Abstract: The present invention provides a method for identifying animal species, said method comprises a step of amplifying a DNA fragment by PCR using a DNA in a sample as a template and animal-specific DNA sequences as a primer pair, wherein the animal-specific DNA sequences are derived from a ATP synthase subunit 8 gene or a region proximal thereto of a mitochondrial genome; and a step of detecting the amplified DNA fragment.
Type:
Application
Filed:
February 20, 2014
Publication date:
August 21, 2014
Applicants:
Incorporated Administrative Agency Food And Agricultural Materials Inspection, NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
Abstract: The present invention relates to plants that have been transformed so as to have both disease resistance and acceptable agronomic traits. More specifically, the present invention relates to transgenic plants that have acquired disease resistance through expression in the plants of a polynucleotide encoding the transcription factor WRKY45 in an infection-responsive manner, and methods for generating the transgenic plants.
Type:
Application
Filed:
January 23, 2013
Publication date:
July 24, 2014
Applicant:
National Institute of Agrobiological Sciences
Abstract: Extraction and purification of recombinant proteins rendered difficult to extract from transgenic plants by using an extraction solution containing reducing agents and surfactants or an extraction solution containing reducing agents and organic solvents.
Type:
Grant
Filed:
September 20, 2011
Date of Patent:
June 24, 2014
Assignees:
National Institute of Agrobiological Sciences, Preventec, Inc.
Abstract: A compound useful as a plant activator for activating an endogenous defense system of a plant to control disease damage is provided. A compound represented by the formula: (R3)NH—(CH2)4—N(R1)—(CH2)3—NH(R2) (one of R1 and R2 represents a linear C6-18 alkanoyl group or alkenoyl group, the other represents hydrogen atom or a protective group of amino group; and R3 represents hydrogen atom or a protective group of amino group).
Type:
Grant
Filed:
February 23, 2012
Date of Patent:
June 3, 2014
Assignees:
The University of Tokyo, National Institute of Agrobiological Sciences, Riken
Abstract: This invention is intended to allow accumulation of large quantities of soluble sugars in tissue other than plant seeds. A plant is modified so as to suppress a gene encoding a subunit exhibiting the highest sequence similarity with the subunit encoded by the AGPL1 gene of rice among subunits constituting.
Type:
Application
Filed:
March 22, 2012
Publication date:
March 27, 2014
Applicants:
TOYOTA JIDOSHA KABUSHIKI KAISHA, NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES, THE UNIVERSITY OF TOKYO
Abstract: An insect-resistant protein exhibiting a sufficient resistance to insects; an insect-resistance gene encoding the insect-resistant protein; a recombinant vector containing the insect-resistance gene; a host cell and a plant cell having the recombinant vector transfected thereinto; a transformant transformed by the insect-resistance gene and a method for producing the same; a protein recovered thereby; and an insect-resistant agent comprising them as active ingredients. In accordance with the present invention, the insect-resistant protein is derived from a plant.
Type:
Grant
Filed:
March 3, 2008
Date of Patent:
January 7, 2014
Assignees:
National Institute of Agrobiological Sciences
Abstract: The present inventors identified a novel rice glutelin gene, GluD-1, which is expressed specifically in seeds. The promoter of the GluD-1 gene was confirmed to induce seed-specific gene expression, and to induce expression of downstream genes specifically in the endosperm during the early stage of seed maturation process. More specifically, the GluD-1 promoter can induce strong expression of an exogenous gene in sites including the endosperm.
Type:
Grant
Filed:
April 10, 2009
Date of Patent:
October 8, 2013
Assignee:
National Institute of Agrobiological Sciences
Abstract: A compound useful as a plant activator for activating an endogenous defense system of a plant to control disease damage is provided. A compound represented by the formula: (R3)NH—(CH2)4—N(R1)—(CH2)3—NH(R2) (one of R1 and R2 represents a linear C6-18 alkanoyl group or alkenoyl group, the other represents hydrogen atom or a protective group of amino group; and R3 represents hydrogen atom or a protective group of amino group).
Type:
Application
Filed:
February 23, 2012
Publication date:
July 4, 2013
Applicants:
THE UNIVERSITY OF TOKYO, RIKEN, NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
Abstract: This invention provides a method for cancer diagnosis comprising measuring an endogenous antisense RNA whose relative expression to a sense RNA changes cancer-specifically in RNA-containing samples collected from a mammal. Also provided are endogenous antisense RNAs useful as cancer markers, and cancer diagnostic reagents containing the same.
Type:
Grant
Filed:
May 30, 2008
Date of Patent:
May 7, 2013
Assignees:
Riken, National Institute of Agrobiological Sciences, University of Tsukuba
Abstract: Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.
Type:
Grant
Filed:
December 28, 2007
Date of Patent:
April 23, 2013
Assignees:
Nitto Boseki Co., Ltd., National Institute of Agrobiological Sciences
Abstract: It has been found that the use of a plant cell in which a function of a protein involved in repair by nonhomologous end joining is artificially suppressed dramatically increases the efficiency of introductions of non-silent mutations in a repairing process by nonhomologous end joining which occurs after induction of a DNA double-strand break with a zinc finger nuclease.
Type:
Application
Filed:
October 25, 2010
Publication date:
March 21, 2013
Applicant:
NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
Abstract: The present inventors analyzed genes involved in plant disease resistance. As a result, they discovered the transcription factor gene OsWRKY45, which is induced in leaf blades of rice plants by activating the systemic acquired resistance (SAR) that plants inherently have, i.e., by treating rice plants with benzothiadiazole (hereafter may be abbreviated as BTH), which is an agent thought to induce disease resistance in plants. It was further discovered that rice blast disease resistance and rice bacterial leaf blight resistance were markedly improved by reintroducing the gene into rice plants and constitutively expressing it.
Type:
Grant
Filed:
May 26, 2006
Date of Patent:
February 12, 2013
Assignee:
National Institute of Agrobiological Sciences
Abstract: Providing a new method of treating inflammatory gastrointestinal disorders and a food product for the prevention or improvement of inflammatory gastrointestinal disorders. Prevention or treatment of inflammatory gastrointestinal disorders is possible by oral administration of recombinant IL-10 expressed in rice plant seeds.
Type:
Application
Filed:
November 30, 2011
Publication date:
December 27, 2012
Applicants:
National Institute of Agrobiological Sciences, National Institute of Advanced Industrial Science and Technology, Preven Tec, Inc.
Abstract: To provide a gene that controls the deep rooting of a plant, a transgenic plant introduced with the gene, a method for controlling the deep rooting of a plant using the gene, and such, high-resolution linkage analysis was performed for a genetic locus (Dro1 locus) capable of controlling the deep rooting of a plant, which was detected between a shallow-rooted rice cultivar IR64 and a deep-rooted rice cultivar Kinandang Patong in a large-scale segregating population. As a result, it was revealed that the gene region of Dro1 is located in a region of 6.0 kbp sandwiched between Dro1-INDEL09, which is an InDel marker, and Dro1-CAPS05, which is a CAPS marker. Furthermore, it was confirmed that a transgenic plant transformed with the Kinandang Patong-type Dro1 gene shows a significantly high ratio of deep rooting. It was also confirmed that a plant having the Kinandang Patong-type Dro1 gene is resistant to drought.
Type:
Application
Filed:
December 24, 2010
Publication date:
December 13, 2012
Applicant:
National Institute of Agrobiological Sciences
Abstract: The present invention provides a method for identifying animal species, said method comprises a step of amplifying a DNA fragment by PCR using a DNA in a sample as a template and animal-specific DNA sequences as a primer pair, wherein the animal-specific DNA sequences are derived from a ATP synthase subunit 8 gene or a region proximal thereto of a mitochondrial genome; and a step of detecting the amplified DNA fragment.
Type:
Application
Filed:
March 12, 2012
Publication date:
October 25, 2012
Applicants:
INCORPORATED ADMINISTRATIVE AGENCY FERTILIZER AND FEED INSPECTION SERVICES, NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
Abstract: A novel gene controlling chasmogamy/cleistogamy of barley was successfully identified, and the mechanism of development of chasmogamy/cleistogamy of barley was successfully elucidated by positional cloning approach. In addition, it has been found that the use of the identified gene makes it possible to specifically and efficiently determine chasmogamy/cleistogamy of a plant and impart a cleistogamous trait to a plant.
Type:
Application
Filed:
October 15, 2010
Publication date:
October 18, 2012
Applicant:
NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
Abstract: It is an object of the present invention to provide a protein that can successfully treats almost all patients with cedar pollinosis and can be ingested as a food. That is, the present invention provides a protein comprising an amino acid sequence represented by SEQ ID NO:1 or comprising an amino acid sequence having a mutation(s) in said amino acid sequence, and having an immunogenicity of a cedar pollen; a polynucleotide encoding said protein; a vector comprising said polynucleotide; a transformant comprising said polynucleotide or said vector; and intended uses of said protein, said polynucleotide, and said transformant.
Type:
Application
Filed:
October 28, 2010
Publication date:
September 27, 2012
Applicants:
NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES, NIPPON PAPER INDUSTRIES CO., LTD.
Abstract: The present invention has successfully established a mixed culture system capable of actively proliferating a Kupffer cell in a primary culture of a cell population derived from a liver. Additionally, the present invention has successfully established a novel production method for efficiently producing a large amount of highly purified Kupffer cells using this mixed culture system.
Type:
Application
Filed:
October 8, 2010
Publication date:
August 9, 2012
Applicant:
NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
Abstract: A substrate for feeding cells and/or tissues which is composed of a porous body having a thin film surface and a porous surface and which can be used in the regenerative medical techniques. A cell/tissue feeder is produced by cultivating tissues on the porous surface of the porous body. Cells are seeded on the porous surface of the body, followed by the cultivation of the cells to thus form and/or regenerate tissues. An aqueous solution of silk proteins, to which a water-soluble organic solvent is added, is introduced into a mold whose bottom or top surface is surface-roughened, followed by freezing the aqueous solution and then thawing the frozen aqueous solution to form a porous body having a thin film surface and a porous surface.
Type:
Application
Filed:
August 23, 2010
Publication date:
June 14, 2012
Applicants:
KYOTO UNIVERSITY, NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
Abstract: Extraction and purification of recombinant proteins rendered difficult to extract from transgenic plants by using an extraction solution containing reducing agents and surfactants or an extraction solution containing reducing agents and organic solvents.
Type:
Application
Filed:
September 20, 2011
Publication date:
June 7, 2012
Applicants:
PrevenTec, Inc., National Institute of Agrobiological Science