Patents Assigned to National Institute of Agrobiological Science
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Patent number: 10322185Abstract: The present invention can provide a controlled drug release carrier formed by using a silk fibroin porous material, which has high drug controlled release rate, controllability of the drug controlled release speed, high strength, easy handleability, skin care properties from high biocompatibility, high water retentivity, and capability of efficiently retaining a drug.Type: GrantFiled: April 24, 2013Date of Patent: June 18, 2019Assignees: HITACHI CHEMICAL COMPANY, LTD., ST. MARIANNA UNIVERSITY, SCHOOL OF MEDICINE, NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Kazutoshi Kobayashi, Kunihiro Suto, Naosuke Sumi, Hajime Inoue, Yasushi Tamada
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Patent number: 9828611Abstract: This invention is intended to develop a promoter that can strongly induce marker gene expression throughout an embryo, so as to simply, efficiently, and accurately identify a transgenic insect at an early developmental stage, and to provide a gene expression vector into which such promoter has been incorporated as a transformant discrimination marker. Such exogenous gene expression vector comprises a polynucleotide comprising the nucleotide sequence as shown in SEQ ID NO: 1 as a promoter.Type: GrantFiled: August 6, 2014Date of Patent: November 28, 2017Assignee: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Takuya Tsubota, Keiro Uchino, Hideki Sezutsu, Hiromitsu Tanaka
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Patent number: 9605271Abstract: The present invention relates to plants that have been transformed so as to have both disease resistance and acceptable agronomic traits. More specifically, the present invention relates to transgenic plants that have acquired disease resistance through expression in the plants of a polynucleotide encoding the transcription factor WRKY45 in an infection-responsive manner, and methods for generating the transgenic plants.Type: GrantFiled: January 23, 2013Date of Patent: March 28, 2017Assignee: National Institute of Agrobiological SciencesInventors: Hiroshi Takatsuji, Shingo Goto
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Patent number: 9593343Abstract: This invention is intended to allow accumulation of large quantities of soluble sugars in tissue other than plant seeds. A plant is modified so as to suppress a gene encoding a subunit exhibiting the highest sequence similarity with the subunit encoded by the AGPL1 gene of rice among subunits constituting.Type: GrantFiled: March 22, 2012Date of Patent: March 14, 2017Assignees: TOYOTA JIDOSHA KABUSHIKI KAISHA, THE UNIVERSITY OF TOKYO, INCORPORATED ADMINISTRATIVE AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION, NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Madoka Yonekura, Naohiro Aoki, Ryu Ohsugi, Masaki Okamura, Tatsuro Hirose, Chikara Ohto, Akio Miyao
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Publication number: 20160281103Abstract: The Vrs1 gene was found to be highly expressed in the pistils of sterile lateral spikelets of two-row barley, showing that the VRS1 protein suppresses the fertility of florets in lateral spikelets. In addition, it was also found that the introduction of siRNA specific to the Vrs1 gene into two-row barley successfully restored the fertility of lateral spikelets and thus can increase the number of grains. The vrs1 gene derived from wheat was isolated for the first time, and the expression site of the gene was found to be specific to upper florets to be sterile in spikelets. Furthermore, it was confirmed that the introduction of siRNA specific to the wheat Vrs1 gene into wheat successfully increased the number of florets and the number of grains per spikelet, enabling provision of a method and an agent for increasing the number of grains per spikelet of wheat.Type: ApplicationFiled: July 8, 2013Publication date: September 29, 2016Applicant: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Takao Komatsuda, Takashi Matsumoto, Shun Sakuma, Taiichi Ogawa
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Patent number: 9440005Abstract: A substrate for feeding cells and/or tissues which is composed of a porous body having a thin film surface and a porous surface and which can be used in the regenerative medical techniques. A cell/tissue feeder is produced by cultivating tissues on the porous surface of the porous body. Cells are seeded on the porous surface of the body, followed by the cultivation of the cells to thus form and/or regenerate tissues. An aqueous solution of silk proteins, to which a water-soluble organic solvent is added, is introduced into a mold whose bottom or top surface is surface-roughened, followed by freezing the aqueous solution and then thawing the frozen aqueous solution to form a porous body having a thin film surface and a porous surface.Type: GrantFiled: August 23, 2010Date of Patent: September 13, 2016Assignees: National Institute of Agrobiological Sciences, Kyoto UniversityInventors: Yasushi Tamada, Katsura Kojima, Naohide Tomita, Eiichi Hirakata
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Patent number: 9434958Abstract: The present inventors isolated many promoters having various expression characteristics from monocots, connected the OsWRKY45 gene downstream of these promoters, and then re-introduced them into a monocot (rice plant), and thereby strived to produce a rice line having both complex disease resistance and excellent agronomic traits. As a result, the present inventors succeeded in producing transgenic plants having both disease resistance and good agronomic traits by expressing OsWRKY45 using upstream sequences of the EF1? or OsUbi7 gene as promoters.Type: GrantFiled: March 1, 2012Date of Patent: September 6, 2016Assignee: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Hiroshi Takatsuji, Shingo Goto
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Patent number: 9359416Abstract: Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.Type: GrantFiled: September 16, 2014Date of Patent: June 7, 2016Assignees: NITTO BOSEKI CO., LTD., NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Iwao Kiyokawa, Yuji Arimatsu, Toshihide Miura, Ryo Kojima, Hideki Sezutsu, Keiro Uchino, Isao Kobayashi, Toshiki Tamura
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Publication number: 20160138032Abstract: It has been found that introducing into a Poaceae plant an Hd3a gene, which is a flower-bud-formation inducing gene, positioned downstream of a promoter whose expression is induced by a plant activator treatment makes it possible to control the flowering time of the Poaceae plant in accordance with a plant activator treatment timing. It has been found that further introducing a Ghd7 gene, which functions to suppress flower bud formation, into the plant makes it possible to suppress the expression of an endogenous Hd3a gene and increase the efficiency of controlling the flowering time.Type: ApplicationFiled: March 4, 2014Publication date: May 19, 2016Applicants: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES, INCORPORATED ADMINISTRATIVE AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATIONInventors: Takeshi IZAWA, Ryo OKADA, Naokuni ENDO, Yasue NEMOTO, Tadashi TAKAMIZO, Shoko TSUZUKI, Satoru NISHIMURA
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Publication number: 20160135946Abstract: The present invention addresses the problem of providing an artificial skin preparation which requires no suturing or the like when treating a wound area with a dried collagen vitrigel membrane material as an artificial skin, and also is less likely to cause contamination or the like with an exudate, and is free from the risk of secondary damage by replacement thereof.Type: ApplicationFiled: June 24, 2014Publication date: May 19, 2016Applicants: Saga University, National Institute of Agrobiological Sciences, Yutoku Pharmaceutical Industries Co., Ltd.Inventors: Shigehisa AOKI, Toshiaki TAKEZAWA, Ayumi MIYAZAKI, Hiroshi HIRAYAMA
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Patent number: 9328135Abstract: A method for producing a porous silk fibroin material, containing steps of: freezing a silk fibroin solution containing a silk fibroin aqueous solution having an aliphatic carboxylic acid added thereto; and then melting the frozen solution, thereby providing a porous material. A method for producing a porous silk fibroin material having excellent mechanical characteristics may be provided.Type: GrantFiled: January 7, 2013Date of Patent: May 3, 2016Assignees: Hitachi Chemical Company, Ltd., National Institute of Agrobiological SciencesInventors: Kazutoshi Kobayashi, Naosuke Sumi, Tsuyoshi Abe, Yasushi Tamada
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Patent number: 9260491Abstract: It is an object of the present invention to provide a protein that can successfully treats almost all patients with cedar pollinosis and can be ingested as a food. That is, the present invention provides a protein comprising an amino acid sequence represented by SEQ ID NO:1 or comprising an amino acid sequence having a mutation(s) in said amino acid sequence, and having an immunogenicity of a cedar pollen; a polynucleotide encoding said protein; a vector comprising said polynucleotide; a transformant comprising said polynucleotide or said vector; and intended uses of said protein, said polynucleotide, and said transformant.Type: GrantFiled: October 28, 2010Date of Patent: February 16, 2016Assignees: NIPPON PAPER INDUSTRIES CO., LTD., NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Fumio Takaiwa, Hidenori Takagi, Yuhya Wakasa, Yuji Fujii, Tomotaka Shinya, Saori Kasahara
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Patent number: 9127290Abstract: A three-step screening of selection for resistance against pathogenic bacteria infection, selection for resistance against pathogenic fungi infection, and selection for sensitivity to salicylic acid was carried out on Arabidopsis thaliana lines highly expressing rice full-length cDNA (rice-FOX Arabidopsis lines) which were prepared by using a FOX hunting system. As a result, one Arabidopsis thaliana line (line K15424) selected in all of the three types of screening was successfully selected. Line K15424 carries a rice full-length cDNA. Rice overexpressing AK070024 was produced, and resistance against rice bacterial leaf blight was assayed using the T1 generation. As a result, it was confirmed that AK070024-overexpressing rice is resistant against bacterial leaf blight and blast.Type: GrantFiled: March 4, 2009Date of Patent: September 8, 2015Assignees: National Institute of Agrobiological Sciences, RikenInventors: Masaki Mori, Nagao Hayashi, Shoji Sugano, Hiroshi Takatsuji, Hirohiko Hirochika, Kenji Oda, Minami Matsui
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Patent number: 9097705Abstract: The present invention has successfully established a mixed culture system capable of actively proliferating a Kupffer cell in a primary culture of a cell population derived from a liver. Additionally, the present invention has successfully established a novel production method for efficiently producing a large amount of highly purified Kupffer cells using this mixed culture system.Type: GrantFiled: October 8, 2010Date of Patent: August 4, 2015Assignee: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Hiroshi Kitani, Takato Takenouchi, Noriko Yamanaka, Miyako Yoshioka
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Publication number: 20150101081Abstract: The present invention provides a method for transforming organelles having an own genomic DNA, the method enabling efficient production of highly homoplasmic plant cells, in which most of the organelles are transformed, and so forth. The method comprises the steps of: expressing in plant cells a fusion protein containing a function inhibiting factor of the organelles and a transit signal peptide to the organelles; introducing into the genomic DNA of the organelles of the plant cells an expression cassette comprising DNAs encoding a restoring factor of the organelles and a factor desired to be expressed in the organelles; and allowing the function inhibiting factor to destroy the organelles, in which the expression cassette is not introduced, in the plant cells.Type: ApplicationFiled: November 22, 2012Publication date: April 9, 2015Applicant: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Yutaka Tabei, Ayako Okuzaki
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Publication number: 20150094262Abstract: The present invention can provide a controlled drug release carrier formed by using a silk fibroin porous material, which has high drug controlled release rate, controllability of the drug controlled release speed, high strength, easy handleability, skin care properties from high biocompatibility, high water retentivity, and capability of efficiently retaining a drug.Type: ApplicationFiled: April 24, 2013Publication date: April 2, 2015Applicants: Hitachi Chemical Company, Ltd., St. Marianna University, School of Medicine, National Institute of Agrobiological SciencesInventors: Kazutoshi Kobayashi, Kunihiro Suto, Naosuke Sumi, Hajime Inoue, Yasushi Tamada
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Patent number: 8952215Abstract: The present inventors produced transgenic silkworms which comprise a promoter of a DNA encoding a protein specifically expressed in the silk gland and a DNA encoding a recombinant antibody whose expression is regulated directly or indirectly by the promoter, and which secrete the recombinant antibody into the silk gland. The recombinant antibodies produced from the silk gland of the transgenic silkworms were confirmed to be active.Type: GrantFiled: October 18, 2006Date of Patent: February 10, 2015Assignees: Nitto Boseki Co., Ltd., National Institute of Agrobiological SciencesInventors: Toshiki Tamura, Isao Kobayashi, Toshio Kanda, Keiro Uchino, Katsuhiro Katayama, Tatsuya Ohashi, Iwao Kiyokawa, Hisae Arai, Noriyuki Funahashi
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Patent number: 8865966Abstract: Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.Type: GrantFiled: May 30, 2011Date of Patent: October 21, 2014Assignees: Nitto Boseki Co., Ltd., National Institute of Agrobiological SciencesInventors: Iwao Kiyokawa, Yuji Arimatsu, Toshihide Miura, Ryo Kojima, Hideki Sezutsu, Keiro Uchino, Isao Kobayashi, Toshiki Tamura
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Patent number: 8859852Abstract: The present invention is directed to identify genes involved in the growth of a plant and provide a transformed plant the growth of which is promoted utilizing the genes.Type: GrantFiled: December 5, 2008Date of Patent: October 14, 2014Assignees: Riken, National Institute of Agrobiological SciencesInventors: Shinya Takahashi, Takanari Ichikawa, Minami Matsui, Tomoko Kuriyama, Yukako Hasegawa, Hirohiko Hirochika, Masaki Mori
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Publication number: 20140283209Abstract: The present inventors isolated many promoters having various expression characteristics from monocots, connected the OsWRKY45 gene downstream of these promoters, and then re-introduced them into a monocot (rice plant), and thereby strived to produce a rice line having both complex disease resistance and excellent agronomic traits. As a result, the present inventors succeeded in producing transgenic plants having both disease resistance and good agronomic traits by expressing OsWRKY45 using upstream sequences of the EF1? or OsUbi7 gene as promoters.Type: ApplicationFiled: March 1, 2012Publication date: September 18, 2014Applicant: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCESInventors: Hiroshi Takatsuji, Shingo Goto