Patents Assigned to Sigma-Aldrich Co.
  • SHORT RNA MIMETICS
    Publication number: 20120184726
    Abstract: The present invention provides synthetic oligonucleotides that mimic the function of short RNAs such as, for example, microRNAs or short interfering RNAs. In particular, the synthetic oligonucleotides comprise a duplex region comprising an unpaired bulge in one of the strands.
    Type: Application
    Filed: September 13, 2010
    Publication date: July 19, 2012
    Applicant: SIGMA ALDRICH CO. LLC
    Inventor: Kevin Forbes
  • Methods of atomic layer deposition using titanium-based precursors
    Patent number: 8221852
    Abstract: Methods of forming titanium-containing films by atomic layer deposition are provided. The methods comprise delivering at least one precursor to a substrate, wherein the at least one precursor corresponds in structure to Formula I: wherein: R is C1-C6-alkyl; n is zero, 1, 2, 3, 4 or 5; L is C1-C6-alkoxy or amino, wherein the amino is optionally independently substituted 1 or 2 times with C1-C6-alkyl.
    Type: Grant
    Filed: September 10, 2008
    Date of Patent: July 17, 2012
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Peter Nicholas Heys, Andrew Kingsley, Fuquan Song, Paul Williams, Thomas Leese, Hywel Owen Davies, Rajesh Odedra
  • HIGH MOLECULAR WEIGHT ALKYL-ALLYL COBALTTRICARBONYL COMPLEXES AND USE THEREOF FOR PREPARING DIELECTRIC THIN FILMS
    Publication number: 20120177845
    Abstract: A method for forming a cobalt-containing thin film by a vapor deposition process is provided. The method comprises using at least one precursor corresponding in structure to Formula (I); wherein R1 and R2 are independently C2-C8-alkyl; x is zero, 1 or 2; and y is zero or 1; wherein both x and y can not be zero simultaneously.
    Type: Application
    Filed: July 27, 2010
    Publication date: July 12, 2012
    Applicant: Sigma-Aldrich Co. LLC
    Inventors: Rajesh Odedra, Neil Boag, Jeff Anthis, Ravi Kanjolia
  • GENOMIC EDITING OF GENES INVOLVED IN MACULAR DEGENERATION
    Publication number: 20120159653
    Abstract: The present invention provides genetically modified animals and cells comprising edited chromosomal sequences encoding proteins associated with MD. In particular, the animals or cells are generated using a zinc finger nuclease-mediated editing process. Also provided are methods of using the genetically modified animals or cells disclosed herein to study MD development and methods of assessing the effects of agents in genetically modified animals and cells comprising edited chromosomal sequences encoding proteins associated with MD.
    Type: Application
    Filed: July 23, 2010
    Publication date: June 21, 2012
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Edward Weinstein, Xiaoxia Cui, Phil Simmons
  • AFFINITY PEPTIDES AND METHOD FOR PURIFICATION OF RECOMBINANT PROTEINS
    Publication number: 20120157659
    Abstract: This invention describes a process for separating a fusion protein or polypeptide in the form of its precursor from a mixture containing said fusion protein and impurities, which comprises contacting said fusion protein with a resin containing immobilized metal ions, said fusion protein covalently operably linked directly or indirectly to an immobilized metal ion-affinity peptide, binding said fusion protein to said resin, and selectively eluting said fusion protein from said resin.
    Type: Application
    Filed: July 23, 2010
    Publication date: June 21, 2012
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Ronald A. Hernan, Richard J. Mehigh, Ian R. Brockie, Elizabeth Jenkins
  • GENOME EDITING OF GENES INVOLVED IN ADME AND TOXICOLOGY IN ANIMALS
    Publication number: 20120159654
    Abstract: The present invention provides genetically modified animals and cells comprising edited chromosomal sequences involved in ADME and toxicology. In particular, the animals or cells are generated using a zinc finger nuclease-mediated editing process. The invention also provides zinc finger nucleases that target chromosomal sequence involved in ADME and toxicology and the nucleic acids encoding said zinc finger nucleases. Also provided are methods of assessing the effects of agents in genetically modified animals and cells comprising edited chromosomal sequences involved in ADME and toxicology.
    Type: Application
    Filed: July 23, 2010
    Publication date: June 21, 2012
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Edward Weinstein, Xiaoxia Cui, Phil Simmons
  • High stability diionic liquid salts
    Patent number: 8182581
    Abstract: The present invention relates to diionic liquid salts of dicationic or dianionic molecules, as well as solvents comprising diionic liquids and the use of diionic liquids as the stationary phase in a gas chromatographic column.
    Type: Grant
    Filed: July 22, 2005
    Date of Patent: May 22, 2012
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Daniel W. Armstrong, Jared Anderson
  • GREENER METHOD FOR THE PRODUCTION OF COPOLYMER 1
    Publication number: 20120123094
    Abstract: The present invention provides a greener method for the production of polyamino acid random copolymers containing alanine, glutamic acid, lysine and tyrosine. In particular, the present invention provides a greener method for the production of Copolymer 1 or a pharmaceutically acceptable salt thereof via a synthetic route that only requires a single deprotection step.
    Type: Application
    Filed: November 14, 2011
    Publication date: May 17, 2012
    Applicant: SIGMA-ALDRICH CO. LLC.
    Inventor: Ettigounder Ponnusamy
  • High stability diionic liquid salts
    Patent number: 8168830
    Abstract: The present invention relates to diionic liquid salts of dicationic or dianionic molecules, as well as solvents comprising such diionic liquids and the use of such diionic liquids as the stationary phase in a gas chromatographic column.
    Type: Grant
    Filed: January 31, 2007
    Date of Patent: May 1, 2012
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Daniel W. Armstrong, Jared Anderson
  • HIGH STABILITY POLYIONIC LIQUID SALTS
    Publication number: 20120083603
    Abstract: Polyionic liquid salts are provided comprising polycationic or polyanionic molecules. Further provided are solvents comprising one or more polyionic liquid salts, and the use of such polyionic liquid salts as stationary phases in gas chromatography, and as a reagent in electrospray ionization-mass spectrometry (ESI-MS).
    Type: Application
    Filed: December 13, 2011
    Publication date: April 5, 2012
    Applicant: Sigma-Aldrich Co. LLC
    Inventors: Daniel W. Armstrong, Junmin Huang
  • METHOD FOR THE PRODUCTION OF POLYAMINO ACID RANDOM COPOLYMERS
    Publication number: 20120071612
    Abstract: The present invention provides a method for the production of polyamino acid copolymers. In particular, the method may be utilized to produce high quality polyamino acid random copolymers.
    Type: Application
    Filed: March 8, 2010
    Publication date: March 22, 2012
    Applicant: SIGMA-ALDRICH CO., LLC
    Inventor: Ettigounder Ponnusamy
  • Process for the synthesis of oligonucleotides
    Patent number: 8138330
    Abstract: The present invention discloses novel methods for the synthesis of oligonucleotides with nucleoside phosphoramidites on solid supports. The methods comprise the stepwise chain assembly of oligonucleotides on supports with 5?-acyl phosphoramidites. The synthesis cycles consist of a front end deprotection step which is conducted with a solution of a primary amine or a phenolate, a phosphoramidite coupling step with a 5?-acyl nucleoside phosphoramidite in the presence of an activator, a phosphite oxidation step and an optional capping step. The novel methods improve the quality of synthetic oligonucleotides due to the irreversibility of the front end deprotection step, which prevents the formation of deletion sequences, and due to the avoidance of acidic reagents in the synthesis cycles, which prevent the formation of depurination side products.
    Type: Grant
    Filed: September 11, 2007
    Date of Patent: March 20, 2012
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Michael Leuck, Andreas Wolter, Alfred Stumpe
  • PROCESS THE SYNTHESIS OF BETA GLYCEROL PHOSPHATE
    Publication number: 20120041222
    Abstract: The present invention provides methods for the preparation of beta glycerol phosphate and its salts. In particular, the invention provides efficient methods for the synthesis of beta glycerol phosphate of high purity.
    Type: Application
    Filed: January 8, 2010
    Publication date: February 16, 2012
    Applicant: SIGMA-ALDRICH CO.
    Inventor: Yashwant Kulkarni
  • GENOMIC EDITING OF GENES INVOLVED WITH PARKINSONS DISEASE
    Publication number: 20120030778
    Abstract: The present invention provides genetically modified animals and cells comprising edited chromosomal sequences encoding proteins associated with Parkinson's disease. In particular, the animals or cells are generated using a zinc finger nuclease-mediated editing process. Also provided are methods of assessing the effects of agents in genetically modified animals and cells comprising edited chromosomal sequences encoding proteins associated with Parkinson's disease.
    Type: Application
    Filed: July 27, 2011
    Publication date: February 2, 2012
    Applicant: SIGMA-ALDRICH CO., LLC.
    Inventors: Edward Weinstein, Phil Simmons
  • GENOME EDITING OF CYTOCHROME P450 IN ANIMALS
    Publication number: 20120023599
    Abstract: The present invention provides genetically modified animals and cells comprising edited chromosomal sequences encoding cytochrome P450 (CYP) proteins. In particular, the animals or cells are generated using a zinc finger nuclease-mediated editing process. The invention also provides zinc finger nucleases that target chromosomal sequence encoding CYP proteins, as well as methods of using the genetically modified animals or cells disclosed herein to screen agents for toxicity and other effects.
    Type: Application
    Filed: July 23, 2010
    Publication date: January 26, 2012
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Edward Weinstein, Xiaoxia Cui, Victoria Brown-Kennerly
  • High stability polyionic liquid salts
    Patent number: 8097721
    Abstract: Polyionic liquid salts are provided comprising polycationic or polyanionic molecules. Further provided are solvents comprising one or more polyionic liquid salts, and the use of such polyionic liquid salts as stationary phases in gas chromatography, and as a reagent in electrospray ionization-mass spectrometry (ESI-MS).
    Type: Grant
    Filed: January 31, 2008
    Date of Patent: January 17, 2012
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Daniel W. Armstrong, Junmin Huang
  • OXAZINE DYES WITH IMPROVED AQUEOUS SOLUBILITY
    Publication number: 20120010404
    Abstract: The present invention provides oxazine dyes, processes for preparing the oxazine dyes, intermediates that may be utilized in the processes for preparing the oxazine dyes, and methods for using the oxazine dyes.
    Type: Application
    Filed: June 4, 2009
    Publication date: January 12, 2012
    Applicant: SIGMA-ALDRICH CO.
    Inventor: Alexei Toutchkine
  • Polysilazane thermosetting polymers for use in chromatographic systems and applications
    Patent number: 8092770
    Abstract: This invention relates to an amorphous non-glassy ceramic composition that may be prepared by curing, calcining and/or pyrolyzing a precursor material comprising silicon, a Group III metal, a Group IVA metal, and/or a Group IVB metal.
    Type: Grant
    Filed: September 29, 2008
    Date of Patent: January 10, 2012
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: William R. Betz, Christopher M. Linton
  • Polysilazane thermosetting polymers for use in chromatographic systems and applications
    Patent number: 8088350
    Abstract: This invention relates to an amorphous non-glassy ceramic composition that may be prepared by curing, calcining and/or pyrolyzing a precursor material comprising silicon, a Group III metal, a Group IVA metal, and/or a Group IVB metal.
    Type: Grant
    Filed: February 8, 2008
    Date of Patent: January 3, 2012
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: William R. Betz, Christopher M. Linton
  • STABILIZED COMPOSITIONS OF THERMOSTABLE DNA POLYMERASE AND ANIONIC OR ZWITTERIONIC DETERGENT
    Publication number: 20110312035
    Abstract: The present invention provides compositions, methods, and kits for protecting thermostable DNA polymerase during amplification reactions conducted at a temperature ranging from about 40° C. to greater than 100° C. The composition comprises a thermostable DNA polymerase and an anionic detergent or zwitterionic detergent.
    Type: Application
    Filed: April 22, 2011
    Publication date: December 22, 2011
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Brian W. Ward, Ernest J. Mueller, Jessica Copeland, Deborah Vassar