Abstract: This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.

Abstract: A photodiode (112, 200, 400, 500) includes a semiconductor substrate (210) having a first surface (211) and a second surface (212, 412, 512), and a light sensing junction (220) located adjacent to the first surface (211). The second surface (212, 412, 512) is located opposite the first surface (211), and the second surface (212, 412, 512) includes a concave surface covering a recessed region (415, 515) in the semiconductor substrate (210).

Abstract: Provided is a DNA polymerase mutant for sequencing. The DNA polymerase mutant includes: compared with a Pyrococcus abyssi DNA polymerase exo-mutant, at least three amino acid mutations in the following four sites or functionally equivalent sites: position 409, position 410, position 411, and position 486. The Pyrococcus abyssi DNA polymerase exo-mutant has an amino acid sequence as set forth in SEQ ID NO: 1.

Abstract: Systems and methods for immersion imaging of a flowcell. In one example, an imaging system includes liquid ports in leading and trailing positions relative to a scanned imaging objective. The leading liquid port dispenses an immersion liquid into a space between the distal end of the imaging objective and the flowcell's cover, with the trailing liquid port collecting the immersion liquid. In another example, an imaging system includes a flowcell with a cover that is moveable relative a substrate. The system is configured to move the imaging objective and flowcell cover as a unit relative to the flowcell substrate.

Abstract: Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

Abstract: Provided is a synchronous sequencing method, including: constructing a sequencing library for a nucleic acid sample to be tested; loading the sequencing library onto a sequencing chip; performing a plurality of synchronous sequencing reaction cycles on the sequencing library, wherein an image set generated in each of the plurality of synchronous sequencing reaction cycles constitutes a raw image set of the synchronous sequencing; acquiring a base-calling result of the synchronous sequencing based on the raw image set of the synchronous sequencing; correcting the signal intensity value of each base channel based on a predetermined correction parameter to obtain a corrected base-calling result; and determining a base output result of the synchronous sequencing based on the corrected base-calling result.

Abstract: Embodiments of the disclosure include methods and systems for nucleic acid sequencing that may include an objective coupled to an actuator, wherein the actuator is configured to move the objective over a surface of a substrate. In some embodiments, a droplet may be disposed on the surface of the substrate, and the droplet may be moved along with the objective. The distal end of the objective may include a material that provides a higher friction against the droplet than a material of the surface of the substrate. In some embodiments, the distal end of the objective may be immersed in a fluid as it is moved over the surface of the substrate. The substrate may include vertical walls within a region to retain the fluid.

Abstract: Methods and compositions for preparing a nucleic acid sequencing library are described including (a) transposing an insertion sequence into first fragments of the target nucleic acid, wherein the insertion sequence comprises a hybridization sequence, and wherein the transposing produces nicks in the first fragments; (b) combining in a single mixture (i) the first fragments of the target nucleic acid from (a), (ii) a splint oligonucleotide, and (iii) a population of beads, wherein each bead comprises capture oligonucleotides immobilized thereon, and (c) ligating capture oligonucleotides of individual beads to inserted hybridization sequences of individual first fragments.

Abstract: This application relates to methods and compositions used for paired-end sequencing. The method comprises providing a DNA array comprising a surface immobilized with DNA concatemers. For each of the plurality of DNA concatemers on the array, the method comprises annealing first read primers to primer binding sites on the DNA concatemer, extending at least some of the first read primers to incorporate dNTPs or dNTP analogs, thereby producing first read strands. Each of the dNTPs or dNTP analogs being incorporated is identified to produce first reads, performing controlled MDA by extending at least some of the first read strands with a polymerase having strand-displacement activity to generate a plurality of partially hybridized second strands. Second read primers are then annealed to the single-stranded branches of the plurality of second strands to generate the second reads.

Abstract: Provided are a reagent kit and use thereof in sequencing. The reagent kit includes a first reagent and a second reagent. The first reagent is selected from a disulfide bond reducing agent. The second reagent is selected from a blocking reagent suited to blocking a disulfide bond.

Abstract: A method for analyzing droplets based on images: obtaining a working dye channel image and a reference dye channel image corresponding to a droplet system (S1); separately performing a preset image processing operation on the working dye channel image and the reference dye channel image (S2); using the working dye channel image or the reference dye channel image as a target image (S3), and determining a region in which each droplet is located from within the target image (S4); and on the basis of the determined region in which each droplet is located, analyzing the droplet system (S5). The method can effectively improve the accuracy of droplet analysis. Further provided are a computer device and a storage medium.

Abstract: An apparatus for forming a plurality of microdroplets (26a, 26?, 56) from a droplet (16, 26, 263) includes a substrate (12, 22, 22b, 22c, 1101), a dielectric layer (13, 23, 23b, 23c, 43a, 43b, 43) on the substrate (12, 22, 22b, 22c, 1101) and having a plurality of hydrophilic surface regions (48a, 491, 49) spaced apart from each other by a hydrophobic surface (44, 46), and a plurality of electrodes (14, 14a, 14b, 24, 24a, 24b, 24c, 34a, 34b, 34c) in the dielectric layer (13, 23, 23b, 23c, 43a, 43b, 43).

Abstract: A fluid transportation system and method use a negative pressure driving mode to absorb fluids from a fluid storage assembly and a positive pressure driving mode to load the absorbed fluids into the fluid-using system. At least two working modules are used to transport fluids to the fluid-using system, the process of transporting fluid in each working module being divided into the processes of absorbing fluids into a fluid transfer assembly, and loading fluids into a fluid transfer assembly. The process of absorbing fluids in each working module and the processes of absorbing fluids and/or loading fluids in any other working module overlap at least partially along the time axis. With the present disclosure, the accuracy and speed of fluids transportation are improved, a device utilizing the system and method is also disclosed.

Abstract: A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.

Abstract: An optical imaging system (1) is configured for photographing a sample and includes a lighting module (11) and an imaging module (15). The lighting module (11) is configured for outputting excitation light, the excitation light is configured to excite the sample to generate excited light, the imaging module (15) comprises a time delay integration line scan camera (151), the time delay integration line scan camera (151) is configured to record the excited light. A biochemical substance detection system using the optical imaging system (1) is also provided, improving the detection flux.

Abstract: An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.

Abstract: A flow cell includes a flow cell body. The flow cell body includes a frame and a fluid chamber defined in the flow cell body. The fluid chamber includes a reaction region allowing a fluid flow. A liquid inlet, a liquid outlet, and two exhaust holes connected to the fluid chamber are in the frame. Fluid into the liquid inlet flows through the reaction region in the fluid chamber and flows out through the liquid outlet. The exhaust holes discharge gas generated in the fluid chamber during the fluid flow. A flow cell with integral sealing rings and a biochemical substance reaction device are also disclosed.

Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.

Abstract: A microfluidic apparatus (100) can include a PCB (110), a biological chip (120) overlying the PCB (110), and a microfluidic housing (130) overlying the biological chip (120) and the PCB (110). The microfluidic apparatus (100) also has a first adhesive layer (141) attaching the microfluidic housing (130) to the biological chip (120) and a second adhesive layer (142) attaching the microfluidic housing (130) to the PCB (110). The second adhesive layer (142) is thicker than the first adhesive layer (141). The first adhesive layer (141) comprises a first adhesive material, and the second adhesive layer (142) comprises a second adhesive material.