Patents Assigned to Sigma-Aldrich
  • Methods of forming thin metal-containing films by chemical phase deposition
    Patent number: 8481121
    Abstract: Methods of forming thin metal-containing films by chemical phase deposition, particularly atomic layer deposition (ALD) and chemical vapor deposition (CVD), are provided. The methods comprise delivering at least one organometallic precursor to a substrate, wherein the at least one precursor corresponds in structure to Formula (II); wherein: M is Ru, Fe or Os; R is Q-C10-alkyl; X is C1-C10-alkyl; and n is zero, 1, 2, 3, 4 or 5. Further provided are methods of making precursors disclosed herein.
    Type: Grant
    Filed: July 24, 2008
    Date of Patent: July 9, 2013
    Assignee: Sigma-Aldrich Co., LLC
    Inventors: Ravi Kanjolia, Rajesh Odedra, Neil Boag, David Weyburne
  • High stability polyionic liquid salts
    Patent number: 8481722
    Abstract: Polyionic liquid salts are provided comprising polycationic or polyanionic molecules. Further provided are solvents comprising one or more polyionic liquid salts, and the use of such polyionic liquid salts as stationary phases in gas chromatography, and as a reagent in electrospray ionization-mass spectrometry (ESI-MS).
    Type: Grant
    Filed: December 13, 2011
    Date of Patent: July 9, 2013
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Daniel W Armstrong, Junmin Huang
  • CELLS FOR CHROMATIN IMMUNOPRECIPITATION AND METHODS FOR MAKING
    Publication number: 20130172198
    Abstract: The present invention provides isolated, non-viable bacterial cells comprising a plurality of nucleic acid crosslinks, methods for making said cells, and methods for using said cells to isolate DNA-protein complexes. In particular, the nucleic acids of the cells are crosslinked by contacting the cells with a furocoumarin compound and ultraviolet light.
    Type: Application
    Filed: September 2, 2011
    Publication date: July 4, 2013
    Applicant: SIGMA-ALDRICH CO., LLC
    Inventors: Vikas B. Palhan, Carol Kreader, Ernie Mueller
  • PREPARATION OF LOW MOLECULAR WEIGHT POLYORNITHINE IN HIGH YIELD
    Publication number: 20130172517
    Abstract: The present invention generally relates to the large-scale (e.g., greater than 1 kg scale) preparation of low molecular weight polylysine or polyornithine in high yield by preparing a polylysine or polyornithine having a weight average molecular weight from about 12,500 Daltons to about 22,000 Daltons and hydrolyzing it to produce a polylysine or polyornithine having a weight average molecular weight from about 5,500 Daltons to about 12,000 Daltons.
    Type: Application
    Filed: February 5, 2013
    Publication date: July 4, 2013
    Applicant: Sigma-Aldrich Co. LLC
    Inventor: Ettigounder Ponnusamy
  • Organometallic precursors for use in chemical phase deposition processes
    Patent number: 8476467
    Abstract: An organometallic precursor is provided. The precursor corresponds in structure to Formula I: Cp(R)nM(CO)2(X)??(Formula I) wherein: M is Ru, Fe or Os; R is C1-C10-alkyl; X is C1-C10-alkyl; and n is 1, 2, 3, 4 or 5. The precursors are useful in chemical phase deposition processes, such as atomic layer deposition (ALD) and chemical vapor deposition (CVD).
    Type: Grant
    Filed: July 24, 2008
    Date of Patent: July 2, 2013
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Ravi Kanjolia, Rajesh Odedra, Neil Boag
  • PROCESS FOR CONJUGATION OF NHS ESTERS WITH OLIGONUCLEOTIDES
    Publication number: 20130158243
    Abstract: The present invention provides processes for the conjugation of NHS esters to amino-modified oligonucleotides. The processes provide the amino-modified oligonucleotide on a solid support such that conjugation can be carried out under conditions that can accommodate a wide variety of NHS esters and oligonucleotides.
    Type: Application
    Filed: December 19, 2012
    Publication date: June 20, 2013
    Applicant: Sigma-Aldrich Co. LLC
    Inventor: Robert William Ball
  • GENOME EDITING USING TARGETING ENDONUCLEASES AND SINGLE-STRANDED NUCLEIC ACIDS
    Publication number: 20130137180
    Abstract: The present invention provides methods and kits for editing specific chromosomal sequences in cells. In particular, targeting endonucleases and single-stranded nucleic acids are used to edit the chromosomal sequence.
    Type: Application
    Filed: July 22, 2011
    Publication date: May 30, 2013
    Applicant: SIGMA ALDRICH CO, LLC
    Inventors: Fuqiang Chen, Shondra M. Pruett-Miller, Gregory D. Davis
  • Process for the synthesis of beta glycerol phosphate
    Patent number: 8420848
    Abstract: The present invention provides methods for the preparation of beta glycerol phosphate and its salts. In particular, the invention provides efficient methods for the synthesis of beta glycerol phosphate of high purity.
    Type: Grant
    Filed: January 8, 2010
    Date of Patent: April 16, 2013
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Yashwant Kulkarni
  • METHODS FOR QUANTITATING WATER USING IONIC LIQUID SALTS
    Publication number: 20130074580
    Abstract: This disclosure provides methods and devices for quantitating, separating and/or detecting water in a liquid, gas or solid sample comprising one or more chemicals, the method comprising: providing the liquid, gas or solid sample comprising water and the one or more chemicals; and exposing said liquid, gas or solid sample to at least one solid support including at least one dicationic and/or tricationic species of Formula I or II adsorbed, absorbed or immobilized on the solid support.
    Type: Application
    Filed: September 24, 2012
    Publication date: March 28, 2013
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventor: SIGMA-ALDRICH CO. LLC
  • Methods of using stabilized compositions of thermostable DNA polymerase and anionic detergent
    Patent number: 8404464
    Abstract: The present invention provides compositions, methods, and kits for protecting thermostable DNA polymerase during amplification reactions conducted at a temperature ranging from about 40° C. to greater than 100° C. The composition comprises a thermostable DNA polymerase and an anionic detergent or zwitterionic detergent.
    Type: Grant
    Filed: April 22, 2011
    Date of Patent: March 26, 2013
    Assignee: Sigma-Aldrich Co., LLC
    Inventors: Brian W. Ward, Ernest J. Mueller, Jessica Copeland, Deborah Vassar
  • Preparation of low molecular weight polylysine and polyornithine in high yield
    Patent number: 8399600
    Abstract: The present invention generally relates to the large-scale (e.g., greater than 1 kg scale) preparation of low molecular weight polylysine or polyornithine in high yield by preparing a polylysine or polyornithine having a weight average molecular weight from about 12,500 Daltons to about 22,000 Daltons and hydrolyzing it to produce a polylysine or polyornithine having a weight average molecular weight from about 5,500 Daltons to about 12,000 Daltons. In preferred embodiments, the polymer is a homopolymer of poly-L-lysine.
    Type: Grant
    Filed: July 29, 2009
    Date of Patent: March 19, 2013
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Ettigounder Ponnusamy
  • USE OF ENDOGENOUS PROMOTERS TO EXPRESS HETEROLOGOUS PROTEINS
    Publication number: 20130065310
    Abstract: The present invention provides methods for using endogenous transcriptional control systems to regulate the expression of heterologous protein(s). In particular, targeted genome editing is used to integrate a sequence encoding the heterologous protein(s) in-frame with an endogenous coding sequence such that the expression of the heterologous and endogenous sequences is regulated by the endogenous control system.
    Type: Application
    Filed: April 13, 2011
    Publication date: March 14, 2013
    Applicant: SIGMA-ALDRICH CO., LLC
    Inventors: Greg Davis, Dmitry Malkov, Nathan Zenser
  • SYSTEMS AND METHODS FOR VALIDATING AND CUSTOMIZING OLIGONUCLEOTIDES SEQUENCES
    Publication number: 20130060534
    Abstract: A system and method is provided to customize and validate an oligonucleotide plate and/or tube design. A graphical user interface enables a user to submit oligo design data in a series of steps. One or more rules are used to validate the oligo plate design data as it is submitted. The graphical user interface also enables the user to modify oligonucleotide design data to correct errors and/or to obtain a valid oligonucleotide plate design.
    Type: Application
    Filed: August 30, 2012
    Publication date: March 7, 2013
    Applicant: SIGMA-ALDRICH CO., LLC
    Inventors: Michael Scott Sample, Stacey Hoge, Parth Bhawalkar, Simon Blackwell, Richard Moloney, Tina Rodgers, Amber Walters
  • BIOSENSORS COMPRISING PROTEIN-BINDING DOMAINS AND FLUORESCENT PROTEINS
    Publication number: 20130059362
    Abstract: The present invention provides compositions and methods for monitoring cellular signal transduction events. In particular, signal transduction events may be monitored by the use of biosensors comprising protein-binding domains, which bind signal-transduction proteins, and fluorescent proteins. Provided herein, therefore, methods for detecting activation of signal transduction proteins or screening for agents that modulate the activity of signal transduction proteins. Also provided are cells comprising the biosensors; lentiviral particles comprising biosensor coding sequence; and kits comprising the lentiviral particles.
    Type: Application
    Filed: April 13, 2011
    Publication date: March 7, 2013
    Applicant: SIGMA-ALDRICH CO., LLC
    Inventors: John Fetter, Dmitry Malkov, Nathan Zenser, Keming Song
  • METHODS FOR GENERATING ENDOGENOUSLY TAGGED PROTEINS
    Publication number: 20130059388
    Abstract: The present disclosure provides a method for endogenously tagging an endogenous protein in a cell, and a cell comprising an endogenously tagged protein. Also described are cells produced using such a method and a kit comprising a cell having tagged endogenous protein.
    Type: Application
    Filed: April 13, 2011
    Publication date: March 7, 2013
    Applicant: SIGMA-ALDRICH CO., LLC
    Inventors: Dmitry Malkov, Nathan Zenser, Deborah Vassar, Fan Zhang, Hongyi Zhang
  • METHODS FOR PREPARING THIN FILMS BY ATOMIC LAYER DEPOSITION USING HYDRAZINES
    Publication number: 20130052368
    Abstract: A method of forming a metal-containing film by atomic layer deposition is provided herein. The method comprises using (a) at least one metal fluorinated ?-diketonate precursor; and (b) a co-reagent comprising at least one optionally-substituted hydrazine.
    Type: Application
    Filed: March 14, 2011
    Publication date: February 28, 2013
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Simon Rushworth, Paul Williams
  • ALKYL-SUBSTITUTED ALLYL CARBONYL METAL COMPLEXES AND USE THEREOF FOR PREPARING DIELECTRIC THIN FILMS
    Publication number: 20130041170
    Abstract: Organometallic complexes and use thereof in thin film deposition, such as CVD and ALD are provided herein. The organometallic complexes are (alkyl-substituted ?3-allyl)(carbonyl)metal complexes.
    Type: Application
    Filed: August 8, 2012
    Publication date: February 14, 2013
    Applicant: Sigma-Aldrich Co. LLC
    Inventors: Rajesh Odedra, Neil Boag, Jeff Anthis, Ravi Kanjolia, Mark Saly
  • Calibration system and dye kit and their uses for characterizing luminescence measurement systems
    Patent number: 8361394
    Abstract: A calibration system characterizes luminescence measurement systems, in particular spectrally resolving, wide-field and/or confocal imaging systems. The calibration system has a baseplate with at least one flow-through channel, wherein the at least one channel is formed as a sample chamber for the luminescence measurement system, at least one reservoir in communication with the at least one channel and adapted to receive a liquid, and at least one focusing device integrated into a baseplate for setting a defined measurement beam focus of the luminescence measurement system to be calibrated by using a focusing surface.
    Type: Grant
    Filed: March 17, 2006
    Date of Patent: January 29, 2013
    Assignees: Bam Bundesanstalf Fuer Materialforschung und - Pruefung, Sigma-Aldrich GmbH
    Inventors: Ute Resch-Genger, Katrin Hoffmann, Dietmar Pfeifer, Roland Nitschke, Pierre Nording
  • CELLS DEFICIENT IN CMP-N-ACETYLNEURAMINIC ACID HYDROXYLASE AND/OR GLYCOPROTEIN ALPHA-1,3-GALACTOSYLTRANSFERASE
    Publication number: 20130004992
    Abstract: The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation.
    Type: Application
    Filed: June 29, 2012
    Publication date: January 3, 2013
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Nan Lin, Henry J. George, Joaquina Mascarenhas, Trevor N. Collingwood, Kevin J. Kayser, Katherine Achtien
  • METAL AMIDOBORANE COMPOSITIONS AND PROCESSES FOR THEIR PREPARATION
    Publication number: 20120301382
    Abstract: The present invention provides compositions comprising a metal amidoborane and an amine, and processes for preparing the metal amidoborane compositions. In particular, the process comprises contacting ammonia borane with a metal amide in the presence of an amine solvent to form the metal amidoborane composition. The invention also provides methods for generating hydrogen, wherein the method comprises heating the metal amidoborane composition such that hydrogen is released.
    Type: Application
    Filed: April 13, 2010
    Publication date: November 29, 2012
    Applicant: SIGMA ALDRICH CO. LLC
    Inventors: Viktor Balema, Kanth Josyula, Guolin Xu, Nathaniel Wallock, Scott Batcheller, Peng Gao, Shashi Jasty