Patents Assigned to Salk Institute for Biological Studies
  • Publication number: 20240017097
    Abstract: The invention provides compositions featuring TRP-4 polypeptides and polynucleotides, methods for expressing such polypeptides and polynucleotides in a cell type of interest, and methods for inducing the activation of the TRP-4 polypeptide in neurons and other cell types using ultrasound.
    Type: Application
    Filed: August 31, 2023
    Publication date: January 18, 2024
    Applicant: Salk Institute for Biological Studies
    Inventors: Sreekanth H. CHALASANI, Stuart IBSEN
  • Patent number: 11857809
    Abstract: The invention provides compositions featuring TRP-4 polypeptides and polynucleotides, methods for expressing such polypeptides and polynucleotides in a cell type of interest, and methods for inducing the activation of the TRP-4 polypeptide in neurons and other cell types using ultrasound.
    Type: Grant
    Filed: September 23, 2020
    Date of Patent: January 2, 2024
    Assignee: Salk Institute for Biological Studies
    Inventors: Sreekanth H. Chalasani, Stuart Ibsen
  • Publication number: 20230399663
    Abstract: Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.
    Type: Application
    Filed: May 2, 2023
    Publication date: December 14, 2023
    Applicant: Salk Institute for Biological Studies
    Inventors: Juan Carlos Izpisua Belmonte, Keiichiro Suzuki, Reyna Hernandez-Benitez, Jun Wu, Yuji Tsunekawa
  • Publication number: 20230364263
    Abstract: Synthetic adenoviruses with tropism to bone tissue are described. The synthetic adenoviruses include an adenovirus type 11 (Ad11) fiber protein or a chimeric adenovirus fiber protein having an Ad11 knob domain. The synthetic adenoviruses can also include a transgene, such as a reporter gene or a transgene encoding a factor that promotes bone regeneration or repair. Use of the synthetic adenoviruses to target bone tissue and/or to promote bone repair or regeneration is also described.
    Type: Application
    Filed: April 19, 2023
    Publication date: November 16, 2023
    Applicant: Salk Institute for Biological Studies
    Inventors: Clodagh O'Shea, Colin Powers, Lei Zhang
  • Patent number: 11813337
    Abstract: Synthetic adenoviruses with liver detargeting mutations and expressing an adenovirus type 34 (Ad34) fiber protein, or a chimeric fiber protein with an Ad34 knob domain, are described. The synthetic adenoviruses traffic to sites of tumors. Use of the synthetic adenoviruses for delivering diagnostic or therapeutic transgenes to tumors are also described.
    Type: Grant
    Filed: June 7, 2019
    Date of Patent: November 14, 2023
    Assignee: Salk Institute for Biological Studies
    Inventors: Clodagh O'Shea, Colin Powers, Lei Zhang
  • Publication number: 20230340408
    Abstract: Provided and described are bacterial mechanosensory polypeptide and encoding polynucleotide products and compositions thereof, methods of expressing such polypeptides and polynucleotides in a cell type of interest, and methods of inducing and/or modifying the activity or function of various types of cells, including neurons, which express exogenous bacterial mechanosensory polypeptides, using ultrasound.
    Type: Application
    Filed: May 2, 2023
    Publication date: October 26, 2023
    Applicants: Salk Institute for Biological Studies, The Regents of the University of California
    Inventors: Sreekanth CHALASANI, Corinne LEE-KUBLI, Uri MAGARAM, Daniel GIBBS
  • Publication number: 20230340534
    Abstract: Provided and described are mechanosensory polypeptides and encoding polynucleotide products and compositions thereof, methods of expressing such polypeptides and polynucleotides in a cell type of interest, and methods of inducing and/or modifying the activity and/or function of various types of cells that express the exogenous mechanosensory polypeptides using ultrasound.
    Type: Application
    Filed: May 10, 2023
    Publication date: October 26, 2023
    Applicants: Salk Institute for Biological Studies, The Scripps Research Institute
    Inventors: Sreekanth CHALASANI, Carl PROCKO, Joanne CHORY, Jose Mendoza LOPEZ, Marc Duque RAMIREZ, Corinne LEE-KUBLI, Swetha MURTHY, Ardem PATAPOUTIAN, Seyed Ali Reza MOUSAVI, William T. KEENAN, Yusuf TUFAIL, Janki PATEL
  • Publication number: 20230328020
    Abstract: Provided herein are CRISPR/Cas methods and compositions for targeting RNA molecules, which can be used to detect, edit, or modify a target RNA.
    Type: Application
    Filed: May 26, 2023
    Publication date: October 12, 2023
    Applicant: Salk Institute for Biological Studies
    Inventors: Patrick D. Hsu, Silvana Konermann
  • Patent number: 11760977
    Abstract: The invention features pancreatic islet and pancreatic organoids, and cell cultures and methods that are useful for the rapid and reliable generation of pancreatic islet and pancreatic islet organoids. The invention also features methods of treating pancreatic diseases and methods of identifying agents that are useful for treatment of pancreatic diseases, such as type 2 diabetes and pancreatic cancer, using the pancreatic islet and pancreatic organoids of the invention.
    Type: Grant
    Filed: May 24, 2017
    Date of Patent: September 19, 2023
    Assignee: SALK INSTITUTE FOR BIOLOGICAL STUDIES
    Inventors: Ronald Evans, Michael Downes, Annette Atkins, Eiji Yoshihara, Ruth Yu
  • Publication number: 20230277690
    Abstract: The present disclosure relates to nucleic acid promoter sequences that are able to specifically express genes operatively linked to the promoter in brainstem and spinal motor neuron cells, and to methods for using such promoters to selectively express genes in motor neurons in vitro and in vivo. It is based, at least in part, on the discovery that the nucleic acid of SEQ ID NO: 1 functioned as a motor neuron-specific promoter and was successful in expressing transgenes in motor neuron cells in vivo. The present disclosure also relates to compositions that can increase the activity or expression level of miR-218 and to compositions that can decrease the expression of miR-218 target nucleic acids.
    Type: Application
    Filed: April 7, 2023
    Publication date: September 7, 2023
    Applicant: Salk Institute for Biological Studies
    Inventor: Neal Dilip Amin
  • Publication number: 20230263891
    Abstract: Described herein are compositions featuring TRPA1 polypeptides and polynucleotides, methods for expressing such polypeptides and polynucleotides in a cell type of interest, and methods for inducing the activation of the TRPA1 polypeptide in neurons and other cell types using ultrasound.
    Type: Application
    Filed: July 15, 2021
    Publication date: August 24, 2023
    Applicant: Salk Institute for Biological Studies
    Inventors: Sreekanth CHALASANI, Yusuf TUFAIL, Jose Mendoza LOPEZ, Marc Duque RAMIREZ, Uri MAGARAM, Corinne LEE-KUBLI, Eric Warren EDSINGER
  • Patent number: 11730781
    Abstract: Synthetic adenoviruses having chimeric fiber proteins and liver detargeting mutations are described. The synthetic adenovirus vectors are capable of specifically infecting cells at wound sites or in regions of damaged tissue. The synthetic adenovirus vectors also are capable of expressing transgenes, such as wound healing factors, at sites of wounded or damaged tissue. Accordingly, the described vectors can be used to detect wounded or damaged tissue, and/or to promote wound healing and regeneration of damaged tissue, such as by expression of heterologous wound healing or tissue regeneration factors.
    Type: Grant
    Filed: April 4, 2018
    Date of Patent: August 22, 2023
    Assignee: Salk Institute for Biological Studies
    Inventors: Clodagh O'Shea, Colin Powers, Lei Zhang
  • Patent number: 11706177
    Abstract: Provided herein are CRISPR/Cas methods and compositions for targeting RNA molecules, which can be used to detect, edit, or modify a target RNA.
    Type: Grant
    Filed: April 1, 2022
    Date of Patent: July 18, 2023
    Assignee: Salk Institute for Biological Studies
    Inventors: Patrick D. Hsu, Silvana Konermann
  • Publication number: 20230220355
    Abstract: Recombinant adenovirus genomes that include a synthetic transcriptional circuit are described. Synthetic adenoviruses positively regulated using two-step transcriptional amplification (TSTA) are further described. Selection of the heterologous promoter is based on the desired replication characteristics of the synthetic virus. For example, the heterologous promoter can be a constitutive promoter, a tumor-specific promoter or a tissue-specific promoter.
    Type: Application
    Filed: January 6, 2023
    Publication date: July 13, 2023
    Applicant: Salk Institute for Biological Studies
    Inventors: William Partlo, Clodagh O'Shea
  • Publication number: 20230218690
    Abstract: Oncolytic adenoviruses capable of selectively replicating in tumor cells deficient in p53 transcriptional activity are described. Recombinant adenovirus genomes that encode the p53-selective adenoviruses are also described. The recombinant adenoviruses and adenovirus genomes, or compositions thereof, can be used, for example, to reduce or inhibit tumor progression, or reduce tumor volume in a subject with a tumor having dysregulated p53 activity.
    Type: Application
    Filed: January 6, 2023
    Publication date: July 13, 2023
    Applicant: Salk Institute for Biological Studies
    Inventors: William Partlo, Clodagh O'Shea
  • Patent number: 11690557
    Abstract: Determining low power frequency range information from spectral data. Raw signal data can be adjusted to increase dynamic range for power within low power frequency ranges as compared to higher-power frequency ranges to determine adjusted source data valuable for acquiring low power frequency range information. Low power frequency range information can be used in the analysis of a variety of raw signal data. For example, low power frequency range information within electroencephalography data for a subject from a period of sleep can be used to determine sleep states. Similarly, automated full-frequency spectral electroencephalography signal analysis can be useful for customized analysis including assessing sleep quality, detecting pathological conditions, and determining the effect of medication on sleep states.
    Type: Grant
    Filed: July 31, 2019
    Date of Patent: July 4, 2023
    Assignee: The Salk Institute For Biological Studies Intellectual Property And Technology Transfer
    Inventor: Philip Low
  • Patent number: 11685901
    Abstract: The invention features pancreatic islet and pancreatic organoids, and cell cultures and methods that are useful for the rapid and reliable generation of pancreatic islet and pancreatic islet organoids. The invention also features methods of treating pancreatic diseases and methods of identifying agents that are useful for treatment of pancreatic diseases, such as type 2 diabetes and pancreatic cancer, using the pancreatic islet and pancreatic organoids of the invention.
    Type: Grant
    Filed: March 28, 2022
    Date of Patent: June 27, 2023
    Assignee: Salk Institute for Biological Studies
    Inventors: Ronald Evans, Michael Downes, Annette Atkins, Eiji Yoshihara, Ruth Yu
  • Patent number: 11674158
    Abstract: Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.
    Type: Grant
    Filed: July 14, 2017
    Date of Patent: June 13, 2023
    Assignee: Salk Institute for Biological Studies
    Inventors: Juan Carlos Izpisua Belmonte, Keiichiro Suzuki, Reyna Hernandez-Benitez, Jun Wu, Yuji Tsunekawa
  • Patent number: 11642423
    Abstract: The present disclosure relates to nucleic acid promoter sequences that are able to specifically express genes operatively linked to the promoter in brainstem and spinal motor neuron cells, and to methods for using such promoters to selectively express genes in motor neurons in vitro and in vivo. It is based, at least in part, on the discovery that the nucleic acid of SEQ ID NO: 1 functioned as a motor neuron-specific promoter and was successful in expressing transgenes in motor neuron cells in vivo. The present disclosure also relates to compositions that can increase the activity or expression level of miR-218 and to compositions that can decrease the expression of miR-218 target nucleic acids.
    Type: Grant
    Filed: June 16, 2020
    Date of Patent: May 9, 2023
    Assignee: Salk Institute for Biological Studies
    Inventor: Neal Dilip Amin
  • Publication number: 20230111083
    Abstract: Provided are methods for identification of DNA repair locations in a genome of a non-dividing cell, by incorporating a reactive nucleoside analogs into the genome of the non-dividing cell, then sequencing the regions of the genome that incorporated the nucleoside analog.
    Type: Application
    Filed: June 15, 2020
    Publication date: April 13, 2023
    Applicant: SALK INSTITUTE FOR BIOLOGICAL STUDIES
    Inventors: Fred H. Gage, Dylan A. Reid, Patrick J. Reed