Abstract: A method and device for aggregating algae in an aqueous solution is disclosed. The method can include providing an algae feed comprising a liquid and algae dispersed therein. The algae feed can be aggregated by applying a nanosecond pulsed electric field to the algae feed. The nanosecond pulsed electric field can include a plurality of electric pulses having a pulse duration ranging from 1 to 1,000 nanoseconds. The method can also include separating an aggregated algae stream from the algae feed and feeding the aggregated algae stream to a lipid extraction operation.

Abstract: A plasma reactor is provided, which includes a discharge chamber with dimensional characteristics and configuration of dielectric and electrodes so as to enhance efficiency based on the characteristics of the corona discharge streamers generated. Upon application of a pulsed high voltage potential, the discharge chamber enables formation of plasma where surface streamers play a greater role in the overall energy density of the discharge chamber than gas streamers. The formation of gas streamers is constrained. Because surface streamers have a higher energy density, the present invention is able to achieve improved energy efficiency while preserving effectiveness for gas treatment.

Abstract: A method of inducing local hyperthermia in patient tissue is provided. The method includes generating ultra-wide-band radiation, conveying the ultra-wideband radiation to a focusing element, and focusing the ultra-wideband radiation on a target with the focusing element. A system for inducing local hyperthermia in patient tissue is also provided. The system includes a power source for generating ultra-wideband radiation, and a focusing element for focusing the ultra-wideband radiation on a target.

Abstract: A pulse delivery system for producing a spark in tissue is disclosed. The system can include at least two electrodes having sharp surface features, where the at least two electrodes are electrically isolated from one another. The sharp features of the electrodes can be separated by a distance of about 2 to 20 mm. The system can also include a pulse generator for delivering pulsed voltage differences between the at least two electrodes. The pulsed voltage differences can producing a voltage difference ranging from about 2.5 kV to about 35 kV for a duration of about 50 ns to 3 ?s. The system can include a controller for determining whether the plurality of pulsed voltage differences produced a spark between the at least two electrodes. A method of producing a spark in tissue for both therapeutic and cosmetic, i.e., non-therapeutic, applications is also disclosed.

Abstract: An imaging and recordation system is provided. The system includes a high-power, focusing antenna for illuminating biological tissue. The system further includes a power source for powering the antenna. The system further includes a data acquisition module, for recording the dielectric properties of tissues illuminated by the high-power, focusing antenna. The system illuminates the tissues using ultrashort electrical pulses.

Abstract: A system for measuring normal load and deflection of a material that includes a portable load deflection device and a method of using the same is disclosed. The system includes a support arm having a first end and a second end opposite said first end. The first end can be attached to a base surface. The support arm can provides at least three degrees of freedom of movement and includes at least one sensor for determining a position of the second end. A uniaxial load cell can be operably attached to the second end. The system can also include a processing system operable to receive position data and corresponding force data when the support arm is used to apply a non-normal compression force to the test surface. The data can be used to calculate normal force versus deflection data for the test surface.

Abstract: A plasma generator, comprising a dielectric tube having a first end and a second end, wherein the first end is sealed, but for a gas inlet; at least one first dielectric disk located within the dielectric tube, wherein the first dielectric disk includes at least one first dielectric aperture formed therein; a first ring electrode that at least partially surrounds the at least one first dielectric aperture and is electrically coupled to a power supply; at least one second dielectric disk located proximate the second end of the dielectric tube, wherein the second dielectric disk includes at least one second dielectric aperture formed therein; and a second ring electrode that at least partially surrounds the at least one second dielectric aperture and is electrically coupled to the power supply. During use, the plasma generator produces at least one plasma plume that is launched into open air.

Abstract: A raceway for cultivating algae in a liquid medium, comprising one or more precipitators positioned along the raceway, and at which precipitators the algal biomass produced in the raceway is precipitated from the liquid medium and concentrated.

Abstract: Methods for inducing calcium mobilization in cells through the application of nanosecond pulsed electric fields (“nsPEFs”) are provided. The invention also provides a method of increasing intracellular calcium in cells through the application of nsPEFs. In one embodiment of the invention, the cells are human platelets, whereby activation and aggregation of the platelets is induced. Methods for treating an injury, trauma, or loss of blood in a subject, through the application of nsPEFs are also provided.

Abstract: Disclosed is the direct conversion process for producing biodiesel from a biomass. The direct conversion process for producing biodiesel from a biomass comprises reacting a feed stock comprising a biomass and an alkylation reagent in a substantially oxygen free environment at a temperature sufficient to hydrolyze one or more lipid glycerides in the biomass and alkylated one or more fatty acids in the reaction. The process may comprise reacting a feed stock comprising an algal biomass and tetramethylammonium hydroxide in a substantially oxygen free environment at a temperature between 250° C. and 500° C. The direct conversion process for producing biodiesel may further comprise reacting oil containing lipid glycerides with an alkylation reagent at a sufficient temperature to esterify the oil. The fatty acid alkyl esters produced from the reacted feed stock are recovered. The recovered fatty acid alkyl esters, as an essential component of biodiesel, may be formulated into biodiesel.

Abstract: Method and apparatus for cellular and intracellular manipulation of cell functions with ultrashort electrical pulses and for targeted delivery of the electrical pulses into cell cultures, patients, and tissues.

Abstract: A new image enhancement process based on an integrated neighborhood dependent nonlinear approach for color images captured in various environments such extremely low lighting, fog or underwater. The new process is a combination of two independent processes: luminance enhancement and contrast enhancement. The luminance enhancement, also regarded as a process of dynamic range compression, is essentially an intensity transformation based on a specifically designed nonlinear transfer function, which can largely increase the luminance for dark regions of the image but only slightly change the luminance for the bright regions of the image. The contrast enhancement transforms each pixel's intensity based on the relationship between the pixel and its surrounding pixels. The output of the contrast enhancement is a power function of the luminance of the input image.

Abstract: A system for producing maximal, but reversible bioeffects, for neuromuscular disruption. Methods for facilitation of neuromuscular functions in diseases and conditions of decreased excitability are provided.

Abstract: The present invention provides a system and method for simulating medical conditions to facilitate medical training, that utilizes a stethoscope comprising an earpiece and a headpiece and configured to transmit information indicative of a medical condition, and a triggering device configured to activate the transmission of the information.

Abstract: An intracellular electro-manipulation apparatus for delivery an electric field pulse to a target of one or more biological cells is provided. The apparatus includes a pulse generator that generates an ultrashort electric field pulse, and a pulse delivery system that directs the ultrashort electric field pulse to the target. The apparatus can include a reflected-signal impeder connected between the pulse generator and the pulse delivery system to impede reflection of a signal to the pulse generator when impedance mismatching between the pulse delivery system and pulse generator occurs. Alternatively, or additionally, the apparatus can include a current limiter connected between the pulse generator and the pulse delivery system to limit current between the pulse generator and the pulse delivery system when a high-conductivity condition occurs in the target.

Abstract: A method of characterizing and enhancing the pixels in an image, which is captured at all lighting conditions. The method enable dynamically enhancement of images captured at extremely low lighting conditions. Each pixel of the image, represented by I SUB (x, y) where x is the horizontal axis coordinate value and y is the vertical axis coordinate value, contains three color-components. Each pixel is characterized by defining a relationship between the three color-components of that pixel in its initial state. A nonlinear expansion function, which adaptively adjusts the expansion rate with respect to the statistical property of the original image, is used for enhancing the brightness of the image so that the poorly lighted pixels are enhanced in intensity more as compared to the amount of enhancement for the brighter pixels. The color property of each pixel in the enhanced image is then restored using the characteristic features previously defined by the relationship of the color components.

Abstract: A plasma reactor is provided, which includes a discharge chamber with dimensional characteristics and configuration of dielectric and electrodes so as to enhance efficiency based on the characteristics of the corona discharge streamers generated. Upon application of a pulsed high voltage potential, the discharge chamber enables formation of plasma where surface streamers play a greater role in the overall energy density of the discharge chamber than gas streamers. The formation of gas streamers is constrained. Because surface streamers have a higher energy density, the present invention is able to achieve improved energy efficiency while preserving effectiveness for gas treatment.

Abstract: A novel plasma reactor is provided that includes a discharge chamber with dimensional characteristics and configuration of dielectric and electrodes that optimize efficiency based on the characteristics of the corona discharge streamers generated. Upon application of a pulsed high voltage potential, the discharge chamber enables formation of plasma where surface streamers play a greater role in the overall energy density of the discharge chamber than gas streamers. The formation of gas streamers is constrained. Because surface streamers have a higher energy density, the present invention is able to achieve improved energy efficiency while preserving effectiveness for gas treatment.

Abstract: A method and apparatus are provided for delivering an agent into a cell through the application of nanosecond pulse electric fields (“nsPEF's”). The method includes circuitry for delivery of an agent into a cell via known methods followed by the application of nanosecond pulse electric fields to said cell in order to facilitate entry of the agent into the nucleus of the cell. In a preferred embodiment, the present invention is directed to a method of enhancing gene expression in a cell comprising the application of nanosecond pulse electric fields to said cell. An apparatus for generating long and short pulses according to the present invention is also provided. The apparatus includes a pulse generator capable of producing a first pulse having a long duration and low voltage amplitude and a second pulse having a short duration and high voltage amplitude.

Abstract: An electrodeless excimer UV lamp, comprising an enclosed chamber with a gas sealed within the enclosed chamber, wherein the gas is capable of being used to generate a plasma discharge, a first electrode wrapped around the outer surface of the chamber at a first location, a second electrode wrapped around the outer surface of the chamber at second location, and a power supply configured to apply a voltage to the first electrode and the second electrode. During operation of the UV lamp, a plasma discharge is generated by applying a voltage to the electrodes wrapped around the outer surface of the chamber to ignite the gas or gas mixture inside the chamber and generate a plasma discharge within the chamber, such that a specific wavelength of UV radiation will be generated by the particular gas within the chamber.