Patents Assigned to Quest Diagnostics
  • Thyroglobulin quantitation by mass spectrometry
    Patent number: 10309971
    Abstract: Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.
    Type: Grant
    Filed: June 1, 2015
    Date of Patent: June 4, 2019
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Yanni Zhang, Nigel J. Clarke, Richard E. Reitz
  • ANALYSIS OF AMINO ACIDS IN BODY FLUID BY LIQUID CHROMOTOGRAPHY-MASS SPECTROMETRY
    Publication number: 20190145983
    Abstract: This disclosure provides methods for quantifying individual amino acids in various bodily fluids obtained from a human patient. Also provided are reference ranges for normal amino acid levels in the various bodily fluids (e.g., blood plasma, urine, cerebrospinal fluid, and saliva) and for various age groups (e.g., neonates, infants, children, and adults).
    Type: Application
    Filed: January 7, 2019
    Publication date: May 16, 2019
    Applicant: Quest Diagnostics Investments Incorporated
    Inventor: Scott GOLDMAN
  • METHODS FOR CELL-FREE DNA EXTRACTION FOR NON-INVASIVE PRENATAL SCREENING
    Publication number: 20190127728
    Abstract: Provided herein are methods and systems for cell-free DNA extraction from liquid biological samples. The methods can be employed for determination of fetal DNA fraction and non-invasive prenatal screening of fetal aneuploidies and analyses of other types of cell-free DNA.
    Type: Application
    Filed: October 25, 2016
    Publication date: May 2, 2019
    Applicant: Quest Diagnostics Investments Incorporated
    Inventors: Ben ANDERSON, Charles STROM, David TSAO, Yan LIU, Weimin SUN
  • Methods for detecting vitamin D metabolites by mass spectrometry
    Patent number: 10267810
    Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally directed to ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.
    Type: Grant
    Filed: December 20, 2017
    Date of Patent: April 23, 2019
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Nigel J. Clarke, Brett Holmquist, Gloria Kwang-Ja Lee, Richard E. Reitz
  • BCR-ABL TRUNCATION MUTATIONS
    Publication number: 20190112662
    Abstract: Truncation variants of BCR-ABL mRNA that produces BCR-ABL proteins with a truncated C-terminus and its role in resistance to treatment with kinase inhibitors is described. Vectors for expressing the truncated gene products are described as well as recombinant cells that express the truncated gene products from cDNA constructs. Also provided are methods compositions and kits for detecting the BCR-ABL truncation variants. Also provided are methods for determining the prognosis of a patient diagnosed as having myeloproliferative disease, and methods for predicting the likelihood for resistance to a treatment with tyrosine kinase inhibitor in a patient diagnosed as having myeloproliferative disease. Additionally, methods for screening BCR-ABL tyrosine kinase domain inhibitors which rely on the recombinant cells are also disclosed.
    Type: Application
    Filed: October 5, 2018
    Publication date: April 18, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventors: Maher Albitar, Wanlong Ma
  • COMPOSITIONS AND METHODS FOR SCREENING MUTATIONS IN THYROID CANCER
    Publication number: 20190112653
    Abstract: The present technology relates to methods for determining whether a patient having thyroid nodules with indeterminate cytology will benefit from diagnostic surgery, e.g., lobectomy. These methods are based on screening a patient's thyroid nodules and detecting alterations in target nucleic acid sequences corresponding to a specific set of thyroid cancer-related genes. Kits for use in practicing the methods are also provided.
    Type: Application
    Filed: December 30, 2016
    Publication date: April 18, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventors: Shih-Min CHENG, Joseph J. CATANESE, Andrew GRUPE, Feras HANTASH, Frederic M. WALDMAN, Kevin QU
  • UNIVERSAL SANGER SEQUENCING FROM NEXT-GEN SEQUENCING AMPLICONS
    Publication number: 20190112657
    Abstract: Described herein are methods, compositions and kits directed to amplification of nucleic acids suitable for both next generation sequencing (NGS) and a second round of sequencing as validation, such as Sanger sequencing.
    Type: Application
    Filed: October 26, 2018
    Publication date: April 18, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventors: Heather Sanders, Hai-Rong Li, Feras Hantash, Frederic Waldman
  • ASSAYS AND METHODS FOR THE DIAGNOSIS OF POST-STREPTOCOCCAL DISORDERS
    Publication number: 20190107536
    Abstract: Provided are methods for diagnosing a disease in a subject with a previous streptococcal infection by determining the presence or absence of one or more autoantibodies in a biological sample from the subject, wherein the one or more autoantibodies recognize an antigen from a protein selected from the group consisting of ELAVL2, ELAVL3, ELAVL4, Nova-1, Nova-2, Cdr1, Cdr2; and Cdr3. The presence of such autoantibodies is indicative of a positive diagosis for a post-streptococcal disease such as PANDAS, post-GABHS glomerulonephritis, rheumatic fever, autism and Syndenham's chorea.
    Type: Application
    Filed: December 4, 2018
    Publication date: April 11, 2019
    Applicant: I Quest Diagnostics Investments LLC
    Inventors: Stanley J. Naides, Joanna Popov
  • NUCLEIC ACID DETECTION COMBINING AMPLIFICATION WITH FRAGMENTATION
    Publication number: 20190100808
    Abstract: Provided herein are methods and compositions for detection of a nucleic acid target in a sample. The methods and compositions use primer directed amplification in conjunction with nucleic acid fragmentation. The methods have high sensitivity even in the presence of a large amount of non-target nucleic acid. Also provided are oligonucleotides and kits useful in the method. Exemplary nucleic acid targets are those with mutant gene sequence such as mutant sequence of the EGFR, APC, TMPRSS2, ERG and ETV1 genes.
    Type: Application
    Filed: October 19, 2018
    Publication date: April 4, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventors: Heather R. Sanders, Kevin Z. Qu, Charles M. Strom, Richard A. Bender
  • COMPOSITIONS AND METHODS FOR DETECTING MUTATIONS IN JAK2 NUCLEIC ACID
    Publication number: 20190100807
    Abstract: The invention disclosed herein is based on the identification of novel mutations in the JAK2 gene and JAK2 protein. The invention provides compositions and methods useful for diagnosing hematopoietic diseases including, for example, myeloproliferative diseases. The invention also provides compositions and methods useful for determining a prognosis of an individual diagnosed as having a hematopoietic disease.
    Type: Application
    Filed: October 12, 2018
    Publication date: April 4, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventors: Maher Albitar, Wanlong Ma
  • METHODS FOR DETECTING GENE DYSREGULATION BY INTRAGENIC DIFFERENTIAL EXPRESSION
    Publication number: 20190093176
    Abstract: Described herein are methods, compositions and kits directed to the detection of gene dysregulations such as those arising from gene fusions and/or chromosomal abnormalities, e.g., translocations, insertions, inversions and deletions. Samples containing dysregulated gene(s) of interest may show independent expression patterns for the 5? and 3? regions of the gene. The methods, compositions and kits are useful for detecting mutations that cause the differential expression of a 5? portion of a target gene relative to the 3? region of the target gene.
    Type: Application
    Filed: October 5, 2018
    Publication date: March 28, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventors: Heather R. Sanders, Maher Albitar, Aurelia Meloni-Ehrig
  • Mass spectrometric determination of fatty acids
    Patent number: 10242852
    Abstract: The invention relates to the detection of fatty acids. In a particular aspect, the invention relates to methods for detecting very long chain fatty acids and branched chain fatty acids by mass spectrometry.
    Type: Grant
    Filed: August 10, 2016
    Date of Patent: March 26, 2019
    Assignee: Quest Diagnostics Investments Incorporated
    Inventor: Scott Goldman
  • ASSAY FOR DETECTION OF PATHOGENIC LEPTOSPIRA STRAINS
    Publication number: 20190085376
    Abstract: The present invention provides methods and compositions for determining the presence and/or amount of pathogenic Leptospira in a test sample. In particular, substantially purified oligonucleotide primers and probes are described that can be used for qualitatively and quantitatively detecting pathogenic Leptospira nucleic acid in a test sample by amplification methods. The present invention also provides primers and probes for generating and detecting control nucleic acid sequences that provide a convenient method for assessing internal quality control of the Leptospira assay.
    Type: Application
    Filed: April 4, 2016
    Publication date: March 21, 2019
    Applicant: Quest Diagnostics Investiments LLC
    Inventor: Erik P. Johnson
  • METHODS FOR QUANTITATION OF INSULIN AND C-PEPTIDE
    Publication number: 20190079070
    Abstract: Methods are described for diagnosing or prognosing insulin resistance in diabetic and pre-diabetic patients, the method comprising determining the amount of insulin and C-peptide in a sample. Provided herein are mass spectrometric methods for detecting and quantifying insulin and C-peptide in a biological sample utilizing enrichment and/or purification methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques.
    Type: Application
    Filed: March 30, 2018
    Publication date: March 14, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventors: Dov Shiffman, Carmen Tong, James J. Devlin, Michael J. McPhaul
  • HEMATOPOIETIC CELL PHENOTYPING USING CIRCULATING CELL-FREE MARKERS
    Publication number: 20190064170
    Abstract: The present invention provides methods of classifying cluster of differentiation (CD) marker phenotype for hematopoietic cancer cells using multiple circulating cell-free CD markers in bodily fluid. In other aspects, treatment and disease progression of particular hematopoietic cancers can be monitored by measuring the levels of CD and other markers in bodily fluids of a patient.
    Type: Application
    Filed: August 31, 2018
    Publication date: February 28, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventor: Maher Albitar
  • MICRORNA PROFILING FOR DIAGNOSIS OF DYSPLASTIC NEVI AND MELANOMA
    Publication number: 20190062844
    Abstract: Provided herein are methods for miRNA profiling for the diagnosis, prognosis, and management of melanoma and differentiation of melanoma from nevi.
    Type: Application
    Filed: August 20, 2018
    Publication date: February 28, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventors: Kevin Qu, Anthony Sferruzza, Ke Zhang, Yan Liu, Renius Owen
  • RNA ISOLATION FROM SOLUBLE URING FRACTIONS
    Publication number: 20190062726
    Abstract: The invention provides methods for isolating RNA from the soluble fraction of urine. The methods can be used for detecting the presence or absence of an RNA, or quantifying the amount of an RNA. The methods are useful for diagnosing an individual suspected of having a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine. The methods are also useful for prognosing an individual diagnosed with a disease by detecting the level of RNA associated with the disease in the soluble fraction of urine.
    Type: Application
    Filed: August 31, 2018
    Publication date: February 28, 2019
    Applicant: Quest Diagnostics Investments LLC
    Inventors: Heather Sanders, Hai-Rong Li
  • Detection of vitamins A and E by tandem mass spectrometry
    Patent number: 10215765
    Abstract: Methods are described for measuring the amount of one or more of vitamin A, ?-tocopherol, and the combination of ?-tocopherol and ?-tocopherol in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying one or more of vitamin A, ?-tocopherol, and the combination of ?-tocopherol and ?-tocopherol in a sample.
    Type: Grant
    Filed: September 13, 2011
    Date of Patent: February 26, 2019
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Sum Chan, Qibo Jiang
  • Chain of custody forms and methods
    Patent number: 10209265
    Abstract: A chain-of-custody form has integrated removable specimen seals, and the form may be used to identify a test sample collected from a donor and to document the handling of the test sample. The form may be printed upon a single sheet that has a first portion and a second portion. The first portion includes a first section having one or more spaces for recording demographic and testing information, and one or more indicia of the information to be recorded in one or more marked spaces; and a second section having a marked space for the donor to affix a signature and one or more indicia of the purpose of the space. An identifier that is unique to the form is recorded within the first portion and within the second portion. The identifier includes an specimen identification number or some other method of specimen identification such as a bar-code.
    Type: Grant
    Filed: December 19, 2008
    Date of Patent: February 19, 2019
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: R. H. Barry Sample, Eric Quilter
  • Kisspeptin-54 detection by tandem mass spectrometry
    Patent number: 10203337
    Abstract: Methods are described for measuring the amount of a kisspeptin-54-derived peptides in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying a kisspeptin-54 derived peptides in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric techniques.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: February 12, 2019
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Cory E. Bystrom, Richard E. Reitz