Abstract: A method for characterizing a sample containing at least one compound, preferably a biological sample comprising at least one metabolite, is proposed. In a first process step, a three-dimensional first set of data is generated by analyzing the sample by using at least one time resolved separation technique (214) and at least mass one resolved separation technique (216). The set of data comprises at least one signal I as a function of a mass variable over a first range of measurement (420) and of a time variable over a second range of measurement (422). In a second process step, the first range of measurement (420) is divided into at least two mass variable intervals (424). For each mass variable interval (424), an extracted signal is selected, wherein the extracted signal is a function of the time variable. In a third process step, the second range of measurement (422) is divided into at least one time variable interval (426).
Abstract: The present invention relates to a method for analyzing at least one test sample, wherein said test sample comprises at least one compound, said method comprising the steps of: a) providing at least one test sample comprising at least one compound; b) determining said at least one compound in said test sample whereby raw results are generated; and c) analyzing the raw results obtained in step b), wherein the analysis of said at least one test sample is accompanied by an analysis of at least one reference sample; and wherein the test sample and the reference sample are analyzed in an identical sequence in each step of the method.
Type:
Application
Filed:
July 25, 2006
Publication date:
September 25, 2008
Applicant:
METANOMICS GMBH
Inventors:
Tilmann B. Walk, Ralf Looser, Bianca Bethan, Michael Manfred Herold, Beate Kamlage, Oliver Schmitz, Jan C Wiemer, Alexandre Prokoudine, Bennard van Ravenzwaay, Werner Mellert, Georgia Coelho Palermo Cunba, Thomas Ehrhardt, Gerhard Krennrich
Abstract: The present invention relates to a process for the increase in yield in a plant organisms by reducing gene expression. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material and plants.
Abstract: The invention relates to a method and device for analyzing a sample containing a number of substances. The method comprises the following steps: recording one or more mass spectra (mass spectra data D) of the sample (P); deconvoluting the mass spectra in a first evaluating device (A), which assigns the chromatographic peaks (a) and their associated deconvoluted mass spectra of one substance at a time based on the correspondence with a reference spectrum of the substance, and/or determining the intensity of the obtained peaks of the ions of the mass spectra in an evaluating device (B), which assigns the chromatographic peaks (b) of the ions and their associated mass spectra based on the correspondence of selective ions and their retention time ranges of the peaks (b) with the reference values of the substance predetermined for a substance, and verifying the assignments in a validating device (V).
Type:
Application
Filed:
February 1, 2006
Publication date:
June 5, 2008
Applicant:
Metanomics GmbH
Inventors:
Michael Manfred Herold, Martin Kluttig, Nicole Christiansen, Joachim Kopka, Jorn Quedenau
Abstract: The present invention relates to a process for isolating constituents from organic material, comprising the following process steps: a) freeze-drying of the organic material; b) extracting the constituents with a polar solvent or solvent mixture (A) and an organic solvent or solvent mixture (B), it being possible for the extracts of the extraction with (A) and (B) to form one phase; c) combining the extracts (A) and (B) to give one phase; and d) carrying out an esterification/transesterification in the unpolar phase with an alcohol, the esterification/transesterification being carried out in the presence of a volatile acid; the process being a high-throughput process.
Type:
Grant
Filed:
November 11, 2002
Date of Patent:
December 25, 2007
Assignee:
Metanomics GmbH & Co. KGaA
Inventors:
Michael Manfred Herold, Martin Dostler, Ralf Looser, Tilmann B. Walk, Achim Fegert, Martin Kluttig, Britta Lehmann, Silke Heidemann, Annette Hennig, Joachim Kopka
Abstract: The present invention relates to a process for the production of the fine chemical in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof, preferably in plastids. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.
Type:
Application
Filed:
September 6, 2006
Publication date:
May 24, 2007
Applicant:
Metanomics GmbH
Inventors:
Piotr Puzio, Birgit Wendel, Michael Herold, Ralf Looser, Astrid Blau, Gunnar Plesch, Beate Kamlage, Florian Schauwecker
Abstract: The invention relates to a mass spectrometry method for analysing mixtures of substances using a triple quadrupole mass spectrometer, whereby said mixtures of substances are ionised prior to analysis.
Abstract: The present invention relates to a method of identifying herbicidally active compounds. The invention furthermore relates to nucleic acid constructs, to vectors comprising the nucleic acid constructs, to transgenic organisms and to their use. Moreover, the present invention relates to substances which have been identified using the abovementioned method.