Patents Assigned to Seikagaku Kogyo Kabushiki Kaisha
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Patent number: 5548077Abstract: 2-Aminopyridine derivatives having a bifunctional or unifunctional structure and capable of serving as reactants in synthetically converting organic compounds, such as carbohydrates, proteins, lipids or polymer resins, to desired conjugated structures suited for various purposes and detectable by fluorescence spectrometry with high sensitivity, a fluorescent labeling agent comprising the same, and methods of their production are disclosed.Type: GrantFiled: September 23, 1994Date of Patent: August 20, 1996Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Shoichi Kusumoto, Koichi Fukase, Sumihiro Hase
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Patent number: 5496718Abstract: A crystallizable, purified chondroitinase ABC having a molecular weight of about 100,000 dalton by the measurement of the SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and the measurement by the gel permeation chromatography method, having alanine as the N-terminal amino acid and proline as the C-terminal amino acid. A process for the purification of the crystallizable purified chondroitinase ABC comprising removing nucleic acid from an surfactant solution extract obtained from cells of chondroitinase ABC-producing microorganisms and chromatographically treating by concentration gradient elution using a weak cation exchange resin or a strong cation exchange resin. A composition comprising a chondroitinase and serum albumin, gelatin, or a nonionic surfactant. The chondroitinase ABC is isolated from Proteus vulgaris ATCC 6896.Type: GrantFiled: June 23, 1993Date of Patent: March 5, 1996Assignee: Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)Inventors: Nobukazu Hashimoto, Hideo Mochizuki, Akio Hamai
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Patent number: 5470578Abstract: This invention provides an antirheumatic composition which comprises a lipid-bound glycosaminoglycan or a salt thereof as an active ingredient and a pharmaceutically acceptable carrier and has an effect to inhibit extension of pannus.Type: GrantFiled: July 29, 1993Date of Patent: November 28, 1995Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Shigehisa Aoki, Shinichi Iwasaki, Nobuo Sugiura, Sakaru Suzuki, Koji Kimata
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Patent number: 5468232Abstract: A cartridge-type syringe in which a cartridge, having liquid medicine or the like sealed therein, is inserted in a holder having a discharge needle at its distal end, and the cartridge is advanced to cause the discharge needle to pierce it, thereby injecting the liquid medicine or the like into an object, and when a piston rod of the cartridge is pushed to cause the discharge needle to pierce, the liquid medicine or the like will not be discharged in error in a large amount.In particular there is provided a releaseable lock mechanism by which when a piston rod 15 is pushed, the piston rod 15 advances together with a tubular body 11 of a cartridge 10 without moving a piston 14 within the tubular body 11.Type: GrantFiled: January 27, 1993Date of Patent: November 21, 1995Assignee: Seikagaku Kogyo Kabushiki Kaisha(Seikagaku Corporation)Inventor: Masateru Naganuma
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Patent number: 5464942Abstract: This invention relates to compounds prepared by linking glycosaminoglycan to phospholipid or lipid, which are expected to exert a pharmacological effect for inhibiting metastasis because of their excellent function to inhibit adhesion of cancer cells to blood vessel endothelial cells and extracellular matrix. This phospholipid- or lipid-linked glycosaminoglycan can be produced for example by: cleaving and oxidizing reducing terminal group of glycosaminoglycan, and allowing an aldehyde group or a lactone compound of the thus-formed derivative or a carboxyl group in the glycosaminoglycan chain to react with a primary amino group of a phospholipid; or linking a glycosaminoglycan derivative to a phospholipid or a lipid by allowing a primary amino group of the derivative to react with a carboxyl group of the phospholipid or lipid. This phospholipid- or lipid-linked glycosaminoglycan is useful as a metastasis inhibitor because it has no toxicity.Type: GrantFiled: March 24, 1991Date of Patent: November 7, 1995Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Katsukiyo Sakurai, Nobuo Sugiura, Koji Kimata, Sakaru Suzuki
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Patent number: 5462976Abstract: Photocurable glycosaminoglycan (GAG) derivatives and crosslinked glycosaminoglycans, which are highly safe and biocompatible, a method of preparing such photocurable GAG derivatives readily moldable by casting using a solvent when desired, by which method unreacted substances causative of adverse effects can be readily eliminated, and a method of producing the crosslinked GAGs and medical materials based on the photocurable GAG derivatives or crosslinked GAGs are provided. The photocurable GAG derivatives comprise a glycosaminoglycan and a photoreactive compound bound thereto and can be produced, for example, by subjecting hydroxyl or carboxyl groups of the glycosaminoglycan to esterification reaction or amidation reaction, respectively, with the photoreactive compound. The crosslinked GAGs are derived from the photocurable GAG derivatives by intermolecular crosslinking of the photoreactive compound bound thereto.Type: GrantFiled: February 5, 1993Date of Patent: October 31, 1995Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Takehisa Matsuda, Minoo J. Moghaddam, Katsukiyo Sakurai
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Patent number: 5405759Abstract: Disclosed are novel enzymes, heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV, which degrade heparan sulfate and/or heparin, a process for producing thereof by cultivating a novel Bacillus circulans HpT 298 having an ability of producing these enzymes and a novel Bacillus circulans HpT 298.Type: GrantFiled: November 18, 1993Date of Patent: April 11, 1995Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Kiyoshi Morikawa, Hirofumi Miyazono, Hiroshi Maruyama, Keiichi Yoshida
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Patent number: 5401647Abstract: Disclosed is a method of preparing limulus amoebocyte lysate substantially free from factor G which comprises bringing limulus amoebocyte lysate into contact with an insoluble carrier on which a (1.fwdarw.3)-.beta.-D-glucoside structural portion represented by the following formula [I] produced by depolymerizing and/or fractionating a carbohydrate chain is immobilized: ##STR1## wherein n represents an integer of 2 to 370.Type: GrantFiled: May 27, 1992Date of Patent: March 28, 1995Assignee: Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)Inventors: Shigenori Tanaka, Jun Aketagawa, Yuko Shibata
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Patent number: 5389547Abstract: A reagent denatures or eliminates factors interfering with biochemical reactions by simple treatment without requiring separation of any denatured product precipitate. The reagent makes it possible to assay, in particular, .beta.-glucan and endotoxin in blood-derived samples rapidly and efficiently with high sensitivity. The reagent includes a hexadimethrine compound and an alkali metal hydroxide or an alkali metal hydroxide as a main component. A method for assaying a substance specifically reacting with a Limulus reagent utilizing the reagent, an assay kit including at least the reagent and a Limulus reagent, and a method of diagnosing infectious diseases based on the results obtained by the assay method are also provided.Type: GrantFiled: May 10, 1993Date of Patent: February 14, 1995Assignee: Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)Inventors: Shigenori Tanaka, Hiroshi Tamura
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Patent number: 5386033Abstract: 2-Aminopyridine derivatives having a bifunctional or unifunctional structure and capable of serving as reactants in synthetically converting organic compounds, such as carbohydrates, proteins, lipids or polymer resins, to desired conjugated structures suited for various purposes and detectable by fluorescence spectrometry with high sensitivity, a fluorescent labeling agent comprising the same, and methods of their production are disclosed.Type: GrantFiled: March 15, 1993Date of Patent: January 31, 1995Assignee: Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)Inventors: Shoichi Kusumoto, Sumihiro Hase, Koichi Fukase
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Patent number: 5378610Abstract: The present invention has been made in order to solve the problems in conventional methods of assaying an endotoxin in a specimen such as plasma or serum by the limulus test, which requires a complicated pretreatment procedure such as centrifugation for removing denatured precipitates formed with an acid treatment. According to the assay method of the present invention, an endotoxin adsorbed by proteins, lipids and platelets can be efficiently liberated simply by adding a mixed aqueous solution having a specific composition according to the present invention without any separation procedure and thus a sample solution of good qualities can be prepared. After adding the mixed aqueous solution containing a specific surfactant, a compound having an imidazolyl group or an amino group and an alkaline earth metal salt and an alkaline metal hydroxide to the specimen, followed by addition of the limulus amoebocyte lysate components, the endotoxin can be easily, quickly and precisely assayed.Type: GrantFiled: May 28, 1992Date of Patent: January 3, 1995Assignee: Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)Inventors: Shigenori Tanaka, Hiroshi Tamura
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Patent number: 5307144Abstract: The present invention intends to generally maintain the microplate having added test samples in wells therein at even temperature for measuring biochemical or biological reaction accompanying changes in absorbance such as enzyme reaction. Monochromatic lights with various wave lengths from a light source 3 are transmitted through the test samples added to a plurality of wells in a microplate 1 to measure absorbance of the test sample. For this end, a metal plate 2 with good heat conductivity is substantially contacted with the bottom of each well in the microplate 1 and a perforated board 7 is disposed with an air space over the microplate 1 at the starting position thereof. Warmed air heated by a heater 17 is blown onto the microplate 1 through the perforated board 7 and is circulated along the metal plate 2 within a chamber 9.Type: GrantFiled: December 1, 1992Date of Patent: April 26, 1994Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Tamura Hiroshi, Tanaka Shigenori
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Patent number: 5290695Abstract: Disclosed are novel enzymes, heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV, which degrade heparan sulfate and/or heparin, a process for producing thereof by cultivating a novel Bacillus circulans HpT 298 having an ability of producing these enzymes and a novel Bacillus circulans HpT 298.Type: GrantFiled: February 28, 1992Date of Patent: March 1, 1994Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Kiyoshi Morikawa, Hirofumi Miyazono, Hiroshi Maruyama, Keiichi Yoshida