Abstract: A synthesis method for a second strand in DNB paired-end sequencing, a sequencing method and a related product. The synthesis method for the second strand comprises: after a first-strand sequencing on the DNB is completed, performing a second-strand synthesis using a second-strand synthesis primer, wherein the second-strand synthesis primer is a primer capable of being anchored on the surface of a sequencing chip.

Abstract: An automated library preparation system (1) includes a consumable item storage module (10) for storing a consumable item; a dispatching transfer module (30) capable of moving along a predetermined direction; a biochemical reaction module (20) including a plurality of biochemical reaction devices (21a, 21b) which are arranged in turn along the predetermined direction, each of the plurality of biochemical reaction devices being configured to perform one subprocess of sample preparation for gene sequencing, the dispatching transfer module (30) being configured to grab the consumable item (2) stored in the consumable item storage module, the dispatching transfer module being further configured to transfer the consumable item to the plurality of biochemical reaction devices when sliding along the predetermined direction; and a control module configured to control the consumable item storage module, the biochemical reaction module, and the dispatching transfer module to work collaboratively.

Abstract: The present disclosure relates to the technical field of sequencing, and in particular to a method and reagent for preparing a sequencing library. Provided in the present disclosure is a method for preparing a sequencing library. According to the method, by means of further adding an additive or an additive combination capable of reducing the DNA secondary structure and/or reducing the degradation of single-stranded DNA in the single-chain circularization reaction and/or the rolling circle amplification reaction, and further adding an additive or an additive combination capable of reducing the DNA secondary structure and reducing the degradation of single-stranded DNA and/or reducing the stability of the double helix structure of double-stranded DNA in the rolling circle amplification reaction of the double-stranded circular library, the ability of the DNA ligase and/or the strand displacement DNA polymerase to bind to a target nucleic acid is enhanced.

Abstract: This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.

Abstract: A photodiode (112, 200, 400, 500) includes a semiconductor substrate (210) having a first surface (211) and a second surface (212, 412, 512), and a light sensing junction (220) located adjacent to the first surface (211). The second surface (212, 412, 512) is located opposite the first surface (211), and the second surface (212, 412, 512) includes a concave surface covering a recessed region (415, 515) in the semiconductor substrate (210).

Abstract: Provided is a DNA polymerase mutant for sequencing. The DNA polymerase mutant includes: compared with a Pyrococcus abyssi DNA polymerase exo-mutant, at least three amino acid mutations in the following four sites or functionally equivalent sites: position 409, position 410, position 411, and position 486. The Pyrococcus abyssi DNA polymerase exo-mutant has an amino acid sequence as set forth in SEQ ID NO: 1.

Abstract: Systems and methods for immersion imaging of a flowcell. In one example, an imaging system includes liquid ports in leading and trailing positions relative to a scanned imaging objective. The leading liquid port dispenses an immersion liquid into a space between the distal end of the imaging objective and the flowcell's cover, with the trailing liquid port collecting the immersion liquid. In another example, an imaging system includes a flowcell with a cover that is moveable relative a substrate. The system is configured to move the imaging objective and flowcell cover as a unit relative to the flowcell substrate.

Abstract: Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

Abstract: Provided is a synchronous sequencing method, including: constructing a sequencing library for a nucleic acid sample to be tested; loading the sequencing library onto a sequencing chip; performing a plurality of synchronous sequencing reaction cycles on the sequencing library, wherein an image set generated in each of the plurality of synchronous sequencing reaction cycles constitutes a raw image set of the synchronous sequencing; acquiring a base-calling result of the synchronous sequencing based on the raw image set of the synchronous sequencing; correcting the signal intensity value of each base channel based on a predetermined correction parameter to obtain a corrected base-calling result; and determining a base output result of the synchronous sequencing based on the corrected base-calling result.

Abstract: Embodiments of the disclosure include methods and systems for nucleic acid sequencing that may include an objective coupled to an actuator, wherein the actuator is configured to move the objective over a surface of a substrate. In some embodiments, a droplet may be disposed on the surface of the substrate, and the droplet may be moved along with the objective. The distal end of the objective may include a material that provides a higher friction against the droplet than a material of the surface of the substrate. In some embodiments, the distal end of the objective may be immersed in a fluid as it is moved over the surface of the substrate. The substrate may include vertical walls within a region to retain the fluid.

Abstract: Methods and compositions for preparing a nucleic acid sequencing library are described including (a) transposing an insertion sequence into first fragments of the target nucleic acid, wherein the insertion sequence comprises a hybridization sequence, and wherein the transposing produces nicks in the first fragments; (b) combining in a single mixture (i) the first fragments of the target nucleic acid from (a), (ii) a splint oligonucleotide, and (iii) a population of beads, wherein each bead comprises capture oligonucleotides immobilized thereon, and (c) ligating capture oligonucleotides of individual beads to inserted hybridization sequences of individual first fragments.

Abstract: This application relates to methods and compositions used for paired-end sequencing. The method comprises providing a DNA array comprising a surface immobilized with DNA concatemers. For each of the plurality of DNA concatemers on the array, the method comprises annealing first read primers to primer binding sites on the DNA concatemer, extending at least some of the first read primers to incorporate dNTPs or dNTP analogs, thereby producing first read strands. Each of the dNTPs or dNTP analogs being incorporated is identified to produce first reads, performing controlled MDA by extending at least some of the first read strands with a polymerase having strand-displacement activity to generate a plurality of partially hybridized second strands. Second read primers are then annealed to the single-stranded branches of the plurality of second strands to generate the second reads.

Abstract: Provided are a reagent kit and use thereof in sequencing. The reagent kit includes a first reagent and a second reagent. The first reagent is selected from a disulfide bond reducing agent. The second reagent is selected from a blocking reagent suited to blocking a disulfide bond.

Abstract: A method for analyzing droplets based on images: obtaining a working dye channel image and a reference dye channel image corresponding to a droplet system (S1); separately performing a preset image processing operation on the working dye channel image and the reference dye channel image (S2); using the working dye channel image or the reference dye channel image as a target image (S3), and determining a region in which each droplet is located from within the target image (S4); and on the basis of the determined region in which each droplet is located, analyzing the droplet system (S5). The method can effectively improve the accuracy of droplet analysis. Further provided are a computer device and a storage medium.

Abstract: An apparatus for forming a plurality of microdroplets (26a, 26?, 56) from a droplet (16, 26, 263) includes a substrate (12, 22, 22b, 22c, 1101), a dielectric layer (13, 23, 23b, 23c, 43a, 43b, 43) on the substrate (12, 22, 22b, 22c, 1101) and having a plurality of hydrophilic surface regions (48a, 491, 49) spaced apart from each other by a hydrophobic surface (44, 46), and a plurality of electrodes (14, 14a, 14b, 24, 24a, 24b, 24c, 34a, 34b, 34c) in the dielectric layer (13, 23, 23b, 23c, 43a, 43b, 43).

Abstract: A fluid transportation system and method use a negative pressure driving mode to absorb fluids from a fluid storage assembly and a positive pressure driving mode to load the absorbed fluids into the fluid-using system. At least two working modules are used to transport fluids to the fluid-using system, the process of transporting fluid in each working module being divided into the processes of absorbing fluids into a fluid transfer assembly, and loading fluids into a fluid transfer assembly. The process of absorbing fluids in each working module and the processes of absorbing fluids and/or loading fluids in any other working module overlap at least partially along the time axis. With the present disclosure, the accuracy and speed of fluids transportation are improved, a device utilizing the system and method is also disclosed.

Abstract: A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.

Abstract: An optical imaging system (1) is configured for photographing a sample and includes a lighting module (11) and an imaging module (15). The lighting module (11) is configured for outputting excitation light, the excitation light is configured to excite the sample to generate excited light, the imaging module (15) comprises a time delay integration line scan camera (151), the time delay integration line scan camera (151) is configured to record the excited light. A biochemical substance detection system using the optical imaging system (1) is also provided, improving the detection flux.

Abstract: An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.