Abstract: The invention relates to a method for purifying a first fatty acid, in particular a first polyunsaturated fatty acid, using an initial mixture further comprising at least one second fatty acid and a third fatty acid, with the method comprising at least: a first step of chromatographic separation in liquid phase, using the initial mixture, making it possible to recover on the one hand a first flow enriched with a first fatty acid and on the other hand a flow enriched with a second fatty acid; a second step of chromatographic separation in liquid phase, using the first flow enriched with a first fatty acid, making it possible to recover on the one hand a second flow enriched with a first fatty acid and on the other hand a flow enriched with a third fatty acid, with the second step of chromatographic separation being carried out in a static bed chromatographic separation unit.

Abstract: The invention concerns a method for separating fractions of a mixture to be separated, in a device (1) comprising: several chromatography columns (21, 22, 23,) mounted in series; an open separation loop comprising in input a point for injecting (6) eluent in one of the columns and in output a point for drawing (8) a fraction of the mixture. The method includes the following steps: batch injection of the mixture to be separated in the open separation loop (4); collecting at least two fractions; offsetting at least one column of the eluent injecting and fraction drawing points (6, 8) of the separation loop (4). The invention also concerns a device for separating fractions of a mixture to be separated.

Abstract: A method for purification of a first polyunsaturated fatty acid from an initial mixture is provided. The initial mixture comprises at least one second fatty acid in addition to the first polyunsaturated fatty acid. The method may comprise separating the first polyunsaturated fatty acid and the second fatty acid in a liquid phase in at least one step of a chromatographic separation process, so as to recover, on the one hand, a stream enriched in the first polyunsaturated fatty acid, and on the other hand, a stream enriched in the second fatty acid. The method may further comprise a step of processing the stream enriched in the first polyunsaturated fatty acid to decrease the peroxide value and/or anisidine value of this stream. The disclosure also provides systems or devices suitably adapted for the implementation of such methods.

Abstract: A method for purification of a first polyunsaturated fatty acid from an initial mixture is provided where the initial mixture comprises at least one second fatty acid in addition to the first polyunsaturated fatty acid. The method may comprise: at least one step of chromatographic separation in liquid phase of the first polyunsaturated fatty acid and the second fatty acid, so as to recover, on the one hand, a stream enriched in the first polyunsaturated fatty acid, and on the other hand, a stream enriched in the second fatty acid; a step of processing the stream enriched in the first polyunsaturated fatty acid, leading to a decrease in the peroxide value and/or anisidine value of this stream. The disclosure also provides a system suitably adapted for the implementation of this method.