Abstract: Provided herein are engineered HSV-1 vectors comprising a modified HSV-1 genome. The engineered HSV-1 vectors can be used to deliver genetic circuits (e.g., up to 100 kb) to cells in vitro or in vivo. Methods of treating or diagnosing a disease (e.g., cancer) using the engineered HSV-1 vectors described herein are also provided.

Abstract: System and methods for communicating across a network comprise: a database containing high level security rules for the network; computing devices communicating on the network; a security rule translation module; event sensors configured to monitor and detect one or more events occurring on or relating to the network, and in response thereto, provide to the security rule translation module an indication of occurrence for each of the one or more security events. The security rule translation module may associate the security rules with the security events corresponding to the received indication, and produce a low-level security rule based on data from the high-level security rule and the received indication of occurrence of the security events. The system may also include switches coupled to receive the low-level security rules from the security rule translation module and enforce the low-level security rules on the network.

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

Abstract: Provided herein is a pathway for adipocyte energy consumption regulation involving ARID5B, genetic variant rs1421085, IRX3, and IRX5. Compositions and methods for modulating the pathway in vitro and in vivo for anti-cachectic and anti-obesity effects are provided. Methods of identifying subjects at risk of developing a disorder mediated by a dysregulation of the energy consumption pathway are also provided.

Abstract: A self-sealing container, used for transferring one or more air sensitive samples between vacuum systems, includes a housing with a cavity that holds the sample(s), and a lid disposed on top of the housing. The lid is configured to interface with the housing to form a seal over the cavity when the lid is closed. At least two flexure systems, each coupled to the housing and the lid, are disposed on opposing sides of the housing. The flexure systems apply forces to the lid that move the lid horizontally and vertically above the housing, into an open position. A pneumatic actuator, movably coupled to the housing and the lid, that is actuated by a change in ambient pressure. When actuated, it applies forces that move the lid horizontally and vertically to the closed position, in opposition to and in excess of the forces applied by the flexure systems.

Abstract: Pharmaceutical compositions including an effective amount of an antiandrogen or androgen antagonist in combination with a Plk inhibitor and methods of use thereof for treating cancer are disclosed. Administration of the combination of the active agents can be effective to reduce cancer cell proliferation or viability in a subject with cancer to the same degree, or a greater degree than administering to the subject the same amount of either active agent alone. The active agents can be administered together or separately. Methods of selecting and treating subjects with cancers, particular prostate cancers including castration resistant prostate cancer, breast cancers, particularly androgen receptor positive breast cancers, and pancreatic cancers are also provided.

Abstract: A shape-morphing ultralight structure using materials that dramatically increase the efficiency of load-bearing aerostructures that includes a programmable material system applied as a large-scale, ultralight, and conformable (shape-morphing) aeroelastic structure. The use of a modular, lattice-based, ultralight material results in stiffness and density typical of an elastomer. This, combined with a building block-based manufacturing and configuration strategy, enables the rapid realization of new adaptive structures and mechanisms. The heterogeneous design with programmable anisotropy allows for enhanced elastic and global shape deformation in response to external loading, making it useful for tuned fluid-structure interaction. The present invention demonstrates an example application experiment using two building block types for the primary structure of a 4.27 m wingspan aircraft with spatially programed elastic shape morphing to increase aerodynamic efficiency.

Abstract: The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a DNA-targeting Cpf1 protein, at least one guide molecule, and at least one adenosine deaminase protein or catalytic domain thereof.

Abstract: The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.

Abstract: Embodiments including apparatus, systems, and methods for nanofabrication are provided. In one example, a method of manufacturing a semiconductor device includes forming a two-dimensional (2D) layer comprising a 2D material on a first substrate and forming a plurality of holes in the 2D layer to create a patterned 2D layer. The method also includes forming a single-crystalline film on the patterned 2D layer and transferring the single-crystalline film onto a second substrate.

Abstract: Provided herein, in some aspects, are antibody expression systems comprising DNA launched RNA replicons for high level antibody expression. In some embodiments, the antibody is a therapeutic antibody. In some embodiments, the antibody is an immune check point inhibitor. Methods of using the antibody expression system for treating diseases (e.g., cancer) are also provided.

Abstract: The present disclosure relates to synthetic oncolytic viruses comprising a lipid nanoparticle comprising one or more types of lipid and a self-amplifying replicon RNA comprising a sequence that encodes an immunomodulatory molecule.

Abstract: In one aspect, embodiments disclosed herein are directed to engineered CRISPR-Cas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein that enhances binding of the of the CRISPR complex to the binding site and/or alters editing preference as compared to wild type. In certain example embodiments, the CRISPR-Cas effector proteins a Type II effector protein. In certain other example embodiments, the Type V effector protein is Cas9 or an orthologs or engineered variant thereof. Example Cas9 proteins suitable for use in the embodiments disclosed herein are discussed in further detail below.

Abstract: An imaging device is often paired with a readout integrated circuit (ROIC), which provides processing and data transfer functionality. The circuitry of a ROIC is typically specialized to meet the requirements of an application, which limits the ROIC to a few modes of operation and restricts compatibility to only certain types of imaging devices and applications. Furthermore, the circuitry supporting the processing functionality is limited due to size constraints on the ROIC. These shortcomings can be overcome with a field programmable imaging array (FPIA), which can be implemented as an integrated circuit combining customized ROIC sensor interface circuitry with field programmable gate array (FPGA) circuitry to enable post-fabrication definition of ROIC operational modes. An FPIA chip may form part of a three-chip stack that also includes an analog sensor interface chip for analog-to-digital conversion and an imaging device.

Abstract: A graphene-based layer transfer (GBLT) technique is disclosed. In this approach, a device layer including a III-V semiconductor, Si, Ge, III-N semiconductor, SiC, SiGe, or II-VI semiconductor is fabricated on a graphene layer, which in turn is disposed on a substrate. The graphene layer or the substrate can be lattice-matched with the device layer to reduce defect in the device layer. The fabricated device layer is then removed from the substrate via, for example, a stressor attached to the device layer. In GBLT, the graphene layer serves as a reusable and universal platform for growing device layers and also serves a release layer that allows fast, precise, and repeatable release at the graphene surface.

Abstract: An optical neural network is constructed based on photonic integrated circuits to perform neuromorphic computing. In the optical neural network, matrix multiplication is implemented using one or more optical interference units, which can apply an arbitrary weighting matrix multiplication to an array of input optical signals. Nonlinear activation is realized by an optical nonlinearity unit, which can be based on nonlinear optical effects, such as saturable absorption. These calculations are implemented optically, thereby resulting in high calculation speeds and low power consumption in the optical neural network.

Abstract: When a soliton and a dispersive pulse propagate in an optical fiber, they can interact via cross-phase modulation, which occurs when one pulse modulates the refractive index experienced by the other pulse. Cross-phase modulation causes each pulse to shift in wavelength by an amount proportional to the time delay between the pulses. Changing the time delay between the pulses changes the wavelength shift of each pulse. This make it possible to produce pulses whose output wavelengths can be tuned over large ranges, e.g. hundreds of nm, in a time as short as the pulse repetition period of the laser (e.g., at rates of megahertz or gigahertz). Such a laser requires no moving parts, providing high reliability. The laser's optical path can be made entirely of optical fiber, providing high efficiency with low size, weight, and power consumption.

Abstract: Conventional approaches to integrating waveguides within standard electronic processes typically involve using a dielectric layer, such as polysilicon, single-crystalline silicon, or silicon nitride, within the in-foundry process or depositing and patterning a dielectric layer in the backend as a post-foundry process. In the present approach, the back-end of the silicon handle is etched away after in-foundry processing to expose voids or trenches defined using standard in-foundry processing (e.g., complementary metal-oxide-semiconductor (CMOS) processing). Depositing dielectric material into a void or trench yields an optical waveguide integrated within the front-end of the wafer. For example, a shallow trench isolation (STI) layer formed in-foundry may serve as a high-resolution patterning waveguide template in a damascene process within the front end of a die or wafer.

Abstract: Various aspects described herein relate to electrochemical devices, e.g., for separation of one or more target organic or inorganic molecules (e.g., charged or neutral molecules) from solution, and methods of using the same. In particular embodiments, the electrochemical devices and methods described herein involve at least one redox-functionalized electrode, wherein the electrode comprises an immobilized redox-species that is selective toward a target molecule (e.g., charged molecule such as ion or neutral molecule). The selectivity is based on a Faradaic/redox-activated chemical interaction (e.g., directional hydrogen binding) between the oxidized state of the redox species and a moiety of the target molecule (e.g., charged molecule such as ion or neutral molecule).

Abstract: Metal bioremediation and metal mining strategies can include compositions and methods.