Patents Assigned to Neose Technologies, Inc.
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Patent number: 6399336Abstract: This invention provides methods for practical in vitro sialylation of glycoproteins, including recombinantly produced glycoproteins. The methods are useful for large-scale modification of sialylation patterns.Type: GrantFiled: January 15, 1998Date of Patent: June 4, 2002Assignee: Neose Technologies, Inc.Inventors: James C. Paulson, Robert J. Bayer, Eric Sjoberg
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Patent number: 6379933Abstract: The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.Type: GrantFiled: June 24, 1999Date of Patent: April 30, 2002Assignee: Neose Technologies, Inc.Inventors: Karl F. Johnson, Stephen Roth, Stephanie L. Buczala
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Patent number: 6331418Abstract: A method for preparing saccharide compositions which is reiterative and includes the following three steps. (i) A glycosyltransferase capable of transferring a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate. (ii) The isolated glycosyltransferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit. (iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.Type: GrantFiled: June 7, 1995Date of Patent: December 18, 2001Assignee: Neose Technologies, Inc.Inventor: Stephen Roth
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Patent number: 6323008Abstract: The present invention provides methods for producing sialyloligosaccharides in situ in dairy sources and cheese processing waste streams, prior to, during, or after processing of the dairy source during the cheese manufacturing process. The methods of the present invention use the catalytic activity of &agr;(2-3) trans-sialidases to exploit the high concentrations of lactose and &agr;(2-3) sialosides which naturally occur in dairy sources and cheese processing waste streams to drive the enzymatic synthesis of &agr;(2-3) sialyllactose. &agr;(2-3) sialyloligosaccharides produced according to these methods are additionally encompassed by the present invention. The invention also provides for recovery of the sialyloligosaccharides produced by these methods. The invention further provides a method for producing &agr;(2-3) sialyllactose. The invention additionally provides a method of enriching for &agr;(2-3) sialyllactose in milk using transgenic mammals that express an &agr;(2-3) trans-sialidase transgene.Type: GrantFiled: August 14, 1997Date of Patent: November 27, 2001Assignee: Neose Technologies, Inc.Inventors: Marc Pelletier, William A. Barker, David J. Hakes, David A. Zopf
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Patent number: 6288222Abstract: This invention relates to a method of filtering of a dairy stream.Type: GrantFiled: February 16, 2000Date of Patent: September 11, 2001Assignee: Neose Technologies, Inc.Inventors: Stephen A. Roth, Jonathan K. Weil, Michael Spade
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Patent number: 6284493Abstract: A method of synthesizing saccharide compositions is described. In this method, an acceptor moiety is contacted with at least one donor saccharide in the presence of at least one cell surface-bound glycosyltransferase specific for catalyzing the coupling of the acceptor moiety with the donor saccharide. The acceptor moiety used is a carbohydrate, a protein, a lipid, or a glycolipid.Type: GrantFiled: March 9, 1999Date of Patent: September 4, 2001Assignee: Neose Technologies, Inc.Inventor: Stephen Roth
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Patent number: 6127153Abstract: The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.Type: GrantFiled: June 7, 1995Date of Patent: October 3, 2000Assignee: Neose Technologies, Inc.Inventors: Karl F. Johnson, Stephen Roth, Stephanie L. Buczala
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Patent number: 6117651Abstract: The present invention provides isolated recombinant nucleic acid constructs comprising a dual bacterial promoter operably linked to a heterologous nucleic acid which encodes a desired polypeptide. The constructs are useful for the expression of the desired polypeptides in bacterial cells at high levels.Type: GrantFiled: November 7, 1997Date of Patent: September 12, 2000Assignee: Neose Technologies, Inc.Inventors: Jody Schultz, Gary Hermanson
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Patent number: 6096725Abstract: The invention relates to methods for attenuating xenograft rejection in humans and old world monkeys, using oligosaccharides containing a Gal.alpha.1-3Gal motif, to neutralize or remove anti-.alpha.Gal antibodies. The invention additionally relates to methods for site directed activation of the complement cascade or host leukocytes using oligosaccharides containing a Gal.alpha.1-3Gal motif to target anti-.alpha.Gal antibodies. The invention further relates to pharmaceutical compositions that may be used in the practice of the invention. Such compositions contain, as the active ingredient, an oligosaccharide containing a Gal.alpha.1-3Gal motif effective in binding anti-.alpha.Gal antibodies in vivo or ex vivo.Type: GrantFiled: July 2, 1997Date of Patent: August 1, 2000Assignee: Neose Technologies, Inc.Inventors: Paul M. Simon, Edward J. McGuire
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Patent number: 6033663Abstract: DNA sequences encoding GDP-fucose pyrophosphorylase (GTP+fucose-1-P.fwdarw.GDP-fucose+PP.sub.i), are provided. The enzymes can be used in the synthesis of desired carbohydrate structures.Type: GrantFiled: April 9, 1997Date of Patent: March 7, 2000Assignee: Neose Technologies, Inc.Inventors: Catherine M. Ketcham, Alan D. Elbein, Richard R. Drake, Irena Pastuszak
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Patent number: 6030815Abstract: The present invention provides improved methods for the preparation of sialyl galactosides. The methods use sialyl transferase cycle in which the reaction conditions are optimized to provide increased yields.Type: GrantFiled: April 10, 1996Date of Patent: February 29, 2000Assignee: Neose Technologies, Inc.Inventors: Shawn DeFrees, Robert J. Bayer, Murray Ratcliffe
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Patent number: 6001819Abstract: The present invention is directed to compositions for the treatment of respiratory infections caused by a bacteria selected from the group consisting of S. pneumoniae, H. influenzae, H. parainfluenza, Burkholderia (Pseudomonas) cepacia, and a mixture thereof, comprising a binding inhibiting effective amount of the compound of Formula I, ##STR1## where R.sub.1 is H, (.beta.-1)GalNAc-, SO.sub.3 B (where B is H or a cation) or a sialic acid of Formula II; ##STR2## where R.sub.6, R.sub.7, R.sub.8, and R.sub.10 are each independently H, C.sub.1-6 acyl, lactyl, C.sub.1-6 alkyl, sulfate, phosphate, anhydro, a sialic acid of Formula II, (.alpha.-1)Fuc, (.beta.-1)Glc or (.beta.-1)Gal;R.sub.9 is NH--C.sub.1-6 acyl, glycolylamido, amino or hydroxyl; andA is H or a cation;R.sub.2 is H or (.alpha.-1)Fuc-;R.sub.3 and R.sub.4 are each independently OH or NHAc;R.sub.5 is H, SO.sub.Type: GrantFiled: April 3, 1998Date of Patent: December 14, 1999Assignee: Neose Technologies, Inc.Inventors: Paul M. Simon, David Zopf, Roger A. Barthelson, Karl F. Johnson
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Patent number: 5998177Abstract: The present invention relates to a process of preparing commercial quantities of glucose from sucrose, a process of preparing commercial quantities of glucose and a branched fructan from sucrose, a reactor for practicing same.Type: GrantFiled: November 19, 1998Date of Patent: December 7, 1999Assignee: Neose Technologies, Inc.Inventors: Steven J. Catani, Stephen A. Roth, Edward J. McGuire, Juan L. Navia
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Patent number: 5952205Abstract: The present invention relates to a process of preparing commercial quantities of glucose and/or fructose from sucrose, a process of preparing commercial quantities of glucose and a polyfructan from sucrose, a reactor for practicing same, a process of preparing commercial quantities of fructose and a polyglucan from sucrose and a reactor for practicing same.Type: GrantFiled: February 6, 1998Date of Patent: September 14, 1999Assignee: Neose Technologies, Inc.Inventors: Steven J. Catani, Stephen A. Roth, Edward J. McGuire, Juan L. Navia
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Patent number: 5883079Abstract: A method for treating and/or inhibiting gastric and duodenal ulcers, comprising administering a pharmaceutical composition comprising an oligosaccharide of Formula I(NeuAc-.alpha.(2-3)-pGal-.beta.(1)-(--X--).sub.m --(--Y--).sub.n -).sub.p -ZwhereinX=a chemical bond or a group capable of linking the p galactose to either the linking group Y or the multivalent support Z;wherein the C.sub.1 glycosidic oxygen of galactose may be replaced by N, S or C;Y=a linking group;Z=a multivalent support;m=0 or 1;n=0 or 1; andp=an integer of 2-1,000 is described. Also described is a method for treating and/or inhibiting gastric and duodenal ulcers, comprising administering a pharmaceutical composition comprising an oligosaccharide of Formula IINeuAc-.alpha.(2-3)-pGal-.beta.(1)-AwhereinA=a group capable of bonding to the p galactose;wherein the C.sub.1 glycosidic oxygen of galactose may be replaced by N, S or C.Type: GrantFiled: May 12, 1998Date of Patent: March 16, 1999Assignee: Neose Technologies, Inc.Inventors: David A Zopf, Paul M. Simon, Stephen Roth, Edward J. McGuire, Dennis H. Langer
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Patent number: 5879912Abstract: A method of synthesizing saccharide compositions is described. In this method, an acceptor moiety is contacted with at least one donor saccharide in the presence of at least one cell surface-bound glycosyltransferase specific for catalyzing the coupling of the acceptor moiety with the donor saccharide. The acceptor moiety used is a carbohydrate, a protein, a glycolipid, a lipid, or a glycolipid.Type: GrantFiled: June 17, 1996Date of Patent: March 9, 1999Assignee: Neose Technologies, Inc.Inventor: Stephen Roth
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Patent number: 5843786Abstract: The present invention is directed to a method of detecting reducing carbohydrates in a complex biological sample. The sample is filtered, subjected to ion exchange chromatography, derivatized, extracted and analyzed by HPLC.Type: GrantFiled: November 28, 1995Date of Patent: December 1, 1998Assignee: Neose Technologies, Inc.Inventors: Daotian Fu, Ming He, David Zopf
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Patent number: 5753630Abstract: A method for treating and/or inhibiting gastric and duodenal ulcers, comprising administering a pharmaceutical composition comprising an oligosaccharide of Formula I(NeuAc-.alpha.(2-3)-pGal-.beta.(1)--(--X--).sub.m --(--Y--).sub.n --).sub.p --ZwhereinX=a chemical bond or a group capable of linking the p galactose to either the linking group Y or the multivalent support Z;wherein the C.sub.1 glycosidic oxygen of galactose may be replaced by N, S or C;Y=a linking group;Z=a multivalent support;m=0 or 1;n=0 or 1; andp=an integer of 2-1,000 is described. Also described is a method for treating and/or inhibiting gastric and duodenal ulcers, comprising administering a pharmaceutical composition comprising an oligosaccharide of Formula IINeuAc-.alpha.(214 3)-pGal-.beta.(1)-AwhereinA=a group capable of bonding to the p galactose;wherein the C.sub.1 glycosidic oxygen of galactose may be replaced by N, S or C.Type: GrantFiled: February 8, 1996Date of Patent: May 19, 1998Assignee: Neose Technologies, Inc.Inventors: David A. Zopf, Paul M. Simon, Stephen Roth, Edward J. McGuire, Dennis H. Langer
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Patent number: 5736533Abstract: The present invention is directed to a method of treating respiratory infections caused by a bacteria selected from the group consisting of S. pneumoniae, H. influenzae, H. parainfluenzae, Burkholderia (Pseudomonas) cepacia and a mixture thereof, by administering a binding inhibiting effective amount of the compound of Formula I, ##STR1## where R.sub.1 is H, (.beta.-1)GalNAc--, SO.sub.3 B (where B is H or a cation) or a sialic acid of Formula II; ##STR2## where R.sub.6, R.sub.7, R.sub.8, and R.sub.10 are each independently H, C.sub.1-6 acyl, lactyl, C.sub.1-6 alkyl, sulfate, phosphate, anhydro, a sialic acid of Formula II, (.alpha.-1)Fuc, (.beta.-1)Glc or (.beta.-1)Gal;R.sub.9 is NH--C.sub.1-6 acyl, glycolylamido, amino or hydroxyl; andA is H or a cation;R.sub.2 is H or (.alpha.-1)Fuc--;R.sub.3 and R.sub.4 are each independently OH or NHAc;R.sub.5 is H, SO.sub.Type: GrantFiled: June 7, 1995Date of Patent: April 7, 1998Assignee: Neose Technologies, Inc.Inventors: Paul M. Simon, David Zopf, Roger A. Barthelson, Karl F. Johnson
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Patent number: 5620964Abstract: A composition for treating and/or inhibiting gastric and duodenal ulcers, comprising an oligosaccharide selected from the group consisting of Formula I ##STR1## or a mixture thereof; wherein:X is independently OH or NHAc;Y is independently H, or an amino acid or a peptide of 2-100, preferably 2-20, amino acids; andW, W', and W" are each independently H or ##STR2## where Z is independently H or a pharmaceutically acceptable cation; and P is independently H or ##STR3## where Z is defined as above; wherein at least one of W, W' or W" is an .alpha.-N-acetylneuraminic acid moiety, andwherein W' and W" are not simultaneously .alpha.-N-acetylneuraminic acid moietywith the proviso that the compound of Formula II is not NAN .alpha.(2.fwdarw.3)Gal .beta.1-4 Glu or NAN .alpha.(2.fwdarw.6)Gal .beta.1-4 Glu; and a kit for detecting the presence of Helicobacter pylori comprising at least one compound of the Formula I or II, are described.Type: GrantFiled: June 5, 1995Date of Patent: April 15, 1997Assignee: Neose Technologies, Inc.Inventors: Stephen Roth, Edward J. McGuire, Dennis H. Langer