Patents Assigned to PhyNexus, Inc.
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Patent number: 8053247Abstract: The invention provides methods and apparatus for automated sample preparation for MALDI-TOF MS using columns in combination with a liquid handling system. The samples are typically biological such as serum or urine. The columns typically include a bed of media positioned within a modified pipette tip. In some embodiments, the invention provides methods for storing the prepared samples.Type: GrantFiled: October 10, 2007Date of Patent: November 8, 2011Assignee: PhyNexus, Inc.Inventors: Isabel Feuerstein, Günther Bonn, Douglas T. Gjerde, Christian Huck, Guenther Stecher
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Patent number: 7943393Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the method involves adjusting the head pressure of the column during the process, or otherwise controlling or regulating the head pressure.Type: GrantFiled: July 20, 2004Date of Patent: May 17, 2011Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Ronald Jones, Allen Burge
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Patent number: 7879621Abstract: The invention provides, inter alia, methods, devices and reagents for the preparation of native and non-denatured biomolecules using solid-phase extraction channels. The invention is particularly suited for the purification, concentration and/or analysis of protein analytes. The invention further provides, inter alia, methods, devices and reagents for the purification, concentration and/or analysis of multi-protein complexes.Type: GrantFiled: December 10, 2003Date of Patent: February 1, 2011Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher T. Hanna
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Patent number: 7875462Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.Type: GrantFiled: March 23, 2010Date of Patent: January 25, 2011Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Allen Burge, Ronald Jones, Mark Abel
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Patent number: 7837871Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides columns characterized by low backpressure.Type: GrantFiled: August 17, 2004Date of Patent: November 23, 2010Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher P. Hanna
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Patent number: 7722820Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.Type: GrantFiled: November 21, 2005Date of Patent: May 25, 2010Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Allen Burge, Ronald Jones, Mark Abel
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Patent number: 7667010Abstract: The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. The invention further provides reagents, columns and instrumentation related to this and other methods.Type: GrantFiled: August 22, 2006Date of Patent: February 23, 2010Assignee: PhyNexus, Inc.Inventors: Douglas T. Gierde, Christopher T. Hanna
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Patent number: 7612165Abstract: The invention provides, inter alia, methods for synthesizing a molecule on the channel surface of a capillary, comprising the steps of: (i) covalently attaching a first chemical entity to the channel surface of a capillary; and (ii) covalently attaching a second chemical entity to the first chemical entity, wherein the covalent attachment steps are part of a process for synthesizing a molecule on the channel surface.Type: GrantFiled: February 24, 2006Date of Patent: November 3, 2009Assignee: PhyNexus, Inc.Inventor: Douglas T. Gjerde
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Patent number: 7488603Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the elution of analyte in a small volume of liquid.Type: GrantFiled: August 17, 2004Date of Patent: February 10, 2009Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher P. Hanna
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Patent number: 7482169Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.Type: GrantFiled: July 14, 2003Date of Patent: January 27, 2009Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher T. Hanna
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Patent number: 7151167Abstract: The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; and eluting the analyte by passing a desorption solution through the channel, wherein the method includes a step wherein a multiple-pass solution is passed through at least some substantial portion of the extraction channel at least twice. Non-limiting examples multiple-pass solutions include a solution containing an analyte, a wash solution and/or a desorption solution.Type: GrantFiled: June 9, 2004Date of Patent: December 19, 2006Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher P. Hanna
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Patent number: 7122640Abstract: The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. The invention further provides reagents, columns and instrumentation related to this and other methods.Type: GrantFiled: March 4, 2004Date of Patent: October 17, 2006Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Christopher T. Hanna