Abstract: The present invention provides an approach for the determination of activation state of a plurality of discrete cell populations and/or the state of one or more cellular networks in an individual.
Abstract: This invention is directed to methods and compositions for diagnosis, prognosis and for determining methods of treatment. The physiological status of a cell present in a sample (e.g. clinical sample) can be used in diagnosis or prognosis of a condition (e.g. Chronic Lymphocytic Leukemia), in patient selection for therapy, to monitor treatment and to modify or optimize therapeutic regimens.
Type:
Application
Filed:
May 20, 2010
Publication date:
November 25, 2010
Applicant:
Nodality, Inc.
Inventors:
Wendy J. Fantl, Alessandra Cesano, Erik Evensen, Adam Palazzo
Abstract: Fluorescently labeled microsphere calibration and quality control particles for use in establishing standardized fluorescence detector setup, monitoring daily QC of flow cytometers and quantitative calibration of the fluorescence detectors in traceable units of measure (molecules equivalent soluble fluorescence; MESF).
Abstract: As disclosed herein, the present invention provides for kits and a composition for diagnosis, prognosis, drug discovery, drug development, and patient stratification. The kits can comprise a plurality of binding elements for cell surface markers, and a plurality of binding elements for state-specific intracellular markers. The kits can further comprise a plurality of modulators directed for the particular cell function or signaling pathways. The kits can further include fixatives, permeabilizing agent, buffers, containers, instructions, and software for data analysis/compilation.
Type:
Application
Filed:
March 23, 2010
Publication date:
September 23, 2010
Applicant:
Nodality, Inc.
Inventors:
David Soper, David Rosen, Todd Covey, Ying-Wen Huang, Wendy Fantl, Ralph Lin
Abstract: An embodiment of the present invention is a method for measuring the post translational states and expression levels of proteins in the PI3K and/or mTor for use in diagnosis, prognosis and drug screening applications.
Abstract: An embodiment of the present invention is a method for subjecting a hematopoetic cell to a JAK/STAT inhibitor, determining the activity of gain-of-function mutations of a Jak family kinase, determining the expression levels and activity of JAK/STAT regulatory proteins, correlating the expression levels and the activity of JAK/STAT regulatory proteins with the activity of gain-of-function mutations of a Jak family kinase and with a response to the JAK/STAT inhibitor, and then classifying the cells. A further embodiment of the invention includes determining the clinical outcome based on the cell classification, determining a method of treatment, determining dosing and scheduling of at least one of the JAK/STAT inhibitors or other compounds.
Type:
Application
Filed:
January 14, 2010
Publication date:
August 19, 2010
Applicant:
Nodality, Inc., A Delaware Corporation
Inventors:
Wendy J. Fantl, Alessandra Casano, Todd Covey
Abstract: The invention provides methods, kits, devices and compositions for detecting one or more target analytes. In some embodiments, the invention provides binding elements and labeling elements capable of being joined through a plurality of joining elements.
Abstract: The invention provides systems, compositions, kits and methods for automated processing of biological samples and analysis using a flow cytometer.
Type:
Application
Filed:
October 27, 2009
Publication date:
April 29, 2010
Applicant:
Nodality, Inc. a Delaware Corporation
Inventors:
Todd Covey, Adam Palazzo, Norman Purvis, James Cordiero, David Rosen
Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.
Type:
Application
Filed:
July 10, 2009
Publication date:
January 14, 2010
Applicant:
Nodality, Inc.
Inventors:
Wendy J. Fantl, David B. Rosen, Alessandra Cesano, Santosh K. Putta, Garry Nolan, Aileen Cohen, Erik Evensen
Abstract: Methods of determining status of an individual based on the use of biological specimen and analysis of reference population of cells are described.
Type:
Application
Filed:
May 22, 2009
Publication date:
November 26, 2009
Applicant:
Nodality, Inc.
Inventors:
Aileen Cohen, Helen Francis-Lang, Malcolm Francis-Lang
Abstract: This invention is directed to methods and compositions for diagnosis, prognosis and for determining methods of treatment. The physiological status of cells present in a sample (e.g. clinical sample) can be used in diagnosis or prognosis of a condition (e.g. Chronic Lymphocytic Leukemia), in patient selection for therapy, to monitor treatment and to modify or optimize therapeutic regimens. The physiological status of a cell can be determined by comparing the intracellular status of one or more activation elements (e.g. the phosphorylation status of a signaling molecule) in a cell (e.g. a cancer cell) to that of another cell (e.g. a normal cell). The physiological status of a cell can be further classified by adding one or more modulators (e.g. an inhibitor or activator) to the cell in question. In some embodiments, the invention is directed to methods of determining a phenotypic profile of a population of cells.
Type:
Application
Filed:
August 21, 2008
Publication date:
April 16, 2009
Applicant:
Nodality, Inc.
Inventors:
Wendy J. Fantl, Santosh K. Putta, Omar D. Perez, Helen L. Francis-Lang, Aileen C. Cohen