Patents Assigned to Oxford Nanopore Technologies Ltd.
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Publication number: 20180230526Abstract: The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: ApplicationFiled: September 14, 2017Publication date: August 16, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Andrew John Heron, James Anthony Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, Mihaela Misca
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Publication number: 20180216175Abstract: The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.Type: ApplicationFiled: August 11, 2017Publication date: August 2, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Ruth Moysey, Andrew John Heron
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Publication number: 20180208632Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.Type: ApplicationFiled: August 31, 2017Publication date: July 26, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Mark Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Publication number: 20180179500Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: ApplicationFiled: October 6, 2015Publication date: June 28, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
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Patent number: 9995728Abstract: The invention relates to a new method of determining in a sample the presence, absence or concentration of one or more target analytes, such as micro-ribonucleic acids (microRNAs or miRNAs). The invention may therefore relate to a multiplex assay for determining the presence or absence of each target analyte in a group of multiple analytess. The invention uses one or more probes comprising a quadruplex and transmembrane pores.Type: GrantFiled: November 6, 2013Date of Patent: June 12, 2018Assignee: Oxford Nanopore Technologies Ltd.Inventors: Daniel George Fordham, Nicholas Smith, Daniel John Turner
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Publication number: 20180148481Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: ApplicationFiled: September 1, 2015Publication date: May 31, 2018Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit BrusselInventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Publication number: 20180143178Abstract: Arrangements are disclosed for measuring small electrical currents with high sensitivity, for example in the context of sensing molecular entities, for example via interactions between the molecular entities and a membrane protein inserted in an amphiphilic membrane. In one arrangement there is provided a current sensing circuit (52) configured to integrate the current output by a sensor element (56) during each of a plurality of sensing frames (62). In each sensing frame (62) first and second analogue samples of the integral are taken during first and second time windows (71,72). A readout circuit (54) processes the first and second analogue samples to output a digital output signal representing the current output by the sensor element (56). The processing comprises analogue to digital conversion processing and output processing. The output processing is performed exclusively during periods outside of the first and second time windows.Type: ApplicationFiled: May 9, 2016Publication date: May 24, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: David A. Fish, Steven Paul White
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Patent number: 9957560Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.Type: GrantFiled: July 25, 2012Date of Patent: May 1, 2018Assignee: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
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Publication number: 20180095066Abstract: The invention relates to a novel method of producing hetero-oligomeric pores. The invention also relates to hetero-oligomeric pores produced using the method and polynucleotide characterisation using the hetero-oligomeric pores.Type: ApplicationFiled: February 17, 2016Publication date: April 5, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Joseph Kilgour, Neil Roger Wood
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Publication number: 20180087101Abstract: The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled from the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.Type: ApplicationFiled: March 31, 2015Publication date: March 29, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: James Anthony Clarke, Marion Louise Crawford, James White
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Patent number: 9927398Abstract: To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.Type: GrantFiled: June 30, 2015Date of Patent: March 27, 2018Assignee: Oxford Nanopore Technologies Ltd.Inventors: Stuart William Reid, Terence Alan Reid, James Anthony Clarke, Steven Paul White, Gurdial Singh Sanghera
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Publication number: 20180037874Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: ApplicationFiled: September 10, 2014Publication date: February 8, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Mark Bruce, Andrew John Heron, Ruth Moysey, Szabolcs Soeroes, Elizabeth Jayne Wallace, James White
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Publication number: 20180030530Abstract: The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.Type: ApplicationFiled: August 11, 2017Publication date: February 1, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Ruth Moysey, Andrew John Heron
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Publication number: 20180030526Abstract: The invention relates to hetero-oligomeric pores derived from Msp. The invention also relates to polynucleotide characterisation using the hetero-oligomeric pores.Type: ApplicationFiled: February 17, 2016Publication date: February 1, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, James Anthony Clarke, Richard George Hambley, Andrew John Heron, Lakmal Jayasinghe, John Milton, Jonathan Bankes Pugh, Elizabeth Jayne Wallace
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Publication number: 20170363577Abstract: To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.Type: ApplicationFiled: February 16, 2017Publication date: December 21, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Stuart William Reid, Terence Alan Reid, James Anthony Clarke, Steven Paul White, Gurdial Singh Sanghera
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Publication number: 20170356037Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp.Type: ApplicationFiled: May 1, 2015Publication date: December 14, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Publication number: 20170350859Abstract: An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.Type: ApplicationFiled: April 19, 2017Publication date: December 7, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, James Peter Willcocks
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Publication number: 20170335384Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: August 3, 2017Publication date: November 23, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
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Publication number: 20170327880Abstract: An analysis apparatus for performing biochemical analysis of a sample using nanopores comprises: a sensor device that that supports plural nanopores, reservoirs holding material for performing the analysis; a fluidics system; and plural containers for receiving respective samples, all arranged in a cartridge that is removably attachable to an electronics unit arranged to generate drive signals to perform signal processing circuit to generate output data representing the results of the analysis. The fluidics system supplies samples selectively from the containers to the sensor device using a rotary valve. In one valve, a stator defines a plurality of first ports arranged around the rotational axis and a collection chamber extending in around the axis of rotation in communication with a second port. A rotor provides a passage extending between the collection chamber and a position in communication with any one of the plurality of first ports.Type: ApplicationFiled: January 30, 2017Publication date: November 16, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventor: Anthony Jones
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Publication number: 20170326550Abstract: A detachable electrical device can be formed from a kit comprising a pair of component parts adapted for connection to each other, wherein the connected components of the device may be subsequently disconnected, comprising: an array of electrical connectors, each electrical connector comprising an electrically conductive liquid; and an array of electrodes; wherein the arrays can be brought into contact with each other so as to provide a plurality of electrical connections between the electrically conductive liquid of the array of electrical connectors and the electrodes of the array of electrodes, and wherein the electrical connections may be subsequently broken by detaching the electrically conductive liquid from the electrodes of the array.Type: ApplicationFiled: October 15, 2015Publication date: November 16, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, Jason Robert Hyde, Mark David Jackson, Paul Raymond Mackett, Jonathan Edward Mckendry, Richard Kenneth John Wiltshire