Abstract: The present invention provides a new procedure for the synthesis of cDNA from single cells after microdissection. It has the advantage that it is cost-efficient and can be carried out quickly with only few steps, even by less skilled laboratory employees. For the first time, the time-consuming and risky step of RNA isolation is omitted during cDNA synthesis from single cells by performing lysis and cDNA synthesis in the same reaction tube and in one buffer solution, which provides reliable and contamination-free results. The buffer is composed of NP40, carrier-RNA and Super RNAsin, as well as dNTPs and cDNA synthesis primers.

Abstract: The present invention provides a new procedure for the synthesis of CDNA from single cells after microdissection. It has the advantage that it is cost-efficient and can be carried out quickly with only few steps, even by less skilled laboratory employees. For the first time, the time-consuming and risky step of RNA isolation is omitted during cDNA synthesis from single cells by performing lysis and cDNA synthesis in the same reaction tube and in one buffer solution, which provides reliable and contamination-free results. The buffer is composed of NP40, carrier-RNA and Super RNAsin, as well as dNTPs and cDNA synthesis primers.

Abstract: The subject matter of the invention is the use of a device for the application of a fluid which features ozone or ozonized oxygen into a human or animal body and a device for this. The creation of a use and device which use the beneficial effects of ozone to combat tumors without causing side effects which are damaging to someone's health is achieved by applying the fluid intraperitoneally.