Abstract: The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.
Type:
Grant
Filed:
April 18, 2007
Date of Patent:
May 1, 2012
Assignee:
Metabolic Explorer
Inventors:
Philippe Soucaille, Rainer Figge, Christian Croux
Abstract: The present invention claims an isolated polypeptide having L-amino-acid-N-acyl transferase enzymatic activity and a modified microorganism in which this enzyme is overexpressed. Substrates of said enzyme include mainly methionine and their derivatives or analogs. Overexpression in sulphur-containing amino acid producing microorganisms permits the production of large amounts of N-acylated sulphur-containing amino acids. The isolation of the N-acylated sulphur-containing amino acids from the fermentation medium is also claimed.
Abstract: The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a microorganism on a culture medium with high glycerine content. The invention also concerns a new microorganism, or strain of microorganism, adapted for the production of 1,3-propanediol from medium comprising high glycerine content. The invention also concerns an “adapted microorganism” which glycerol metabolism is directed to 1,3-propanediol production, and which is allowed to grow in the presence of a high concentration of industrial glycerine. The invention also concerns a biosourced 1,3-propanediol obtained by the process thereof. Finally the invention concerns the use of the above described biosourced 1,3-propanediol as extender chain in thermoplastic polyurethane, as monomers in polytrimethylene terephtalate and as a component in cosmetics formulations.
Type:
Application
Filed:
May 5, 2010
Publication date:
March 8, 2012
Applicant:
METABOLIC EXPLORER
Inventors:
Michel Chateau, Jean-Yves Dubois, Philippe Soucaille
Abstract: The invention relates to optimised micro-organism strains for the biotransformation production of molecules having NADPH-consuming biosynthetic pathways. The inventive strains can be used in NADPH-consuming biotransformation methods. Said strains are characterized in that one or more NADPH-oxidizing activities are limited.
Type:
Grant
Filed:
November 5, 2004
Date of Patent:
January 3, 2012
Assignee:
Metabolic Explorer
Inventors:
Gwénaëlle Bestel-Corre, Cédric Boisart, Michel Chateau, Benjamin Gonzalez, Philippe Soucaille, Rainer Figge, Olivier Zink
Abstract: The present invention concerns a new method for the biological preparation of a diol comprising culturing a microorganism genetically modified for the bioproduction of an aliphatic diol, wherein the microorganism comprises a metabolic pathway for the decarboxylation of a hydroxy-2-keto-aliphatic acid metabolite with an enzyme having a 2-keto acid decarboxylase activity, the product obtained from said decarboxylation step being further reduced into the corresponding aliphatic diol, and wherein the microorganism is genetically modified for the improved production of said hydroxy-2-keto-aliphatic acid metabolite. The invention also concerns a modified microorganism for the production of an aliphatic diol.
Abstract: Method for purifying an alcohol from a fermentation broth comprising adding a heavy solvent, evaporating the alcohol to be purified, and mechanically drawing out of the crystallized salts by heavy solvent flow and scrapping on a falling film evaporator, a wiped film evaporator, a thin film evaporator or a short path evaporator.
Abstract: The present invention is relative to a method for producing 1,2-propanediol by fermentation, comprising: cultivating a microorganism producing 1,2-propanediol in an appropriate medium comprising a source of sucrose, and recovering the 1,2-propanediol being produced, wherein the microorganism is able to utilize sucrose as sole carbon source for the production of 1,2-propanediol. In a preferred aspect of the invention, the source of sucrose is obtained from plant biomass, and is in particular sugar cane juice.
Type:
Application
Filed:
November 7, 2008
Publication date:
September 8, 2011
Applicant:
METABOLIC EXPLORER
Inventors:
Francois Voelker, Rainer Figge, Philippe Soucaille
Abstract: The present invention concerns a modified microorganism with an increased methylglyoxal reductase activity, and its use for the preparation of 1,2-propanediol and/or acetol. In particular this increased methylglyoxal reductase activity is obtained by increasing the expression of specific genes from microorganisms. This invention is also related to a method for producing 1,2-propanediol and/or acetol by fermentation of a microorganism having an increased methylglyoxal reductase activity.
Type:
Application
Filed:
March 21, 2008
Publication date:
August 18, 2011
Applicant:
METABOLIC EXPLORER
Inventors:
Philippe Soucaille, Salles Isabelle Meynial, Francois Voelker, Rainer Figge
Abstract: The present invention concerns a new method for the biological preparation of 2-hydroxy-isobutyrate (2-HIBA), including a fermentation method with microorganisms modified to favour production of 2-HIBA from renewable resources. The invention also concerns the modified microorganisms used in such fermentation method.
Abstract: The present invention relates to a novel polypeptide having the enzymatic activity of conversion of methylglyoxal to lactic acid in a single step (known as glyoxalase III activity), a polynucleotide having a nucleotide sequence encoding such polypeptide and uses thereof. The invention relates to the modulation of the glyoxalase III activity in a microorganism by varying the expression level of the polynucleotide coding for such polypeptide. The invention also relates to the production of commodity chemicals, especially 1,2-propanediol, acetol, and lactic acid by fermenting microorganisms wherein their glyoxalase III activity is modulated.
Abstract: The present invention provides a method for the biological production of n-butanol at high yield from a fermentable carbon source. In one aspect of the present invention, a process for the conversion of glucose to n-butanol is achieved by the use of a recombinant organism comprising a host C. acetobutylicum transformed i) to eliminate the acetate pathway ii) to eliminate the butyrate pathway iii) to eliminate the lactate pathway and iv) to eliminate the acetone pathway. In another aspect of the present invention, the hydrogen flux is decreased and the reducing power redirected to n-butanol production by interrupting the expression of the hydrogenase gene. Optionally the n-butanol produced can be eliminated during the fermentation by gas striping and further purified by distillation.
Abstract: The present invention is related to a new method for interrupting multiple DNA sequences in Clostridia, even in genes recognized to be essential for the optimal growth of Clostridii by using a counter-selectable marker that would pinpoint the cells that have lost the plasmid and acquired a modified function that permits survival without the interrupted gene. This method is easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.
Abstract: This invention concerns a microorganism useful for the production of acetol from a simple carbon source, wherein said microorganism is characterized by: an improved activity of the biosynthesis pathway from dihydroxyacetone phosphate to acetol, and an attenuated activity of the glyceraldehyde 3-phosphate dehydrogenase This invention also concerns a method for producing acetol by fermentating a microorganism according to the invention.
Type:
Application
Filed:
March 21, 2008
Publication date:
November 4, 2010
Applicant:
Metabolic Explorer
Inventors:
Philippe Soucaille, Francois Voelker, Rainer Figge
Abstract: Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.
Abstract: The present invention is directed to a method for the preparation of evolved microorganisms permitting the creation or modification of metabolic pathways. Strains of evolved microorganisms and evolved genes obtained by the method are also provided. The invention is also directed to the use of evolved microorganisms, genes or proteins in a biotransformation process.
Type:
Grant
Filed:
February 18, 2004
Date of Patent:
June 29, 2010
Assignee:
Metabolic Explorer
Inventors:
Michel Chateau, Benjamin Gonzalez, Isabelle Meynial-Salles, Philippe Noel Paul Soucaille, Olivier Zink
Abstract: The present invention provides a method for the anaerobic production of 1,3 propanediol, by culturing a Clostridium strain in an appropriate culture medium comprising glycerol as a source of carbon, wherein said Clostridium strain does not produce substantially other products of the glycerol metabolism selected among the group consisting of: butyrate, lactate, butanol and ethanol, and recovering of 1,3-propanediol.
Abstract: The present invention provides a method for the biological production of n-butanol at high yield from a fermentable carbon source. In one aspect of the present invention, a process for the conversion of glucose to n-butanol is achieved by the use of a recombinant organism comprising a host C. acetobutilicum transformed i) to eliminate the butyrate pathway ii) to eliminate the acetone pathway iii) to eliminate the lactate pathway and iv) to eliminate the acetate pathway. In another aspect of the present invention, the hydrogen flux is decreased and the reducing power redirected to n-butanol production by attenuating the expression of the hydrogenase gene. Optionally the n-butanol produced can be eliminated during the fermentation by gas striping and further purified by distillation.
Abstract: The present invention relates to the use of recombinant homoserine transsuccinylase enzymes with altered feedback sensitivity (MetA*) and possibly recombinant S-adenosyl methionine synthetase enzymes with reduced activity (MetK*) for the production of methionine, its precursors or derivatives thereof.
Type:
Application
Filed:
May 12, 2005
Publication date:
January 29, 2009
Applicant:
Metabolic Explorer
Inventors:
Gwenaelle Bestel-Corre, Michel Chateau, Rainer Figge, Celine Raynaud, Philippe Soucaille