Patents Assigned to Raindance Technologies, Inc.
-
Publication number: 20140154695Abstract: The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.Type: ApplicationFiled: February 6, 2014Publication date: June 5, 2014Applicant: Raindance Technologies, Inc.Inventors: Benjamin J. Miller, Qun Zhong, Darren Roy Link
-
Publication number: 20140113300Abstract: The invention generally relates to methods for increasing the amount of DNA available for analysis when using partitioned samples and parallel processing. For example, double-stranded DNA can be dissociated into two single-stranded components, and the single strands partitioned into different droplets prior to analysis. The disclosed methods are useful for performing digital PCR analysis on samples where the target DNA is not in abundance, for example when the sample originates from a body fluid or an FFPE sample.Type: ApplicationFiled: October 21, 2013Publication date: April 24, 2014Applicant: Raindance Technologies, Inc.Inventor: Michael Samuels
-
Publication number: 20140076430Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.Type: ApplicationFiled: September 9, 2013Publication date: March 20, 2014Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
-
Patent number: 8658430Abstract: The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.Type: GrantFiled: July 20, 2012Date of Patent: February 25, 2014Assignee: Raindance Technologies, Inc.Inventors: Benjamin J. Miller, Qun Zhong, Darren Roy Link
-
Patent number: 8535889Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.Type: GrantFiled: February 11, 2011Date of Patent: September 17, 2013Assignee: Raindance Technologies, Inc.Inventors: Jonathan William Larson, Qun Zhong, Darren Roy Link
-
Patent number: 8528589Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.Type: GrantFiled: March 23, 2010Date of Patent: September 10, 2013Assignee: Raindance Technologies, Inc.Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
-
Publication number: 20130109575Abstract: The present invention generally relates to systems and methods to create stable emulsions with low rates of exchange of molecules between microdroplets.Type: ApplicationFiled: December 22, 2010Publication date: May 2, 2013Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Felix Kleinschmidt, Andrew David Griffiths, François Caron, Abdeslam El Harrak, Eric Brouzes, Darren Link
-
Publication number: 20130064776Abstract: The present invention relates to labelled silica-based nanoparticles with enhanced properties, to process for preparing them and to uses thereof.Type: ApplicationFiled: October 11, 2010Publication date: March 14, 2013Applicants: Universite de Strasbourg, RainDance Technologies, Inc., Centre National de la Recherche ScientifiqueInventors: Abdeslam El Harrak, Victoire Goust, Andrew David Griffiths, Thomas Mangeat
-
Publication number: 20120309002Abstract: The invention generally relates to methods for sample multiplexing. In certain embodiments, methods of the invention obtaining a plurality of different reactant molecules, attaching a unique identifier to the reactant molecules, and forming a droplet including the reactant molecules.Type: ApplicationFiled: May 31, 2012Publication date: December 6, 2012Applicant: Raindance Technologies, Inc.Inventor: Darren Link
-
Publication number: 20120302448Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.Type: ApplicationFiled: April 30, 2012Publication date: November 29, 2012Applicant: Raindance Technologies, Inc.Inventors: John Brian Hutchison, Jeffrey Charles Olson, Darren Roy Link
-
Publication number: 20120264646Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.Type: ApplicationFiled: June 1, 2012Publication date: October 18, 2012Applicant: Raindance Technologies, Inc.Inventors: Darren R. Link, Michael L. Samuels
-
Publication number: 20120219947Abstract: The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.Type: ApplicationFiled: February 10, 2012Publication date: August 30, 2012Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Yevgeny Yurkovetsky, Darren Roy Link, Jonathan William Larson
-
Publication number: 20120208241Abstract: The present invention provides thermocycling devices useful for amplification of nucleic acids in droplets. The thermocycling device utilizes the flow of one or more fluids through a main compartment at temperatures sufficient to conduct a polymerase chain reaction. Methods of amplifying nucleic acids in droplets are also provided.Type: ApplicationFiled: February 10, 2012Publication date: August 16, 2012Applicant: RAINDANCE TECHNOLOGIES, INC.Inventor: Darren Roy Link
-
Publication number: 20120122714Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.Type: ApplicationFiled: September 30, 2011Publication date: May 17, 2012Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Michael Samuels, Darren Roy Link
-
Publication number: 20110250597Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.Type: ApplicationFiled: February 11, 2011Publication date: October 13, 2011Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Jonathan William Larson, Qun Zhong, Darren Roy Link
-
Publication number: 20110244455Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.Type: ApplicationFiled: February 11, 2011Publication date: October 6, 2011Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Jonathan William Larson, Qun Zhong, Darren Roy Link
-
Publication number: 20110000560Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.Type: ApplicationFiled: March 23, 2010Publication date: January 6, 2011Applicant: Raindance Technologies, Inc.Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
-
Publication number: 20100022414Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.Type: ApplicationFiled: July 17, 2009Publication date: January 28, 2010Applicant: Raindance Technologies, Inc.Inventors: Darren Link, Brian Hutchison, Michael Samuels, Michael Weiner