Patents Assigned to Life Technologies Corporation
  • Patent number: 12054790
    Abstract: Methods, compositions and kits for detecting microorganisms and/or profiling microbiota such as for example through use of nucleic acid amplification and detection.
    Type: Grant
    Filed: April 23, 2020
    Date of Patent: August 6, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Ioanna Pagani, Pius Brzoska, Kelly Li, Sunali Patel, Boli Huang, Kamini Varma, Nitin Puri, Evan Diamond
  • Patent number: 12050197
    Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.
    Type: Grant
    Filed: February 14, 2022
    Date of Patent: July 30, 2024
    Assignee: Life Technologies Corporation
    Inventors: Melville Davey, Simon Cawley, Alan Williams
  • Patent number: 12050196
    Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or dis
    Type: Grant
    Filed: October 12, 2021
    Date of Patent: July 30, 2024
    Assignee: Life Technologies Corporation
    Inventors: Chiu Tai Andrew Wong, Todd Rearick, John Donohue
  • Patent number: 12050226
    Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.
    Type: Grant
    Filed: September 2, 2021
    Date of Patent: July 30, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: James A. Ball, Jonathan Schultz
  • Patent number: 12050195
    Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
    Type: Grant
    Filed: July 30, 2021
    Date of Patent: July 30, 2024
    Assignee: Life Technologies Corporation
    Inventors: Jonathan M. Rothberg, Todd Rearick
  • Patent number: 12038406
    Abstract: The described embodiments may provide a chemical detection circuit that may comprise a plurality of first output circuits at a first side and a plurality of second output circuits at a second side of the chemical detection circuit. The chemical detection circuit may further comprise a plurality of tiles of pixels each placed between respective pairs of first and second output circuits. Each tile may include four quadrants of pixels. Each quadrant may have columns with designated first columns interleaved with second columns. Each first column may be coupled to a respective first output circuit in first and second quadrants, and to a respective second output circuit in third and fourth quadrants. Each second column may be coupled to a respective second output circuit in first and second quadrants, and to a respective first output circuit in third and fourth quadrants.
    Type: Grant
    Filed: October 7, 2020
    Date of Patent: July 16, 2024
    Assignee: Life Technologies Corporation
    Inventors: Kim L. Johnson, Jeremy Jordan, Peter M Levine, Mark James Milgrew
  • Patent number: 12038405
    Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
    Type: Grant
    Filed: May 26, 2023
    Date of Patent: July 16, 2024
    Assignee: Life Technologies Corporation
    Inventors: Mark Milgrew, Jonathan Rothberg, James Bustillo
  • Patent number: 12040048
    Abstract: The method includes compressing numbers of reads data for targeted genes of a gene expression assay performed on a test sample. The targeted genes are organized into categories. Each category represents a functional context associated with the targeted genes in that category. The numbers of reads corresponding to targeted genes each category is compressed to form a compressed value for the category. The compressed value is compared to a baseline value for the category to determine an enrichment or a loss of a signature corresponding to the functional context of the category. The method may include analyzing information from multiple assays performed on the test sample, assigning a score value to each assay result and predicting a response to immune-oncology treatment based on the assigned scores.
    Type: Grant
    Filed: February 17, 2023
    Date of Patent: July 16, 2024
    Assignee: Life Technologies Corporation
    Inventors: Fiona Hyland, Asha Kamat, Timothy Looney
  • Patent number: 12031916
    Abstract: An instrument for processing and/or measuring a biological process comprises a sample processing system and an excitation source exhibiting a spectral function of output power or intensity verses wavelength of output power or intensity. The spectral function has a minima wavelength corresponding to a local minima value of the output power or intensity; a first maxima wavelength corresponding to a first local maxima of output power or intensity, the output power or intensity at the first local maxima being greater than the output power or intensity at any wavelength less than the minima wavelength; a second maxima wavelength corresponding to a second local maxima of output power or intensity, the output power or intensity at the second local maxima being greater than the output at any wavelength greater than the minima wavelength; the minima wavelength is between the first maxima wavelength and the second maxima wavelength.
    Type: Grant
    Filed: October 7, 2022
    Date of Patent: July 9, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mingsong Chen, Mauro Aguanno, Kuan Moon Boo, Woon Liang Terence Soh, Jacob K. Freudenthal, Jeffrey A. Marks
  • Patent number: 12012579
    Abstract: A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.
    Type: Grant
    Filed: October 10, 2022
    Date of Patent: June 18, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Clinton C. Staheli, Nephi D Jones, Michael E. Goodwin
  • Patent number: 12006543
    Abstract: A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.
    Type: Grant
    Filed: March 30, 2021
    Date of Patent: June 11, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Guobin Luo, Marina Sedova, David Light, Ryan Jones, Mohammad Alanjary
  • Patent number: 12005412
    Abstract: A method for mixing a fluid includes: dispensing a first volume of a fluid into a flexible container, the flexible container being at least partially disposed within the chamber of a support housing; repeatedly moving the support housing and the flexible container contained therein so as to mix the first volume of fluid within the flexible container; adding further fluid into the flexible container after moving the support housing to form a second volume of fluid; and manipulating a mixing element within the flexible container so as to mix the second volume of fluid.
    Type: Grant
    Filed: April 21, 2021
    Date of Patent: June 11, 2024
    Assignee: Life Technologies Corporation
    Inventors: Nephi D. Jones, Brandon M. Knudsen, Whitt F. Woods
  • Patent number: 12006536
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Grant
    Filed: August 11, 2021
    Date of Patent: June 11, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Linda Lee, Sam Woo, Peter Ma
  • Patent number: 11998910
    Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.
    Type: Grant
    Filed: August 26, 2021
    Date of Patent: June 4, 2024
    Assignee: Life Technologies Corporation
    Inventors: Mark Reed, Zheng (Derek) Xing, David Marran, Earl Zergiebel
  • Patent number: 12003145
    Abstract: A rotor assembly includes a rotor plate to rotate around a first axis, a bucket attached to the rotor plate and to rotate around a second axis, and a stop plate to rotate around the first axis between an open position and a closed position. When in the closed position, the stop plate engages the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly. The rotor assembly further includes a housing for a sensor array component, the housing disposed in the bucket and including a solution inlet, a solution outlet, a transfer basin, a solution retainer disposed between the solution outlet and the transfer basin, and a collection reservoir in fluid communication with the transfer basin. The solution inlet and the solution outlet to engage ports of a flow cell of a sensor array.
    Type: Grant
    Filed: February 2, 2023
    Date of Patent: June 4, 2024
    Assignee: Life Technologies Corporation
    Inventor: Todd Roswech
  • Patent number: 11999930
    Abstract: A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.
    Type: Grant
    Filed: June 16, 2023
    Date of Patent: June 4, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Michael E. Goodwin, Nephi D. Jones
  • Patent number: 11996169
    Abstract: In one exemplary embodiment, a method computer-implemented method of generating an outlier wheel data visualization for a graphical user interface (GUI) is provided. The method includes receiving emission data from a plurality of amplification reactions. The method further includes generating an outlier wheel data visualization using the emission data including a plurality of lines, where each line included in the outlier wheel data visualization represents emission data from an amplification reaction of the plurality of amplification reactions, and each line having a length and a visual indicator. The length of each line represents growth of intensity of fluorescence of the emission data and the visual indicator indicates the associated cycle number of the emission data. Further, each line is associated with an angular position so that the plurality of lines is configured in a circular shape. The method includes displaying the outlier wheel data visualization on a GUI.
    Type: Grant
    Filed: June 24, 2022
    Date of Patent: May 28, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Nivedita Sumi Majumdar
  • Patent number: 11994528
    Abstract: Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.
    Type: Grant
    Filed: December 15, 2020
    Date of Patent: May 28, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Alexandre Vlassov, Timothy Barta, Justin Inslee, Ethan Gentert, Robert Smith, Sara Thiele, Carolyn Adams, Kraig Kruger, Robert Schneider, Wade Lukianow, Jay Greco, Jason Jones, David Cerrone, Sumonto Ghosh, Brian Vajda, Andrew Basta, Allan Evans, Christopher Crowley, Robert Setterquist, Anne Cox, Mark Mayernick
  • Patent number: D1032646
    Type: Grant
    Filed: February 19, 2021
    Date of Patent: June 25, 2024
    Assignee: Life Technologies Corporation
    Inventors: Joshua Mead, Scott Rickes, Sean Zimmerman, Alessandra Barbosa, Mio Ling, Woon Liang Soh, Kevin Mullen, Christopher Brau, Jordan Cobia, Harmon Cosme Sicat, Jr.
  • Patent number: D1037486
    Type: Grant
    Filed: March 10, 2022
    Date of Patent: July 30, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kyle Bulloch, Xin Mathers