Abstract: The invention relates to the technical field of agricultural planting, in particular to a special controlled-release microbial fertilizer for peanuts in a continuous cropping field and a preparation method thereof. The fabric comprises an outer layer, a middle layer and an inner layer. Through a three-layer structure, the controlled-release fertilizer prepared by the disclosure can realize the fertilizer efficiency period of 50-60 days, and achieves the purpose of twice releases.

Abstract: The present disclosure provides a joint control method for nitrogen and phosphorus emissions in farmlands, comprising: reducing nitrogen and phosphorus input during crop sowing or planting by applying composite organic material and chemical fertilizer, wherein the composite organic material comprises: 200-250 parts of edible fungi residues, 300-350 parts of charcoal and 5-10 parts of rhamnolipid; constructing a nitrogen-phosphorus retention layer by utilizing composite microbial agent in combination with 150 parts of edible fungi residues and 20 parts of straw-based hydrogel; constructing a barrier layer by utilizing composite material, and controlling downward leaching of nitrogen and phosphorus that are not absorbed by crops, wherein the composite material of the barrier layer comprises: 25-35 parts of straw-based hydrogel, 20-30 parts of edible fungi residues, 35-55 parts of bentonite and 5-10 parts of corn flour.

Abstract: The invention relates to the technical field of agricultural planting, in particular to a controlled-release bacterial fertilizer for peanuts and a preparation method of the special controlled-release bacterial fertilizer. The special controlled-release bacterial fertilizer is composed of an inner layer and an outer layer; after the outer layer is released completely, the inner-layer network structure gradually absorbs water and preserves moisture, and has good slow release and controlled release properties, thereby achieving the gradual release of the inner layer, high fertilizer utilization rate, exerting the efficacy of the microbial agent and facilitating the large-area promotion.

Abstract: The present disclosure provides a joint control method for nitrogen and phosphorus emissions in farmlands, comprising: reducing nitrogen and phosphorus input during crop sowing or planting by applying composite organic material and chemical fertilizer, wherein the composite organic material comprises: 200-250 parts of edible fungi residues, 300-350 parts of charcoal and 5-10 parts of rhamnolipid; constructing a nitrogen-phosphorus retention layer by utilizing composite microbial agent in combination with 150 parts of edible fungi residues and 20 parts of straw-based hydrogel; constructing a barrier layer by utilizing composite material, and controlling downward leaching of nitrogen and phosphorus that are not absorbed by crops, wherein the composite material of the barrier layer comprises: 25-35 parts of straw-based hydrogel, 20-30 parts of edible fungi residues, 35-55 parts of bentonite and 5-10 parts of corn flour.

Abstract: The present invention discloses an “inferior-eliminating and superior-selecting” breeding method for synergistically improving wheat yield and quality. A method for breeding target wheat varieties with improved yield and quality is provided herein, which combines the advantages of pedigree method and hybrid method, and provides a method for selection of all generations using molecular marker in combination with phenotype identification, and for selection of each generation according to different standards. Different from the derivative system method, the method reserves the traceability of line history and genetic relationship and the characteristics of simple operation and abundant genetic diversity of the hybrid method.

Abstract: The present disclosure discloses a mutant of a lycopene epsilon cyclase (Lcye) gene crucial in a wheat carotenoid synthesis pathway and use thereof. The present disclosure provides the following proteins: (1) a protein obtained by substituting serine at position 253 of an Lcye-D1 protein with phenylalanine; (2) a derived protein that is obtained by subjecting the protein in (1) to substitution and/or deletion and/or addition of one or more amino acid residues and has the same ability as the protein in (1); (3) a protein that has a homology of more than 99%, more than 95%, more than 90%, more than 85%, or more than 80% with the amino acid sequence defined in any one of (1) and (2) and has the same function as the amino acid sequence; and (4) a fusion protein obtained by attaching a tag to N-terminus and/or C-terminus of the protein in any one of (1) to (3).

Abstract: The present disclosure provides a joint control method for nitrogen and phosphorus emissions in farmlands, comprising: reducing nitrogen and phosphorus input during crop sowing or planting by applying composite organic material and chemical fertilizer, wherein the composite organic material comprises: 200-250 parts of edible fungi residues, 300-350 parts of charcoal and 5-10 parts of rhamnolipid; constructing a nitrogen-phosphorus retention layer by utilizing composite microbial agent in combination with 150 parts of edible fungi residues and 20 parts of straw-based hydrogel; constructing a barrier layer by utilizing composite material, and controlling downward leaching of nitrogen and phosphorus that are not absorbed by crops, wherein the composite material of the barrier layer comprises: 25-35 parts of straw-based hydrogel, 20-30 parts of edible fungi residues, 35-55 parts of bentonite and 5-10 parts of corn flour.

Abstract: The invention relates to the technical field of agricultural planting, in particular to a special controlled-release microbial fertilizer for peanuts in a continuous cropping field and a preparation method thereof. The fabric comprises an outer layer, a middle layer and an inner layer. Through a three-layer structure, the controlled-release fertilizer prepared by the disclosure can realize the fertilizer efficiency period of 50-60 days, and achieves the purpose of twice releases.

Abstract: The invention relates to the technical field of agricultural planting, in particular to a controlled-release bacterial fertilizer for peanuts and a preparation method of the special controlled-release bacterial fertilizer. The special controlled-release bacterial fertilizer is composed of an inner layer and an outer layer; after the outer layer is released completely, the inner-layer network structure gradually absorbs water and preserves moisture, and has good slow release and controlled release properties, thereby achieving the gradual release of the inner layer, high fertilizer utilization rate, exerting the efficacy of the microbial agent and facilitating the large-area promotion.

Abstract: The present disclosure provides a joint control method for nitrogen and phosphorus emissions in farmlands, comprising: reducing nitrogen and phosphorus input during crop sowing or planting by applying composite organic material and chemical fertilizer, wherein the composite organic material comprises: 200-250 parts of edible fungi residues, 300-350 parts of charcoal and 5-10 parts of rhamnolipid; constructing a nitrogen-phosphorus retaintion layer by utilizing composite microbial agent in combination with 150 parts of edible fungi residues and 20 parts of straw-based hydrogel; constructing a barrier layer by utilizing composite material, and controlling downward leaching of nitrogen and phosphorus that are not absorbed by crops, wherein the composite material of the barrier layer comprises: 25-35 parts of straw-based hydrogel, 20-30 parts of edible fungi residues, 35-55 parts of bentonite and 5-10 parts of corn flour.

Abstract: A one-off fertilization method suitable for winter wheat in areas with less rainfall in spring includes the following steps: (1) carrying out, on a flat wheat block which is suitable for mechanized operations and after the harvest of preceding crops, a deep ploughing or a subsoiling in combination with a rotary tillage and a one-off fertilization for land preparation, without involving any top dressing in a later stage; and (2) carrying out a seeding operation by a wheat seeder, wherein for nitrogen fertilizer, according to the nitrogen release period of a controlled-release nitrogen fertilizer, one of the following application modes is determined: applying a polyurethane-coated controlled-release nitrogen fertilizer alone or a water-based resin-coated controlled-release nitrogen fertilizer special for winter wheat alone; or applying a water-based resin-coated controlled-release nitrogen fertilizer special for winter wheat in combination with 20% to 30% of a polyurethane-coated controlled-release nitrogen fer

Abstract: The present invention relates to porcine reproductive and respiratory syndrome virus (PRRSV) vaccines in a field of veterinary biological products, and more particularly to a PRRSV SX-105 strain having a deposit number of CGMCC No. 9906, and a use thereof in preparing PRRSV live vaccines. PRRSV SX-1 original strains are strongly pathogenic to swine. A PRRSV SX-105 attenuated strain shows safety and good immunogenicity on the swine. The PRRSV live vaccines, prepared from the PRRSV SX-105 strain, are safe and reliable, and able to generate strong immunity after immunization. The PRRSV live vaccines significantly decrease morbidity and mortality in inoculated swine groups, and have immunization effects reaching and slightly better than conventional commercial vaccines on the market, showing advantages to compete with the same kind of products around the world. The PRRSV live vaccines are capable of effectively preventing PRRS epidemic and transmission, and has broad application prospects.

Abstract: The invention relates to the field of controlled release fertilizer technology, and in particular to a special film-coated controlled release fertilizer for peanut in a high yield field, which comprises an outer layer, an intermediate layer and an inner layer, integrates the ingredients for disease and pest control, chemical control, growth promotion and premature senescence prevention, controls the release period, improves the fertilization efficiency without the need of top application throughout the growth period, saves labor cost, and has positive effects on improving peanut yield and quality.

Abstract: A special film-coated controlled release calcium fertilizer for peanut includes a three-layer structure. Raw materials in an inner layer include calcium nitrate, humic acid, seaweed extract and adhesive. Raw materials in an intermediate layer include calcium nitrate, urea formaldehyde powder and chitosan oligosaccharide. Raw materials in an outer layer include urea formaldehyde powder and fermented livestock and poultry manure. The special controlled release calcium fertilizer for peanut releases Ca since the pod-bearing stage after the basal application. The coating film is a biodegradable film, releases calcium since about 50 days after application into soil.

Abstract: A cotton molecular breeding method includes (1) obtaining a chr.7 single QTL segment substitution line with high fiber strength, and (2) using the obtained chr.7 single QTL segment substitution line as a non-recurrent parent () to cross with a recurrent parent (?) to obtain an F1 generation. Then (3) the F1 generation is back-crossed with the recurrent parent to obtain a BC1F1 generation, and (4) plants of the BC1F1 generation are screened for plants carrying the chr.7 single QTL segment, and then the screened plants are back-crossed with the recurrent parent for 2-4 times to obtain a BCnF1 generation. The method also includes (5) screening plants of the BCnF1 generation for plants carrying the chr.7 single QTL segment, and selfing the screened plants for 1-3 times to obtain a bred line BCnFm having the high cotton fiber strength.

Abstract: The invention relates to the field of controlled release fertilizer technology, and in particular to a special film-coated controlled release fertilizer for peanut in saline-alkali soil, which comprises an outer layer, an intermediate layer and an inner layer, integrates the ingredients for salt resistance improvement, etiolated seedling prevention, disease and pest control, growth promotion and pod plumpness promotion, controls the release period, improves the fertilization efficiency without the need of top application throughout the growth period, and saves labor cost.

Abstract: The invention relates to the field of controlled release fertilizer technology, and in particular to a special film-coated controlled release fertilizer for peanut in a high yield field, which comprises an outer layer, an intermediate layer and an inner layer, integrates the ingredients for disease and pest control, chemical control, growth promotion and premature senescence prevention, controls the release period, improves the fertilization efficiency without the need of top application throughout the growth period, saves labor cost, and has positive effects on improving peanut yield and quality.

Abstract: A special film-coated controlled release calcium fertilizer for peanut includes a three-layer structure. Raw materials in an inner layer include calcium nitrate, humic acid, seaweed extract and adhesive. Raw materials in an intermediate layer include calcium nitrate, urea formaldehyde powder and chitosan oligosaccharide. Raw materials in an outer layer include urea formaldehyde powder and fermented livestock and poultry manure. The special controlled release calcium fertilizer for peanut releases Ca since the pod-bearing stage after the basal application. The coating film is a biodegradable film, releases calcium since about 50 days after application into soil.

Abstract: An application of thiolated single-stranded DNA enhances specific amplification of polymerase chain reaction, namely a method for enhancing specific amplification of polymerase chain reaction by utilizing thiolated single-stranded DNA, which includes the following step: adding an appropriate amount of the thiolated single-stranded DNA into a PCR system to perform PCR amplification, wherein the appropriate amount means that the final concentration of the thiolated single-stranded DNA in a 20 ?L reaction system is not less than 15 ?M. The thiolated single-stranded DNA meets the following conditions: the thiolated single-stranded DNA is one segment of any sequence which is non-complementary and non-homologous to a target sequence; the Tm value is not less than 37.7° C.; and at least one end contains a thiolalkyl group SH—C6H12—.

Abstract: A BrTCP24 gene for controlling the growth of Chinese cabbage and its applications. The BrTCP24 gene has the nucleotide sequence shown in SEQ ID No. 1, the whole length of which is 1221 bp. The gene is useful for improving the production of economic crop. As indicated by experimental results, the gene serves as a controlling gene for plant growth and has the function of down-regulating the plant growth. Through overexpressing the gene, a transgenic plant smaller than the wild one can be obtained. The gene of the invention can provide theory basis and gene source for breeding new varieties of crops.