Abstract: The present invention relates to novel strains of microorganisms with oxygen-regulated metabolism. The microorganisms have higher growth rates and are more efficient than parental strains. The microorganisms may be used to produce a variety of products of interests, such as recombinant proteins, nucleic acids, such as DNA, amino acids, and chemicals.
Type:
Grant
Filed:
September 8, 2006
Date of Patent:
December 2, 2014
Assignee:
Scarab Genomics, LLC
Inventors:
Frederick R. Blattner, John Walter Campbell, Nian Shi, Buffy Stahl
Abstract: Reduced genome bacteria with improved genetic stability are provided. Also provided are methods of producing polypeptides using the reduced genome bacteria with improved genetic stability.
Type:
Application
Filed:
October 19, 2012
Publication date:
October 9, 2014
Applicant:
SCARAB GENOMICS, LLC
Inventors:
Frederick R. Blattner, Balint Csorgo, Gyorgy Posfai
Abstract: The present invention relates generally to compositions and methods useful for, inter alia, production of commercial biologic products such as amino acids. More specifically, the present invention relates to genetically modified strains of microorganisms and the use thereof for the production of commercial products. The present invention also provides, inter alia, novel isolated DNA, nucleic acid, vectors and reduced genome bacteria.
Type:
Grant
Filed:
February 19, 2009
Date of Patent:
February 5, 2013
Assignee:
Scarab Genomics LLC
Inventors:
Frederick R. Blattner, Sun Chang Kim, Jun Hyoung Lee
Abstract: Reduced genome strains of E. coli MG1 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
Type:
Application
Filed:
April 12, 2012
Publication date:
January 10, 2013
Applicant:
SCARAB GENOMICS, LLC
Inventors:
John W. Campbell, Frederick R. Blattner, Guy Plunkett, Gyorgy Posfai
Abstract: Reduced genome strains of E. coli MG1655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
Type:
Grant
Filed:
August 18, 2006
Date of Patent:
May 15, 2012
Assignee:
Scarab Genomics LLC
Inventors:
John W Campbell, Frederick R. Blattner, Guy Plunkett, Gyorgy Posfai
Abstract: Methods for prophylaxis or treatment of sepsis in a subject are provided comprising administering to the subject a therapeutically effective dose of multiple deletion strain bacteria.
Type:
Application
Filed:
February 10, 2010
Publication date:
April 19, 2012
Applicant:
SCARAB GENOMICS LLC
Inventors:
Frederick Blattner, Valerie Burland, John Campbell, Charles Landry
Abstract: Methods for prophylaxis or treatment of sepsis in a subject are provided comprising administering to the subject a therapeutically effective dose of multiple deletion strain bacteria.
Type:
Application
Filed:
February 10, 2010
Publication date:
December 1, 2011
Applicant:
SCARAB GENOMICS LLC
Inventors:
Frederick R. Blattner, Valerie Burland, John Walter Campbell, Charles Landry
Abstract: The invention relates to host cells with improved protein expressed properties. The host cells comprise rare tRNA genes within the one or more rRNA operons.
Type:
Grant
Filed:
April 23, 2007
Date of Patent:
August 2, 2011
Assignee:
Scarab Genomics, LLC
Inventors:
Frederick R. Blattner, John Walter Campbell, Guy Plunkett
Abstract: Methods for introducing and expressing genes in animal cells are provided comprising infecting the animal cells with live invasive reduced-genome bacteria comprising a eukaryotic expression cassette comprising said gene. Also provided are methods for producing a pluripotent stem (iPS) cell from a mammalian somatic cell comprising infecting the somatic cell with live invasive reduced-genome bacteria comprising one or more eukaryotic expression cassettes comprising at least a gene encoding the transcription factor Oct3/4 and a gene encoding a member of the SRY-related HMG-box (Sox) transcription factor family.
Type:
Application
Filed:
September 14, 2009
Publication date:
July 7, 2011
Applicant:
Scarab Genomics, LLC
Inventors:
Frederick R. Blattner, Val Burland, Charles Landy, Nian-Qing Shi, Buffy Stahl
Abstract: The various embodiments of the present invention relate generally to plasmid DNA preparations and to methods for producing and using such preparations.
Type:
Grant
Filed:
May 24, 2007
Date of Patent:
May 3, 2011
Assignee:
Scarab Genomics, LLC
Inventors:
Frederick R. Blattner, John Walter Campbell, Guy Plunkett
Abstract: Provided herein is a nucleic acid comprising a mutant lac operator operably linked to a gene of interest, a host cell comprising the nucleic acid, and a method of using the host cell to express the gene of interest. Also provided is a recA-mediated cloning method.
Type:
Application
Filed:
November 26, 2008
Publication date:
March 3, 2011
Applicant:
SCARAB GENOMICS LLC
Inventors:
Frederick Blattner, Dimitry Schevchenko, David Frisch, John Campbell
Abstract: The present invention relates generally to compositions and methods useful for, inter alia, production of commercial biologic products such as amino acids. More specifically, the present invention relates to genetically modified strains of microorganisms and the use thereof for the production of commercial products. The present invention also provides, inter alia, novel isolated DNA, nucleic acid, vectors and reduced genome bacteria.
Type:
Application
Filed:
February 19, 2009
Publication date:
December 30, 2010
Applicant:
SCARAB GENOMICS LLC
Inventors:
Frederick R. Blattner, Sun Chang Kim, Jun Hyoung Lee
Abstract: The various embodiments of the present invention relate generally to plasmid DNA preparations and to methods for producing and using such preparations.
Type:
Application
Filed:
May 24, 2007
Publication date:
March 12, 2009
Applicant:
SCARAB GENOMICS, LLC
Inventors:
Frederick R. Blattner, John Walter Campbell, Guy Plunkett
Abstract: The present invention relates to novel strains of microorganisms with oxygen-regulated metabolism. The microorganisms have higher growth rates and are more efficient than parental strains. The microorganisms may be used to produce a variety of products of interests, such as recombinant proteins, nucleic acids, such as DNA, amino acids, and chemicals.
Type:
Application
Filed:
September 8, 2006
Publication date:
February 26, 2009
Applicant:
SCARAB GENOMICS, LLC
Inventors:
Frederick R. Blattner, John Walter Campbell, Nian Shi, Buffy Stahl
Abstract: The present invention discloses that a bacterium having a genome that is genetically engineered to be at least 10% smaller than the genome of its native parent strain has better transformation competence. Specific E. coli strains, having significantly reduced genome sizes, are disclosed which are highly transformation competent. A medium and methodology is taught which enables transformation efficiencies to be increased further.
Type:
Grant
Filed:
September 5, 2003
Date of Patent:
December 4, 2007
Assignees:
Wisconsin Alumni Research Foundation, Scarab Genomics, LLC
Inventors:
Frederick R. Blattner, Gyorgy Posfai, Christopher D. Herring, Guy Plunkett, III, Jeremy Glasner, Trevor Twose