Patents Assigned to PULSE BIOSCIENCES, INC.
  • Publication number: 20190239949
    Abstract: Techniques for treating a tumor and vaccinating against cancer are described. The techniques include treating the tumor by positioning electrodes over an interface between the tumor and non-tumor tissue and applying sub-microsecond pulsed electric fields. The positioning is facilitated by an imaginary contour line of a threshold value of the electric field. In an example, the imaginary contour line is overlaid over images that include the tumor such that the electrodes are properly positioned over the tumor. The techniques also include vaccinating against cancer by passing sub-microsecond pulsed electric fields through tumor cells of a subject sufficient to cause the tumor cells to express calreticulin on surface membranes. The tumor cells are extracted and introduced with the expressed calreticulin into the subject or another subject, thereby providing a vaccination.
    Type: Application
    Filed: April 15, 2019
    Publication date: August 8, 2019
    Applicant: Pulse Biosciences, Inc.
    Inventors: Richard Lee Nuccitelli, Jon Casey Berridge, Zachary Mallon, Mark Kreis, Brian Athos, Pamela Nuccitelli
  • Patent number: 10307207
    Abstract: Techniques for treating a tumor and vaccinating against cancer are described. The techniques include treating the tumor by positioning electrodes over an interface between the tumor and non-tumor tissue and applying sub-microsecond pulsed electric fields. The positioning is facilitated by an imaginary contour line of a threshold value of the electric field. In an example, the imaginary contour line is overlaid over images that include the tumor such that the electrodes are properly positioned over the tumor. The techniques also include vaccinating against cancer by passing sub-microsecond pulsed electric fields through tumor cells of a subject sufficient to cause the tumor cells to express calreticulin on surface membranes. The tumor cells are extracted and introduced with the expressed calreticulin into the subject or another subject, thereby providing a vaccination.
    Type: Grant
    Filed: July 19, 2018
    Date of Patent: June 4, 2019
    Assignee: PULSE BIOSCIENCES, INC.
    Inventors: Richard Lee Nuccitelli, Jon Casey Berridge, Zachary Mallon, Mark Kreis, Brian Athos, Pamela Nuccitelli
  • Publication number: 20190160283
    Abstract: In one aspect, methods of treating human papillomavirus (HPV)-associated growths are provided in which nano-pulse stimulation is applied at the site of a cancer. In another aspect, devices and computer systems for delivering nano-pulse stimulation for the treatment of HPV-associated growths are provided.
    Type: Application
    Filed: November 28, 2017
    Publication date: May 30, 2019
    Applicant: Pulse Biosciences, Inc.
    Inventors: Richard Lee Nuccitelli, Darrin Robert Uecker
  • Patent number: 10252050
    Abstract: The pulse applicator includes a first arm, including a first electrode, a second arm, including a second electrode, and a spacer. The first arm, the spacer, and the second arm are movably connected, and define a gap between the first arm and the second arm. The first electrode, the gap, and the second electrode are selectively alignable, and the first electrode and the second electrode are configured to deliver an electrical field across the gap in response to an electrical pulse received across the first and second electrodes.
    Type: Grant
    Filed: May 15, 2017
    Date of Patent: April 9, 2019
    Assignee: PULSE BIOSCIENCES, INC.
    Inventors: Mark P. Kreis, David J. Danitz, Cameron D. Hinman
  • Publication number: 20190021811
    Abstract: This disclosure relates to an in vivo treatment of tissue, for example, a skin lesion of a mammal comprising application of electrical energy to the skin lesion in a form of electrical pulses. At least one electrical pulse is applied. The pulse duration may be at least 1 nanosecond. Surface of a tissue surrounding the skin lesion may be marked to guide the device to deliver the electric pulses at substantially precise locations on the lesion surface. This treatment may prevent at least growth of the lesion.
    Type: Application
    Filed: September 21, 2018
    Publication date: January 24, 2019
    Applicant: Pulse Biosciences, Inc.
    Inventors: Cesar Escobar Blanco, Elena Tovkan Forster, Stefani Reiko Takahashi
  • Publication number: 20190022141
    Abstract: Nanosecond pulsed electric field (nsPEF) treatments of a tumor are adjusted based on a size and type of the tumor to stimulate an immune response against the tumor and other tumors in the subject. Calreticulin expression on tumor cells can be detected to confirm treatment. An immune response biomarker can be measured, and further nsPEF treatments can be performed if needed to stimulate or further stimulate the immune response. Cancers that have metastasized may be treated by directly treating a tumor that is most accessible. The treatment can be combined with CD47-blocking antibodies, doxorubicin, CTLA-4-blocking antibodies, and/or PD-1-blocking antibodies. Electrical characteristics of nsPEF treatments can be based on the size, type, and/or strength of tumors and/or a quantity of tumors in the subject.
    Type: Application
    Filed: September 26, 2018
    Publication date: January 24, 2019
    Applicant: Pulse Biosciences, Inc.
    Inventors: Richard Lee Nuccitelli, Pamela S. Nuccitelli, Joanne Lum, Kaying Lui, Brian G. Athos, Mark P. Kreis, Zachary R. Mallon, Jon Berridge
  • Patent number: 10137152
    Abstract: A subject is inoculated from a disease by exposing a biopsy of a tumor or other abnormal growth to a nanosecond pulsed electric field (nsPEF). A sufficient treatment can be confirmed by detecting calreticulin on the tumor cell membranes, which indicates apoptosis occurring in the tumor cells. Treated tumor cells from the biopsy are then reintroduced into the subject. The calreticulin-exhibiting tumor cells activate the subject's immune system against the tumor, and any other like tumors in the body, and effectively vaccinates the subject against the disease. The treatment can be combined with CD47-blocking antibodies, doxorubicin, CTLA-4-blocking antibodies, and/or PD-1-blocking antibodies. The immune response may be measured at a later time. Specific electrical characteristics of the nsPEF treatments can be based on the type and/or strength of the tumor.
    Type: Grant
    Filed: January 17, 2018
    Date of Patent: November 27, 2018
    Assignee: Pulse Biosciences, Inc.
    Inventors: Richard Lee Nuccitelli, Pamela S. Nuccitelli, Joanne Lum, Kaying Lui, Brian G. Athos, Mark P. Kreis, Zachary R. Mallon, Jon Berridge
  • Publication number: 20180318004
    Abstract: Techniques for treating a tumor and vaccinating against cancer are described. The techniques include treating the tumor by positioning electrodes over an interface between the tumor and non-tumor tissue and applying sub-microsecond pulsed electric fields. The positioning is facilitated by an imaginary contour line of a threshold value of the electric field. In an example, the imaginary contour line is overlaid over images that include the tumor such that the electrodes are properly positioned over the tumor. The techniques also include vaccinating against cancer by passing sub-microsecond pulsed electric fields through tumor cells of a subject sufficient to cause the tumor cells to express calreticulin on surface membranes. The tumor cells are extracted and introduced with the expressed calreticulin into the subject or another subject, thereby providing a vaccination.
    Type: Application
    Filed: July 19, 2018
    Publication date: November 8, 2018
    Applicant: Pulse Biosciences, Inc.
    Inventors: Richard Lee Nuccitelli, Jon Casey Berridge, Zachary Mallon, Mark Kreis, Brian Athos, Pamela Nuccitelli
  • Patent number: 10105191
    Abstract: This disclosure relates to an in vivo treatment of a skin lesion of a mammal comprising application of electrical energy to the skin lesion in a form of electrical pulses. At least one electrical pulse is applied. The pulse duration may be at least 1 nanosecond at the full-width-half-maximum. Surface of a tissue surrounding the skin lesion may be marked to guide the device to deliver the electric pulses at substantially precise locations on the lesion surface. This treatment may prevent at least growth of the lesion.
    Type: Grant
    Filed: August 31, 2016
    Date of Patent: October 23, 2018
    Assignee: PULSE BIOSCIENCES, INC.
    Inventors: Cesar Escobar Blanco, Elena Tovkan Forster, Stefani Reiko Takahashi
  • Publication number: 20180243558
    Abstract: A pulse generation system is disclosed. The pulse generation system includes a controller, an output terminal, and a plurality of pulse generator circuits. The controller is configured to cause a driving signal pulse to be transmitted to any selected one or more of the pulse generator circuits, and to cause the driving signal pulse to not be transmitted to any selected one or more other pulse generator circuits. Each of the pulse generator circuits is configured to generate an output voltage pulse at the output terminal in response to the driving signal pulse being transmitted thereto.
    Type: Application
    Filed: February 28, 2017
    Publication date: August 30, 2018
    Applicant: Pulse Biosciences, Inc.
    Inventors: Brian G. Athos, Shu Xiao, David J. Danitz, Mark P. Kreis, Darrin R. Uecker
  • Patent number: 10058383
    Abstract: Techniques for treating a tumor and vaccinating against cancer are described. The techniques include treating the tumor by positioning electrodes over an interface between the tumor and non-tumor tissue and applying sub-microsecond pulsed electric fields. The positioning is facilitated by an imaginary contour line of a threshold value of the electric field. In an example, the imaginary contour line is overlaid over images that include the tumor such that the electrodes are properly positioned over the tumor. The techniques also include vaccinating against cancer by passing sub-microsecond pulsed electric fields through tumor cells of a subject sufficient to cause the tumor cells to express calreticulin on surface membranes. The tumor cells are extracted and introduced with the expressed calreticulin into the subject or another subject, thereby providing a vaccination.
    Type: Grant
    Filed: August 7, 2017
    Date of Patent: August 28, 2018
    Assignee: PULSE BIOSCIENCES, INC.
    Inventors: Richard Lee Nuccitelli, Jon Casey Berridge, Zachary Mallon, Mark Kreis, Brian Athos, Pamela Nuccitelli
  • Publication number: 20180153937
    Abstract: A subject is inoculated from a disease by exposing a biopsy of a tumor or other abnormal growth to a nanosecond pulsed electric field (nsPEF). A sufficient treatment can be confirmed by detecting calreticulin on the tumor cell membranes, which indicates apoptosis occurring in the tumor cells. Treated tumor cells from the biopsy are then reintroduced into the subject. The calreticulin-exhibiting tumor cells activate the subject's immune system against the tumor, and any other like tumors in the body, and effectively vaccinates the subject against the disease. The treatment can be combined with CD47-blocking antibodies, doxorubicin, CTLA-4-blocking antibodies, and/or PD-1-blocking antibodies. The immune response may be measured at a later time. Specific electrical characteristics of the nsPEF treatments can be based on the type and/or strength of the tumor.
    Type: Application
    Filed: January 17, 2018
    Publication date: June 7, 2018
    Applicant: Pulse Biosciences, Inc.
    Inventors: Richard Lee Nuccitelli, Pamela S. Nuccitelli, Joanne Lum, Kaying Lui, Brian G. Athos, Mark P. Kreis, Zachary R. Mallon, Jon Berridge
  • Publication number: 20180147403
    Abstract: This disclosure relates to an in vivo treatment of a skin lesion of a mammal comprising application of electrical energy to the skin lesion in a form of electrical pulses. At least one electrical pulse is applied. The pulse duration may be at least 1 nanosecond at the full-width-half-maximum. This treatment may prevent at least growth of the lesion.
    Type: Application
    Filed: January 26, 2018
    Publication date: May 31, 2018
    Applicant: Pulse Biosciences, Inc.
    Inventors: Jack Robert Weissberg, Sudeep Deshpande, Chunqi Jiang
  • Patent number: 9956391
    Abstract: This disclosure relates to an in vivo treatment of a skin lesion of a mammal comprising application of electrical energy to the skin lesion in a form of electrical pulses. At least one electrical pulse is applied. The pulse duration may be at least 1 nanosecond at the full-width-half-maximum. This treatment may prevent at least growth of the lesion.
    Type: Grant
    Filed: December 10, 2012
    Date of Patent: May 1, 2018
    Assignee: Pulse Biosciences, Inc.
    Inventors: Jack Robert Weissberg, Sudeep Deshpande, Chunqi Jiang
  • Publication number: 20180078755
    Abstract: An electrode connectable to a pulse generator is disclosed. The electrode includes therapeutic terminals configured to deliver the pulse to a patient, first and second electrical pulse inlet holes, and a first pulse input terminal. The first pulse input terminal is in the first electrical pulse inlet hole and is spaced apart from an entrance to the first electrical pulse inlet hole by more than about 2.5 cm. Also, first pulse input terminal is connected with one or more of the therapeutic terminals. The electrode also includes a second pulse input terminal, where the second pulse input terminal is in the second electrical pulse inlet hole and is spaced apart from an entrance to the second electrical pulse inlet hole by a distance greater than about 2.5 cm. Also, the second pulse input terminal is electrically connected with one or more of the therapeutic terminals.
    Type: Application
    Filed: September 19, 2016
    Publication date: March 22, 2018
    Applicant: PULSE BIOSCIENCES, INC.
    Inventors: Mark P. Kreis, David J. Danitz, Cameron Dale Hinman, Sean Nicholas Finson
  • Publication number: 20180001079
    Abstract: This disclosure relates to an in vivo treatment of a skin lesion of a mammal comprising application of electrical energy to the skin lesion in a form of electrical pulses. At least one electrical pulse is applied. The pulse duration may be at least 0.01 nanoseconds at the full-width-at-half-maximum. This treatment may at least prevent growth of the lesion.
    Type: Application
    Filed: August 11, 2017
    Publication date: January 4, 2018
    Applicant: Pulse Biosciences, Inc.
    Inventors: Jack Robert Weissberg, Gary Steven Lazar, Dong Yin
  • Publication number: 20170360504
    Abstract: Techniques for treating a tumor and vaccinating against cancer are described. The techniques include treating the tumor by positioning electrodes over an interface between the tumor and non-tumor tissue and applying sub-microsecond pulsed electric fields. The positioning is facilitated by an imaginary contour line of a threshold value of the electric field. In an example, the imaginary contour line is overlaid over images that include the tumor such that the electrodes are properly positioned over the tumor. The techniques also include vaccinating against cancer by passing sub-microsecond pulsed electric fields through tumor cells of a subject sufficient to cause the tumor cells to express calreticulin on surface membranes. The tumor cells are extracted and introduced with the expressed calreticulin into the subject or another subject, thereby providing a vaccination.
    Type: Application
    Filed: August 7, 2017
    Publication date: December 21, 2017
    Applicant: Pulse Biosciences, Inc.
    Inventors: Richard Lee Nuccitelli, Jon Casey Berridge, Zachary Mallon, Mark Kreis, Brian Athos, Pamela Nuccitelli
  • Publication number: 20170333112
    Abstract: An optimization of electrical characteristics for treatments of tumor or other abnormal cells in culture with sub-microsecond, high-electric field electrical pulses is disclosed. The voltages, pulse widths, and number of pulses are chosen such that the treatment energy is 10-20 J/mL. That is, U=n*?t*V*I/volume is 10-20 J/mL, in which n is the number of pulses, ?t is the duration of each pulse, V is the voltage, I is current, and volume is the area of parallel electrodes times the distance between them. V divided by the distance between the electrodes can be in an effective range of 6 kV/cm to 30 kV/cm, 60 kV/cm, 100 kV/cm, or higher intensities. Rows of needle electrodes, blade electrodes, or other configurations of electrodes can approximate parallel electrodes.
    Type: Application
    Filed: May 19, 2017
    Publication date: November 23, 2017
    Applicant: Pulse Biosciences, Inc.
    Inventors: Richard L. Nuccitelli, Zachary R. Mallon, Amanda H. McDaniel, David J. Danitz, Brian G. Athos, Mark P. Kreis, Darrin R. Uecker, Pamela S. Nuccitelli
  • Publication number: 20170326361
    Abstract: The pulse applicator includes a first arm, including a first electrode, a second arm, including a second electrode, and a spacer. The first arm, the spacer, and the second arm are movably connected, and define a gap between the first arm and the second arm. The first electrode, the gap, and the second electrode are selectively alignable, and the first electrode and the second electrode are configured to deliver an electrical field across the gap in response to an electrical pulse received across the first and second electrodes.
    Type: Application
    Filed: May 15, 2017
    Publication date: November 16, 2017
    Applicant: Pulse Biosciences, Inc.
    Inventors: Mark P. Kreis, David J. Danitz, Cameron D. Hinman
  • Publication number: 20170319851
    Abstract: A method of testing a therapeutic pulse generator circuit is disclosed. The method includes charging the pulse generator circuit to a first charge voltage, with the pulse generator circuit, delivering a first voltage pulse to a load through an electrode, and determining an impedance of the load with the first voltage pulse. The method also includes comparing the impedance with an expected impedance, as a result of the comparison, determining to deliver a second voltage pulse to the load based, and delivering the second voltage pulse to the load, where at least one of the first and second voltage pulses is therapeutic to the load.
    Type: Application
    Filed: November 9, 2016
    Publication date: November 9, 2017
    Applicant: Pulse Biosciences, Inc.
    Inventors: Brian G. Athos, David J. Danitz, Mark P. Kreis, Darrin R. Uecker