Patents Assigned to SPIBER INC.
  • Patent number: 11142553
    Abstract: The present invention relates to a protein composition formed of dry matter of a dispersion in which a protein is dispersed in a stabilizer solution, in which the stabilizer includes at least one type selected from the group consisting of a catechol-based stabilizer, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, 2,6-di-tert-butyl-p-cresol, 2-tert-butyl-4-methoxyphenol, and 6,6?-di-tert-butyl-4,4?-butylidenedi-m-cresol.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: October 12, 2021
    Assignees: Spiber Inc., Kojima Industries Corporation
    Inventors: Toshiaki Taniike, Anh Thi Ngoc Dao, Koyuru Nakayama, Junichi Shimokata, Kengo Takeuchi, Abhijit Ravikiran Muley, Sunita Darbe
  • Publication number: 20210292752
    Abstract: Disclosed is a method for isolating or identifying target clone cells from a cell population, the method including steps of: preparing a cell population into which a barcode sequence and at least one reporter protein abnormal expression cassette linked to the barcode sequence are introduced; introducing a barcode sequence recognition module targeting an arbitrary barcode sequence and a nucleic acid mutation repair enzyme into cells; repairing a nucleic acid mutation causing abnormal expression occurring in the at least one reporter protein abnormal expression cassette by expression of a complex of the barcode sequence recognition module and the nucleic acid mutation repair enzyme in a cell containing the target barcode sequence, to induce normal expression of the reporter protein; and isolating or identifying target clone cells in which the reporter protein is expressed.
    Type: Application
    Filed: August 13, 2019
    Publication date: September 23, 2021
    Applicants: Spiber Inc., The University of Tokyo
    Inventors: Kana Ishida, Soh Ishiguro, Nozomu Yachie, Chikako Sato, Junichi Sugahara
  • Publication number: 20210230424
    Abstract: Provided is a flexible polyurethane foam having high hardness required for weight reduction of members and having low stress relaxation. A flexible polyurethane foam comprises a structural protein fiber having a length of 0.1 mm to 5 mm.
    Type: Application
    Filed: July 5, 2019
    Publication date: July 29, 2021
    Applicants: BRIDGESTONE CORPORATION, SPIBER INC.
    Inventors: Koji SANEFUJI, Hideharu SEGUCHI, Toshiki TAKIZAWA, Naoko NISIKITA
  • Publication number: 20210214404
    Abstract: The present invention pertains to an artificial hair fiber that includes an artificial fibroin fiber containing a modified fibroin and that expands when being in a wet state and shrinks when being dried after the wet state.
    Type: Application
    Filed: July 24, 2019
    Publication date: July 15, 2021
    Applicants: Spiber Inc., Kojima Industries Corporation
    Inventors: Masato Matsuo, Hideto Ishii, Yunosuke Abe
  • Publication number: 20210162677
    Abstract: Disclosed is a molded article including a modified fibroin and a water resistance-imparting material. The modified fibroin and the water resistance-imparting material may be covalently bonded.
    Type: Application
    Filed: April 1, 2019
    Publication date: June 3, 2021
    Applicant: Spiber Inc.
    Inventors: Kazuki Sakata, Kentaro Takahashi
  • Patent number: 10975206
    Abstract: A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamine acid is dissolved in a polar solvent. An inorganic salt is added to the solution, and a mole ratio of moisture to the inorganic salt in the solution is 2.5×m×n or less, where m represents the number of cations forming the inorganic salt and n represents a charge number of the cation. A production method of the present invention is a method for producing the above solution, including: changing at, least one of a moisture content and a content of the inorganic salt in the solution to adjust a viscosity of the solution. Thus, the present invention provides a polar solvent solution whose viscosity can be easily adjusted to a desired value and thus enables stable spinning and casting when used as dopes for spinning, film, etc., and methods for producing the same.
    Type: Grant
    Filed: April 4, 2016
    Date of Patent: April 13, 2021
    Assignee: SPIBER INC.
    Inventors: Kana Ishida, Hironori Yamamoto, Hiroaki Suzumura, Kazuhide Sekiyama
  • Publication number: 20210095399
    Abstract: Provided is a side-by-side type composite fiber having a latent crimping ability, including a first component containing a modified fibroin and a second component containing a structural protein, in which the first component and the second component are joined to each other.
    Type: Application
    Filed: April 3, 2019
    Publication date: April 1, 2021
    Applicant: Spiber Inc.
    Inventors: Yunosuke Abe, Shota Togashi
  • Patent number: 10961285
    Abstract: Provided is a molded article of a composition comprising a polypeptide, wherein the polypeptide is at least one kind selected from the group consisting of natural spider silk protein and polypeptides derived from natural spider silk protein.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: March 30, 2021
    Assignees: Spiber Inc., Kojima Industries Corporation
    Inventors: Junichi Sugahara, Kaori Sekiyama, Ayumi Abe, Junichi Shimokata, Junichi Noba, Shinji Hirai
  • Publication number: 20210040649
    Abstract: An object of the present invention is to provide a method for efficiently manufacturing a protein crimped staple from protein filaments at a low cost. A manufacturing method for a protein crimped staple according to the present invention includes: a) preparing an artificial fibroin filament containing a modified fibroin; b) cutting the artificial fibroin filament to obtain an artificial fibroin staple; and c) performing crimping by bringing the artificial fibroin filament into contact with an aqueous medium to crimp the artificial fibroin filament before the cutting or bringing the artificial fibroin staple into contact with an aqueous medium to crimp the artificial fibroin staple after the cutting.
    Type: Application
    Filed: January 31, 2019
    Publication date: February 11, 2021
    Applicant: Spiber Inc.
    Inventors: Akito Sato, Yunosuke Abe
  • Publication number: 20210040648
    Abstract: The present invention relates to a method for manufacturing a protein fiber, the method including a step of bringing a spinning raw liquid containing a spider silk protein and a solvent into contact with a coagulation liquid to coagulate the spider silk protein, in which the coagulation liquid contains a ketone.
    Type: Application
    Filed: January 31, 2019
    Publication date: February 11, 2021
    Applicant: Spiber Inc.
    Inventors: Yunosuke Abe, Hirotada Ando
  • Publication number: 20210032778
    Abstract: The present invention relates to a production method for a protein molded article, including: dissolving a protein in a solvent containing formic acid at a temperature of 40° C. or higher and lower than 80° C. to obtain a protein solution; and molding a protein molded article using the protein solution.
    Type: Application
    Filed: January 31, 2019
    Publication date: February 4, 2021
    Applicant: Spiber Inc.
    Inventors: Takuya Saito, Ryoji Okada, Naoki Matsuzaka
  • Publication number: 20210032782
    Abstract: The present invention aims at providing a blended yarn with which a knitted or woven body, wherein a decrease in tension and stiffness due to moistness is reduced, can be manufactured, even in a case where the blended yarn is used for the manufacture of the knitted or woven body; a knitted or woven body of the same; and a method for manufacturing the knitted or woven body. The blended yarn according to the present invention comprises a modified fibroin fiber and a regenerated cellulose fiber.
    Type: Application
    Filed: April 3, 2019
    Publication date: February 4, 2021
    Applicants: Hasetora Spinning Co., Ltd., Spiber Inc.
    Inventors: Tetsuhiro Asano, Akihiko Ozeki
  • Patent number: 10899792
    Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: January 26, 2021
    Assignee: Spiber Inc.
    Inventors: Toshiaki Osawa, Yuya Sato, Keisuke Morita
  • Publication number: 20200339640
    Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the cell proliferation after inducing the expression is reduced.
    Type: Application
    Filed: July 15, 2020
    Publication date: October 29, 2020
    Applicants: AJINOMOTO CO., INC., SPIBER INC.
    Inventor: Kentaro Nakase
  • Publication number: 20200308552
    Abstract: Disclosed is an expression cassette comprising, in a 5? to 3? direction of a sense strand, a promoter, and a first nucleic acid, a terminator and a second nucleic acid operably linked to the promoter, wherein the first nucleic acid and the second nucleic acid each contain at least one gene.
    Type: Application
    Filed: September 28, 2018
    Publication date: October 1, 2020
    Applicant: Spiber Inc.
    Inventor: Masahiro Murakami
  • Patent number: 10752660
    Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the cell proliferation after inducing the expression is reduced.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: August 25, 2020
    Assignees: AJINOMOTO CO., INC., SPIBER INC.
    Inventor: Kentaro Nakase
  • Publication number: 20200248231
    Abstract: The present invention relates to a method for producing a double-stranded DNA fragment having a desired nucleotide sequence through dual asymmetric PCR (DA-PCR). The method comprises: (1) providing a plurality of oligonucleotides (sense oligonucleotides) each corresponding to a part of a sense strand of the double-stranded DNA fragment and a plurality of oligonucleotides (antisense oligonucleotides) each corresponding to a part of an antisense strand of the double-stranded DNA fragment and mixing together the oligonucleotides with equal concentrations, DNA polymerase, and dNTP to prepare a reaction mixture solution; (2) performing PCR by using the reaction mixture solution from step (1); (3) adding a primer set capable of amplifying the double-stranded DNA fragment of full length to the reaction mixture solution from step (2); and (4) performing PCR by using the reaction mixture solution from step (3).
    Type: Application
    Filed: August 28, 2018
    Publication date: August 6, 2020
    Applicant: Spiber Inc.
    Inventors: Yusuke Ito, Chikako Sato, Nozomu Yachie
  • Publication number: 20200207817
    Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m or Formula 2: [(A)n motif-REP]m-(A)n motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin. [In Formula 1 and Formula 2, (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues, and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300. A plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences.
    Type: Application
    Filed: July 25, 2018
    Publication date: July 2, 2020
    Applicants: Spiber Inc., Riken
    Inventors: Keisuke Morita, Yunosuke Abe, Takehisa Maekawa, Koichi Kotaka, Junichi Sugahara, Keiji Numata
  • Publication number: 20200165311
    Abstract: The present invention relates to a polypeptide solution in which polypeptide including an amino acid sequence derived from type I keratin that belongs to Cluster Ia is dissolved in a solvent including a formic acid or a solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt.
    Type: Application
    Filed: May 9, 2018
    Publication date: May 28, 2020
    Applicants: Spiber Inc., Kojima Industries Corporation
    Inventors: Ayumi Abe, Keisuke Morita, Kenji Kurachi, Yunosuke Abe
  • Publication number: 20200130335
    Abstract: A composite material 100 is obtained by laminating and tightly adhering a fabric 60 containing fibers made of spider silk fibroin and a synthetic resin film 50 having a tensile elongation equal to or greater than that of the fabric 60.
    Type: Application
    Filed: July 3, 2018
    Publication date: April 30, 2020
    Applicants: Spiber Inc., Kojima Industries Corporation
    Inventors: Kazuhiro Uchida, Masahiro Asakawa, Hiroyuki Mori, Wataru Ishida, Shinya Ueda