Patents Assigned to PhyNexus, Inc.
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Publication number: 20230408383Abstract: The present invention relates to the readily automated methods for the separation of biomolecules, such as proteins or nucleic acids. More specifically, the invention relates to a separation device, which includes at least one column provided with a bottom liquid inlet/outlet and adapted to receive a separation media. At least part of an elongated frit has been arranged substantially parallel to the column side walls, segregating the separation media from the bottom liquid inlet/outlet. The elongated frit may be shaped as a tube and should have a porosity that serves to retain separation media within the column during sample processing. Further, the invention relates to a method of separating at least one target molecule from a liquid, which method comprises providing at least one column provided with a bottom liquid inlet/outlet and optionally with a top liquid inlet.Type: ApplicationFiled: October 6, 2021Publication date: December 21, 2023Applicant: PHYNEXUS, INC.Inventors: Gihoon GO, Don B. APLAON, Fanny HUANG, Douglas T. GJERDE
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Patent number: 11274292Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: GrantFiled: May 2, 2017Date of Patent: March 15, 2022Assignee: PHYNEXUS, INC.Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Publication number: 20210087551Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: ApplicationFiled: December 7, 2020Publication date: March 25, 2021Applicant: PHYNEXUS, INC.Inventors: Chris SUH, Carrie Loan Kim HUYNH, Lee HOANG, Douglas T. GJERDE, Jonathan Michael GRAMBOW
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Patent number: 10883100Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: GrantFiled: April 9, 2017Date of Patent: January 5, 2021Assignee: PhyNexus, IncInventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Patent number: 10597652Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: GrantFiled: October 11, 2016Date of Patent: March 24, 2020Assignee: PHYNEXUS, INC.Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Patent number: 9328342Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.Type: GrantFiled: August 25, 2014Date of Patent: May 3, 2016Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde, Carrie Loan Kim Huynh
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Patent number: 9114383Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods of storing columns in a wet state.Type: GrantFiled: August 17, 2004Date of Patent: August 25, 2015Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Ronald Jones, Allen Burge, Christopher P. Hanna, Leon S. Yengoyan
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Patent number: 8921539Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.Type: GrantFiled: March 29, 2012Date of Patent: December 30, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde
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Patent number: 8816064Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.Type: GrantFiled: September 30, 2011Date of Patent: August 26, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde
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Patent number: 8748194Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.Type: GrantFiled: August 1, 2013Date of Patent: June 10, 2014Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher P. Hanna
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Patent number: 8679857Abstract: The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.Type: GrantFiled: January 28, 2013Date of Patent: March 25, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Jennifer E. Grant, Douglas T. Gjerde
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Patent number: 8664378Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.Type: GrantFiled: September 30, 2011Date of Patent: March 4, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde
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Patent number: 8501116Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.Type: GrantFiled: August 9, 2011Date of Patent: August 6, 2013Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher P. Hanna
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Patent number: 8377715Abstract: The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.Type: GrantFiled: April 26, 2010Date of Patent: February 19, 2013Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Jennifer E. Grant, Douglas T. Gjerde
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Patent number: 8362219Abstract: The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. In some embodiments, the analyte is a protein. The invention further provides reagents, channels, columns and instrumentation related to this and other methods.Type: GrantFiled: January 11, 2010Date of Patent: January 29, 2013Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher P. Hanna
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Patent number: 8361806Abstract: The invention provides, inter alia, methods, devices and reagents for the preparation of native and non-denatured biomolecules using solid-phase extraction channels. The invention is particularly suited for the purification, concentration and/or analysis of protein analytes. The invention further provides, inter alia, methods, devices and reagents for the purification, concentration and/or analysis of multi-protein complexes.Type: GrantFiled: January 6, 2011Date of Patent: January 29, 2013Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher T. Hanna
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Patent number: 8148168Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.Type: GrantFiled: July 15, 2010Date of Patent: April 3, 2012Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Allen Burge, Ronald Jones, Mark Abel
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Patent number: 8148169Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the method involves adjusting the head pressure of the column during the process, or otherwise controlling or regulating the head pressure.Type: GrantFiled: April 21, 2011Date of Patent: April 3, 2012Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Ronald Jones, Allen Burge
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Patent number: 8143071Abstract: The invention provides columns and methods for the purification and concentration of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution. The columns typically include a bed of extraction medium positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. The invention also provides methods for purifying and concentrating multiple analytes simultaneously.Type: GrantFiled: December 21, 2007Date of Patent: March 27, 2012Assignee: PhyNexus, Inc.Inventor: Douglas T. Gjerde
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Patent number: 8057668Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides columns characterized by low backpressure.Type: GrantFiled: October 28, 2010Date of Patent: November 15, 2011Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher P. Hanna