence, wherein {X?(Y?Y?)}/X is in the range of ?1.00 to 1.00, in which X denotes the number of bases in the sequence (Ac?), Y denotes the number of bases in the region flanked by the sequences (A) and (B) in the target nucleic acid sequence, and Y? denotes the number of bases in an intervening sequence between the sequences (Ac?) and (B?) (Y? may be zero).

Abstract: A DNA polymerase suitable for specific isothermal amplification methods and an isothermal amplification method using the DNA polymerase are provided. In the presence of a DNA polymerase including a protein described in the following item (a) or (b), an amplification reaction of a target nucleic acid sequence in a nucleic acid sample is carried out isothermally using a first primer shown in the following (X). By using the DNA polymerase, it becomes possible to carry out the amplification reaction using the primer within a shorter time than ever before. (a) a protein having an amino acid sequence represented by SEQ ID NO. 23 (b) a protein having an amino acid sequence represented by SEQ ID NO.

Abstract: The present invention provides a primer that effectively can detect, for example, the double helix structure of a nucleic acid. The primer is a labeled nucleic acid containing at least one structure represented by the following formula (16), where B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 are each an atomic group that exhibits fluorescence and are identical to or different from each other.