Abstract: The present invention is concerned with PCR-based detection methods and kits for the identification, differentiation, and quantification of different bacterial strains (e.g., Gram-negative bacterial strains), and also association of two or more PCR-positive genes to a single genome. The methods generally comprise carrying out PCR reactions using at least a first PCR primer set and/or probe for at least one target nucleic acid; and a second PCR primer set and/or probe for at least a second target nucleic acid. Positive PCR reaction products are then detected to determine test samples containing positive PCR reaction products for both the first and second target nucleic acids. This information can be used to calculate the gene association rate to determine whether the sample contains, for example, Shiga toxin-producing E. coli of the O-type serogroup.
Type:
Grant
Filed:
January 15, 2016
Date of Patent:
March 19, 2019
Assignee:
KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
Abstract: The present invention relates to protecting against, treating, and detecting Fusobacteria infections. Compositions and methods derived from nucleic acid and protein sequences of a 40 kDa Adhesin protein are provided to protect against, treat, and detect Fusobacteria infections in a subject. In one aspect, vaccines capable of inducing an immune response to a 40 kDa Adhesin protein are used to protect against Fusobacteria infection. Also, nucleic acid molecules, proteins, immunogens, antibodies, and antisense molecules derived from the sequences of the 40 kDa Adhesin protein may be used to protect against, treat, and detect Fusobacteria infections in a subject.
Type:
Grant
Filed:
April 25, 2018
Date of Patent:
March 5, 2019
Assignee:
Kansas State University Research Foundation
Inventors:
Sanjeev Narayanan, Amit Kumar, Tiruvoor Nagaraja, Muckatira Chengappa
Abstract: Rapid, point of care semi-quantitative tests for viral or bacterial antibody titer, and methods and kits for carrying out the same. The test article comprises a first elongated solid support with a sample application region and a detection region. The detection region comprises immobilized antigen that specifically binds a minimum immunoprotective level of antibody to at least one pathogen of interest. Depending upon the test results, the assay can rapidly indicate whether the animal has the presumptive minimally-protective level of antibody to the target pathogen, or needs to be re-vaccinated.
Type:
Grant
Filed:
August 18, 2015
Date of Patent:
December 18, 2018
Assignee:
KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
Abstract: Isolated porcine epidemic diarrhea virus (PEDV) deposited under ATCC Accession No. PTA-121847, and attenuated strains generated by serial passage in culture of the deposited strain. Immunogenic compositions for reducing the incidence or severity of clinical symptoms from PEDV infection, and methods of making and using the same.
Type:
Grant
Filed:
February 3, 2016
Date of Patent:
December 4, 2018
Assignee:
KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
Inventors:
Kyeong-Ok Chang, Yunjeong Kim, Richard A. Hesse
Abstract: Immunocompetent animal models having immunological tolerance to a xenograft and methods of producing the same. The animal models are tolerized to the xenograft in the pre-immune, fetal or embryonic stage, followed by transplanting the xenograft into the animal in the post-natal stage, such that the post-natal animal is immunologically tolerant to the xenograft, while remaining immunocompetent.
Type:
Grant
Filed:
April 21, 2016
Date of Patent:
November 13, 2018
Assignee:
Kansas State University Research Foundation
Inventors:
Deryl Troyer, Duane Davis, Stefan Bossmann
Abstract: Polymer-derived ceramic composites are described herein. The composites are formed using hexagonal boron nitride nanosheet-functionalized silicon-based ceramic precursor polymers. The composites a matrix of a polymer-derived ceramic and hexagonal boron nitride nanosheets embedded therein. Silicon-derived ceramic precursors such as polysilazane and/or polysiloxane are used to create improved SiCN and/or SiOC ceramic composites.
Type:
Grant
Filed:
July 8, 2015
Date of Patent:
October 9, 2018
Assignee:
Kansas State University Research Foundation
Abstract: A wireless power transfer system including a transmitting antenna with a first conductive helix wound about a first helical axis, and a first ground plane positioned adjacent to and angled orthogonally with the first helical axis. The wireless power transfer system additionally includes a receiving antenna with a second conductive helix wound about a second helical axis, and a second ground plane positioned adjacent to and angled orthogonally with the second helical axis. Furthermore, the wireless power transfer system further includes a conductive material connecting the first ground plane to the second ground plane.
Type:
Grant
Filed:
May 19, 2014
Date of Patent:
August 14, 2018
Assignee:
Kansas State University Research Foundation
Inventors:
Saeed M. Khan, Nathan Maresch, Justin Kuntz
Abstract: The present invention relates to protecting against, treating, and detecting Fusobacteria infections. Compositions and methods derived from nucleic acid and protein sequences of a 40 kDa Adhesin protein are provided to protect against, treat, and detect Fusobacteria infections in a subject. In one aspect, vaccines capable of inducing an immune response to a 40 kDa Adhesin protein are used to protect against Fusobacteria infection. Also, nucleic acid molecules, proteins, immunogens, antibodies, and antisense molecules derived from the sequences of the 40 kDa Adhesin protein may be used to protect against, treat, and detect Fusobacteria infections in a subject.
Type:
Grant
Filed:
November 28, 2017
Date of Patent:
July 3, 2018
Assignee:
Kansas State University Research Foundation
Inventors:
Sanjeev Narayanan, Amit Kumar, Tiruvoor Nagaraja, Muckatira Chengappa
Abstract: Methods of characterizing nanoparticles, nanoparticle complexes, and their biomolecular interactions are provided. Concurrent excitation and emission wavelength scans are performed and the optimal fluorescence intensity for the intersect of these wavelengths is determined. The intersect is dependent upon the physical characteristics of the particle and can be used to verify, for example, attachment of biomolecules, amount of biomolecules present, and structure of the attached biomolecule.
Type:
Grant
Filed:
November 4, 2016
Date of Patent:
May 22, 2018
Assignee:
Kansas State University Research Foundation
Abstract: A neutron detection apparatus includes a neutron detector and an analyzer. The neutron detector includes a plurality of neutron detector assemblies, where each of the neutron detector assemblies includes a plurality of neutron detection devices. The neutron detector also includes a moderating volume. The plurality of neutron detector assemblies are disposed within the moderating volume so as to form a three-dimensional array of neutron detection devices within the moderating volume. The analyzer is communicatively coupled to each of the neutron detection devices of the plurality of neutron detector assemblies. The analyzer configured to receive one or more measured response signals from each of the neutron detection devices, and perform one or more analysis procedures to determine one or more characteristics associated with the one or more neutron sources based at least on the received one or more measured response signals.
Type:
Grant
Filed:
October 10, 2016
Date of Patent:
May 1, 2018
Assignees:
The Curators of the University of Missouri, Kansas State University Research Foundation
Inventors:
Steven L. Bellinger, Simon Bolding, Anthony N. Caruso, Brian Cooper, Joseph A. Crow, James Currie, Ryan G. Fronk, Cory B. Hoshor, Douglas S. McGregor, William H. Miller, Eliot R. Myers, Thomas M. Oakes, Brent J. Rogers, John K. Shultis, Philip B. Ugorowski, Stephen M. Young
Abstract: Pharmaceutical or veterinary compositions, vaccine systems, methods, and kits for treating or protecting a subject from a condition using peptide-based adjuvants are provided. The peptide adjuvants comprise a peptide having a hydrophobic region, a turning region, and a hydrophilic region. The turning region comprises amino acid residues GSII (SEQ ID NO: 10). The peptide adjuvants can be used to immunopotentiate active agents by improving the immune response to the active agent.
Type:
Grant
Filed:
May 25, 2012
Date of Patent:
April 17, 2018
Assignee:
KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
Abstract: Disclosed is a dsRNA construct used to silencing specific eukaryotic translation initiation factor in plants to produce a plant resistant to viruses such as Potyviruses, Luteoviruses, and Furoviruses. More specifically, the plant would be resistant to viruses such as Wheat streak mosaic virus, Triticum mosaic virus, Soil bourne mosaic virus, or Barley yellow dwarf virus. Also disclosed are non-transgenic wheat plants having the genes for eIF(iso)4E-2 or eIF4G silenced.
Type:
Grant
Filed:
September 24, 2014
Date of Patent:
March 6, 2018
Assignees:
The United States of America, as represented by The Secretary of Agriculture, Kansas State University Research Foundation
Inventors:
John Fellers, Harold N. Trick, Luisa Cruz, Jessica Rupp
Abstract: Methods for synthesizing aluminum-modified silazanes, their use as polymer-derived ceramic precursors, and polymer-derived ceramics and carbon nanotube core/shell nanocomposites formed therefrom are disclosed.
Type:
Grant
Filed:
July 8, 2015
Date of Patent:
March 6, 2018
Assignee:
Kansas State University Research Foundation
Abstract: The present invention relates to protecting against, treating, and detecting Fusobacteria infections. Compositions and methods derived from nucleic acid and protein sequences of a 40 kDa Adhesin protein are provided to protect against, treat, and detect Fusobacteria infections in a subject. In one aspect, vaccines capable of inducing an immune response to a 40 kDa Adhesin protein are used to protect against Fusobacteria infection. Also, nucleic acid molecules, proteins, immunogens, antibodies, and antisense molecules derived from the sequences of the 40 kDa Adhesin protein may be used to protect against, treat, and detect Fusobacteria infections in a subject.
Type:
Grant
Filed:
February 24, 2016
Date of Patent:
February 6, 2018
Assignee:
Kansas State University Research Foundation
Inventors:
Sanjeev Narayanan, Amit Kumar, Tiruvoor Nagaraja, Muckatira Chengappa
Abstract: Attenuated vaccines to protect vertebrate animals and people against tick-born rickettsial, Ehrlichia and Anaplasma species infections is disclosed. Also disclosed are methods to modify the organism to achieve the desired immunity through the modification of a novel genetic region involved in pathogenesis. These compounds represent a needed vaccine against an organism causing life-threatening febrile illness in humans and animals, and also represent the potential to develop new classes of drugs targeting the gene products of genes Ech_0660, Ech_0379, and Ech_0230, and their homologs of other related rickettsial pathogens.
Type:
Application
Filed:
January 19, 2016
Publication date:
January 25, 2018
Applicant:
Kansas State University Research Foundation
Inventors:
Roman R. GANTA, Chuanmin CHENG, Arathy D. S. NAIR, Deborah JAWORSKI, Suhasini GANTA
Abstract: An electrochemical method for measuring the activity of enzymes using nanoelectrode arrays fabricated with vertically aligned carbon nanofibers. Short peptide substrates specific to disease-related enzymes are covalently attached to the exposed nanofiber tips. A redox moiety, such as ferrocene, can be linked at the distal end of the nanofibers. Contact of the arrays with a biological sample containing one or more target enzymes results in cleavage of the peptides and changes the redox signal of the redox moiety indicating the presence of the target enzymes.
Type:
Grant
Filed:
June 30, 2014
Date of Patent:
December 26, 2017
Assignee:
Kansas State University Research Foundation
Inventors:
Jun Li, Duy Hua, Lateef Uddin Syed, Allan Prior, Luxi Swisher
Abstract: Tricyclic pyrone compounds having high oral bioavailability, excellent blood-brain barrier permeability, and low toxicity are presented. Administration of the compounds to Alzheimer's Disease transgenic models resulted in substantially reduced soluble and insoluble A? species in the brain without affecting general behavior and motor coordination. Furthermore, in addition to blocking the toxicity and formation of both intraneuronal and extracellular A? aggregates, the compounds also increase cellular cholesterol efflux, restore axonal trafficking, and enhance hippocampal synaptic plasticity.
Type:
Grant
Filed:
September 30, 2014
Date of Patent:
October 31, 2017
Assignees:
KANSAS STATE UNIVERSITY RESEARCH FOUNDATION, THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, A CALIFORNIA CORPORATION, AFASCI, INC.
Inventors:
Duy H Hua, Lee-way Jin, Izumi Maezawa, Xinmin Xie
Abstract: The present invention describes subunit vaccines containing Gn and Gc glycoproteins of the Rift Valley Fever Virus, including nucleic acids encoding such glycoproteins, host cells, vectors, and immunoreagents generated with the glycoproteins, methods of vaccination, methods of diagnosis, and kits.
Type:
Grant
Filed:
January 28, 2014
Date of Patent:
October 17, 2017
Assignee:
Kansas State University Research Foundation
Inventors:
Juergen A. Richt, Bonto Faburay, William Wilson
Abstract: The present invention provides a diagnostic reagent or assay for assessing the activity of a protease in vivo or in vitro and methods of detecting the presence of a cancerous or precancerous cell. The assays are comprised of two particles linked via an oligopeptide linkage that comprises a consensus sequence specific for the target protease. Cleavage of the sequence by the target protease can be detected visually or using various sensors, and the diagnostic results can be correlated with cancer prognosis.
Type:
Grant
Filed:
January 26, 2015
Date of Patent:
August 15, 2017
Assignee:
Kansas State University Research Foundation
Inventors:
Stefan H. Bossmann, Deryl L. Troyer, Matthew T. Basel
Abstract: The present invention provides for compositions and methods for producing crop plants that are resistant to herbicides. In particular, the present invention provides for sorghum plants, plant tissues and plant seeds that contain altered acetyl-CoA carboxylase (ACC) genes and proteins that are resistant to inhibition by herbicides that normally inhibit the activity of the ACC protein.
Type:
Application
Filed:
April 3, 2017
Publication date:
July 20, 2017
Applicant:
Kansas State University Research Foundation