Abstract: The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.
Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
Type:
Grant
Filed:
September 10, 2014
Date of Patent:
July 28, 2020
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Mark John Bruce, Andrew John Heron, Ruth Moysey, Szabolcs Soeroes, Elizabeth Jayne Wallace, James White
Abstract: The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.
Type:
Grant
Filed:
October 6, 2015
Date of Patent:
July 28, 2020
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, Daniel Ryan Garalde, Andrew John Heron, Daniel John Turner, James White
Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein.
Type:
Application
Filed:
September 17, 2019
Publication date:
July 16, 2020
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Lakmal Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White, Andrew John Heron, Mark John Bruce, Christopher Peter Youd, Rebecca Victoria Bowen
Abstract: Analysis Of A Polymer A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.
Type:
Grant
Filed:
October 16, 2015
Date of Patent:
June 23, 2020
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Stuart William Reid, Gavin Harper, Clive Gavin Brown, Daniel John Turner, Andrew John Heron, Christopher James Wright
Abstract: Techniques for estimating a polymer sequence of a polymer based on a signal produced as a result of translocation of the polymer through a nanopore are described. The techniques may analyze portions of the signal to estimate whether there was a transition in the polymer sequence during each respective portion and which units of the sequence the transition was between. The techniques may comprise operation of one or more neural networks into which data from the signal may be input. The techniques may include generating a plurality of weights for a portion of the signal, wherein each weight is associated with a transition between labeled units of the polymer. The weights may be indicative of a likelihood that a transition occurred between a first of the labeled units to a second of the labeled units within the portion of the signal.
Abstract: The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.
Type:
Grant
Filed:
February 19, 2015
Date of Patent:
June 2, 2020
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
James Anthony Clarke, Marion Louise Crawford, James White
Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterization using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterized.
Abstract: A pump for use in low-profile applications comprises a barrel for holding fluid; and a piston that converts a rotational driving force into a longitudinal driving motion within the barrel. The pump provides space saving advantages by reducing the need for external equipment and mechanisms around the pump for providing actuation or moving the actuating mechanism.
Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
Type:
Grant
Filed:
April 3, 2018
Date of Patent:
March 24, 2020
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
Abstract: The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also related to new methods of characterising target polynucleotides.
Type:
Application
Filed:
August 14, 2019
Publication date:
March 19, 2020
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Andrew John Heron, Clive Gavin Brown, Rebecca Victoria Bowen, James White, Daniel John Turner, Joseph Hargreaves Lloyd, Christopher Peter Youd
Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
Type:
Application
Filed:
September 19, 2019
Publication date:
February 27, 2020
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.
Abstract: A detachable electrical device can be formed from a kit comprising a pair of component parts adapted for connection to each other, wherein the connected components of the device may be subsequently disconnected, comprising: an array of electrical connectors, each electrical connector comprising an electrically conductive liquid; and an array of electrodes; wherein the arrays can be brought into contact with each other so as to provide a plurality of electrical connections between the electrically conductive liquid of the array of electrical connectors and the electrodes of the array of electrodes, and wherein the electrical connections may be subsequently broken by detaching the electrically conductive liquid from the electrodes of the array.
Type:
Grant
Filed:
October 15, 2015
Date of Patent:
February 4, 2020
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, Jason Robert Hyde, Mark David Jackson, Paul Raymond Mackett, Jonathan Edward McKendry, Richard Kenneth John Wiltshire
Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
Type:
Application
Filed:
May 31, 2019
Publication date:
January 23, 2020
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
Abstract: The invention relates to a new method of characterising two or more target polynucleotides using a pore. The method involves sequentially attaching to a first polynucleotide one or more subsequent polynucleotides to form a concatenated polynucleotide.
Type:
Application
Filed:
May 25, 2017
Publication date:
January 9, 2020
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Andrew John Heron, Daniel Ryan Garalde, James White Oxford
Abstract: The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.
Abstract: Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3? and/or 5? ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3? and/or 5? end sequences; and/or the flanking sequence.