Abstract: A method of analyzing a biosample that enables substantial shortening of time required for analysis, further enabling obtaining highly reliable results through means for avoiding sway of analytical results depending on observers, and that enables one-time analysis of a multiplicity of genes, etc. on a single biosample to thereby enhance workload and time efficiencies, and that enables analysis of multiple genes, etc. under conditions completely free from any difference in background attributed to biosamples. There is provided a method comprising irradiating a biosample as an analyte with ultra-short pulse laser beams to thereby effect an ablation thereof so that molecules contained in the biosample are atomized into constituting elements, ionizing the constituting elements resulting from the atomization and analyzing the ionized constituting elements to thereby analyze analytical-target molecules of the biosample.
Type:
Application
Filed:
March 29, 2005
Publication date:
January 24, 2008
Applicants:
RIKEN, Kabushiki Kaisha Dnaform
Inventors:
Yoshihide Hayashizaki, Jun Kawai, Toshizo Hayashi, Mamoru Kamiya
Abstract: A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3? end portion, a sequence (Ac?) that hybridizes to a sequence (A) located in the 3? end portion of the target nucleic acid sequence. The first primer also contains, on the 5? side of the sequence (Ac?), a sequence (B?) that hybridizes to a complementary sequence (Bc) to a sequence (B) that is present on the 5? side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3? end portion, a sequence (Cc?) that hybridizes to a sequence (C) located in the 3? end portion of a complementary sequence to the target nucleic acid sequence.
Abstract: A novel protein which binds with tumor necrosis factor receptor associated factor 2, TRAF2, as well as a nucleic acid coding for the protein is disclosed. From a mouse cDNA library, a cDNA coding for a novel protein which binds with TRAF2 was discovered by mammalian two-hybrid assay, the cDNA was sequenced, the protein encoded by the cDNA was produced, and the fact that the protein binds with TRAF2 was experimentally confirmed.
Type:
Application
Filed:
January 22, 2003
Publication date:
February 22, 2007
Applicants:
The Institute of Physical and Chemical, Kabushiki Kaisha Dnaform
Abstract: Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution to form a bound DNA-carrier. The method also includes separating the DNA-bound carrier from other components. The method further includes dissociating the bound DNA from the DNA-binding carrier. The method still further includes recovering dissociated DNA.
Abstract: Abstract of the Disclosure A printed material comprising at least one support having at least one oligomer and/or polymer applied thereon is provided. Also, a method for preparing the printed material and a method for delivering and storing at least one oligomer and/or polymer are provided. The printed materials of the present invention are useful in providing scientists with oligomers and/or polymers of interest from the printed materials easily and immediately.