Abstract: A nucleic acid sequencing system may include a substrate coupled to a rotating disk. The substrate may include a plurality of nucleic acid samples. A detection system, including for example an objective and a camera, may detect sequencing events on the substrate while the substrate is rotated relative to the detection system around a rotational axis of the substrate, perpendicular to a surface of the substrate, by the actuation system.

Abstract: A biochemical substance analysis system is used to detect biological characteristics of a sample in a flow cell, and includes a detection system, a scheduling system, a biochemical reaction system, and a control system. The scheduling system is used to schedule the flow cell at different sites, including sites in the detection system and sites in the biochemical reaction system. The biochemical reaction system is used to allow the sample to react in the flow cell. The detection system is used to detect a signal from the reacted sample to obtain a signal representing the biological characteristics of the sample. The control system is used to control the detection system, the scheduling system, and the biochemical reaction system to cooperate. The present disclosure improves the automation degree and flux of the biochemical substance analysis.

Abstract: Provided are a method for constructing a sequencing library based on a DNA sample and use. The method includes: digesting the DNA sample with endonuclease to obtain a DNA sample with single-strand nicks; polymerizing the DNA sample with the single-strand nicks by using polymerase, dATP, dTTP, dGTP, and methylated dCTP to obtain a hybrid DNA, the hybrid DNA including two reversely complementary strands, where a 5?-end of each strand is an original sequence of the DNA sample, a 3?-end of each strand is a synthetic sequence, and all bases C in the 3?-end of each strand are methylated; subjecting the hybrid DNA to bisulfite treatment or other treatment to obtain converted hybrid DNA; and amplifying the converted hybrid DNA to obtain the sequencing library. Thus, the method can be used for whole genome bisulfite sequencing or multiplex PCR targeted sequencing and probe capture sequencing.

Abstract: Provided are a mask, a sample collecting tube, and a pathogen collecting apparatus. The mask includes a mask body, a breather valve fixed on the mask body, and a sampling structure including a pathogen adsorption portion. The pathogen adsorption portion is disposed on an inner side of the breather valve and is adapted to adsorb pathogens in exhaled gas. The pathogen adsorption portion is adapted to enter the sample collecting tube to be in contact with a sample preservation solution in the sample collecting tube.

Abstract: A method and system for constructing sequencing library, includes: obtaining a transformed DNA sample with a universal sequence; performing amplification using a first specific primer located upstream of the target region and a first universal primer at least partially matching or overlapping the universal sequence; and performing amplification using a second specific primer, a second universal primer and a tagged primer. The second specific primer is located downstream of the first specific primer and upstream of the target region, the second universal primer overlaps at least a partial sequence of the second specific primer, and the tagged primer overlaps a partial sequence of the first universal primer. Alternatively, the second specific primer is located downstream of the target region, the second universal primer overlaps at least a partial sequence of the first specific primer, and the tagged primer overlaps a partial sequence of the second specific primer.

Abstract: A field-of-view stitching system including: a light source module for emitting a first beam; a light guiding element for splitting the first beam into a plurality of second beams and includes an incident surface, a first exit surface, and a plurality of second exit surfaces, the second beams propagate along an optical path of the first beam; and a plurality of light modulators, a number of the plurality of light modulators is the same as a total number of the first exit surface and the plurality of second exit surfaces, each of the plurality of light modulators is configured to receive and regularly reflect one of the plurality of second beams; the light guiding element is further configured to receive and combine the second beams reflected by the light modulators to form an illuminating light. A field-of-view stitching method, a biological sample identification device and method are also provided.

Abstract: A synthesis method for a second strand in DNB paired-end sequencing, a sequencing method and a related product. The synthesis method for the second strand comprises: after a first-strand sequencing on the DNB is completed, performing a second-strand synthesis using a second-strand synthesis primer, wherein the second-strand synthesis primer is a primer capable of being anchored on the surface of a sequencing chip.

Abstract: An automated library preparation system (1) includes a consumable item storage module (10) for storing a consumable item; a dispatching transfer module (30) capable of moving along a predetermined direction; a biochemical reaction module (20) including a plurality of biochemical reaction devices (21a, 21b) which are arranged in turn along the predetermined direction, each of the plurality of biochemical reaction devices being configured to perform one subprocess of sample preparation for gene sequencing, the dispatching transfer module (30) being configured to grab the consumable item (2) stored in the consumable item storage module, the dispatching transfer module being further configured to transfer the consumable item to the plurality of biochemical reaction devices when sliding along the predetermined direction; and a control module configured to control the consumable item storage module, the biochemical reaction module, and the dispatching transfer module to work collaboratively.

Abstract: The present disclosure relates to the technical field of sequencing, and in particular to a method and reagent for preparing a sequencing library. Provided in the present disclosure is a method for preparing a sequencing library. According to the method, by means of further adding an additive or an additive combination capable of reducing the DNA secondary structure and/or reducing the degradation of single-stranded DNA in the single-chain circularization reaction and/or the rolling circle amplification reaction, and further adding an additive or an additive combination capable of reducing the DNA secondary structure and reducing the degradation of single-stranded DNA and/or reducing the stability of the double helix structure of double-stranded DNA in the rolling circle amplification reaction of the double-stranded circular library, the ability of the DNA ligase and/or the strand displacement DNA polymerase to bind to a target nucleic acid is enhanced.

Abstract: This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.

Abstract: A photodiode (112, 200, 400, 500) includes a semiconductor substrate (210) having a first surface (211) and a second surface (212, 412, 512), and a light sensing junction (220) located adjacent to the first surface (211). The second surface (212, 412, 512) is located opposite the first surface (211), and the second surface (212, 412, 512) includes a concave surface covering a recessed region (415, 515) in the semiconductor substrate (210).

Abstract: Provided is a DNA polymerase mutant for sequencing. The DNA polymerase mutant includes: compared with a Pyrococcus abyssi DNA polymerase exo-mutant, at least three amino acid mutations in the following four sites or functionally equivalent sites: position 409, position 410, position 411, and position 486. The Pyrococcus abyssi DNA polymerase exo-mutant has an amino acid sequence as set forth in SEQ ID NO: 1.

Abstract: Systems and methods for immersion imaging of a flowcell. In one example, an imaging system includes liquid ports in leading and trailing positions relative to a scanned imaging objective. The leading liquid port dispenses an immersion liquid into a space between the distal end of the imaging objective and the flowcell's cover, with the trailing liquid port collecting the immersion liquid. In another example, an imaging system includes a flowcell with a cover that is moveable relative a substrate. The system is configured to move the imaging objective and flowcell cover as a unit relative to the flowcell substrate.

Abstract: Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

Abstract: Provided is a synchronous sequencing method, including: constructing a sequencing library for a nucleic acid sample to be tested; loading the sequencing library onto a sequencing chip; performing a plurality of synchronous sequencing reaction cycles on the sequencing library, wherein an image set generated in each of the plurality of synchronous sequencing reaction cycles constitutes a raw image set of the synchronous sequencing; acquiring a base-calling result of the synchronous sequencing based on the raw image set of the synchronous sequencing; correcting the signal intensity value of each base channel based on a predetermined correction parameter to obtain a corrected base-calling result; and determining a base output result of the synchronous sequencing based on the corrected base-calling result.

Abstract: Embodiments of the disclosure include methods and systems for nucleic acid sequencing that may include an objective coupled to an actuator, wherein the actuator is configured to move the objective over a surface of a substrate. In some embodiments, a droplet may be disposed on the surface of the substrate, and the droplet may be moved along with the objective. The distal end of the objective may include a material that provides a higher friction against the droplet than a material of the surface of the substrate. In some embodiments, the distal end of the objective may be immersed in a fluid as it is moved over the surface of the substrate. The substrate may include vertical walls within a region to retain the fluid.

Abstract: Methods and compositions for preparing a nucleic acid sequencing library are described including (a) transposing an insertion sequence into first fragments of the target nucleic acid, wherein the insertion sequence comprises a hybridization sequence, and wherein the transposing produces nicks in the first fragments; (b) combining in a single mixture (i) the first fragments of the target nucleic acid from (a), (ii) a splint oligonucleotide, and (iii) a population of beads, wherein each bead comprises capture oligonucleotides immobilized thereon, and (c) ligating capture oligonucleotides of individual beads to inserted hybridization sequences of individual first fragments.

Abstract: This application relates to methods and compositions used for paired-end sequencing. The method comprises providing a DNA array comprising a surface immobilized with DNA concatemers. For each of the plurality of DNA concatemers on the array, the method comprises annealing first read primers to primer binding sites on the DNA concatemer, extending at least some of the first read primers to incorporate dNTPs or dNTP analogs, thereby producing first read strands. Each of the dNTPs or dNTP analogs being incorporated is identified to produce first reads, performing controlled MDA by extending at least some of the first read strands with a polymerase having strand-displacement activity to generate a plurality of partially hybridized second strands. Second read primers are then annealed to the single-stranded branches of the plurality of second strands to generate the second reads.

Abstract: Provided are a reagent kit and use thereof in sequencing. The reagent kit includes a first reagent and a second reagent. The first reagent is selected from a disulfide bond reducing agent. The second reagent is selected from a blocking reagent suited to blocking a disulfide bond.

Abstract: A method for analyzing droplets based on images: obtaining a working dye channel image and a reference dye channel image corresponding to a droplet system (S1); separately performing a preset image processing operation on the working dye channel image and the reference dye channel image (S2); using the working dye channel image or the reference dye channel image as a target image (S3), and determining a region in which each droplet is located from within the target image (S4); and on the basis of the determined region in which each droplet is located, analyzing the droplet system (S5). The method can effectively improve the accuracy of droplet analysis. Further provided are a computer device and a storage medium.