Abstract: As a method for an efficient concentration of lipid components from food materials, a method for concentrating lipids contained in a crustacean, which comprises heating squeezed liquid prepared by squeezing the whole crustacean or a part thereof and separating the heated squeezed liquid into solids containing lipid components and liquid containing water-soluble components. Those are useful as the method by which lipids abundantly containing the phospholipid are prepared easily and at a low cost. Furthermore, the solids containing the lipids prepared by said method or a dried product thereof, lipids extracted therefrom and a composition abundantly containing the useful lipids derived from crustaceans are useful as materials for pharmaceuticals, ingredients for foods or feed, etc.

Abstract: To obtain a quality-meat-attached backbone part suitable as a material for minced fish the invention proposes cutting off a fin part and a kidney from a meat-attached backbone part obtained when filleting a fish into three pieces. The meat-attached backbone part is conveyed by a pair of upper and lower conveyor belts while being sandwiched and held between the belts. When the backbone part reaches a fin cutter arranged in a midstream of the conveyance, a belly part is pushed by a first pushing lever while a back part of the meat-attached backbone part is received and stopped by a receiving and stopping lever, and the back skin from a back fin to a tail fin is cut off by the fin cutter. Then, when the meat-attached backbone part reaches a kidney cutter, the belly part of the meat-attached backbone part is pushed by a second pushing lever to position the kidney-attached backbone section of the meat-attached backbone part at a cutting position by a kidney cutter.

Abstract: According to a method for producing a surimi by adding a protease inhibitor when producing a surimi from fish meat that contains kidney or tissue thereof, such as backbone meat, that is left over when using a filleting and deboning method to extract fillets from fish from which the head and internal organs have been removed, it is possible to produce a surimi and a paste product having stable quality from backbone meat obtained as a residue when producing fillets. The protease inhibitor is preferably a cysteine protease inhibitor.

Abstract: It is possible to provide an excellent salty taste enhancer that can compensate for insufficient salty taste when attempting to reduce the salt content of food. A salty taste enhancer obtained by adding a glutamic acid, containing dipeptide, specifically, Glu-Ala, Glu-Arg, Glu-Asn, Glu-Asp, Glu-Gln, Glu-Glu, Glu-Gly, Glu-His, Glu-Ile, Glu-Leu, Glu-Lys, Glu-Pro, Glu-Ser, Glu-Thr, Glu-Trp, Glu-Tyr, Glu-Val, Arg-Glu, Asn-Glu, Asp-Glu, Gln-Glu, His-Glu, Pro-Glu, Ser-Glu, Thr-Glu or Trp-Glu to an enzymatic decomposition product of a protein material and/or a basic amino acid, especially arginine. A method for producing these salty taste enhancers, a method for enhancing a salty taste by using these salty taste enhancers, and a food or drink that contains these salty taste enhancers.

Abstract: It is possible to provide an excellent salty taste enhancer is provided that can compensate for insufficient salty taste when attempting to reduce salt content in a food. A salty taste enhancer consisting of a mixture of an enzymatic decomposition product of an animal protein and an enzymatic decomposition product of a plant protein. A salty taste enhancer in which the enzymatic decomposition products are treated with a protein-hydrolyzing enzyme and/or a nucleic acid-hydrolyzing enzyme. A salty taste enhancer in which the animal protein is a fish or shellfish extract and the plant protein is a soy bean protein. A salty taste enhancer in which a basic amino acid, and especially arginine, is further added to the salty taste enhancer. A salty taste enhancer to which potassium chloride is further added. A salty taste enhancer obtained by rendering the salty taste enhancer mildly acidic.

Abstract: An antiparasitic agent for fish containing an inhibitor of folate synthesis and/or an inhibitor of folate activation as the active substance(s). A combination preparation composed of an inhibitor of folate synthesis and an inhibitor of folate activation is preferable, and a sulfonamide is preferable for the inhibitor of folate synthesis. A dihydrofolate reductase inhibitor, a folate antagonist, etc., can be used as the inhibitor of folate activation. The antiparasitic agent is able to exterminate fish parasites via oral administration. It is particularly effective against parasites belonging to the ciliate group among fish parasites.

Abstract: An object of the present invention is to provide a more simplified and more efficient process for producing a highly purified orange roughy oil having high storage stability. The present invention provides a process for producing a highly purified orange roughy oil substantially free of a polyunsaturated fatty acid ester having 4 to 6 double bonds, and having a saponification value of 98 to 113 and an iodine value of 73 to 89, comprising washing with an alkaline aqueous solution to remove a free fatty acid; hydrogenating with a catalyst to reduce a polyunsaturated fatty acid ester; and purifying by treatment with an adsorbent.

Abstract: Alcoholysis of oils and fats containing EPA and DHA is performed by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of a reaction additive such as magnesium oxide; then the glyceride fraction is separated; alcoholysis of the glyceride fraction is performed by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of a reaction additive such as magnesium oxide; and EPA-enriched oil and DHA-enriched oil are simultaneously obtained.

Abstract: The components which have suwari-inhibiting action of rice bran extracts is reduced or removed, enabling broader utilization of them for fish paste products. A rice bran extract in which components having specific physiologically active functions are singled out, and these components are reduced or removed. The components are suwari-inhibiting components. The rice bran extract is preferably a water extract. The components are reduced or removed by precipitation or dialysis. The precipitation is calcium precipitation. The suwari-inhibiting components are phytin and/or phytic acid. The rice bran extract can be used as a fish paste additive.

Abstract: Providing a frozen non-water-washed or water-washed at low level fish meat with suppressed freezing denaturation, from a fish species generally never used without water-wash. Biological iron, iron-containing substances and/or biological reducing substances in the fish meat are oxidized. A frozen non-water-washed or water-washed at low level fish meat made from minced fish meat or the dehydrated minced fish meat, where one or more cryoprotectants have been added and the decomposition of trimethylamine-N-oxide is suppressed through oxidation. Preferably, the fish meat is put in contact to oxygen under agitation of the fish meat, to thereby suppress trimethylamine-N-oxide decomposition, to which are added cryoprotectants for freezing. A frozen product of non-water-washed or water-washed at low level fish meat, where salts have been added for such agitation, followed by agitation. A fish paste product from the frozen product as the raw material.

Abstract: In order to examine whether or not a germ cell derived from a donor fish, which has been transplanted into a recipient fish of a different species by a surrogate fish technique, grows or matures in the gonad of the recipient fish, it is necessary to use, as an indicator, a trait that is specifically expressed in the germ cell and can be used to distinguish the recipient fish from the donor fish. Vasa gene, which is a germ cell-specific gene, is specific to a primordial germ cell and a spermatogonium/an oogonium, and it is not expressed in a somatic cell. In the present invention, the Vasa gene sequences of a tuna, a chub mackerel, a spotted mackerel, an eastern little tuna, and a drumfish are determined, and the expression of such gene is used as a marker for a germ cell. In addition, according to the present invention, it is possible to specifically detect only a tuna Vasa gene in Vasa gene sequences that are highly conserved in fishes, without sequencing.

Abstract: A process for producing a crude oil having lowered unsaponifiable matter content and/or ester-type sterol content and comprising a highly unsaturated fatty acid as a constituent fatty acid, characterized in that a microorganism capable of producing a fat or oil comprising an unsaturated fatty acid as a constituent fatty acid is cultured in a medium containing a nitrogen source concentration of 2 to 15% within a culture tank equipped with an agitation impeller satisfying the requirement that the ratio of the diameter of agitation impeller (=d) to the inner diameter of the culture tank (=D) is d/D=0.30 to 0.6.

Abstract: [PROBLEMS] To provide a fat-and-oil in which the content of docosahexaenoic acid is increased. [MEANS FOR SOLVING PROBLEMS] A process for producing highly unsaturated fatty acids comprising culturing a stramenopile capable of producing highly unsaturated fatty acids in a culture medium containing an inhibitor for fatty acid desaturases; fats-and-oils in which the content of highly unsaturated fatty acids, particularly docosahexaenoic acid produced by the relevant method, is increased. A method for enhancing the productivity of highly unsaturated fatty acids in stramenopiles, comprising culturing a stramenopile in a culture medium containing an inhibitor for fatty acid desaturases; stramenopiles having the enhanced productivity of highly unsaturated fatty acids, generated by the relevant method.

Abstract: The invention provides dairy products containing fish-oil-originated EPA and/or DHA and having oxidation and emulsification stability. In particular, acidified milk containing EPA and/or DHA is provided which has oxidation and emulsification stability. The acidified milk is milk acidified by addition of an acid, fermented milk, or acidified milk containing any of the milk acidified by addition of an acid and the fermented milk. The acidified milk contains EPA and/or DHA as a fish oil, preferably a purified fish oil or a fish oil containing EPA and/or DHA in adjusted amount. The acidified milk is produced through an emulsification process. Preferably, the emulsification is performed after a fermentation process of the acidified milk process through a two-stage emulsification process. A food product containing the acidified milk of the invention is also provided.

Abstract: The present invention provides a process for concentrated PUFA oil, characterized in that alcoholysis reaction using lipase is carried out in the presence of a small amount of water and at least one compound as an additive selected from magnesium oxide, magnesium hydroxide, calcium oxide and calcium hydroxide, and then separation is conducted to obtain a glyceride fraction.

Abstract: A crustacean-derived protein, as measured by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, displays a non-reduced band of a molecular weight of about 200,000, displays reduced-form bands at about 86,000 and 90,000 molecular weights, and has N-terminal amino acids as indicated by SEQ ID No. 1 or SEQ ID No. 2. This protein is contained in large amounts in the crustacean shells that are largely discarded except for use for meal, chitin, and chitosan raw material. Thus large volume preparation, as well as inexpensive production, is possible. Moreover, application by addition to foods is easy since this is a food-derived protein. Since the protein of the present invention has ice nucleation activity and recrystallization inhibition activity for ice crystals, wide use is possible with the object of quality maintenance and the like of frozen food and the like.

Abstract: A crustacean-derived protein having antifreeze activity which under reduced conditions has a molecular weight, as measured by sodium dodecyl sulfate (SDS)—polyacrylamide gel electrophoresis, of about 37,000, about 16,000, and/or about 15,400; and the N-terminal amino acids are indicated by SEQ ID No. 1 or SEQ ID No. 2. Since this protein is excellent in comparison to the conventional antifreeze proteins from the standpoints of safety, activity, production rate, cost, and the like, use is possible in a wide range of fields, beginning with foods.

Abstract: A method for detecting foreign matter such as a parasite, in particular, to provide a method for detecting parasitic on meat. A method for detecting foreign matter includes detecting fluorescence excited by irradiating the foreign matter such as a parasite with visible light. The method is suitable for detecting a meat parasite. A method for detecting a parasite includes selectively detecting fluorescence emitted from a parasite with a filter that does not transmit excitation light but that transmits fluorescence emitted from the parasite. An apparatus for detecting a parasite includes a light source that illuminates a specimen with a visible light having a predetermined wavelength, a filter that absorbs diffuse reflection of excitation light, a camera that shoots fluorescence from the specimen, an image processing unit, and a monitor.

Abstract: Fish and shellfish meat whose color tone is well preserved, particularly no Listeria contamination and in which discoloration is effectively prevented, and smoked products prepared from such fish and shellfish meat are provided. Fish meat which is no Listeria contamination and in which discoloration is prevented can be obtained by treating the meat with a cultivation medium used for cultivating lactic acid bacteria. Specifically, the meat is immersed preferably in a sterilized or disinfected cultivation medium used for cultivating a lactic acid bacterium having antibacterial and anti-oxidative effects, or two lactic acid bacteria in combination, one having antibacterial effect and the other anti-oxidative effect. Combinational use of Lactobacillus sake D-1001 (FERM P-11708 or IPOD FERM BP-08544) and Lactococcus lactis IFO 12007 is preferred. The fish meat is preferably derived from salmon, trout or cod, and the inventive method is particularly effective for fish meat containing astaxanthin.

Abstract: A green soybean having a deep green color, prevented from discoloring, and being adaptable for chilled distribution is provided as a cooked and frozen product. The green soybean is hard to discolor and is frozen after cooking. The green color is retained even in a condition exposed to illumination of light. The green soybean contains chlorophyll a of not less than 3.8 g/100 g wet weight. A material for suppressing decomposition of chlorophyll, preferably an anti-oxidation material, e.g., ?-carotene, is accumulated in tissues of the green soybean. Preferably, ?-carotene of not less than 750 ?g/100 g wet weight is accumulated in the green soybean. The invention also provides a green soybean thawed from the frozen green soybean. The thawed green soybean is adaptable for chilled distribution.