Abstract: The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled from the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.
Type:
Application
Filed:
March 31, 2015
Publication date:
January 26, 2017
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
James Anthony Clarke, Marion Louise Crawford, James White
Abstract: The invention relates to a method for modifying a template polynucleotide for characterization, especially for nanopore sequencing. The method produces a modified polynucleotide which is complementary to the template polynucleotide at some positions and which contains universal or abasic nucleotides at the other, and in some instances predicable, positions. The resulting modified polynucleotide can then be characterized.
Type:
Grant
Filed:
September 6, 2013
Date of Patent:
January 24, 2017
Assignee:
Oxford Nanopore Technologies Ltd.
Inventors:
Daniel John Turner, Clive Gavin Brown, Stuart William Reid, James Anthony Clarke, James White, David Jackson Stoddart
Abstract: A pump for use in low-profile applications comprises a barrel for holding fluid; and a piston that converts a rotational driving force into a longitudinal driving motion within the barrel. The pump provides space saving advantages by reducing the need for external equipment and mechanisms around the pump for providing actuation or moving the actuating mechanism.
Abstract: The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterising target polynucleotides using helicases.
Type:
Application
Filed:
September 10, 2014
Publication date:
January 5, 2017
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Rebecca Victoria Bowen, Clive Gavin Brown, Mark Bruce, Andrew John Heron, Elizabeth Jayne Wallace, James White, Joseph Hargreaves Lloyd, David Antoni Alves, Domenico Caprotti, Lakmal Jayasinghe, Luke McNeill, John Milton, Antonino Puglisi, Szabolcs Soeroes
Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
Type:
Application
Filed:
August 24, 2016
Publication date:
December 15, 2016
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
Type:
Application
Filed:
June 10, 2016
Publication date:
September 29, 2016
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
Type:
Application
Filed:
September 10, 2014
Publication date:
September 8, 2016
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Mark Bruce, Andrew John Heron, Ruth Moysey, Szabolcs Soeroes, Elizabeth Jayne Wallace, James White
Abstract: The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.
Type:
Application
Filed:
October 17, 2014
Publication date:
September 1, 2016
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Clive Gavin Brown, Daniel John, James White
Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.
Abstract: The invention provides a method of forming a membrane between a first volume of polar medium and a second volume of polar medium, which method comprises: (a) providing a first volume comprising polar medium and a second volume comprising polar medium which are separated from one another by an apolar medium, wherein at least one of said first and second volumes comprises a layer comprising amphipathic molecules, at the interface between the polar medium and the apolar medium, wherein each of the amphipathic molecules comprises a first outer hydrophilic group, a hydrophobic core group, and a second outer hydrophilic group, wherein each of the first and second outer hydrophilic groups is linked to the hydrophobic core group; and (b) causing the first and second volumes to come into contact with one another to form a membrane comprising said amphipathic molecules between the first and second volumes.
Type:
Application
Filed:
October 23, 2013
Publication date:
October 8, 2015
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Andrew John Heron, Jason Robert Hyde, Clive Gavin Brown
Abstract: An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.
Type:
Application
Filed:
October 23, 2013
Publication date:
September 24, 2015
Applicant:
Oxford Nanopore Technologies Ltd.
Inventors:
Jason Robert Hyde, Pedro Miguel Ortiz Bahamon, Clive Gavin Brown, Andrew John Heron, Paul Raymond Mackett