Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Application
Filed:
December 26, 2006
Publication date:
March 20, 2008
Applicant:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
Abstract: The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.
Type:
Grant
Filed:
January 10, 2005
Date of Patent:
March 4, 2008
Assignee:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David A. Zopf, Zhi-Guang Wang, Henrik Clausen
Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Application
Filed:
January 11, 2007
Publication date:
February 28, 2008
Applicant:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
Abstract: This invention provides nucleic acid and amino acid sequences of fucosyltransferases from Helicobactor pylori. The invention also provides methods to use the fucosyltransferases to synthesize oligosaccharides, glycoproteins, and glycolipids.
Type:
Grant
Filed:
January 22, 2004
Date of Patent:
February 5, 2008
Assignees:
Neose Technologies, Inc., Governors of the University of Alberta
Inventors:
Joanne Simala-Grant, Diane Taylor, Karl F. Johnson, Daniel James Bezila
Abstract: Novel synthetic glycosphingolipids and pharmaceutical compositions containing such synthetic glycosphingolipids are described. Methods of making the novel synthetic glycosphingolipid compounds and compositions as well as their use in the field of neuroprotection and cancer treatment is also described.
Type:
Application
Filed:
March 4, 2004
Publication date:
November 29, 2007
Applicant:
Neose Technologies, Inc.
Inventors:
Shawn Defrees, Karl Johnson, Zhi-Guang Wang
Abstract: The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods of refolding more than one glycosyltransferase in a single vessel, methods to use such refolded glycosyltransferases, and reaction mixtures comprising the refolded glycosyltransferases.
Type:
Application
Filed:
February 4, 2005
Publication date:
November 22, 2007
Applicant:
Neose Technologies, Inc.
Inventors:
Sami Saribas, David Hakes, Scott Willett, Karl Johnson, Daniel Bezila, Shawn Defrees
Abstract: The invention includes a multitude of methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Grant
Filed:
April 9, 2003
Date of Patent:
November 20, 2007
Assignee:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group a peptide.
Type:
Grant
Filed:
April 12, 2006
Date of Patent:
October 2, 2007
Assignee:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David A. Zopf, Caryn Bowe
Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Grant
Filed:
April 9, 2003
Date of Patent:
September 4, 2007
Assignee:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David A. Zopf, Robert J. Bayer, Caryn Bowe, David James Hakes, Xi Chen
Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Grant
Filed:
April 9, 2003
Date of Patent:
September 4, 2007
Assignee:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
Abstract: This invention provides methods for practical enzymatic conversion of GDP-mannose to GDP-fucose. These methods are useful for efficient synthesis of reactants used in the synthesis of fucosylated oligosaccharides.
Abstract: The present invention relates to protease resistant mutants of human growth hormone, which contain newly introduced proteolysis resistant mutations and N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.
Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Grant
Filed:
April 9, 2003
Date of Patent:
June 5, 2007
Assignee:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
Abstract: This invention provides methods for practical in vitro sialylation of glycoproteins, including recombinantly produced glycoproteins. The methods are useful for large-scale modification of sialylation patterns.
Type:
Grant
Filed:
February 21, 2002
Date of Patent:
May 22, 2007
Assignee:
Neose Technologies, Inc.
Inventors:
James C. Paulson, Robert J. Bayer, Eric Sjoberg
Abstract: The present invention provides conjugates between peptides and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.
Type:
Application
Filed:
October 13, 2006
Publication date:
May 10, 2007
Applicant:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David Zopf, Susann Taudte, Walter Willett, Robert Bayer, Matthew Kalo
Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Grant
Filed:
April 9, 2003
Date of Patent:
May 8, 2007
Assignee:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David A. Zopf, Robert J. Bayer, Caryn Bowe, David James Hakes, Xi Chen
Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Grant
Filed:
April 9, 2003
Date of Patent:
February 20, 2007
Assignee:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David James Hakes, Xi Chen
Abstract: The present invention provides branched water-soluble polymers that allow two or more water-soluble polymers to be conjugated to another species. The branched polymers provide access to therapeutic agents that are conjugated at a single site to two or more water-soluble polymers. The branched polymers are based upon branch points that are simple branched alkyl structures, reactive side-chain amino acids and small peptides of reactive side-chain amino acids, and saccharides. Also provided is a method for preparing mono-disperse poly(ethylene glycol) of a well-defined and determinable molecular weight, and a method for the rational end-functionalization of poly(ethylene glycol). Conjugates of the branched water-soluble polymers with diverse species, e.g., peptides, lipids, glycolipids and small molecules are also provided.
Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Grant
Filed:
April 9, 2003
Date of Patent:
February 6, 2007
Assignee:
Neose Technologies, Inc.
Inventors:
Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
Type:
Application
Filed:
April 9, 2004
Publication date:
February 1, 2007
Applicant:
NEOSE TECHNOLOGIES, INC.
Inventors:
Shawn DeFrees, David Zopf, Robert Bayer, David Hakes, Caryn Bowe, Xi Chen