Patents Assigned to Life Technologies AS
  • Patent number: 12292408
    Abstract: Systems are provided for gel electrophoresis and electrotransfer that comprise one or more chambers that can removably and interchangeably receive either an electrophoresis cassette, or an electrotransfer cassette, and provide an electrical interface for both electrophoresis and electrotransfer of biomolecules. Electrophoresis devices including clamps and electrotransfer cassettes and related devices are provided. Methods for electrophoresis and electrotransfer using the systems and devices are also provided.
    Type: Grant
    Filed: July 12, 2021
    Date of Patent: May 6, 2025
    Assignees: Life Technologies Corporation, Life Technologies Holdings PTE Limited
    Inventors: Marie Murakami, Kyle Bulloch, Neil Olson, Ross Winnick, Wei Fuh Teo, Michael Thacker
  • Patent number: 12292125
    Abstract: A check valve assembly includes a first port fitting and a second port fitting with a base secured therebetween. The base has an annular sleeve encircling a seat, the seat having a central mounting hole and one or more flow channels passing therethrough. An umbrella valve includes a flexible sealing disk having an outer surface and an opposing inner surface, a mounting stem extending from the inner surface and projecting into the mounting hole of the base, the sealing disk being movable between a first position wherein at least a portion of the sealing disk sits on the seat so as to cover the one or more flow channels and a second position wherein the sealing disk is resiliently flexed so as to at least partially uncover the one or more flow channels. A retention plate is disposed between first port fitting and the seat of the base so that the retention plate sits against the outer surface of the sealing disk.
    Type: Grant
    Filed: December 7, 2023
    Date of Patent: May 6, 2025
    Assignee: Life Technologies Corporation
    Inventors: Charles R. Rhodes, Jonathan O. Schultz
  • Publication number: 20250137049
    Abstract: A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.
    Type: Application
    Filed: November 15, 2024
    Publication date: May 1, 2025
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventor: Earl Hubbell
  • Patent number: 12287307
    Abstract: A sensor device includes a substrate having a die attached to the substrate. The die includes an array of sensors and an array of wells cooperatively disposed over the array of sensors. The array of wells is exposed by the substrate. The sensor device further includes a flow cell secured to the substrate and defining a flow space disposed over the die and accessible to the array of wells. The flow cell defines a plurality of separate volumes. Each separate volume of the plurality of separate volumes has an inlet and an outlet. The plurality of separate volumes are separated by dividers. The dividers cover a set of wells of the array of wells.
    Type: Grant
    Filed: August 14, 2020
    Date of Patent: April 29, 2025
    Assignee: Life Technologies Corporation
    Inventors: Creighton Buie, Mark Reed, Albert Carrillo
  • Patent number: 12285713
    Abstract: A condenser bag includes a body bounding a channel that extends between an inlet opening at a first end and an outlet opening at an opposing second end, the body being comprised of a polymeric film; an intake port bounding a port opening that extends therethrough, the intake port being directly physically secured to the first end of the body so that the port opening communicates with the channel of the body; and a tubular transfer line having a first end directly mechanically coupled with the body at a first location located between the inlet opening and the outlet opening so as to communicate with the channel and an opposing second end directly mechanically coupled to the intake port so as to communicate with the port opening thereof.
    Type: Grant
    Filed: June 21, 2023
    Date of Patent: April 29, 2025
    Assignee: Life Technologies Corporation
    Inventors: Jason D. Brown, Clinton C. Staheli, Anthony D. Meeks
  • Patent number: 12280378
    Abstract: Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products.
    Type: Grant
    Filed: June 23, 2020
    Date of Patent: April 22, 2025
    Assignee: Life Technologies Corporation
    Inventor: Jason Yingjie Liu
  • Patent number: 12275997
    Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample.
    Type: Grant
    Filed: October 28, 2022
    Date of Patent: April 15, 2025
    Assignee: Life Technologies Corporation
    Inventors: Gordon A. Janaway, Mark Andersen, Kornelija Zgonc, Michael C. Pallas, Marcin Sikora, Casey R. Mcfarland, Ferrier N. Le, Haopeng Wang, Jian Gong, Gothami Padmabandu
  • Patent number: 12268711
    Abstract: Disclosed is a method for preparing a product for treating scald infection caused by Pseudomonas aeruginosa. The product includes a ferrous sulfate hydrogel, the scald is a deep second-degree scald or a third-degree scald, and a preparation process of the ferrous sulfate hydrogel includes: S1, obtaining a mixed solution by dissolving ferrous sulfate and vitamin C at a concentration ratio of 1:1 in sterile ultrapure water, heating the mixed solution to 50° C., and a concentration of the ferrous sulfate in the mixed solution being 2 mM, and S2, under a condition of heating, continuing to add sodium alginate powder whose mass constitutes 3.5% of a total mass of the mixed solution, stirring until the sodium alginate powder is dissolved and forming a stable colloid, and obtaining 2 mM of the ferrous sulfate hydrogel.
    Type: Grant
    Filed: July 19, 2024
    Date of Patent: April 8, 2025
    Assignee: NINGBO EISEN LIFE TECHNOLOGY CO., LTD.
    Inventors: Liangbin Hu, Haizhen Mo, Xiaohui Zhou, Hongbo Li, Dan Xu, Zhenbin Liu, Zhen Wang
  • Patent number: 12271043
    Abstract: Provided are optical fiber alignment assemblies, comprising: a plurality of fiber channel members being arranged along a first vertical axis, each fiber channel member comprising a fiber channel configured to accommodate an optical fiber disposed therein, the plurality of fiber channels being parallel to one another, fiber channels adjacent to one another defining a spacing therebetween, the spacing being measured along the first vertical axis; a plurality of resilient members, a resilient member being disposed between adjacent fiber channel members; and an adjustment element, the adjustment element being configured to effect a force oriented along the first vertical axis, and the adjustment member being configured such that actuation of the adjustment member changes a compression of the plurality of resilient members so as to effect an essentially linear variation in the spacing between adjacent fiber channels. Also provided are related methods.
    Type: Grant
    Filed: April 26, 2022
    Date of Patent: April 8, 2025
    Assignee: Life Technologies Corporation
    Inventors: Derek Robinson, Wesley Smith, Keith Kearsley
  • Patent number: 12272538
    Abstract: Disclosed herein are systems for imaging and ablating a sample. An imaging/ablating device (110) includes an optical assembly (112), a sample stage (114), and a receiver (116). The optical assembly (112) is disposed in an inverted position below the sample stage (114) and the receiver (116) is positioned above the sample stage (112). The optical assembly enables imaging of a sample disposed on the sample stage (114). The optical assembly (112) also enables ablation of a region of interest within the sample. The laser light propagated from the optical assembly during ablation propagates substantially in the same direction as the direction of travel of the ablation plume (20) toward the receiver (116).
    Type: Grant
    Filed: November 10, 2020
    Date of Patent: April 8, 2025
    Assignees: Thermo Fisher Scientific (Bremen) GmBH, Life Technologies Corporation, FEI Deutschland GmbH
    Inventors: Alexander Makarov, Michael Ward, Rainer Daum
  • Patent number: 12263475
    Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
    Type: Grant
    Filed: October 20, 2022
    Date of Patent: April 1, 2025
    Assignee: Life Technologies Corporation
    Inventors: Jonathan Schultz, Todd Roswech
  • Patent number: 12264363
    Abstract: A system for nucleic acid sequencing is provided. The system comprises a sequencing device configured to expose a tagged polynucleotide comprising a combinatorial barcode sequence and a sample polynucleotide to sequential nucleotide flows, each flow comprising one species of nucleotide and the sequential flows being in a predetermined order based on the species of nucleotide such that exposing of the tagged polynucleotide to the sequential nucleotide flows causes incorporations of nucleotides from the sequential nucleotide flows into the tagged polynucleotide over the barcode sequence The sequencing device is configured to detect a series of signals over the barcode sequence resulting from the incorporations, wherein the predetermined order of nucleotide flows comprises a repetition of a flow order motif that is based on a sequence motif. The system comprises a computing device configured to resolve the detected series of signals to determine the combinatorial barcode sequence.
    Type: Grant
    Filed: December 9, 2021
    Date of Patent: April 1, 2025
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Earl Hubbell
  • Patent number: 12262566
    Abstract: An optoelectronic module, including a substrate, a covering member, a light emitting element, and a light receiving element, is provided. The covering member is disposed on the substrate and includes an upper cover portion, a peripheral sidewall portion connected to the upper cover portion, and an inside partition delimiting a first cavity and a second cavity. The first cavity is separated from the second cavity. The light emitting element is disposed on the substrate as corresponding to the first cavity. The light receiving element is disposed on the substrate as corresponding to the second cavity. The inside partition has a first inner wall surface located in the first cavity and a second inner wall surface located in the second cavity. A first protruded-recessed structure is formed on the first inner wall surface.
    Type: Grant
    Filed: March 10, 2022
    Date of Patent: March 25, 2025
    Assignee: Life-On Technology Corporation
    Inventors: Jui Lin Tsai, Chien Tien Wang, Shu-Hua Yang, Hsin Wei Tsai, You-Chen Yu
  • Patent number: 12258477
    Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.
    Type: Grant
    Filed: August 8, 2019
    Date of Patent: March 25, 2025
    Assignee: Life Technologies Corporation
    Inventors: Jongtae Yang, Xin Wang, Yi-Zhen Hu, Kyle Gee, Aimei Chen, Aleksey Rukavishnikov
  • Patent number: 12258549
    Abstract: The present set of embodiments relate to a system, method, and apparatus for culturing cells within a cell culture vessel having a mixing element. The cell culture system includes a flexible portion and a mixing element disposed therein. The mixing element includes a suspended foldable portion. The system is configured to reduce shear stress on cells without compromising mixing efficiency. This reduction is accomplished by using a mixing element having a large surface area allowing for reduced rotational speeds. The system is collapsible for ease of transport and disposal. The flexible portion collapses and the foldable portion folds to minimize the volume of the system while not in operation.
    Type: Grant
    Filed: August 30, 2023
    Date of Patent: March 25, 2025
    Assignee: Life Technologies Corporation
    Inventor: Stephan Kaiser
  • Patent number: 12253447
    Abstract: An isolation device for isolation of target particles from a plurality of liquid samples includes a plurality of isolation chips and a vacuum system. Each of the plurality of isolation chips includes a sample reservoir, and a first outlet and a second outlet disposed at opposite sides of the sample reservoir. The vacuum system includes a first vacuum pump connected to the first outlet of each of the plurality of isolation chips and a second vacuum pump connected to the second outlet of each of the plurality of isolation chips. The first vacuum pump generates a negative pressure in each of the plurality of isolation chips through a corresponding first outlet. The second vacuum pump generates a negative pressure in each of the plurality of isolation chips through a corresponding second outlet. The target particles are isolated from each of the plurality of liquid samples in a corresponding sample reservoir.
    Type: Grant
    Filed: December 6, 2023
    Date of Patent: March 18, 2025
    Assignee: Shenzhen Huixin Life Technologies Co., Ltd
    Inventor: Yuchao Chen
  • Patent number: 12245854
    Abstract: Disclosed are devices and methods for collection, labeling and matching biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature such as a fingerprint or footprint of an individual. Such devices and methods are used in forensic, human identification, paternity, tissue typing, and screening technologies to rapidly process an individual's identity, determine the identity of an individual along with the genotype profile of the individual.
    Type: Grant
    Filed: November 2, 2021
    Date of Patent: March 11, 2025
    Assignee: Life Technologies Corporation
    Inventors: Michael Harrold, Lori Hennessy, Leonard Klevan
  • Patent number: 12241119
    Abstract: This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (PCR).
    Type: Grant
    Filed: May 25, 2022
    Date of Patent: March 4, 2025
    Assignee: Life Technologies Corporation
    Inventors: Jonathan Wang, David Dupont
  • Patent number: D1065223
    Type: Grant
    Filed: September 26, 2022
    Date of Patent: March 4, 2025
    Assignees: Life Technologies Corporation, Life Technologies Holding PTE Limited
    Inventors: Vi Ee Lau, Mio Xiu Lu Ling, Laurel Stone, Chrisgen Vonnegut, Andrew Dix, Alexia Bachir, Sneh Ringwala
  • Patent number: D1065578
    Type: Grant
    Filed: November 4, 2024
    Date of Patent: March 4, 2025
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Timothy Barta, John Staton, Robert Setterquist, Sandro Klein, Carolyn Johnson, Justin Inslee, Jason Jones, Christopher Crowley, Ethan Gentert, Wade Lukianow, Sara Thiele, Jay Greco, Syrus Jaffe, Eric Liu, Alexandre Vlassov, Marie Gonzalez, Christopher Martin, Robert Smith