Abstract: The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.
Type:
Application
Filed:
August 9, 2021
Publication date:
April 27, 2023
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, Daniel John Turner, James White
Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
Type:
Application
Filed:
April 30, 2021
Publication date:
January 12, 2023
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
Type:
Application
Filed:
October 13, 2021
Publication date:
May 5, 2022
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
Type:
Application
Filed:
October 8, 2021
Publication date:
April 28, 2022
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, James Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
Abstract: The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.
Type:
Application
Filed:
August 9, 2021
Publication date:
March 31, 2022
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, Daniel John Turner, James White
Abstract: The invention relates to a new method of characterising two or more target polynucleotides using a pore. The method involves sequentially attaching to a first polynucleotide one or more subsequent polynucleotides to form a concatenated polynucleotide.
Type:
Application
Filed:
July 19, 2021
Publication date:
March 24, 2022
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Andrew John Heron, Daniel Ryan Garalde, James White
Abstract: A target polynucleotide is expanded. In respect of each nucleotide in the target polynucleotide, the target polynucleotide comprises clock nucleotides and at least one signal nucleotide in a predetermined order. The clock nucleotides have a predetermined sequence common to each nucleotide in the target polynucleotide. The at least one signal nucleotide is characteristic of the identity of the respective nucleotide in the target polynucleotide. During translocation of the expanded polynucleotide through a nanopore, electrical measurements dependent on the polynucleotide within the pore are made, to derive an analysis signal. Clock signals derived from the clock nucleotides are identified. Relative to the positions of the identified clock signals, nucleotide signals derived from the least one signal nucleotide are derived to analyse the target polynucleotide.
Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
Type:
Application
Filed:
June 23, 2021
Publication date:
March 3, 2022
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Clarke
Abstract: An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.
Type:
Application
Filed:
July 1, 2021
Publication date:
January 27, 2022
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Jason Robert Hyde, Pedro Miguel Ortiz Bahamon, Clive Gavin Brown, Andrew John, Paul Raymond Mackett
Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
Type:
Application
Filed:
April 30, 2021
Publication date:
December 23, 2021
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
Type:
Grant
Filed:
October 6, 2015
Date of Patent:
November 23, 2021
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
Type:
Grant
Filed:
May 31, 2019
Date of Patent:
November 9, 2021
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
Abstract: An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.
Type:
Grant
Filed:
October 25, 2018
Date of Patent:
November 9, 2021
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, James Peter Willcocks
Abstract: The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.
Type:
Grant
Filed:
July 18, 2013
Date of Patent:
October 26, 2021
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
James White, Ruth Moysey, Mihaela Misca
Abstract: The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.
Type:
Grant
Filed:
October 17, 2014
Date of Patent:
September 7, 2021
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, Daniel John Turner, James White