Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures.
Type:
Application
Filed:
January 21, 2011
Publication date:
June 2, 2011
Applicant:
LIFELINE SCIENTIFIC, INC.
Inventors:
Donald R. Owen, David C. Kravitz, John Brassil, Kelvin G.M. Brockbank, Andrew Burroughs, Dickon Isaacs, Douglas Schein
Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures.
Type:
Application
Filed:
November 5, 2010
Publication date:
March 10, 2011
Applicant:
LIFELINE SCIENTIFIC, INC.
Inventors:
Donald R. Owen, David C. Kravitz, John Brassil, Kelvin G.M. Brockbank, Andrew Burroughs, Dickon Isaacs, Dennis J. Steibel, Richard Fraser, Stanley Harris, Douglas Schein
Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures.
Type:
Application
Filed:
November 5, 2010
Publication date:
March 3, 2011
Applicant:
Lifeline Scientific, Inc.
Inventors:
Donald R. Owen, David C. Kravitz, John Brassil, Kelvin G.M. Brockbank, Andrew Burroughs, Dickon Isaacs, Dennis J. Steibel, Richard Fraser, Stanley Harris, Douglas Schein
Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures.
Type:
Application
Filed:
October 22, 2010
Publication date:
February 17, 2011
Applicant:
LIFELINE SCIENTIFIC, INC.
Inventors:
Donald R. OWEN, David C. Kravitz, John Brassil, Kelvin G.M. Brockbank, Andrew Burroughs, Dickon Isaacs, Dennis J. Steibel, Richard Fraser, Stanley Harris, Douglas Schein, Christopher G. Curtis
Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures.
Type:
Grant
Filed:
July 11, 2003
Date of Patent:
November 2, 2010
Assignee:
Lifeline Scientific, Inc.
Inventors:
Donald R. Owen, David C. Kravitz, John Brassil, Kelvin G. M. Brockbank, Andrew Burroughs, Dickon Isaacs, Dennis J. Steibel, Richard Fraser, Stanley Harris, Douglas Schein
Abstract: Method for preserving tissue including immersing the tissue in a solution having a cryoprotectant concentration of at least 75% by weight, a cooling step where the tissue is cooled to a temperature between the glass transition temperature of the solution having a cryoprotectant concentration of at least 75% by weight and ?20° C., a storage step where the tissue is stored at a temperature between the glass transition temperature of the solution and ?20° C., a rewarming step, where the tissue is warmed, and a washing step.
Type:
Application
Filed:
February 23, 2010
Publication date:
August 26, 2010
Applicants:
CELL & TISSUE SYSTEMS, INC., LIFELINE SCIENTIFIC INC.
Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and/or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and/or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures. During the period in which the organ is preserved and/or maintained, various drug research and development may be performed on and/or with the organ.
Type:
Grant
Filed:
February 2, 2004
Date of Patent:
July 6, 2010
Assignee:
Lifeline Scientific, Inc.
Inventors:
John Brassil, Douglas Schein, Christopher Curtis
Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The apparatus and methods include the cassette and transporter with heat transfer surfaces arranged to transfer heat between a cooling source in said transporter and the heat transfer surfaces of the cassette.
Type:
Application
Filed:
February 16, 2010
Publication date:
June 17, 2010
Applicant:
LIFELINE SCIENTIFIC, INC.
Inventors:
Jeffrey C. Garland, Douglas Schein, David Walter Wright
Abstract: An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The apparatus and methods include the cassette and transporter with heat transfer surfaces arranged to transfer heat between a cooling source in said transporter and the heat transfer surfaces of the cassette.
Type:
Grant
Filed:
April 2, 2004
Date of Patent:
April 6, 2010
Assignee:
Lifeline Scientific, Inc.
Inventors:
Jeffrey C. Garland, Douglas Schein, David Walter Wright
Abstract: A system and method for regulating cooperatively the pressures and flows of input vessels such as both the portal vein and hepatic artery for the liver. This invention solves problems of less-than-therapeutic portal vein flow during perfusion preservation by implementing cooperative regulation between the inputs, e.g., portal vein and hepatic artery pumping systems, on an organ preservation apparatus. It includes an algorithm that adapts to the situation wherein the portal vein has reached minimum flow and maximum pressure. The cooperative regulation algorithm senses the problem with the portal vein and solves it by adjusting the hepatic artery flow conditions.
Abstract: Methods of validating results of assessments of test substances using human-derived tissues and/or organs, particularly tissues and/or organs unsuitable for transplantation, include assessing the suitability of the organ and/or tissue for substance testing, as well as inter-organ variability and use of exogenous and/or endogenous controls.
Type:
Application
Filed:
May 18, 2007
Publication date:
November 20, 2008
Applicant:
Lifeline Scientific, Inc.
Inventors:
Christopher Gerald Curtis, John Brassil, David Kravitz
Abstract: Properties such as toxicity of substances may be determined by assaying properties, fates and effects of substances in an ex vivo metabolically active human organ or tissue under normothermic perfusion with a fluid containing a test substance. The data can be used as, for example, part of a submission to a government regulatory organization. Preferred methods use perfused endocrine gland organs or tissues to evaluate hormone or other bodily chemical disruption caused by substances and pre-donation diseased or injured organs or tissues.
Type:
Application
Filed:
May 18, 2007
Publication date:
November 20, 2008
Applicant:
Lifeline Scientific, Inc.
Inventors:
Christopher Gerald Curtis, John Brassil, David Kravitz