Patents Assigned to Oxford Nanopore Technologies Ltd.
  • Publication number: 20220154269
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.
    Type: Application
    Filed: March 8, 2021
    Publication date: May 19, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Publication number: 20220135956
    Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
    Type: Application
    Filed: October 13, 2021
    Publication date: May 5, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
  • Publication number: 20220127669
    Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
    Type: Application
    Filed: October 8, 2021
    Publication date: April 28, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Publication number: 20220098657
    Abstract: The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.
    Type: Application
    Filed: August 9, 2021
    Publication date: March 31, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, Daniel John Turner, James White
  • Publication number: 20220090192
    Abstract: The invention relates to a new method of characterising two or more target polynucleotides using a pore. The method involves sequentially attaching to a first polynucleotide one or more subsequent polynucleotides to form a concatenated polynucleotide.
    Type: Application
    Filed: July 19, 2021
    Publication date: March 24, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Andrew John Heron, Daniel Ryan Garalde, James White
  • Publication number: 20220064230
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.
    Type: Application
    Filed: July 26, 2021
    Publication date: March 3, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Mark Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
  • Publication number: 20220064724
    Abstract: A target polynucleotide is expanded. In respect of each nucleotide in the target polynucleotide, the target polynucleotide comprises clock nucleotides and at least one signal nucleotide in a predetermined order. The clock nucleotides have a predetermined sequence common to each nucleotide in the target polynucleotide. The at least one signal nucleotide is characteristic of the identity of the respective nucleotide in the target polynucleotide. During translocation of the expanded polynucleotide through a nanopore, electrical measurements dependent on the polynucleotide within the pore are made, to derive an analysis signal. Clock signals derived from the clock nucleotides are identified. Relative to the positions of the identified clock signals, nucleotide signals derived from the least one signal nucleotide are derived to analyse the target polynucleotide.
    Type: Application
    Filed: July 7, 2021
    Publication date: March 3, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Stuart William Reid, Gavin Harper
  • Publication number: 20220064722
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Application
    Filed: June 23, 2021
    Publication date: March 3, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Clarke
  • Patent number: 11236385
    Abstract: Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid.
    Type: Grant
    Filed: May 21, 2019
    Date of Patent: February 1, 2022
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Marion Louise Crawford, James White
  • Publication number: 20220023819
    Abstract: An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.
    Type: Application
    Filed: July 1, 2021
    Publication date: January 27, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Jason Robert Hyde, Pedro Miguel Ortiz Bahamon, Clive Gavin Brown, Andrew John, Paul Raymond Mackett
  • Publication number: 20210395811
    Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
    Type: Application
    Filed: April 30, 2021
    Publication date: December 23, 2021
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
  • Patent number: 11180741
    Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: November 23, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
  • Patent number: 11169113
    Abstract: An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.
    Type: Grant
    Filed: October 25, 2018
    Date of Patent: November 9, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Peter Willcocks
  • Patent number: 11168363
    Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
    Type: Grant
    Filed: May 31, 2019
    Date of Patent: November 9, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Patent number: 11155860
    Abstract: The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: October 26, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James White, Ruth Moysey, Mihaela Misca
  • Publication number: 20210317520
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: March 29, 2021
    Publication date: October 14, 2021
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Publication number: 20210292376
    Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: March 8, 2021
    Publication date: September 23, 2021
    Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Patent number: 11111532
    Abstract: The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.
    Type: Grant
    Filed: October 17, 2014
    Date of Patent: September 7, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, Daniel John Turner, James White
  • Patent number: 11111524
    Abstract: Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3? and/or 5? ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3? and/or 5? end sequences; and/or the flanking sequence.
    Type: Grant
    Filed: November 22, 2019
    Date of Patent: September 7, 2021
    Assignee: OXFORD NANOPORE TECHNOLOGIES, LTD.
    Inventors: Robert Osborne, Esther Musgrave-Brown
  • Publication number: 20210269872
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.
    Type: Application
    Filed: March 8, 2021
    Publication date: September 2, 2021
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace