Abstract: An improved waveguide is disclosed. The waveguide utilizes a luminescent material disposed within or around its perimeter to introduce additional light into the waveguide. For example, the waveguide may include a plurality of planar layers having different refractive indexes. A luminescent material may be disposed along the outer edge of these layers. When light from within the waveguide strikes the luminescent material, it emits light, thereby adding to the light in the waveguide. Not only does the luminescent material introduce more light into the waveguide, it also introduces more light sources, thereby making it more difficult to introduce a probe without blocking at least a portion of the light destined for the image sensor. The luminescent material may be a phosphor.

Abstract: Heat exchange structure. A hydrophilic, thermally conductive porous medium includes nanostructures formed substantially uniformly throughout the porous medium providing a balance of capillary and viscous forces to self-regulate a liquid-vapor contact line. A suitable porous medium is copper. A method for making the structure is also disclosed.

Abstract: Methods for treating, adhering, or sealing biological tissue are provided. The methods include combining solutions containing a polymer component and a dendrimer component capable of reacting with each other, and at least one of the components includes a substituent capable of photoreversible dimerization that can be reversibly dimerized. Drug delivery compositions and kits containing these two components also are provided.

Abstract: One or more input/output devices accept user-inputted path instructions that specify a set of multiple deposition paths for an extruder to travel. An actuator actuates motion of the extruder along a trajectory that includes each of the deposition paths and also includes multiple non-deposition paths. For each deposition path: (a) the user-inputted path instructions specify a thickness of an object; and (b) the extruder extrudes the object in accordance with fabrication instructions computed by a computer based at least in part on the thickness. As the extruder moves over the entire trajectory, the extruder extrudes a set of objects, one object per deposition path. The objects adhere to each other to form an integral 3D structure. In some cases, the objects include functionally graded material.

Abstract: The invention provides for systems, methods, and compositions for targeting and editing nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a RNA-targeting Cas13 protein, at least one guide molecule, and at least one cytidine deaminase protein or catalytic domain thereof.

Abstract: Self-righting articles, such as self-righting capsules for administration to a subject, are generally provided. In some embodiments, the self-righting article may be configured such that the article may orient itself relative to a surface (e.g., a surface of a tissue of a subject). The self-righting articles described herein may comprise one or more tissue engaging surfaces configured to engage (e.g., interface with, inject into, anchor) with a surface (e.g., a surface of a tissue of a subject). In some embodiments, the self-righting article may have a particular shape and/or distribution of density (or mass) which, for example, enables the self-righting behavior of the article. In some embodiments, the self-righting article may comprise a tissue interfacing component and/or a pharmaceutical agent (e.g., for delivery of the active pharmaceutical agent to a location internal of the subject) such as a liquid pharmaceutical agent.

Abstract: Chromatography systems and methods for using carbon dioxide to separate one or more cannabis-derived compounds from other components of a mixture are generally described. Some of the methods described herein comprise transporting a mixture comprising a first cannabis-derived compound and one or more other components through a chromatography column containing a stationary phase comprising a packing material. In some embodiments, the mixture is transported through the column within a mobile phase that comprises carbon dioxide (e.g., supercritical CO2, liquid CO2). The mobile phase may be substantially free of a co-solvent that is in liquid phase at standard room temperature and pressure. In some embodiments, the mobile phase is free of any co-solvent and comprises 100 vol % carbon dioxide.

Abstract: Provided herein are methods of detecting a nucleic acid modification, methods for detecting off-target activity of a targeted nuclease specific for a selected target sequence, methods for determining cleavage efficiency of a targeted nuclease specific for a selected target sequence, methods for selecting a guide RNA from a plurality of guide RNAs specific for a selected target sequence, methods for enrichment of one or more nucleic acid molecules wherein a nucleic acid modification is made and kits of parts for use in such methods.

Abstract: Systems and methods for generating and evaluating candidate sequences of partitioning steps to partition at least one biologically produced product from at least one impurity. In some embodiments, a plurality of candidate sequences of partitioning steps may be generated, wherein at least one candidate sequence of the plurality of candidate sequences comprises a plurality of partitioning steps in a specified order. The plurality of candidate sequences may be evaluated. For instance, a data set associated with the at least one partitioning step may be accessed, the data set comprising: first data indicative of a behavior of the at least one biologically produced product with respect to the at least one partitioning step; and second data indicative of a behavior of the at least one impurity with respect to the at least one partitioning step. The at least one candidate sequence may be scored based at least in part on the data set.

Abstract: A system includes a camera positioned in an environment to capture image data of a subject; a computing device communicatively coupled to the camera, the computing device comprising a processor and a non-transitory computer-readable memory; and a machine-readable instruction set stored in the non-transitory computer-readable memory. The machine-readable instruction set causes the computing device to perform at least the following when executed by the processor: receive the image data from the camera; analyze the image data captured by the camera using a neural network trained on training data generated from a 360-degree panoramic camera configured to collect image data of a subject and a visual target that is moved about an environment; and predict a gaze direction vector of the subject with the neural network.

Abstract: In some aspects, the disclosure relates to compositions and method for detection, classification, and treatment of prostate cancer. In some embodiments, the disclosure relates to prostate protease nanosensors comprising a scaffold linked to a prostate-specific substrate that include a detectable marker capable of being released from the prostate protease nanosensor when exposed to an enzyme present in a prostate. In some embodiments, the disclosure relates to methods of classifying prostate cancer in a subject based upon detection of detectable markers in a sample obtained from a subject who has been administered prostate protease nanosensors.

Abstract: The present invention relates to composition comprising at least one non-pathogenic bacterial cell, wherein the non-pathogenic bacterial cell comprises at least a first and a second nucleic acid sequence, the first nucleic acid sequence comprising at least one non-constitutive promoter operably linked to the second nucleic acid sequence that encodes therapeutic agent, wherein the non-constitutive promoter is an inducible promoter responsive to at least one stimuli and the at least one stimuli comprises the presence of a certain density or a certain number of bacterial cells comprising the first and second nucleic acid sequences.

Abstract: A system for generating an image of a region of interest (ROI) of a target object, the system including a camera, a target stage configured to receive the target object, the target stage configured to provide a translational movement and a rotational movement of the target object, and a controller. The controller is configured to control the camera and target stage to iteratively shift the target along scan trajectories of sample locations to capture images of each of a plurality of concentric rings and sub-rings of a predefined radial pitch over the ROI, the sample locations represented by polar coordinates defining sectors of each of the sub-rings. The controller is further configured to extract super resolution (SR) pixels from the images to reconstruct an SR image of each of the rings in the polar coordinates, and project the SR images into Cartesian coordinate images.

Abstract: A non-paraxial Talbot spectrometer includes a transmission grating to receive incident light. The grating period of the transmission grating is comparable to the wavelength of interest so as to allow the Talbot spectrometer to operate outside the paraxial limit. Light transmitted through the transmission grating forms periodic Talbot images. A tilted detector is employed to simultaneously sample the Talbot images at various distances along a direction perpendicular to the grating. Spectral information of the incident light can be calculated by taking Fourier transform of the measured Talbot images or by comparing the measured Talbot images with a library of intensity patterns acquired with light sources having known wavelengths.

Abstract: The disclosure provides novel large serine recombinases and their respective recognition sites, as well as libraries of orthogonal recombinase recognition sites. Uses of the large serine recombinases, recognition sites, and libraries of orthogonal recombinase recognition sites also are provided.

Abstract: Covalently modified alginate polymers, possessing enhanced biocompatibility and tailored physiochemical properties, as well as methods of making and use thereof, are disclosed herein. The covalently modified alginates are useful as a matrix for coating of any material where reduced fibrosis is desired, such as encapsulated cells for transplantation and medical devices implanted or used in the body.

Abstract: A two-layer erasure-coded fault-tolerant distributed storage system offering atomic access for read and write operations is described. In some embodiments, a class of erasure codes known as regenerating codes (e.g. minimum bandwidth regenerating codes) for storage of data in a backend layer is used to reduce a cost of backend bulk storage and helps in reducing communication cost of read operations, when a value needs to be recreated from persistent storage in the backend layer. By separating the functionality of edge layer servers and backend servers, a modular implementation for atomicity using storage-efficient erasure-codes is provided. Such a two-layer modular architecture permits protocols needed for consistency implementation to be substantially limited to the interaction between clients and an edge layer, while protocols needed to implement erasure code are substantially limited to interaction between edge and backend layers.

Abstract: Zwitterionic polymers or biocompatible polymers with improved properties for cell encapsulation, coating of devices, or a combination thereof are described. The biocompatible polymer contains a zwitterionic monomer, a monomer with a reactive side chain, and optionally another hydrophobic monomer or a neutral hydrophilic monomer. The zwitterionic polymers are cross-linked with a cross-linker via covalent bond to form a zwitterionic hydrogel in the presence of cells. Also provided, are methods of making and using the zwitterionic polymers.

Abstract: A circuit for secure operation includes a plurality of mutually exclusive circuit zones including a first circuit zone having a first level of security and a second circuit zone having a second level of security less than the first level of security and one or more gate circuits each providing limited transfer of data between the circuit zones, the gate circuits providing all data connectivity between the first circuit zone and the second circuit zone and statically configured to prevent unmodified transfer of data from the first circuit zone to the second circuit zone.

Abstract: Metal-sulfur energy storage devices also comprising new redox mediator compounds are described.