Patents Assigned to QIAGEN GmbH
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Patent number: 11359237Abstract: Modular flow cells, devices with modular flow cells, and methods of sequencing using modular flow cells, as well as systems and kits including modular flow cells, are described, permitting sequencing wherein less than the full capacity for sequencing is desired.Type: GrantFiled: April 23, 2020Date of Patent: June 14, 2022Assignees: QIAGEN SCIENCES, LLC, QIAGEN GmbHInventors: Jerzy Olejnik, Dirk Zimmermann
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Patent number: 11345952Abstract: PCR that allows the researchers to amplify a desired DNA requiring only tiny amounts of sample. Such amplification reactions are technically challenging and are often hampered by several practical issues such as the presence of PCR inhibitors, sample degradation and low quantities of said sample. The invention addresses these issues with a method for evaluating the amplification efficiency and/or the presence of inhibitors and/or degradation and/or performing a quantification of a nucleic acid in a real-time amplification reaction comprising: optionally amplifying in a reaction composition a first target nucleic acid using a first primer pair in a real-time amplification reaction, (i) amplifying in said reaction composition one or more second internal nucleic acid control templates (IC) with a length of between 50 and 2000 nucleotides, wherein the second nucleic acid has a sequence selected from the group of: i) SEQ ID NO.Type: GrantFiled: September 15, 2017Date of Patent: May 31, 2022Assignee: Qiagen GmbHInventors: Miroslav Vranes, Ralf Peist, Mario Scherer, Stefan Otto Cornelius
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Patent number: 11253797Abstract: The present invention relates to a chromatographic device for isolating and purifying nucleic acids, preferably genomic DNA, by gel filtration chromatography, a method for isolating and purifying nucleic acids, preferably genomic DNA, using this device and a kit comprising this device.Type: GrantFiled: July 7, 2016Date of Patent: February 22, 2022Assignee: QIAGEN GmbHInventors: Roland Fabis, Markus Müller, Jörg Hucklenbroich, Mario Scherer
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Patent number: 11248262Abstract: The invention refers to a novel method of preparing strand-specific RNA-sequencing libraries that can be used to identify DNA coding and non-coding strands that are transcribed to RNA. Such strand-specific RNA-sequencing libraries are especially useful in discovering anti-sense RNA and non-coding RNA. Random primer oligonucleotides, covalently coupled to a moiety, which blocks ligation, are used for RT reaction or the subsequent generation of the second DNA strand so that only one strand of the generated double-stranded DNA is ligated to sequencing adapters at the 5? nucleotide and sequenced by paired-end sequencing.Type: GrantFiled: August 24, 2016Date of Patent: February 15, 2022Assignee: QIAGEN GmbHInventors: Nan Fang, Bernhard Noll, Katja Heitz
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Patent number: 11220705Abstract: The present invention is directed to a method for immobilizing nucleic molecule on solid support and to a use of a nucleic acid non-immobilized primer in combination with a nucleic acid primer linked to a solid support in said a method.Type: GrantFiled: April 25, 2016Date of Patent: January 11, 2022Assignee: QIAGEN GmbHInventors: Christian Korfhage, Evelyn Fricke
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Patent number: 11203777Abstract: The present invention provides a method for preparing a sterilized composition suitable for stabilizing an extracellular nucleic acid population of a biological sample. Also provided are sterilizable compositions, wherein the compositions in sterilized form are suitable for stabilizing an extracellular nucleic acid population of a biological sample. Further useful methods, devices, kits and uses are also provided. Further sterilisable and sterilized compositions described herein are also suitable to stabilize intracellular nucleic acids (e.g. intracellular DNA such as genomic DNA and/or intracellular RNA) and cell characteristics, such as e.g. cell surface proteins and/or the cell morphology.Type: GrantFiled: November 21, 2016Date of Patent: December 21, 2021Assignee: QIAGEN GmbHInventors: Daniel Grölz, Andrea Ullius
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Publication number: 20210390666Abstract: Method and system for compensating intensity biases in a plurality of digital images. Each digital image of the plurality of digital images contains a plurality of objects and each of the plurality of objects is configured to receive at least one molecule comprising genetic information, wherein the at least one molecule is configured to receive one of at least a first fluorescent compound and a second fluorescent compound. A first digital image of the plurality of digital images is taken by an optical imaging system during emission of electromagnetic radiation by the first fluorescent compound, and a second digital image of the plurality of digital images is taken by the optical imaging system during emission of electromagnetic radiation by the second fluorescent compound.Type: ApplicationFiled: March 29, 2021Publication date: December 16, 2021Applicant: QIAGEN GmbHInventors: Thorsten ZERFASS, Maiko LOHEL, Fernando Carrillo OESTERREICH
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Publication number: 20210374915Abstract: The invention relates to a method of neighbor influence compensation between a plurality of objects in at least one digital image, wherein the at least one digital image contains image information about a plurality of objects. Each of the plurality of objects is configured to receive at least one molecule comprising genetic information, wherein the at least one molecule is configured to receive a fluorescent compound, and the at least one digital image is taken by an optical imaging system during emission of electromagnetic radiation of the fluorescent compounds received by the at least one molecules.Type: ApplicationFiled: September 15, 2017Publication date: December 2, 2021Applicant: QIAGEN GmbHInventors: Maiko LOHEL, Thorsten ZERFASS
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Patent number: 11149304Abstract: The present invention relates to a method for assessing the status of nucleic acid degradation and/or integrity of one or more nucleic acids in a sample, comprising the steps amplifying at least two overlapping regions within at least one locus (e.g. by heminested or nested PCR), and detecting the amount of the at least two amplification products through the use of at least two probes, wherein one probe binds to the region of overlap and the at least one other probe binds to a non-overlapping region. The invention further relates to a method of designing primers and/or probes for amplifying at least two overlapping regions within at least one locus, wherein the locus that is amplified is a single copy locus (SCL) or multicopy locus (MLC). The invention also relates to a primer and a primer pair for amplifying at least two overlapping regions from one nucleic acid template which is a multicopy locus present in 21 loci in the human genome. These primers and probes may be in a kit.Type: GrantFiled: September 15, 2017Date of Patent: October 19, 2021Assignee: Qiagen GmbHInventors: Miroslav Vranes, Ralf Peist, Mario Scherer, Stefan Otto Cornelius
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Patent number: 11111535Abstract: The invention relates to nucleic acid probes, nucleic acid probe libraries, and kits for detecting, classifying, or quantitating components in a complex mixture of nucleic acids, such as a transcriptome, and methods of using the same. The invention also relates to methods of identifying nucleic acid probes useful in the probe libraries and to methods of identifying a means for detection of a given nucleic acid.Type: GrantFiled: September 23, 2016Date of Patent: September 7, 2021Assignee: QIAGEN GmbHInventors: Niels B. Ramsing, Peter Mouritzen, Søren Morgenthaler Echwald, Niels Tolstrup
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Patent number: 11104896Abstract: The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.Type: GrantFiled: June 10, 2016Date of Patent: August 31, 2021Assignee: QIAGEN GmbHInventors: Alexander Wolf, Sandra Hammerschmidt, Thorsten Voss
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Patent number: 11085073Abstract: The present invention is directed to a method for immobilizing a nucleic acid molecule on a solid support and to a use of a combination of a first nucleic acid immobilized primer linked to a solid support and a second immobilized primer linked to said solid support in said method.Type: GrantFiled: April 25, 2016Date of Patent: August 10, 2021Assignee: QIAGEN GmbHInventors: Christian Korfhage, Evelyn Fricke
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Patent number: 11078527Abstract: According to a first aspect of the present invention, a method is provided for detecting and/or quantifying male genomic DNA in a sample, wherein the method comprises the step of amplification of a multicopy locus within the human Y-chromosome (MCL-Y), wherein said locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 3 over a stretch of at least 60 base pairs (bp). A second aspect of the present invention relates to a primer or primer pair which hybridizes under stringent conditions to a sequence according to SEQ ID NO. 3 and/or any of 4 to 11. The invention also relates to a kit.Type: GrantFiled: September 15, 2017Date of Patent: August 3, 2021Assignee: Qiagen GmbHInventors: Miroslav Vranes, Ralf Peist, Mario Scherer, Stefan Otto Cornelius, Margaretha König
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Patent number: 11021736Abstract: The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps: a. binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture; b. separating the solid phase with the bound extracellular nucleic acids; c. optionally washing the extracellular nucleic acids; d. optionally eluting extracellular nucleic acids from the solid phase. The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.Type: GrantFiled: November 29, 2018Date of Patent: June 1, 2021Assignee: Qiagen GmbHInventors: Martin Horlitz, Annette Nocon, Markus Sprenger-Haussels, Peter Grünefeld, Christoph Erbacher
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Patent number: 11021733Abstract: The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample using an apoptosis inhibitor, preferably a caspase inhibitor, a hypertonic agent and/or a compound according to formula 1 as defined in the claims.Type: GrantFiled: November 28, 2018Date of Patent: June 1, 2021Assignee: Qiagen GmbHInventors: Martin Horlitz, Annabelle Schubert, Markus Sprenger-Haussels
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Patent number: 11015192Abstract: The invention relates to a method for preparing a strand-specific library from an nucleic acid or preferably RNA sample, for RNA comprising the steps of: (i) optionally fragmenting said RNA sample, (ii) generating a plurality of first cDNA strands by subjecting said fragmented RNA to reverse transcription by using a reverse transcriptase and first oligonucleotide primers, (iii) generating a plurality of second cDNA strands by using a DNA polymerase, second oligonucleotide primers, and the plurality of first cDNA strands, and (iv) ligating adapters to the 3? and 5? termini of the of double-stranded cDNA, (v) wherein the first cDNA strand allows no adapter ligation at its 5? terminus and said second cDNA strand allows adapter ligation at its 5? terminus, or vice versa, and, (v) optionally cloning, sequencing or otherwise using the strand-specific library.Type: GrantFiled: April 20, 2017Date of Patent: May 25, 2021Assignee: QIAGEN GMBHInventors: Nan Fang, Wolfgang Krebs, Isabell Czolkos
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Patent number: 10991107Abstract: The present invention relates to a method and a system for aligning at least one part of a second digital image with at least one part of a first digital image comprising: dividing the at least one part of the first digital image into a plurality of tiles t_fi; dividing the at least one part of the second digital image into a plurality of tiles t_si; creating a plurality of correlation images fci; determining the position of a global maximum correlation value p_?? for each of the plurality of correlation images fci_??; calculating a plurality of first offset vectors fov, the offset vector fov_?? representing the offset between the tile t_si?? and the corresponding tile t_fi??; and aligning the at least one part of the second digital image with the at least one part of the first digital image by applying the corresponding first offset vectors fov.Type: GrantFiled: December 9, 2016Date of Patent: April 27, 2021Assignee: QIAGEN GmbHInventors: Guido Schuster, Thorsten Zerfass, Thomas Unterer, Jan Hauth, Maiko Lohel
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Patent number: 10963992Abstract: Method and system for compensating intensity biases in a plurality of digital images. Each digital image of the plurality of digital images contains a plurality of objects and each of the plurality of objects is configured to receive at least one molecule comprising genetic information, wherein the at least one molecule is configured to receive one of at least a first fluorescent compound and a second fluorescent compound. A first digital image of the plurality of digital images is taken by an optical imaging system during emission of electromagnetic radiation by the first fluorescent compound, and a second digital image of the plurality of digital images is taken by the optical imaging system during emission of electromagnetic radiation by the second fluorescent compound.Type: GrantFiled: October 20, 2017Date of Patent: March 30, 2021Assignee: QIAGEN GmbHInventors: Thorsten Zerfass, Maiko Lohel, Fernando Carrillo Oesterreich
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Patent number: 10954507Abstract: A phenol-free method for isolating a nucleic acid from a sample is provided, said method comprising the following steps: a) preparing a precipitation mixture by adding at least one metal cation precipitant and at least one organic solvent selected from aprotic polar solvents and protic solvents to the sample, wherein the precipitation mixture i) comprises the metal cation precipitant; ii) comprises the organic solvent in a concentration of 15% or less; iii) comprises a buffering agent; and iv) has an acidic pH value, and precipitating proteins; b) separating the precipitate from the supernatant, wherein the supernatant comprises small RNA having a length of less than 200 nt and large RNA having a length of at least 1000 nt; and c) isolating a nucleic acid from the supernatant. Using an organic solvent as claimed during the protein precipitation step in the defined concentration provides a supernatant which in addition to small RNA also comprises large RNA.Type: GrantFiled: July 17, 2015Date of Patent: March 23, 2021Assignee: Qiagen GmbHInventors: David Löper, Kerstin Steinert
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Publication number: 20210078003Abstract: A flowcell device for a sequencing by synthesis instrument. The flowcell device has a fluid inlet configured to receive one or more liquid reagents, a fluid outlet configured to pass the one or more liquid reagents, and a channel extending between and fluidly connecting the fluid inlet and the fluid outlet. At least a portion of the channel comprises a reflective structure configured to retain a plurality of sequencing targets thereon. The reflective structure includes at least a metal oxide layer and a film having a first surface and a second surface opposed the first surface. The first surface of the film is disposed on the metal oxide layer and the second surface of the film is configured to receive a plurality of sequencing targets immobilized thereon.Type: ApplicationFiled: January 25, 2019Publication date: March 18, 2021Applicants: Qiagen GmbH, Qiagen Sciences, LLCInventors: Sebastian Koeber, Maximilian Focke, Luisa Andruzzi